Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,455,867 C→A 100% I55I (ATC→ATA)  gspJ → putative general secretory pathway component, cryptic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,455,8670CA99.5% 592.9 / ‑4.2 181I55I (ATC→ATA) gspJputative general secretory pathway component, cryptic
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (83/97);  total (83/98)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 189 total aligned reads displayed.

TTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTC  >  CP009273/3455724‑3456011
                                                                                                                                               |                                                                                                                                                
ttCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGcaccataacca                                                                                                                                              <  2:206307/148‑1 (MQ=255)
ttCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGcaccataacca                                                                                                                                              <  1:2487484/148‑1 (MQ=255)
  cacaTTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCAtt                                                                                                                                            <  1:3397679/148‑1 (MQ=255)
   acaTTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTc                                                                                                                                           <  1:1567088/148‑1 (MQ=255)
    caTTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCt                                                                                                                                          <  1:479583/148‑1 (MQ=255)
     aTTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCtt                                                                                                                                         <  2:845359/148‑1 (MQ=255)
       tGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTTTGATTTTTTAGCAAGCTTGCCAGTTGCATCAACGCTAACAGAAAGAAAAACAGCAGTTAAATAAGCTACACCGCACCATAAACAATCTTGa                                                                                                                                       >  1:3494383/1‑148 (MQ=255)
        gCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGAt                                                                                                                                      <  2:2673205/148‑1 (MQ=255)
            gAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACg                                                                                                                                  <  2:3466179/148‑1 (MQ=255)
            gAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACg                                                                                                                                  <  1:2424746/148‑1 (MQ=255)
             aGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGa                                                                                                                                 <  2:2797999/148‑1 (MQ=255)
               gTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATTATTTTTTCGCAAGCGTGCGAGTTTGAACAACGCTCACACAAAGAAATACAGCACTTCAATCAGCGACACCGCACCAAAACCAATCTTGATAACGAtc                                                                                                                               >  1:2895020/1‑148 (MQ=255)
                tCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATTTTTTTTTGGCAAGCTTACGCGTTGCATCAACGCGCACAGAAAGAAATCCAGCCGTTCAAACAGCTACAGCGCCCCATAACCCTTCTTGATAAAGAtct                                                                                                                              >  2:1259547/1‑148 (MQ=255)
                 cATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGAtctc                                                                                                                             <  1:2496206/148‑1 (MQ=255)
                    ggcggcGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCCGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTg                                                                                                                          >  2:1126610/1‑148 (MQ=255)
                        gcgcTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAAc                                                                                                                      <  1:248696/148‑1 (MQ=255)
                        gcgcTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAAc                                                                                                                      <  1:2042909/148‑1 (MQ=255)
                           cTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTgg                                                                                                                   <  2:744818/148‑1 (MQ=255)
                            tGGCGATTTTCTCACTGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGt                                                                                                                  <  2:1591239/148‑1 (MQ=255)
                               cGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGc                                                                                                               <  1:2796822/148‑1 (MQ=255)
                                gATTTTCTCAATTCTTAGCGTGCTGGCGTTTATGATTTTTTCTCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCAACATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGcc                                                                                                              <  2:3495315/148‑1 (MQ=255)
                                gATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGcc                                                                                                              <  2:104292/148‑1 (MQ=255)
                                gATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGcc                                                                                                              <  2:47530/148‑1 (MQ=255)
                                  ttttCTCAATGCTTAGCGTGCTGGCGTTTATTATTTTTTAGCAAGCTTCCGAGTTTCATCAACGCTCACAGAAAGAAAAACAGCAGTTCAATCAGCTACAGCGCACCATAAACATTATTGATAACGATATCCAGCAAAAGGTCGCCCg                                                                                                            >  1:816488/1‑148 (MQ=255)
                                  ttttCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTAGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTAAATCAGCTACAGCGCACCATAACCATTCTTGATAAAGATCTACTGCAACAGGTCGCCCg                                                                                                            >  2:1634875/1‑148 (MQ=255)
                                   tttCTCACTGCTTAGCGTGCTGGCGTTTATGGTTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCgg                                                                                                           <  2:3399917/148‑1 (MQ=255)
                                   tttCTCAATGCTTAGCGTGCTGGCGTTTATTATTTTTTAGCAAGCTTACGAGTTGCAACAACGCTCACAGAAAGAAATCCAGCAGTTAAATCATCTACAGCGCACCAAAACCAAACATGATAAACATCTCCTGCAAAAGGTAGccccg                                                                                                           >  1:3203328/1‑146 (MQ=255)
                                     tctcAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCggag                                                                                                         <  1:2924914/148‑1 (MQ=255)
                                     tctcAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCggag                                                                                                         <  1:2924935/148‑1 (MQ=255)
                                        cAATGCTTAGCGTGCTGGCGTTTATTATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATACAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGaa                                                                                                      >  2:3060782/1‑148 (MQ=255)
                                         aaTGCTTAGCGTGCTGGCGTTTATGATTTTTTAGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAAt                                                                                                     >  2:3215568/1‑148 (MQ=255)
                                             cTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGa                                                                                                 <  1:3396588/148‑1 (MQ=255)
                                             cTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGa                                                                                                 <  1:819850/148‑1 (MQ=255)
                                             cTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAAACATTCTTGATAACGATCTCCTGCAAATGGTCGCCCGGAGGAATCGGa                                                                                                 >  2:2702876/1‑148 (MQ=255)
                                              ttAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAg                                                                                                <  1:2109137/148‑1 (MQ=255)
                                              ttAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAg                                                                                                <  1:3552973/148‑1 (MQ=255)
                                                aGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTa                                                                                              <  1:222362/148‑1 (MQ=255)
                                                       tGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACaa                                                                                       <  2:1184655/148‑1 (MQ=255)
                                                        ggCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACaaa                                                                                      >  2:3474825/1‑148 (MQ=255)
                                                         gCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACaaaa                                                                                     >  2:336904/1‑148 (MQ=255)
                                                         gCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACaaaa                                                                                     >  2:2960900/1‑148 (MQ=255)
                                                         gCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACaaaa                                                                                     >  2:776030/1‑148 (MQ=255)
                                                              tATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACCAAATAATg                                                                                >  2:2075657/1‑148 (MQ=255)
                                                               aTGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATgg                                                                               >  1:1376851/1‑148 (MQ=255)
                                                               aTGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATgg                                                                               <  2:235037/148‑1 (MQ=255)
                                                                 gATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGgtg                                                                             <  2:939847/148‑1 (MQ=255)
                                                                 gATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGgtg                                                                             >  1:2989311/1‑148 (MQ=255)
                                                                 gATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGgtg                                                                             >  1:2103179/1‑148 (MQ=255)
                                                                 gATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGgtg                                                                             >  1:2709187/1‑148 (MQ=255)
                                                                   ttttttCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGtt                                                                           <  1:1923301/148‑1 (MQ=255)
                                                                     ttttCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTgg                                                                         <  2:1177587/148‑1 (MQ=255)
                                                                      tttCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTggg                                                                        <  2:1853356/148‑1 (MQ=255)
                                                                         cGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTga                                                                     >  2:686782/1‑148 (MQ=255)
                                                                            aaGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTgaaga                                                                  <  2:3482499/148‑1 (MQ=255)
                                                                              gCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGaagaag                                                                <  2:2503157/148‑1 (MQ=255)
                                                                              gCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGaagaag                                                                <  1:3218037/148‑1 (MQ=255)
                                                                               cTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGc                                                               <  1:2923004/148‑1 (MQ=255)
                                                                                ttCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCt                                                              >  2:2912576/1‑148 (MQ=255)
                                                                                 tCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTa                                                             <  1:2313219/148‑1 (MQ=255)
                                                                                 tCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTa                                                             >  1:1245323/1‑148 (MQ=255)
                                                                                 tCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTa                                                             >  2:2026182/1‑148 (MQ=255)
                                                                                  ccGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTATAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTAt                                                            >  1:3473264/1‑148 (MQ=255)
                                                                                  ccGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATATCGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATTATGGTGGTGGGTGAAGAAGCTAt                                                            <  2:2685001/148‑1 (MQ=255)
                                                                                  ccGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTAt                                                            <  1:870979/148‑1 (MQ=255)
                                                                                   cGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTAtt                                                           >  2:3080531/1‑148 (MQ=255)
                                                                                    gAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTAttt                                                          <  2:230315/148‑1 (MQ=255)
                                                                                     aGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTAtttt                                                         <  2:3316442/148‑1 (MQ=255)
                                                                                     aGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTAtttt                                                         >  2:140870/1‑148 (MQ=255)
                                                                                       ttGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTa                                                       <  1:580513/148‑1 (MQ=255)
                                                                                       ttGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTa                                                       <  1:581284/148‑1 (MQ=255)
                                                                                         gCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTAcc                                                     >  1:1108830/1‑148 (MQ=255)
                                                                                                cGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCaaa                                              >  1:6835/1‑148 (MQ=255)
                                                                                                cGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCaaa                                              <  1:1260137/148‑1 (MQ=255)
                                                                                                 gCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAg                                             <  1:3255689/148‑1 (MQ=255)
                                                                                                  cTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGt                                            <  2:2526795/148‑1 (MQ=255)
                                                                                                         aaagaaaTCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATc                                     >  1:908273/1‑148 (MQ=255)
                                                                                                          aagaaaTCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACTCAGAGTCGCGATcc                                    >  1:1036171/1‑148 (MQ=255)
                                                                                                           agaaaTCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCg                                   <  1:438258/148‑1 (MQ=255)
                                                                                                             aaaTCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCt                                 >  1:3204475/1‑148 (MQ=255)
                                                                                                               aTCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTgg                               >  1:2360874/1‑148 (MQ=255)
                                                                                                                tCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGc                              >  1:2563425/1‑148 (MQ=255)
                                                                                                                tCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGc                              <  2:2830947/148‑1 (MQ=255)
                                                                                                                tCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGc                              >  1:523934/1‑148 (MQ=255)
                                                                                                                   agcagTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGctcc                           <  2:186802/148‑1 (MQ=255)
                                                                                                                   agcagTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGctcc                           <  2:185924/148‑1 (MQ=255)
                                                                                                                        ttCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCa                      >  2:1788543/1‑148 (MQ=255)
                                                                                                                          cAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGt                    <  1:1867464/148‑1 (MQ=255)
                                                                                                                           aaTCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTg                   <  1:3079650/148‑1 (MQ=255)
                                                                                                                             tCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGaa                 >  2:2759826/1‑148 (MQ=255)
                                                                                                                             tCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGaa                 <  2:2601935/148‑1 (MQ=255)
                                                                                                                              cAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGATGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCACAGTCGCGATCCGCTGGCTCCTCTCAGTGAAg                >  1:1084984/1‑148 (MQ=255)
                                                                                                                              cAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAg                >  2:1506026/1‑148 (MQ=255)
                                                                                                                                gCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGca              <  1:3131807/148‑1 (MQ=255)
                                                                                                                                 cTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGcac             <  1:1778481/148‑1 (MQ=255)
                                                                                                                                   aCAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACaa           <  2:2820624/148‑1 (MQ=255)
                                                                                                                                     aGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAAc         >  2:2216870/1‑148 (MQ=255)
                                                                                                                                     aGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAAc         <  1:2616860/148‑1 (MQ=255)
                                                                                                                                      gcgcACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAAcc        <  2:1593630/148‑1 (MQ=255)
                                                                                                                                         caccataaccatTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTa     <  1:3528609/148‑1 (MQ=255)
                                                                                                                                            cataaccatTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTc  >  1:1909029/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                                
TTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTC  >  CP009273/3455724‑3456011

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑