Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,404,059 C→A 100% R141S (CGC→AGC)  dusB → tRNA‑dihydrouridine synthase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,404,0590CA96.9% 610.7 / 6.1 194R141S (CGC→AGC) dusBtRNA‑dihydrouridine synthase B
Reads supporting (aligned to +/- strand):  ref base C (4/2);  new base A (106/82);  total (110/84)
Fisher's exact test for biased strand distribution p-value = 7.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 194 total aligned reads displayed.

GATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAG  >  CP009273/3403914‑3404203
                                                                                                                                                 |                                                                                                                                                
gATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGc                                                                                                                                                >  1:1117387/1‑148 (MQ=255)
gATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGc                                                                                                                                                >  1:890130/1‑148 (MQ=255)
 attattGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCa                                                                                                                                               <  1:2391915/148‑1 (MQ=255)
    attGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCg                                                                                                                                            >  1:2214070/1‑148 (MQ=255)
     ttGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCgg                                                                                                                                           >  1:3277142/1‑148 (MQ=255)
     ttGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCgg                                                                                                                                           >  1:1592862/1‑148 (MQ=255)
      tGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGc                                                                                                                                          >  1:1594623/1‑148 (MQ=255)
         tatCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTgg                                                                                                                                       >  2:939777/1‑148 (MQ=255)
         tatCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTgg                                                                                                                                       >  2:2062247/1‑148 (MQ=255)
                atGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCgg                                                                                                                                >  2:1533152/1‑148 (MQ=255)
                   ggTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAac                                                                                                                             >  2:2876815/1‑148 (MQ=255)
                    gTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAaca                                                                                                                            >  2:2527036/1‑148 (MQ=255)
                          cGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTaa                                                                                                                      <  1:7596/148‑1 (MQ=255)
                          cGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTaa                                                                                                                      <  1:281257/148‑1 (MQ=255)
                          cGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTaa                                                                                                                      <  1:1832901/148‑1 (MQ=255)
                           ggCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAAc                                                                                                                     <  1:1120766/148‑1 (MQ=255)
                           ggCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCATCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAAc                                                                                                                     <  1:455177/148‑1 (MQ=255)
                                   aaGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAaga                                                                                                             >  2:22212/1‑148 (MQ=255)
                                   aaGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAaga                                                                                                             >  2:168318/1‑148 (MQ=255)
                                   aaGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAaga                                                                                                             >  2:2811854/1‑148 (MQ=255)
                                     gTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAgaga                                                                                                           >  2:1773303/1‑148 (MQ=255)
                                     gTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAgaga                                                                                                           <  2:1863341/148‑1 (MQ=255)
                                      tGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGAt                                                                                                          >  1:1353456/1‑148 (MQ=255)
                                       gAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGAtt                                                                                                         >  2:2595330/1‑148 (MQ=255)
                                          tCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGcc                                                                                                      >  2:572742/1‑148 (MQ=255)
                                           cGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGATGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGccc                                                                                                     <  2:2807606/148‑1 (MQ=255)
                                             cAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCaa                                                                                                   <  2:2461693/148‑1 (MQ=255)
                                             cAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCaa                                                                                                   <  2:652086/148‑1 (MQ=255)
                                                 cTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTgg                                                                                               >  2:3536150/1‑148 (MQ=255)
                                                  tCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGc                                                                                              <  2:1739773/148‑1 (MQ=255)
                                                       ggTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAg                                                                                         <  1:602385/148‑1 (MQ=255)
                                                        gTTCAGCCCTCTTGCAGTACCCAGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGa                                                                                        <  2:2224762/148‑1 (MQ=255)
                                                         ttCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGAc                                                                                       >  2:2896308/1‑148 (MQ=255)
                                                         ttCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGAc                                                                                       <  2:3195762/148‑1 (MQ=255)
                                                           cAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACtg                                                                                     <  1:1929702/148‑1 (MQ=255)
                                                             gCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACtgtg                                                                                   >  1:2469699/1‑148 (MQ=255)
                                                             gCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACtgtg                                                                                   >  2:177601/1‑148 (MQ=255)
                                                              cccTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTgg                                                                                  >  2:3453588/1‑148 (MQ=255)
                                                                ctctTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCa                                                                                >  2:2726204/1‑148 (MQ=255)
                                                                  ctTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCAtt                                                                              >  2:3311981/1‑148 (MQ=255)
                                                                   ttGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTc                                                                             <  1:152587/148‑1 (MQ=255)
                                                                    tGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCa                                                                            <  2:856451/148‑1 (MQ=255)
                                                                    tGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCa                                                                            <  1:2040555/148‑1 (MQ=255)
                                                                        gTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCTGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGct                                                                        >  1:1091388/1‑148 (MQ=255)
                                                                        gTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGct                                                                        >  2:1227734/1‑148 (MQ=255)
                                                                         tACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTCCCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGctc                                                                       >  1:3304978/1‑148 (MQ=255)
                                                                         tACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGctc                                                                       >  1:3146177/1‑148 (MQ=255)
                                                                                  gTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTc                                                              >  1:3501803/1‑148 (MQ=255)
                                                                                  gTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTc                                                              <  1:1647/148‑1 (MQ=255)
                                                                                       tAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGc                                                         >  2:1649488/1‑148 (MQ=255)
                                                                                       tAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGc                                                         >  2:3215645/1‑148 (MQ=255)
                                                                                          aTCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGt                                                      <  1:2976440/148‑1 (MQ=255)
                                                                                          aTCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGt                                                      <  1:2533929/148‑1 (MQ=255)
                                                                                           tCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTa                                                     >  2:3258389/1‑148 (MQ=255)
                                                                                              aTCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTacac                                                  >  1:1984911/1‑148 (MQ=255)
                                                                                              aTCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTacac                                                  >  2:1877119/1‑148 (MQ=255)
                                                                                              aTCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTacac                                                  <  1:2956921/148‑1 (MQ=255)
                                                                                                ccTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACAcgc                                                >  2:2265073/1‑148 (MQ=255)
                                                                                                ccTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACAcgc                                                >  2:1658209/1‑148 (MQ=255)
                                                                                                  ttACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACgcgc                                              <  2:2340777/148‑1 (MQ=255)
                                                                                                   tACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGcc                                             >  2:605103/1‑148 (MQ=255)
                                                                                                   tACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGcc                                             >  2:604575/1‑148 (MQ=255)
                                                                                                   tACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGcc                                             >  1:614468/1‑148 (MQ=255)
                                                                                                    aCCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCt                                            >  2:2183923/1‑148 (MQ=255)
                                                                                                      cGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCtgt                                          <  2:787020/148‑1 (MQ=255)
                                                                                                        aGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCtgttt                                        <  2:2051562/148‑1 (MQ=255)
                                                                                                         ggTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCtgtttg                                       >  1:1422163/1‑148 (MQ=255)
                                                                                                         ggTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCtgtttg                                       >  1:1179832/1‑148 (MQ=255)
                                                                                                           tcgtcAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCtgtttgtt                                     <  2:1326764/148‑1 (MQ=255)
                                                                                                            cgtcAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTc                                    >  2:1186866/1‑148 (MQ=255)
                                                                                                            cgtcAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTc                                    <  2:3352917/148‑1 (MQ=255)
                                                                                                            cgtcAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTc                                    <  1:3351741/148‑1 (MQ=255)
                                                                                                              tcAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCaa                                  <  2:1016267/148‑1 (MQ=255)
                                                                                                              tcAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCaa                                  >  1:2626390/1‑148 (MQ=255)
                                                                                                              tcAAGGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCaa                                  <  2:2953344/148‑1 (MQ=255)
                                                                                                               cAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAAt                                 <  2:2978763/148‑1 (MQ=255)
                                                                                                               cAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAAt                                 >  2:3011402/1‑148 (MQ=255)
                                                                                                               cAATGCAGTGGACGTTCCTGTGACCCTGAAGATTAGCACCGGCTGGTCACCGGAACACCGTAACTGCGAAGAGATTGCCCATCTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAAt                                 <  2:2485493/148‑1 (MQ=255)
                                                                                                                 aTGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATgg                               <  1:22226/148‑1 (MQ=255)
                                                                                                                   gCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGgag                             <  2:31846/148‑1 (MQ=255)
                                                                                                                    cAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGgaga                            <  2:488759/148‑1 (MQ=255)
                                                                                                                      gTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAg                          <  2:2786057/148‑1 (MQ=255)
                                                                                                                       tGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGGCTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGc                         >  1:2132417/1‑148 (MQ=255)
                                                                                                                        ggACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTGTGTTCCATGGCGAAGCt                        >  2:1936998/1‑148 (MQ=255)
                                                                                                                            gTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGt                    <  2:1775471/148‑1 (MQ=255)
                                                                                                                             ttCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTa                   >  1:3392630/1‑148 (MQ=255)
                                                                                                                              tCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTAc                  >  2:754882/1‑148 (MQ=255)
                                                                                                                                cTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGa                >  2:1989683/1‑148 (MQ=255)
                                                                                                                                cTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGa                >  2:2105686/1‑148 (MQ=255)
                                                                                                                                  gTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACa              >  1:560013/1‑148 (MQ=255)
                                                                                                                                     aCCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTa           >  2:3388901/1‑148 (MQ=255)
                                                                                                                                     aCCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTa           >  2:1628047/1‑148 (MQ=255)
                                                                                                                                      cccTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTAt          <  1:567533/148‑1 (MQ=255)
                                                                                                                                       ccTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTAtt         >  1:1863397/1‑148 (MQ=255)
                                                                                                                                       ccTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTAtt         <  1:939777/148‑1 (MQ=255)
                                                                                                                                         tGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCg       <  1:3374618/148‑1 (MQ=255)
                                                                                                                                         tGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCg       >  1:1623386/1‑148 (MQ=255)
                                                                                                                                           aaGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCggg     >  1:2038038/1‑148 (MQ=255)
                                                                                                                                             gATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCa   >  1:2885164/1‑148 (MQ=255)
                                                                                                                                              aTTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAg  <  1:1972979/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAG  >  CP009273/3403914‑3404203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑