Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,440,444 G→T 100% S118S (TCC→TCA)  rplE ← 50S ribosomal subunit protein L5

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,440,4440GT100.0% 743.0 / NA 223S118S (TCC→TCA) rplE50S ribosomal subunit protein L5
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (115/108);  total (115/108)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 223 total aligned reads displayed.

GGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATTTGCG  >  CP009273/3440299‑3440587
                                                                                                                                                 |                                                                                                                                               
ggCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGa                                                                                                                                               <  2:540337/148‑1 (MQ=255)
 gCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGAc                                                                                                                                              <  1:2875772/148‑1 (MQ=255)
     tcttcGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGc                                                                                                                                          >  2:811171/1‑148 (MQ=255)
     tcttcGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGc                                                                                                                                          <  1:796781/148‑1 (MQ=255)
     tcttcGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGc                                                                                                                                          <  1:379566/148‑1 (MQ=255)
       ttcGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCa                                                                                                                                        <  1:2231167/148‑1 (MQ=255)
           tCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTCGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGa                                                                                                                                    <  2:1935740/148‑1 (MQ=255)
           tCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGa                                                                                                                                    <  2:1750399/148‑1 (MQ=255)
            cAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGaa                                                                                                                                   >  2:1562558/1‑148 (MQ=255)
              gATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGt                                                                                                                                 <  1:3555157/148‑1 (MQ=255)
              gATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGt                                                                                                                                 <  2:139391/148‑1 (MQ=255)
                  tCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACg                                                                                                                             >  1:347004/1‑148 (MQ=255)
                      agtagtGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATa                                                                                                                         <  2:1944317/148‑1 (MQ=255)
                       gtagtGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATAc                                                                                                                        <  2:755622/148‑1 (MQ=255)
                        tagtGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACg                                                                                                                       >  1:2380203/1‑148 (MQ=255)
                          gtGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAg                                                                                                                     <  2:496270/148‑1 (MQ=255)
                              taatggtaatATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTAc                                                                                                                 <  2:3540740/148‑1 (MQ=255)
                                  ggtaatATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCa                                                                                                             <  2:1445373/148‑1 (MQ=255)
                                  ggtaatATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCa                                                                                                             <  2:1027149/148‑1 (MQ=255)
                                     aatATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATa                                                                                                          <  2:2611140/148‑1 (MQ=255)
                                       tatCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGt                                                                                                        >  1:3435901/1‑148 (MQ=255)
                                          ccAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGAt                                                                                                     <  2:2093596/148‑1 (MQ=255)
                                          ccAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGAt                                                                                                     <  1:273488/148‑1 (MQ=255)
                                          ccAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGAt                                                                                                     <  1:2068741/148‑1 (MQ=255)
                                           cAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATc                                                                                                    >  2:3341303/1‑148 (MQ=255)
                                           cAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATc                                                                                                    >  2:3340951/1‑148 (MQ=255)
                                            aaaCCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCa                                                                                                   <  1:1218982/148‑1 (MQ=255)
                                            aaaCCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCa                                                                                                   >  1:2911482/1‑148 (MQ=255)
                                              aCCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAgg                                                                                                 <  2:1594345/148‑1 (MQ=255)
                                              aCCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAgg                                                                                                 <  2:3437820/148‑1 (MQ=255)
                                              aCCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAgg                                                                                                 >  2:19956/1‑148 (MQ=255)
                                              aCCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAgg                                                                                                 >  2:631376/1‑148 (MQ=255)
                                              aCCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAgg                                                                                                 >  1:1015598/1‑148 (MQ=255)
                                               ccACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGgc                                                                                                >  2:1021290/1‑148 (MQ=255)
                                               ccACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGgc                                                                                                >  1:2493200/1‑148 (MQ=255)
                                                 aCGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGCAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCACTAGTGATCAGgcgc                                                                                              >  2:1800814/1‑148 (MQ=255)
                                                 aCGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACTACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGgcgc                                                                                              >  1:2521009/1‑148 (MQ=255)
                                                  cGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCt                                                                                             <  1:1737718/148‑1 (MQ=255)
                                                  cGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCt                                                                                             >  1:695682/1‑148 (MQ=255)
                                                  cGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCt                                                                                             <  1:278306/148‑1 (MQ=255)
                                                      cgcgGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCaaa                                                                                         >  2:115401/1‑148 (MQ=255)
                                                         ggTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGaa                                                                                      <  2:2196910/148‑1 (MQ=255)
                                                             gACTTTATCGTAGTCGTTTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTcc                                                                                  <  1:1578216/148‑1 (MQ=255)
                                                                 ttATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCcaca                                                                              <  1:2128154/148‑1 (MQ=255)
                                                                   aTCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATg                                                                            <  1:3537604/148‑1 (MQ=255)
                                                                    tCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGc                                                                           >  2:1363619/1‑148 (MQ=255)
                                                                     cGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCg                                                                          <  2:1084198/148‑1 (MQ=255)
                                                                      gTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGt                                                                         <  2:3393608/148‑1 (MQ=255)
                                                                       tAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGtt                                                                        >  2:2806224/1‑148 (MQ=255)
                                                                             aTTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCAc                                                                  >  2:2282224/1‑148 (MQ=255)
                                                                               ttCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGACCTCCCACATGCGTTCGCCACGc                                                                >  1:429834/1‑148 (MQ=255)
                                                                               ttCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGc                                                                >  2:2675348/1‑148 (MQ=255)
                                                                               ttCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGc                                                                >  2:895461/1‑148 (MQ=255)
                                                                               ttCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGc                                                                >  1:2258275/1‑148 (MQ=255)
                                                                                  tGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACNGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCaga                                                             <  1:1802834/148‑1 (MQ=255)
                                                                                  tGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCaga                                                             <  1:2847186/148‑1 (MQ=255)
                                                                                   gggAAGATGATCTGCTCACTGACACCCATGCTGTAGTTACCACGACAGTCGAAAGACTTAGCTGACAGGCCACTGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCagag                                                            <  2:1826039/148‑1 (MQ=255)
                                                                                   gggAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCagag                                                            >  2:3321443/1‑148 (MQ=255)
                                                                                    ggAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCAAATGCGTTCGCCACGCAGAGt                                                           <  1:1022373/148‑1 (MQ=255)
                                                                                     gAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGACCTCCCACATGCGTTCGCCACGCAGAGtt                                                          >  2:1275719/1‑148 (MQ=255)
                                                                                     gAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGtt                                                          >  2:3222651/1‑148 (MQ=255)
                                                                                      aaGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTCCAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTa                                                         >  1:2142332/1‑148 (MQ=255)
                                                                                        gatgatCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACt                                                       >  1:1173122/1‑148 (MQ=255)
                                                                                         atgatCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACtt                                                      <  1:966358/148‑1 (MQ=255)
                                                                                           gatCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTa                                                    <  2:1284480/148‑1 (MQ=255)
                                                                                           gatCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTa                                                    >  1:2533439/1‑148 (MQ=255)
                                                                                           gatCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTa                                                    >  2:2504558/1‑148 (MQ=255)
                                                                                             tCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACa                                                  >  1:2801766/1‑148 (MQ=255)
                                                                                               tGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGc                                                <  1:1051975/148‑1 (MQ=255)
                                                                                               tGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGc                                                <  2:313502/148‑1 (MQ=255)
                                                                                               tGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGc                                                >  1:767671/1‑148 (MQ=255)
                                                                                                  tCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGa                                             <  1:424760/148‑1 (MQ=255)
                                                                                                  tCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGa                                             <  1:423668/148‑1 (MQ=255)
                                                                                                        acacCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGAt                                       >  1:414287/1‑148 (MQ=255)
                                                                                                        acacCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGAt                                       >  1:3538384/1‑148 (MQ=255)
                                                                                                        acacCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGAt                                       >  2:2430734/1‑148 (MQ=255)
                                                                                                         cacCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATa                                      >  1:2963077/1‑148 (MQ=255)
                                                                                                            ccATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGcc                                   <  1:2849562/148‑1 (MQ=255)
                                                                                                               tGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTg                                >  1:2442092/1‑148 (MQ=255)
                                                                                                               tGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTg                                <  1:3295614/148‑1 (MQ=255)
                                                                                                               tGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTg                                <  2:2747663/148‑1 (MQ=255)
                                                                                                                gCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACCTGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGa                               >  1:662172/1‑148 (MQ=255)
                                                                                                                   gTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGTCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACgg                            >  2:2471336/1‑148 (MQ=255)
                                                                                                                    tAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGa                           >  2:2325271/1‑148 (MQ=255)
                                                                                                                     aGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGAt                          <  2:3088065/148‑1 (MQ=255)
                                                                                                                      gTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGAtt                         <  1:575800/148‑1 (MQ=255)
                                                                                                                        tACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGAtttt                       >  2:435270/1‑148 (MQ=255)
                                                                                                                           cACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGaa                    <  1:3029335/148‑1 (MQ=255)
                                                                                                                           cACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGaa                    >  1:1895358/1‑148 (MQ=255)
                                                                                                                              gACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGcc                 >  2:235403/1‑148 (MQ=255)
                                                                                                                               aCCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCt                >  1:485848/1‑148 (MQ=255)
                                                                                                                               aCCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCt                >  1:3259580/1‑148 (MQ=255)
                                                                                                                                    cGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAAc           >  1:1149782/1‑148 (MQ=255)
                                                                                                                                      aaaGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAg         >  1:3132676/1‑148 (MQ=255)
                                                                                                                                      aaaGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAg         <  2:2009523/148‑1 (MQ=255)
                                                                                                                                      aaaGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAg         <  1:2249223/148‑1 (MQ=255)
                                                                                                                                        aGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGAt       <  2:3270146/148‑1 (MQ=255)
                                                                                                                                        aGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGAt       <  1:2282224/148‑1 (MQ=255)
                                                                                                                                         gACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGAtt      <  2:2155347/148‑1 (MQ=255)
                                                                                                                                         gACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGAtt      >  1:548586/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
GGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATTTGCG  >  CP009273/3440299‑3440587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑