Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,479,769 A→T 100% E97D (GAA→GAT)  crp → cAMP‑activated global transcription factor, mediator of catabolite repression

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,479,7690AT100.0% 658.2 / NA 199E97D (GAA→GAT) crpcAMP‑activated global transcription factor, mediator of catabolite repression
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (106/93);  total (106/93)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 206 total aligned reads displayed.

CAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCA  >  CP009273/3479624‑3479913
                                                                                                                                                 |                                                                                                                                                
cAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGatat                                                                                                                                                >  2:1928063/1‑148 (MQ=255)
cAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGatat                                                                                                                                                <  2:2038173/148‑1 (MQ=255)
 aGTGCTTATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATAtt                                                                                                                                               <  1:3266626/148‑1 (MQ=255)
   tGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTc                                                                                                                                             >  1:507174/1‑148 (MQ=255)
       gATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTAc                                                                                                                                         >  2:2777893/1‑148 (MQ=255)
         tCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACaa                                                                                                                                       >  1:1756771/1‑148 (MQ=255)
          cAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACaaa                                                                                                                                      >  2:2306071/1‑148 (MQ=255)
           aaaGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACaaaa                                                                                                                                     <  1:1790058/148‑1 (MQ=255)
           aaaGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACaaaa                                                                                                                                     <  1:381171/148‑1 (MQ=255)
                cGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAAttt                                                                                                                                >  2:1192658/1‑148 (MQ=255)
                  aaGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCg                                                                                                                              >  1:3456302/1‑148 (MQ=255)
                   agagGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGc                                                                                                                             >  2:1771360/1‑148 (MQ=255)
                    gagGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGCGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGcc                                                                                                                            >  1:359144/1‑148 (MQ=255)
                     agGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCa                                                                                                                           <  2:891776/148‑1 (MQ=255)
                      gggTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCaa                                                                                                                          <  2:2192244/148‑1 (MQ=255)
                      gggTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCaa                                                                                                                          <  1:1263818/148‑1 (MQ=255)
                       ggTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAAt                                                                                                                         >  1:2853920/1‑148 (MQ=255)
                         tAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCACTTg                                                                                                                       >  2:3283477/1‑148 (MQ=255)
                         tAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTg                                                                                                                       <  2:746885/148‑1 (MQ=255)
                           aaGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGAt                                                                                                                     >  1:86143/1‑148 (MQ=255)
                           aaGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGAt                                                                                                                     >  1:27485/1‑148 (MQ=255)
                             gAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTc                                                                                                                   <  2:2696308/148‑1 (MQ=255)
                              aaaTGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCa                                                                                                                  >  1:2979289/1‑148 (MQ=255)
                                aTGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAgg                                                                                                                >  2:2299915/1‑148 (MQ=255)
                                 tGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGt                                                                                                               >  1:1240965/1‑148 (MQ=255)
                                  gATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTa                                                                                                              >  2:1322876/1‑148 (MQ=255)
                                    tCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTaaa                                                                                                            <  2:1250790/148‑1 (MQ=255)
                                    tCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTaaa                                                                                                            <  1:2092514/148‑1 (MQ=255)
                                    tCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTaaa                                                                                                            <  2:2927319/148‑1 (MQ=255)
                                     ccTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAAc                                                                                                           <  1:1058924/148‑1 (MQ=255)
                                        ctcCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCg                                                                                                        >  2:1823190/1‑148 (MQ=255)
                                        ctcCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCg                                                                                                        >  2:3382561/1‑148 (MQ=255)
                                            tATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACa                                                                                                    >  1:1577972/1‑148 (MQ=255)
                                            tATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACa                                                                                                    <  1:1771360/148‑1 (MQ=255)
                                            tATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACa                                                                                                    <  2:2128367/148‑1 (MQ=255)
                                             aTCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACAt                                                                                                   >  1:702610/1‑148 (MQ=255)
                                              tCTGAATCAGTGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACAtt                                                                                                  <  1:2199082/148‑1 (MQ=255)
                                               cTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATCTCGCCAATTGATTCAGGTAAACCCGGACATTc                                                                                                 >  2:3002923/1‑148 (MQ=255)
                                                  aaTCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGa                                                                                              >  2:2671991/1‑148 (MQ=255)
                                                  aaTCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGa                                                                                              >  2:1927010/1‑148 (MQ=255)
                                                     cAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGc                                                                                           >  2:1723269/1‑148 (MQ=255)
                                                      aGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCg                                                                                          <  2:1892963/148‑1 (MQ=255)
                                                       gggTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGt                                                                                         <  2:867812/148‑1 (MQ=255)
                                                       gggTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGt                                                                                         >  2:3504468/1‑148 (MQ=255)
                                                        ggTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGtt                                                                                        <  2:842971/148‑1 (MQ=255)
                                                        ggTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGtt                                                                                        <  2:3180994/148‑1 (MQ=255)
                                                        ggTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGtt                                                                                        <  1:1928063/148‑1 (MQ=255)
                                                        ggTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGtt                                                                                        >  2:1980939/1‑148 (MQ=255)
                                                        ggTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGtt                                                                                        >  2:185309/1‑148 (MQ=255)
                                                           gATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGt                                                                                     <  1:3308205/148‑1 (MQ=255)
                                                           gATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGt                                                                                     >  2:2582416/1‑148 (MQ=255)
                                                            aTTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTc                                                                                    <  2:1179470/148‑1 (MQ=255)
                                                               ttATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGc                                                                                 <  2:246890/148‑1 (MQ=255)
                                                                   tGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAg                                                                             <  1:3053736/148‑1 (MQ=255)
                                                                   tGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAg                                                                             <  1:3123531/148‑1 (MQ=255)
                                                                   tGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAg                                                                             <  2:1663937/148‑1 (MQ=255)
                                                                   tGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAg                                                                             >  2:1626163/1‑148 (MQ=255)
                                                                     gCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGAt                                                                           <  1:219044/148‑1 (MQ=255)
                                                                         aCTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGgcg                                                                       >  1:1410854/1‑148 (MQ=255)
                                                                            gggCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGcgt                                                                    >  2:2655713/1‑148 (MQ=255)
                                                                               ccTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGcgtcgt                                                                 >  2:3138946/1‑148 (MQ=255)
                                                                                  gTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTg                                                              >  2:3046939/1‑148 (MQ=255)
                                                                                   tttGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGc                                                             >  1:942269/1‑148 (MQ=255)
                                                                                   tttGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGc                                                             <  2:3305727/148‑1 (MQ=255)
                                                                                       aaGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGt                                                         <  2:3076102/148‑1 (MQ=255)
                                                                                          agGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCAc                                                      >  1:690637/1‑148 (MQ=255)
                                                                                            ggCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACtt                                                    >  2:3472558/1‑148 (MQ=255)
                                                                                                     cGTAGCGCATGGGTACGTGCGAAAACCTCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCTTCGTCTGCAAGTCACTTCAGAGAAAg                                           <  1:913227/148‑1 (MQ=255)
                                                                                                     cGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAg                                           >  1:891603/1‑148 (MQ=255)
                                                                                                      gTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGt                                          <  1:738582/148‑1 (MQ=255)
                                                                                                         gcgcATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTggg                                       <  2:1708968/148‑1 (MQ=255)
                                                                                                          cgcATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGc                                      <  2:117641/148‑1 (MQ=255)
                                                                                                          cgcATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGc                                      >  2:721260/1‑148 (MQ=255)
                                                                                                            cATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCaa                                    <  1:679461/148‑1 (MQ=255)
                                                                                                             aTGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAAc                                   <  2:1456372/148‑1 (MQ=255)
                                                                                                             aTGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAAc                                   >  2:1271234/1‑148 (MQ=255)
                                                                                                                ggTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTg                                >  1:693529/1‑148 (MQ=255)
                                                                                                                   aCGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCg                             <  2:2853920/148‑1 (MQ=255)
                                                                                                                     gTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGtt                           <  2:86143/148‑1 (MQ=255)
                                                                                                                      tGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTc                          >  2:2523531/1‑148 (MQ=255)
                                                                                                                      tGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTc                          >  2:352678/1‑148 (MQ=255)
                                                                                                                      tGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTc                          <  2:1369463/148‑1 (MQ=255)
                                                                                                                          aaaaCCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCATTTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCg                      <  1:3538244/148‑1 (MQ=255)
                                                                                                                          aaaaCCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCg                      >  1:2437132/1‑148 (MQ=255)
                                                                                                                           aaaCCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGa                     >  1:2046171/1‑148 (MQ=255)
                                                                                                                           aaaCCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGa                     >  1:2285919/1‑148 (MQ=255)
                                                                                                                            aaCCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGAc                    >  1:1326667/1‑148 (MQ=255)
                                                                                                                               cGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTg                 <  1:3403375/148‑1 (MQ=255)
                                                                                                                               cGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTg                 <  2:1577972/148‑1 (MQ=255)
                                                                                                                                gCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGa                <  1:1980939/148‑1 (MQ=255)
                                                                                                                                gCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGa                <  1:185309/148‑1 (MQ=255)
                                                                                                                                    gtgAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACggg            >  1:2256284/1‑148 (MQ=255)
                                                                                                                                    gtgAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACggg            <  2:628764/148‑1 (MQ=255)
                                                                                                                                     tgAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGc           <  2:1756771/148‑1 (MQ=255)
                                                                                                                                          tGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCAt      >  2:1362325/1‑148 (MQ=255)
                                                                                                                                           ggCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCAtt     >  2:66767/1‑148 (MQ=255)
                                                                                                                                           ggCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCAtt     >  2:1040585/1‑148 (MQ=255)
                                                                                                                                             cTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGc   >  1:2613516/1‑148 (MQ=255)
                                                                                                                                              tGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGca  <  1:1927010/148‑1 (MQ=255)
                                                                                                                                              tGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGca  <  2:51341/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCA  >  CP009273/3479624‑3479913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑