Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,486,738 A→T 41.0% Q271L (CAA→CTA)  tsgA → putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,486,7380AT41.0% 119.5 / 226.8 173Q271L (CAA→CTA) tsgAputative transporter
Reads supporting (aligned to +/- strand):  ref base A (43/59);  new base T (45/26);  total (88/85)
Fisher's exact test for biased strand distribution p-value = 8.42e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 176 total aligned reads displayed.

GTTAGGTTTTATCTCCTGGGTGCCTGAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTGGGTTCAC  >  CP009273/3486592‑3486884
                                                                                                                                                  |                                                                                                                                                  
gTTAGGTTTTATCTCCTGGGTGCCTGAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCaa                                                                                                                                                   >  2:574018/1‑148 (MQ=255)
gTTAGGTTTTATCTCCTGGGTGCCTGAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCaa                                                                                                                                                   >  2:3305564/1‑148 (MQ=255)
gTTAGGTTTTATCTCCTGGGTGCCTGAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCaa                                                                                                                                                   <  2:2560047/148‑1 (MQ=255)
 ttAGGTTTTATCTCCTGGGTGCCTGAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAAc                                                                                                                                                  >  2:494621/1‑148 (MQ=255)
    ggTTTTATCTCCTGGGTGCCTGAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCa                                                                                                                                               >  1:2586068/1‑148 (MQ=255)
    ggTTTTATCTCCTGGGTGCCTGAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCa                                                                                                                                               <  1:138854/148‑1 (MQ=255)
      ttttATCTCCTGGGTGCCTGAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCAtt                                                                                                                                             <  1:3184805/148‑1 (MQ=255)
       tttATCTCCTGGGTGCCTGAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTc                                                                                                                                            <  1:528200/148‑1 (MQ=255)
             tcCTGGGTGCCTGAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCg                                                                                                                                      <  2:1984345/148‑1 (MQ=255)
               cTGGGTGCCTGAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCATCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGTCCGTa                                                                                                                                    <  1:3415876/148‑1 (MQ=255)
               cTGGGTGCCTGAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTa                                                                                                                                    <  1:1817420/148‑1 (MQ=255)
                 gggTGCCTGAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTTCTACGCATTCTGACCGTAct                                                                                                                                  >  1:2719110/1‑148 (MQ=255)
                 gggTGCCTGAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCAATCTGACCGTAct                                                                                                                                  >  1:311287/1‑148 (MQ=255)
                 gggTGCCTGAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCGTCTTTGATTTGCTACGCATTCTGACCGTAct                                                                                                                                  >  1:942026/1‑148 (MQ=255)
                         gAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTc                                                                                                                          <  1:1696650/148‑1 (MQ=255)
                         gAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTc                                                                                                                          >  2:1220886/1‑148 (MQ=255)
                            tATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGCCCGTACTGGCTGGTCTgg                                                                                                                       >  2:1573275/1‑148 (MQ=255)
                                ccAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGc                                                                                                                   >  1:1281602/1‑148 (MQ=255)
                                ccAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGc                                                                                                                   <  1:1246596/148‑1 (MQ=255)
                                  aaaGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGa                                                                                                                 <  2:2925406/148‑1 (MQ=255)
                                    aGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGAtt                                                                                                               >  2:920437/1‑148 (MQ=255)
                                    aGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGAtt                                                                                                               >  1:2425029/1‑148 (MQ=255)
                                     ggCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTc                                                                                                              <  2:2538632/148‑1 (MQ=255)
                                      gCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCt                                                                                                             >  2:1342415/1‑148 (MQ=255)
                                      gCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCt                                                                                                             >  2:1157836/1‑148 (MQ=255)
                                            acATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTa                                                                                                       <  2:3323004/147‑1 (MQ=255)
                                             cATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTAc                                                                                                      >  2:2721052/1‑148 (MQ=255)
                                                gAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTc                                                                                                   >  2:1655431/1‑148 (MQ=255)
                                                 aGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCt                                                                                                  >  2:95905/1‑148 (MQ=255)
                                                     tGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTaa                                                                                              <  2:3457998/148‑1 (MQ=255)
                                                      gAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAac                                                                                             <  1:3109348/148‑1 (MQ=255)
                                                       aaCGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAaca                                                                                            <  1:2666619/148‑1 (MQ=255)
                                                         cgacgCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTAGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACAcc                                                                                          >  1:1051258/1‑148 (MQ=255)
                                                           acgCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCgg                                                                                        >  2:2924406/1‑148 (MQ=255)
                                                            cgcgGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGa                                                                                       <  2:1322456/148‑1 (MQ=255)
                                                                 gCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACAcc                                                                                  >  1:2808891/1‑148 (MQ=255)
                                                                 gCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACAcc                                                                                  <  2:472356/148‑1 (MQ=255)
                                                                  cACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCa                                                                                 >  1:280044/1‑148 (MQ=255)
                                                                   aCGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAg                                                                                <  2:955660/148‑1 (MQ=255)
                                                                   aCGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAg                                                                                >  1:2191016/1‑148 (MQ=255)
                                                                    cGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGc                                                                               >  2:3179254/1‑148 (MQ=255)
                                                                    cGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGc                                                                               <  1:2630723/148‑1 (MQ=255)
                                                                        ggTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACat                                                                           <  2:2810714/148‑1 (MQ=255)
                                                                         gTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACata                                                                          <  1:1800348/148‑1 (MQ=255)
                                                                         gTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACata                                                                          <  1:885635/148‑1 (MQ=255)
                                                                          tGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACatat                                                                         <  2:3153317/148‑1 (MQ=255)
                                                                          tGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACatat                                                                         >  1:2009743/1‑148 (MQ=255)
                                                                            aGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATgg                                                                       <  1:286906/148‑1 (MQ=255)
                                                                            aGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATgg                                                                       >  2:1816995/1‑148 (MQ=255)
                                                                             gTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGc                                                                      <  2:2497917/148‑1 (MQ=255)
                                                                             gTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGc                                                                      <  2:1537371/148‑1 (MQ=255)
                                                                              tAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCg                                                                     >  1:2042832/1‑148 (MQ=255)
                                                                              tAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCg                                                                     >  2:104886/1‑148 (MQ=255)
                                                                               aaCTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGt                                                                    <  2:1278123/148‑1 (MQ=255)
                                                                                aCTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTATGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTg                                                                   >  1:430994/1‑148 (MQ=255)
                                                                                    cTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCa                                                               <  1:188094/148‑1 (MQ=255)
                                                                                      ggATGTCATACATGGTCGGCATGTGGGCGTACAGCTATATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAAt                                                             >  2:876837/1‑148 (MQ=255)
                                                                                       gATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAAtt                                                            <  1:2790524/148‑1 (MQ=255)
                                                                                         tGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATtct                                                          <  1:117638/148‑1 (MQ=255)
                                                                                          gTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCT‑GCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCg                                                        <  1:127953/148‑1 (MQ=255)
                                                                                             aTACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGct                                                      <  2:1178551/148‑1 (MQ=255)
                                                                                             aTACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGct                                                      <  1:3533172/148‑1 (MQ=255)
                                                                                             aTACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGct                                                      <  1:1775865/148‑1 (MQ=255)
                                                                                              tACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGctc                                                     <  2:2586068/148‑1 (MQ=255)
                                                                                              tACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGctc                                                     <  1:339061/148‑1 (MQ=255)
                                                                                               aCATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTTCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATTTACGGCTTTAAAACCCGAACACCAGCACATATGGCGTGGTCAATTATCGctct                                                    >  1:1568846/1‑148 (MQ=255)
                                                                                               aCATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGctct                                                    <  2:3386832/148‑1 (MQ=255)
                                                                                                 aTGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTgg                                                  >  2:3068839/1‑148 (MQ=255)
                                                                                                 aTGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTgg                                                  >  2:2712987/1‑148 (MQ=255)
                                                                                                        gCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGcttctt                                           >  2:238736/1‑148 (MQ=255)
                                                                                                           tgtgGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTTCTACGCATTCTGAACGTACTGGCTGGTATGGCTGCGATTCTGATGTAAGTCTTTAAAACCCGAACACCACCACAAATGGCGTGGTCAATTATCGCTCTGGGCTTCTtctc                                        >  1:1562419/1‑148 (MQ=255)
                                                                                                           tgtgGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTtctc                                        >  2:277981/1‑148 (MQ=255)
                                                                                                           tgtgGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTtctc                                        <  2:3104587/148‑1 (MQ=255)
                                                                                                           tgtgGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTtctc                                        <  2:1910491/148‑1 (MQ=255)
                                                                                                            gtgGGCGTTCAGCTTTATTCGTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTcc                                       <  1:1046192/148‑1 (MQ=255)
                                                                                                             tgGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCa                                      >  1:1915983/1‑148 (MQ=255)
                                                                                                                  gTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGcgcg                                 <  1:1452330/148‑1 (MQ=255)
                                                                                                                   ttCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGa                                >  2:2353627/1‑148 (MQ=255)
                                                                                                                    tCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGAt                               >  1:2905352/1‑148 (MQ=255)
                                                                                                                    tCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGAt                               >  1:1132884/1‑148 (MQ=255)
                                                                                                                     ctggtttATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATc                              <  1:14666/144‑1 (MQ=255)
                                                                                                                     cAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATc                              <  1:470630/148‑1 (MQ=255)
                                                                                                                      aGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCt                             >  1:547514/1‑148 (MQ=255)
                                                                                                                        cTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCtat                           >  2:552841/1‑148 (MQ=255)
                                                                                                                        cTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCtat                           >  2:578583/1‑148 (MQ=255)
                                                                                                                         tttATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCtata                          <  1:2058812/148‑1 (MQ=255)
                                                                                                                         tttATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCtata                          >  1:168056/1‑148 (MQ=255)
                                                                                                                         tttATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCtata                          <  1:574018/148‑1 (MQ=255)
                                                                                                                             ttcttcGCTTCTTTTATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCTATaccac                      >  1:3134717/1‑148 (MQ=255)
                                                                                                                                 tcGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCTATACCACcatc                  <  2:3114787/148‑1 (MQ=255)
                                                                                                                                 tcGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCTATACCACcatc                  >  2:87552/1‑148 (MQ=255)
                                                                                                                                    cttcttTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCTATACCACCatcatc               >  2:3497770/1‑148 (MQ=255)
                                                                                                                                      tcttTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCAc             >  2:3101913/1‑148 (MQ=255)
                                                                                                                                      tcttTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCAc             >  1:783707/1‑148 (MQ=255)
                                                                                                                                        tttGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCAccc           >  2:1332171/1‑148 (MQ=255)
                                                                                                                                        tttGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCAccc           <  2:2985856/148‑1 (MQ=255)
                                                                                                                                             tttGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTGGGt      >  2:704729/1‑148 (MQ=255)
                                                                                                                                             tttGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTGGGt      >  2:1786648/1‑148 (MQ=255)
                                                                                                                                             tttGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTGGGt      >  2:3046256/1‑148 (MQ=255)
                                                                                                                                                gCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTGGGTTca   <  1:657314/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GTTAGGTTTTATCTCCTGGGTGCCTGAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTGGGTTCAC  >  CP009273/3486592‑3486884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑