Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,439,443 G→T 8.7% I19I (ATC→ATA)  rplF ← 50S ribosomal subunit protein L6

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,439,4430GT 8.7% 484.2 / 3.4 175I19I (ATC→ATA) rplF50S ribosomal subunit protein L6
Reads supporting (aligned to +/- strand):  ref base G (83/69);  new base T (3/20);  total (86/89)
Fisher's exact test for biased strand distribution p-value = 2.08e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 175 total aligned reads displayed.

GCGGTACCAGCCTGTGCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGATCAGTCATAACACCTTTAGAGGT  >  CP009273/3439297‑3439589
                                                                                                                                                  |                                                                                                                                                  
 cgGTACCAGCCTGTGCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGAt                                                                                                                                                  <  1:2558336/148‑1 (MQ=255)
    tACCAGCCTGTGCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGAtttt                                                                                                                                               >  2:1450941/1‑148 (MQ=255)
    tACCAGCCTGTGCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGAtttt                                                                                                                                               <  1:1868850/148‑1 (MQ=255)
         gCCTGTGCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACAGTTGATTTTTACGt                                                                                                                                          >  2:2809857/1‑148 (MQ=255)
           cTGTGCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCa                                                                                                                                        <  1:3134615/148‑1 (MQ=255)
            tgtgCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCaa                                                                                                                                       <  2:2354489/148‑1 (MQ=255)
            tgtgCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCaa                                                                                                                                       <  1:2808878/148‑1 (MQ=255)
            tgtgCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCaa                                                                                                                                       <  2:2354406/148‑1 (MQ=255)
            tgtgCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCaa                                                                                                                                       <  1:1112377/148‑1 (MQ=255)
               gCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGc                                                                                                                                    <  1:2289302/148‑1 (MQ=255)
                    aCCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCa                                                                                                                               <  1:1429202/148‑1 (MQ=255)
                     ccGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAg                                                                                                                              <  1:2425428/148‑1 (MQ=255)
                     ccGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAg                                                                                                                              >  2:1310087/1‑148 (MQ=255)
                     ccGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAg                                                                                                                              >  2:3173547/1‑148 (MQ=255)
                     ccGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAg                                                                                                                              >  2:1310460/1‑148 (MQ=255)
                        tCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGaa                                                                                                                           <  2:1557403/148‑1 (MQ=255)
                        tCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTAACTTTGATAGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGaa                                                                                                                           >  2:3316987/1‑148 (MQ=255)
                          tGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGaaca                                                                                                                         <  1:869294/148‑1 (MQ=255)
                           gCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGTATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGaacaa                                                                                                                        <  1:1720828/148‑1 (MQ=255)
                           gCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGTATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGaacaa                                                                                                                        <  1:1724389/148‑1 (MQ=255)
                              tAACCATCACGCGGACCGAAGGTCTGGTTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCTGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGa                                                                                                                     <  1:1430517/148‑1 (MQ=255)
                               aaCCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGAc                                                                                                                    <  2:3096495/148‑1 (MQ=255)
                               aaCCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGAc                                                                                                                    >  2:2599091/1‑148 (MQ=255)
                               aaCCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGAc                                                                                                                    <  2:550896/148‑1 (MQ=255)
                                 ccATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTTCGTCAACGCCGGCAGGAACAACGACCg                                                                                                                  >  2:435360/1‑148 (MQ=255)
                                  cATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTGTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGGCAACGCCGGCAGGAACACCGACCgg                                                                                                                 >  2:2600380/1‑148 (MQ=255)
                                      ccgcgGACCGAAGGTCAGGTTATTATCTGCATGTTTAACTTCAACAGAATCGTTGAGATTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCTTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCt                                                                                                             <  2:2275844/147‑1 (MQ=255)
                                       cgcgGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCtt                                                                                                            >  2:852218/1‑148 (MQ=255)
                                       cgcgGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAAAGCCGGCAGGAACAACGACCGGTGCtt                                                                                                            >  2:2630774/1‑148 (MQ=255)
                                         cgGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTa                                                                                                          >  2:2872155/1‑148 (MQ=255)
                                          ggACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAg                                                                                                         <  2:495604/148‑1 (MQ=255)
                                          ggACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTGGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAg                                                                                                         <  2:1957060/148‑1 (MQ=255)
                                            aCCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCa                                                                                                       >  2:2925261/1‑148 (MQ=255)
                                             ccGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCaa                                                                                                      <  2:1773464/148‑1 (MQ=255)
                                              cGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATGGTAATAAACTGACCGTTGATTTTTAAGTCAACGCCGGCAGGAACAACGACCGGTGCGTTAGCAac                                                                                                     >  2:314382/1‑148 (MQ=255)
                                              cGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCCGCAGGAACAACGACCGGTGCTTTATCAac                                                                                                     >  2:3301129/1‑148 (MQ=255)
                                                aaGGTCAGGGTATTATCTCAATGTTTAACTTCAACAGCATCGTTGAGAGTACGCGTCAGCTCGCCGTTTTTACATTTTATCGTAATAACCTGACCGTTGATTTTAACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAacac                                                                                                   <  1:1761134/148‑1 (MQ=255)
                                                 aGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTGGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACg                                                                                                  <  2:3056469/148‑1 (MQ=255)
                                                    tCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTAAGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACgaga                                                                                               >  2:1101051/1‑148 (MQ=255)
                                                       gggTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACAt                                                                                            >  2:2103023/1‑148 (MQ=255)
                                                       gggTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACAt                                                                                            <  2:533810/148‑1 (MQ=255)
                                                        gttattatCTGCATGTTTAACTACAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTGGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACAtt                                                                                           <  2:3106181/146‑1 (MQ=255)
                                                        ggTATTATCTGCAGGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATAGTAATAAACAGACCCTTGATTTTTAAGTCAAAGCCGGCAGGAACAAACACCCGTGCTTTAGCAACACCAGACaat                                                                                           >  1:1034450/1‑146 (MQ=255)
                                                           attatCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTAAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACAttttt                                                                                        >  2:3449296/1‑148 (MQ=255)
                                                           attatCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGGCAACGCCGGCAGGAACAAAGACCCGTGCTTTAGCAACAAGCGACAttttt                                                                                        >  2:2427734/1‑148 (MQ=255)
                                                           attatCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAAAAACCTGACCGTTGATTTTTACGTAAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACAttttt                                                                                        >  2:3450123/1‑148 (MQ=255)
                                                             tatCTGCATTTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACATTTGATCGTAATAACCTGACCGTTTATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTtc                                                                                      <  1:2072265/148‑1 (MQ=255)
                                                              atCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTtcc                                                                                     <  2:3324277/148‑1 (MQ=255)
                                                                    ttGTTAAACTTCACCTGCTTCGTTGAGTGTACTTGTCAGCACGCCTTTTTTACCTTTTATCTTAAAAACCTGACCTTTTTTTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGAt                                                                               <  1:1274175/147‑1 (MQ=255)
                                                                      gTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTTATCGTAATAACCTGACCGTTTATTTTTACGTCAACGCCGGCTGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTa                                                                             <  2:1616475/148‑1 (MQ=255)
                                                                      gTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTa                                                                             <  2:1616487/148‑1 (MQ=255)
                                                                        ttAACTTCAACAGCATCTTTGAGATAACGAGTCCGCTCGCCTTTTTTACCTTTGAGCGAAATAACCTGACGGTTTATTTTTCCGCCACCGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAgg                                                                           <  1:3096532/148‑1 (MQ=255)
                                                                        ttAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAgg                                                                           >  2:3123309/1‑148 (MQ=255)
                                                                           aCTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTa                                                                        >  2:206832/1‑148 (MQ=255)
                                                                           aCTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAAACTGACCGTTGTTTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACAATTTTTCCTCCGATTAGGCTa                                                                        >  2:2991475/1‑148 (MQ=255)
                                                                              tCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCATTGATAGTAAAAACCAGACCCTTTATTTTTACGGCAACGCCCGCCGGAACAACCCCCGGTGCTTTAGCACCACGAGACAATTTTTACTCCGATTAGGCTACGt                                                                     >  1:503552/1‑148 (MQ=255)
                                                                                aaCAGCATCGTTGAGATTACGAGTCAGCTCGCCTTTTTTACCTTTGATCTAAATAACCTCACCTTTTATTTTTACGTCAACGCCGGCAGTAACCACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAg                                                                   <  1:1262121/148‑1 (MQ=255)
                                                                                     cATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACCACCGGTGCTTTATCAACACGAGACATTTTTTCCTCCGATTTGGCTACGTAGCAGAt                                                              >  2:1029697/1‑148 (MQ=255)
                                                                                       tCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATaa                                                            <  2:2034558/148‑1 (MQ=255)
                                                                                         gTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAAtt                                                          <  2:70389/148‑1 (MQ=255)
                                                                                             agagTACGAGTCAGCTCGCCGTTTTTACCTTTGAGCGTAATACCCTGACCGTTGTTTTTTCCGGCAACGCCGGCCGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGc                                                      <  1:3062416/148‑1 (MQ=255)
                                                                                                    gAGTCAGCTCGCTGTTTTTACTTTTGATCTTAATAACCTGACGGTTTTTTTTTACGTCAACGCGGGCAGTAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAg                                               <  1:1006496/148‑1 (MQ=255)
                                                                                                      gTCAGCTCGCGTTTTTTACCTTTGATCTTAATAACCTGACGGTTTATTTTTACGTCACCGCCGGCAGCAACCACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGAc                                             <  1:735532/148‑1 (MQ=255)
                                                                                                      gTCAGCTCGCCGTTTTTACCTTTGTTCGTAATAACCAGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCGTTAGCAACACGAGACATTTTTTCCTCCCGTTTGGCTACGTAGCAGATAATTTCGCCCCCAAGAc                                             >  1:3557715/1‑148 (MQ=255)
                                                                                                      gTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGAc                                             <  2:2103660/148‑1 (MQ=255)
                                                                                                       tCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGAcc                                            >  2:1872079/1‑148 (MQ=255)
                                                                                                         aGCTCGCCTTTTTTACCTTTGATCTTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGTAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAg                                          <  1:1461158/148‑1 (MQ=255)
                                                                                                         aGCTCGCCGTTTTTACCTTTGATCGTAATAACGTGACGGTTTATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAg                                          <  1:2027174/148‑1 (MQ=255)
                                                                                                         aGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTGCGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAg                                          <  1:3228067/148‑1 (MQ=255)
                                                                                                             cGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTg                                      <  2:3211116/148‑1 (MQ=255)
                                                                                                               ccGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCCGCAGGAAAAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGCTTAGGCTACGTAGCAGATAATTTAGCCACCAAGACCAGCCTGgc                                    >  2:1219171/1‑148 (MQ=255)
                                                                                                                 gTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGgcgc                                  >  1:2006192/1‑148 (MQ=255)
                                                                                                                  tttttACCTTTGATCGTAATAACCTGACCGTTGATTTTTCCGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGgcgcg                                 <  1:2888331/148‑1 (MQ=255)
                                                                                                                      tACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGc                             >  2:2122629/1‑148 (MQ=255)
                                                                                                                           ttGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGAt                        >  1:1286365/1‑148 (MQ=255)
                                                                                                                            ttaTCTTAATAACCTGCCGTTTTTTTTTTCCGTCAACGCCGGCAGCAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGATc                       <  1:2107460/146‑1 (MQ=255)
                                                                                                                            tGATCTTAATACCGTGGCCGTTTTTTTTAACGCCACCGCCGGCAGCAACCACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGATc                       <  1:315024/148‑1 (MQ=255)
                                                                                                                            tGATCGTAATAACCTGACCGTTTATTTTTACGTCAACGCCGGCAGGAAAAACGACCGGTGCTTTAGCAACACTAGACATTTTTTCCTCCTATTAGGCAACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGATc                       >  2:570528/1‑148 (MQ=255)
                                                                                                                            tGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGATc                       >  2:570834/1‑148 (MQ=255)
                                                                                                                            tGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGATc                       <  2:1975859/148‑1 (MQ=255)
                                                                                                                              aacgaaataaCCTGACCTTTTATTTTTACGACAACGCCGGCAGCAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGATCAg                     <  1:3283678/143‑1 (MQ=255)
                                                                                                                              aTCGTAATAACCTGACCTTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGATCAg                     <  1:2292962/148‑1 (MQ=255)
                                                                                                                              aTCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGATCAg                     >  2:2679692/1‑148 (MQ=255)
                                                                                                                                   aataaCCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGATCAGTCATa                <  1:2368761/148‑1 (MQ=255)
                                                                                                                                    ataaCCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGATCAGTCATaa               >  2:710994/1‑148 (MQ=255)
                                                                                                                                    ataaCCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGATCAGTCATaa               >  1:1008049/1‑148 (MQ=255)
                                                                                                                                    ataaCCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGATCAGTCATaa               >  1:49308/1‑148 (MQ=255)
                                                                                                                                    ataaCCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGATCAGTCATaa               >  2:2494207/1‑148 (MQ=255)
                                                                                                                                     taaCCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGATCAGTCATAac              >  1:972250/1‑148 (MQ=255)
                                                                                                                                     taaCCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGATCAGTCATAac              >  2:3310351/1‑148 (MQ=255)
                                                                                                                                     taaCCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTACTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGATCAGTCATaaa              >  1:113992/1‑147 (MQ=255)
                                                                                                                                        ccTGACCTTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGATCAGTCATAACAcc           <  2:3051152/148‑1 (MQ=255)
                                                                                                                                        ccTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGATCAGTCATAACAcc           <  2:739016/148‑1 (MQ=255)
                                                                                                                                          tGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGATCAGTCATAACACCtt         <  1:2611000/148‑1 (MQ=255)
                                                                                                                                           gACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGATCAGTCATAACACCttt        >  2:1150512/1‑148 (MQ=255)
                                                                                                                                            aCCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGATCAGTCATAACACCTTTa       >  1:2719033/1‑148 (MQ=255)
                                                                                                                                             ccGTTGATTTTTACGTCAACGCCGGCAGGAACCACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGATCAGTCATAACACCTTTag      <  1:2845914/148‑1 (MQ=255)
                                                                                                                                             ccGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGATCAGTCATAACACCTTTag      >  1:2540398/1‑148 (MQ=255)
                                                                                                                                              cGTTTATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGATCAGTCATAACACCTTTaga     <  1:342553/148‑1 (MQ=255)
                                                                                                                                              cGTTGATTTTTACGTCAACGCCGGCCGGAACCACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGATCAGTCATAACACCTTTaga     <  1:1845180/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GCGGTACCAGCCTGTGCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGATCAGTCATAACACCTTTAGAGGT  >  CP009273/3439297‑3439589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑