Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,470,272 C→A 92.5% V169V (GTG→GTT)  fkpA ← FKBP‑type peptidyl‑prolyl cis‑trans isomerase (rotamase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,470,2720CA92.5% 607.4 / 37.2 221V169V (GTG→GTT) fkpAFKBP‑type peptidyl‑prolyl cis‑trans isomerase (rotamase)
Reads supporting (aligned to +/- strand):  ref base C (11/6);  new base A (103/101);  total (114/107)
Fisher's exact test for biased strand distribution p-value = 3.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 221 total aligned reads displayed.

CAGTTTGATCTTACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTCACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCTTCGTTATCAGCCG  >  CP009273/3470128‑3470417
                                                                                                                                                |                                                                                                                                                 
cAGTTTGATCTTACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACt                                                                                                                                                >  1:1482653/1‑148 (MQ=255)
  gTTTGATCTTACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTCACTac                                                                                                                                              >  2:877381/1‑148 (MQ=255)
    ttGATCTTACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTacaa                                                                                                                                            >  2:363981/1‑148 (MQ=255)
    ttGATCTTACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTacaa                                                                                                                                            <  1:1420960/148‑1 (MQ=255)
    ttGATCTTACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTAACCGTCGATCAGCGTACCTTTGTAGTTAACTacaa                                                                                                                                            >  1:3372673/1‑148 (MQ=255)
     tGATCTTACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTacaac                                                                                                                                           <  2:2221418/148‑1 (MQ=255)
     tGATCTTACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTacaac                                                                                                                                           <  2:2221826/148‑1 (MQ=255)
        tCTTACCGCGTTTCTTGATGATCTTAAGACCTTCTGTACAACACGGGATAACACCGTCCAGACGGAAAGAAAGCGTTGCACCACGGGTGTACGAGTTGTCGAACAATATACCGTCGATCAGCGAACCTTTGTATTTAAATACAAAAGt                                                                                                                                        >  2:1923934/1‑148 (MQ=255)
        tCTTACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATACCACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGt                                                                                                                                        <  2:2414718/148‑1 (MQ=255)
        tCTTACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTCACTacaacact                                                                                                                                        >  1:1635989/1‑146 (MQ=255)
        tCTTACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTCACTACAACAGt                                                                                                                                        >  1:3394884/1‑148 (MQ=255)
        tCTTACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGt                                                                                                                                        <  1:1164193/148‑1 (MQ=255)
         cTTACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTa                                                                                                                                       >  1:157432/1‑148 (MQ=255)
         cTTACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTa                                                                                                                                       >  1:1787700/1‑148 (MQ=255)
          ttACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTAt                                                                                                                                      <  1:2552181/148‑1 (MQ=255)
          ttACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTAt                                                                                                                                      <  1:2516587/148‑1 (MQ=255)
            aCCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCg                                                                                                                                    <  1:1423808/148‑1 (MQ=255)
            aCCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCg                                                                                                                                    <  1:2374821/148‑1 (MQ=255)
                ccTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATAGCTGt                                                                                                                                >  2:248458/1‑148 (MQ=255)
                  tttCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCt                                                                                                                              <  2:3179785/148‑1 (MQ=255)
                  tttCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCt                                                                                                                              >  2:183234/1‑148 (MQ=255)
                      ttGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCg                                                                                                                          <  1:2673009/148‑1 (MQ=255)
                       tGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCgg                                                                                                                         >  1:1057318/1‑148 (MQ=255)
                          tGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGc                                                                                                                      >  2:2890212/1‑148 (MQ=255)
                           gTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCt                                                                                                                     >  1:3515999/1‑148 (MQ=255)
                            ttcttcAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCtt                                                                                                                    <  1:2831358/148‑1 (MQ=255)
                            ttcttcAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTGTCGGTGCtt                                                                                                                    >  1:3359105/1‑148 (MQ=255)
                             tcttcAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTc                                                                                                                   >  2:3156807/1‑148 (MQ=255)
                             tcttcAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTc                                                                                                                   >  1:356058/1‑148 (MQ=255)
                              cttcAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTAGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCg                                                                                                                  >  2:3100404/1‑148 (MQ=255)
                               ttcAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGc                                                                                                                 <  1:1432348/148‑1 (MQ=255)
                                tcAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGcc                                                                                                                >  2:2693351/1‑148 (MQ=255)
                                 cAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCt                                                                                                               <  1:13201/148‑1 (MQ=255)
                                   gACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCttt                                                                                                             >  1:1546110/1‑148 (MQ=255)
                                      cTTGTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGTTACAACACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTAAAAATGTTGTTAACTAAAACAGTATCGCTGTCGTTCGGTGCTTAGCCTTTAca                                                                                                          >  2:2942325/1‑147 (MQ=255)
                                      cTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTCACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTAcc                                                                                                          >  1:2879118/1‑148 (MQ=255)
                                      cTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTAcc                                                                                                          >  2:2942304/1‑148 (MQ=255)
                                         cTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGc                                                                                                       <  1:1955901/148‑1 (MQ=255)
                                           gTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCtt                                                                                                     >  1:1224482/1‑148 (MQ=255)
                                           gTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCtt                                                                                                     <  2:2538765/148‑1 (MQ=255)
                                               aaCCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTctac                                                                                                 <  1:2796608/148‑1 (MQ=255)
                                                   cGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTAcc                                                                                             <  2:347585/148‑1 (MQ=255)
                                                    gggATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCt                                                                                            <  1:2935262/148‑1 (MQ=255)
                                                      gATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGa                                                                                          >  2:3375023/1‑148 (MQ=255)
                                                       aTAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTCACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGAt                                                                                         <  2:1052695/148‑1 (MQ=255)
                                                          acacCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATaaa                                                                                      <  1:66252/148‑1 (MQ=255)
                                                              cGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAaccag                                                                                  >  2:1711482/1‑148 (MQ=255)
                                                               gTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTCACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAaccaga                                                                                 <  1:877381/148‑1 (MQ=255)
                                                                   aGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTCACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAaccagaccag                                                                             >  1:2457499/1‑148 (MQ=255)
                                                                   aGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCGTTACCGGCTTCTACTACCTGATAAaccagaccag                                                                             >  2:2862660/1‑148 (MQ=255)
                                                                    gACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGt                                                                            <  2:1138743/148‑1 (MQ=255)
                                                                      cGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTg                                                                          >  2:2166427/1‑148 (MQ=255)
                                                                       ggAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGa                                                                         <  1:2313108/148‑1 (MQ=255)
                                                                       ggAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGa                                                                         <  1:948812/148‑1 (MQ=255)
                                                                           agaaagCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTAGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAagag                                                                     >  2:2839265/1‑148 (MQ=255)
                                                                             aaagCTGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCCGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAAACAGACCAGTTGAAGAggg                                                                   >  1:160791/1‑147 (MQ=255)
                                                                             aaagCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGt                                                                   <  2:2316574/148‑1 (MQ=255)
                                                                                    ttCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTcaca                                                            <  1:920810/148‑1 (MQ=255)
                                                                                           cGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTtct                                                     >  2:3253846/1‑148 (MQ=255)
                                                                                           cGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAACGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTtct                                                     <  1:1465844/148‑1 (MQ=255)
                                                                                             ggTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTctct                                                   <  1:2767909/148‑1 (MQ=255)
                                                                                               tgtAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTTATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCttt                                                 <  1:2042312/148‑1 (MQ=255)
                                                                                                gtAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTg                                                >  1:843691/1‑148 (MQ=255)
                                                                                                     agTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACCCCTTTCTCTTTGGCaaa                                           >  2:431639/1‑148 (MQ=255)
                                                                                                     agTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCaaa                                           <  2:1637792/148‑1 (MQ=255)
                                                                                                     agTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCaaa                                           <  2:2632995/148‑1 (MQ=255)
                                                                                                      gTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAAt                                          >  2:3280289/1‑148 (MQ=255)
                                                                                                       ttGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTCACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTATCTTTGGCAAAtt                                         >  1:1924779/1‑148 (MQ=255)
                                                                                                       ttGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAAtt                                         >  1:2582077/1‑148 (MQ=255)
                                                                                                         gTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTtc                                       <  2:3451338/148‑1 (MQ=255)
                                                                                                            gAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTcg                                    <  1:1819092/148‑1 (MQ=255)
                                                                                                            gAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTcg                                    <  1:2103654/148‑1 (MQ=255)
                                                                                                            gAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTcg                                    <  1:3062555/148‑1 (MQ=255)
                                                                                                              aCTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTcgcg                                  >  1:796681/1‑148 (MQ=255)
                                                                                                                tctTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGt                                >  2:2776083/1‑148 (MQ=255)
                                                                                                                 ctTTACCGTCGATCAGCGTACCTTTGTAGTTCACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGATCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTa                               >  2:739197/1‑148 (MQ=255)
                                                                                                                 ctTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGACTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTa                               <  1:545377/148‑1 (MQ=255)
                                                                                                                 ctTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGACTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTa                               <  1:545464/148‑1 (MQ=255)
                                                                                                                 ctTTACCGTAGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTa                               <  1:3100404/148‑1 (MQ=255)
                                                                                                                      ccGTAGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCtt                          <  2:1528741/148‑1 (MQ=255)
                                                                                                                         tCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTAc                       >  1:1272250/1‑148 (MQ=255)
                                                                                                                         tCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTAc                       <  1:2266577/148‑1 (MQ=255)
                                                                                                                         tCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTATACCGGCTTCTACTACCTGATAAACCAGACCACTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTAc                       >  1:2168389/1‑148 (MQ=255)
                                                                                                                          cGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTAcc                      >  1:470985/1‑148 (MQ=255)
                                                                                                                           gATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCt                     <  2:843691/148‑1 (MQ=255)
                                                                                                                            aTCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCtt                    >  1:1006257/1‑148 (MQ=255)
                                                                                                                            aTCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCtt                    >  2:427159/1‑148 (MQ=255)
                                                                                                                             tCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCttt                   <  1:2703046/148‑1 (MQ=255)
                                                                                                                               aGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCTGTACTCTTTACCTTTTg                 <  2:3372673/148‑1 (MQ=255)
                                                                                                                               aGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTg                 <  2:1384652/148‑1 (MQ=255)
                                                                                                                               aGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTg                 >  2:2363679/1‑148 (MQ=255)
                                                                                                                                 cGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCt               >  2:1064879/1‑148 (MQ=255)
                                                                                                                                 cGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCt               >  2:68439/1‑148 (MQ=255)
                                                                                                                                  gTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCtt              <  2:368496/148‑1 (MQ=255)
                                                                                                                                    aCCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCTTCg            <  1:427159/148‑1 (MQ=255)
                                                                                                                                    aCCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCTTCg            <  1:3093980/148‑1 (MQ=255)
                                                                                                                                    aCCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCTTCg            >  2:2039553/1‑148 (MQ=255)
                                                                                                                                      cTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCTTCGtt          >  1:821053/1‑148 (MQ=255)
                                                                                                                                      cTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCTTCGtt          >  2:1235083/1‑148 (MQ=255)
                                                                                                                                        ttGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCTTCGTTAt        <  1:699896/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
CAGTTTGATCTTACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTCACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCTTCGTTATCAGCCG  >  CP009273/3470128‑3470417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑