Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,519,188 C→A 100% L107L (CTG→CTT)  nudE ← adenosine nucleotide hydrolase; substrates include Ap3A, Ap2A, ADP‑ribose, NADH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,519,1880CA95.3% 847.0 / 30.7 298L107L (CTG→CTT) nudEadenosine nucleotide hydrolase; substrates include Ap3A, Ap2A, ADP‑ribose, NADH
Reads supporting (aligned to +/- strand):  ref base C (5/9);  new base A (123/161);  total (128/170)
Fisher's exact test for biased strand distribution p-value = 7.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.54e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 298 total aligned reads displayed.

CATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGATCAGGATCAGG  >  CP009273/3519044‑3519332
                                                                                                                                                |                                                                                                                                                
catATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGa                                                                                                                                               <  2:1700579/148‑1 (MQ=255)
   atGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCg                                                                                                                                            >  1:3311364/1‑148 (MQ=255)
    tGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGt                                                                                                                                           <  2:3233277/148‑1 (MQ=255)
    tGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGt                                                                                                                                           <  1:1439893/148‑1 (MQ=255)
    tGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGt                                                                                                                                           <  2:2572333/148‑1 (MQ=255)
     gcgcCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGtt                                                                                                                                          >  2:2083562/1‑148 (MQ=255)
     gcgcCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGtt                                                                                                                                          <  1:2085860/148‑1 (MQ=255)
      cgcCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTc                                                                                                                                         >  2:2286212/1‑148 (MQ=255)
       gccagcggccagcgCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCg                                                                                                                                        <  2:3153033/148‑1 (MQ=255)
          agcggccagcgCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTc                                                                                                                                     >  2:776225/1‑148 (MQ=255)
          agcggccagcgCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTc                                                                                                                                     >  2:1597905/1‑148 (MQ=255)
          agcggccagcgCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTc                                                                                                                                     <  2:2318662/148‑1 (MQ=255)
          agcggccagcgCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTc                                                                                                                                     <  2:531269/148‑1 (MQ=255)
          agcggccagcgCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTc                                                                                                                                     >  2:494128/1‑148 (MQ=255)
           gcggccagcgCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTcc                                                                                                                                    >  2:2972150/1‑148 (MQ=255)
              gccagcgCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCaaa                                                                                                                                 >  2:3311157/1‑148 (MQ=255)
               ccagcgCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAAt                                                                                                                                >  2:1985981/1‑148 (MQ=255)
                  gcgcACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCa                                                                                                                             >  2:663326/1‑148 (MQ=255)
                   cgcACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCaa                                                                                                                            <  1:1374893/148‑1 (MQ=255)
                       ccTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACctc                                                                                                                        <  1:882536/148‑1 (MQ=255)
                       ccTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACctc                                                                                                                        >  1:675242/1‑148 (MQ=255)
                         tgtgGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCtctt                                                                                                                      <  1:993340/148‑1 (MQ=255)
                         tgtgGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCtctt                                                                                                                      <  1:1707943/148‑1 (MQ=255)
                         tgtgGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCtctt                                                                                                                      <  1:3194080/148‑1 (MQ=255)
                             gTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCttt                                                                                                                  >  2:3355868/1‑148 (MQ=255)
                             gTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCttt                                                                                                                  <  2:1820994/148‑1 (MQ=255)
                               aGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTa                                                                                                                <  2:1871465/148‑1 (MQ=255)
                                      ctGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTcgcg                                                                                                         >  2:1453699/1‑148 (MQ=255)
                                       tGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCGTGCGCTACCACGATAGTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCgg                                                                                                        <  2:2168483/148‑1 (MQ=255)
                                        ggCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGt                                                                                                       <  2:799245/148‑1 (MQ=255)
                                         gCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGtt                                                                                                      >  2:2387690/1‑148 (MQ=255)
                                           tCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAg                                                                                                    <  2:505095/148‑1 (MQ=255)
                                            cATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGc                                                                                                   >  1:2286301/1‑148 (MQ=255)
                                            cATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGc                                                                                                   >  1:496978/1‑148 (MQ=255)
                                                    tCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGt                                                                                           >  1:2005075/1‑148 (MQ=255)
                                                    tCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGt                                                                                           <  2:814869/148‑1 (MQ=255)
                                                      cAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAg                                                                                         <  1:1542771/148‑1 (MQ=255)
                                                       aGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGa                                                                                        <  2:2411542/148‑1 (MQ=255)
                                                         tGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACg                                                                                      <  2:1146507/148‑1 (MQ=255)
                                                         tGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACg                                                                                      >  1:180793/1‑148 (MQ=255)
                                                          gATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGc                                                                                     <  1:1024368/148‑1 (MQ=255)
                                                          gATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGc                                                                                     <  1:2802951/148‑1 (MQ=255)
                                                            ttCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCtt                                                                                   <  1:1476318/148‑1 (MQ=255)
                                                             tCCGGGTAGAGATCTTGCGCTACCGCGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCttt                                                                                  >  1:347235/1‑148 (MQ=255)
                                                             tCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCttt                                                                                  <  1:3311157/148‑1 (MQ=255)
                                                               cGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCa                                                                                <  2:2194459/148‑1 (MQ=255)
                                                                      ataTCTTGGGCTACGACGATATTCATTTTGCTGGAAAAGTAAGTCGGTGCGTTGCTGATCTTCTTCATAAAAGTAAGATCGTTCGCTCCAAATCGATGGTATTCTTTTAGCTCGCGGTTAGCGGGTTCGTAGACGCTTTCACCCGGAt                                                                         <  2:1749973/146‑1 (MQ=255)
                                                                         tCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCaa                                                                      >  1:1954797/1‑148 (MQ=255)
                                                                           ttGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAAtt                                                                    <  1:930223/148‑1 (MQ=255)
                                                                             gcgcTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTaa                                                                  <  1:2512104/148‑1 (MQ=255)
                                                                             gcgcTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTaa                                                                  <  2:36626/148‑1 (MQ=255)
                                                                              cgcTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAAt                                                                 <  2:237990/148‑1 (MQ=255)
                                                                               gcTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATc                                                                <  2:3311364/148‑1 (MQ=255)
                                                                                  aCCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCtt                                                             <  2:3136124/148‑1 (MQ=255)
                                                                                  aCCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCtt                                                             <  1:523727/148‑1 (MQ=255)
                                                                                    cACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTc                                                           >  1:2979760/1‑148 (MQ=255)
                                                                                        atatTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGaaa                                                       <  2:3003363/148‑1 (MQ=255)
                                                                                         tatTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGaaaa                                                      <  1:1000412/148‑1 (MQ=255)
                                                                                         tatTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGaaaa                                                      <  1:494079/148‑1 (MQ=255)
                                                                                          atTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGaaaaa                                                     >  2:3482419/1‑148 (MQ=255)
                                                                                          atTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGaaaaa                                                     >  2:741817/1‑148 (MQ=255)
                                                                                           ttCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAAc                                                    <  2:3363107/148‑1 (MQ=255)
                                                                                           ttCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAAc                                                    <  1:2172101/148‑1 (MQ=255)
                                                                                            tCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAAcc                                                   >  2:1072329/1‑148 (MQ=255)
                                                                                            tCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAAcc                                                   >  2:1639614/1‑148 (MQ=255)
                                                                                               ttttGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTaa                                                <  2:866675/148‑1 (MQ=255)
                                                                                                tttGTTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAAt                                               >  1:2247201/1‑148 (MQ=255)
                                                                                                 ttGCTTGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAATTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAAtt                                              <  2:1869026/148‑1 (MQ=255)
                                                                                                    cTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGt                                           <  1:776225/148‑1 (MQ=255)
                                                                                                     tGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTa                                          <  2:2152945/148‑1 (MQ=255)
                                                                                                     tGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTa                                          >  2:1794427/1‑148 (MQ=255)
                                                                                                     tGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTa                                          >  2:1171795/1‑148 (MQ=255)
                                                                                                      ggAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAg                                         >  1:3172562/1‑148 (MQ=255)
                                                                                                       gAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAgg                                        <  2:3399023/148‑1 (MQ=255)
                                                                                                        aaaaGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGa                                       <  2:2663043/148‑1 (MQ=255)
                                                                                                         aaaGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGAt                                      <  1:2602060/148‑1 (MQ=255)
                                                                                                            gTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCa                                   <  1:2366750/148‑1 (MQ=255)
                                                                                                            gTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCa                                   >  1:2894538/1‑148 (MQ=255)
                                                                                                               aGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGtt                                >  2:452768/1‑148 (MQ=255)
                                                                                                               aGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGtt                                >  1:2954323/1‑148 (MQ=255)
                                                                                                                gACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTc                               >  2:3045167/1‑148 (MQ=255)
                                                                                                                gACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTc                               <  2:1832618/148‑1 (MQ=255)
                                                                                                                gACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTc                               <  2:347235/148‑1 (MQ=255)
                                                                                                                 aCGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTcc                              >  2:713354/1‑148 (MQ=255)
                                                                                                                   ggTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCa                            >  1:1490383/1‑148 (MQ=255)
                                                                                                                   ggTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCa                            <  1:589784/148‑1 (MQ=255)
                                                                                                                    gTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCAc                           <  1:2338392/148‑1 (MQ=255)
                                                                                                                      gCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTg                         <  2:2700763/148‑1 (MQ=255)
                                                                                                                      gCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTg                         >  2:913018/1‑148 (MQ=255)
                                                                                                                           gCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTAt                    <  1:50176/148‑1 (MQ=255)
                                                                                                                           gCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTAt                    <  1:186255/148‑1 (MQ=255)
                                                                                                                               aGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTcgc                <  1:2934703/148‑1 (MQ=255)
                                                                                                                                gCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTcgcg               >  1:428985/1‑148 (MQ=255)
                                                                                                                                 cttcttCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCgg              >  2:2923992/1‑148 (MQ=255)
                                                                                                                                   tcttcAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCggat            <  1:3492647/148‑1 (MQ=255)
                                                                                                                                    cttcAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCggatc           >  1:3437337/1‑148 (MQ=255)
                                                                                                                                      tcAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCggatcag         >  1:2408822/1‑148 (MQ=255)
                                                                                                                                         aaaaaGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGACCAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCggatcaggat      >  2:3511819/1‑148 (MQ=255)
                                                                                                                                           aaaGTAAGATCGTTCGCTCCATATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCggatcaggatca    <  2:1007878/148‑1 (MQ=255)
                                                                                                                                            aaGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGgatcaggatcag   >  1:2810037/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
CATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGATCAGGATCAGG  >  CP009273/3519044‑3519332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑