Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,528,406 G→T 100% I274I (ATC→ATA)  envZ ← sensory histidine kinase in two‑component regulatory system with OmpR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,528,4060GT95.2% 603.6 / 20.5 209I274I (ATC→ATA) envZsensory histidine kinase in two‑component regulatory system with OmpR
Reads supporting (aligned to +/- strand):  ref base G (7/3);  new base T (109/90);  total (116/93)
Fisher's exact test for biased strand distribution p-value = 5.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.56e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 211 total aligned reads displayed.

CGCGGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCGATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACA  >  CP009273/3528262‑3528550
                                                                                                                                                |                                                                                                                                                
 gcgGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTatat                                                                                                                                              <  2:1940453/148‑1 (MQ=255)
     tttCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCttt                                                                                                                                          >  1:1298005/1‑148 (MQ=255)
      ttCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTCGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTa                                                                                                                                         >  2:2854231/1‑148 (MQ=255)
       tCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTAt                                                                                                                                        <  1:1144451/148‑1 (MQ=255)
       tCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTAt                                                                                                                                        <  1:2729908/148‑1 (MQ=255)
        cAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTAtt                                                                                                                                       >  1:1446865/1‑148 (MQ=255)
        cAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTAtt                                                                                                                                       <  2:1118561/148‑1 (MQ=255)
        cAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTAtt                                                                                                                                       >  2:227207/1‑148 (MQ=255)
          aTTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGa                                                                                                                                     >  2:741740/1‑148 (MQ=255)
          aTTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGa                                                                                                                                     <  1:3006621/148‑1 (MQ=255)
           tttCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGAt                                                                                                                                    <  1:257337/148‑1 (MQ=255)
           tttCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGAt                                                                                                                                    >  1:1478046/1‑148 (MQ=255)
           tttCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGAt                                                                                                                                    >  1:1478360/1‑148 (MQ=255)
            ttCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATc                                                                                                                                   >  1:850030/1‑148 (MQ=255)
            ttCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCGTCTATATCTTTATTGATc                                                                                                                                   >  1:2513111/1‑148 (MQ=255)
               ccGCTCATAGCCACTTTCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGAt                                                                                                                                <  1:3328381/148‑1 (MQ=255)
                cGCTCATAGCCACTTTCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGAtt                                                                                                                               <  2:120667/148‑1 (MQ=255)
                cGCTCATAGCCACTTTCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGAtt                                                                                                                               >  2:3135793/1‑148 (MQ=255)
                   tCATAGCCACTTTCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTg                                                                                                                            >  2:2628991/1‑148 (MQ=255)
                    cATAGCCACTTTCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGc                                                                                                                           <  1:440582/148‑1 (MQ=255)
                       aGCCACTTTCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAg                                                                                                                        <  2:1531683/148‑1 (MQ=255)
                       aGCCACTTTCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAg                                                                                                                        <  2:3297835/148‑1 (MQ=255)
                         ccACTTTCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGAt                                                                                                                      <  1:2862869/148‑1 (MQ=255)
                         ccACTTTCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGAt                                                                                                                      <  1:2726213/148‑1 (MQ=255)
                          cACTTTCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATa                                                                                                                     <  2:2803120/148‑1 (MQ=255)
                            cTTTCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGc                                                                                                                   <  1:355751/148‑1 (MQ=255)
                              ttCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCCGATAGCCa                                                                                                                 >  2:2151392/1‑148 (MQ=255)
                              ttCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCa                                                                                                                 >  2:1664976/1‑148 (MQ=255)
                                cGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATc                                                                                                               <  1:3349056/148‑1 (MQ=255)
                                cGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATc                                                                                                               <  1:1587207/148‑1 (MQ=255)
                                cGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCGATATCTTTATTGATCGATTCTGCCAGATAGCCATc                                                                                                               <  1:1844036/148‑1 (MQ=255)
                                     gcaATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGAGAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTgct                                                                                                          >  2:2239612/1‑148 (MQ=255)
                                      caATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTgctc                                                                                                         >  1:3114781/1‑148 (MQ=255)
                                      caATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTgctc                                                                                                         >  2:881030/1‑148 (MQ=255)
                                       aaTCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTgctcg                                                                                                        <  2:1741587/148‑1 (MQ=255)
                                         tcacctcaccGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTgctcgct                                                                                                      >  1:2179967/1‑148 (MQ=255)
                                          cacctccccGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCATGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTgctcgctc                                                                                                     <  2:1380263/148‑1 (MQ=255)
                                            cctcaccGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCtcat                                                                                                   >  2:3073125/1‑148 (MQ=255)
                                             ctcaccGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCtcatc                                                                                                  <  1:2061039/148‑1 (MQ=255)
                                              tcaccGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCtcatca                                                                                                 >  2:2735907/1‑148 (MQ=255)
                                              tcaccGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCtcatca                                                                                                 >  2:3094617/1‑148 (MQ=255)
                                                   gAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCa                                                                                            >  1:2381826/1‑148 (MQ=255)
                                                    aGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAg                                                                                           <  2:2041500/148‑1 (MQ=255)
                                                      tACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTc                                                                                         >  1:900064/1‑148 (MQ=255)
                                                       aCTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCg                                                                                        <  2:1775340/148‑1 (MQ=255)
                                                        cTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGc                                                                                       <  2:2433874/148‑1 (MQ=255)
                                                             ttAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCGATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGc                                                                                  >  1:215466/1‑148 (MQ=255)
                                                              tAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCg                                                                                 >  1:3522899/1‑148 (MQ=255)
                                                              tAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCg                                                                                 >  2:462472/1‑148 (MQ=255)
                                                              tAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCg                                                                                 >  2:462447/1‑148 (MQ=255)
                                                              tAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCg                                                                                 >  1:3237603/1‑148 (MQ=255)
                                                                aGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGaa                                                                               <  1:611305/148‑1 (MQ=255)
                                                                 gATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAAt                                                                              >  1:64227/1‑148 (MQ=255)
                                                                 gATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAAt                                                                              >  1:3267173/1‑148 (MQ=255)
                                                                 gATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAAt                                                                              >  2:3444266/1‑148 (MQ=255)
                                                                 gATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAAt                                                                              >  2:380783/1‑148 (MQ=255)
                                                                 gATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAAt                                                                              >  2:2461383/1‑148 (MQ=255)
                                                                  aTCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATa                                                                             >  1:3190460/1‑148 (MQ=255)
                                                                       ccATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGt                                                                        <  1:912285/148‑1 (MQ=255)
                                                                       ccATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGt                                                                        <  1:2352738/148‑1 (MQ=255)
                                                                         aTTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCa                                                                      >  2:1681256/1‑148 (MQ=255)
                                                                         aTTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCa                                                                      >  2:1833131/1‑148 (MQ=255)
                                                                          tttCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAg                                                                     >  1:1458053/1‑148 (MQ=255)
                                                                           ttCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAgc                                                                    >  2:381718/1‑148 (MQ=255)
                                                                             ccATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAgcgg                                                                  >  2:3107327/1‑148 (MQ=255)
                                                                                tCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGt                                                               >  1:2703592/1‑148 (MQ=255)
                                                                                      tctcCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCaa                                                         >  1:2901859/1‑148 (MQ=255)
                                                                                      tctcCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCaa                                                         >  1:2553773/1‑148 (MQ=255)
                                                                                       ctcCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAg                                                        >  2:3025704/1‑148 (MQ=255)
                                                                                        tcCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGt                                                       <  2:1478155/148‑1 (MQ=255)
                                                                                           tGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGt                                                    >  2:2084459/1‑148 (MQ=255)
                                                                                           tGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGt                                                    >  2:1465456/1‑148 (MQ=255)
                                                                                                           aGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCAt                                    >  2:2121874/1‑148 (MQ=255)
                                                                                                                aTAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATcagca                               <  2:2884234/148‑1 (MQ=255)
                                                                                                                 tAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATcagcag                              >  1:3079792/1‑148 (MQ=255)
                                                                                                                     cTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTg                          <  1:2854231/148‑1 (MQ=255)
                                                                                                                        cTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCgg                       >  2:2105190/1‑148 (MQ=255)
                                                                                                                         tCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGt                      >  2:2575974/1‑148 (MQ=255)
                                                                                                                          cAATGATGGCGTTGCACTCTTCGATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTc                     >  2:2977869/1‑148 (MQ=255)
                                                                                                                            atgatgGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCAt                   >  1:296182/1‑148 (MQ=255)
                                                                                                                            atgatgGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCAt                   >  1:2208207/1‑148 (MQ=255)
                                                                                                                             tgatgGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCATc                  >  1:1450079/1‑148 (MQ=255)
                                                                                                                                 ggCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCATCCGcc              <  1:2965435/148‑1 (MQ=255)
                                                                                                                                 ggCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCATCCGcc              >  2:2747011/1‑148 (MQ=255)
                                                                                                                                   cGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAg            <  2:1152585/148‑1 (MQ=255)
                                                                                                                                     ttGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGtt          >  1:3394794/1‑148 (MQ=255)
                                                                                                                                      tGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTg         >  1:323102/1‑148 (MQ=255)
                                                                                                                                      tGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTg         >  2:1015551/1‑148 (MQ=255)
                                                                                                                                      tGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTg         >  1:1312869/1‑148 (MQ=255)
                                                                                                                                      tGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTg         >  1:1050572/1‑148 (MQ=255)
                                                                                                                                       gCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGc        <  2:2783933/148‑1 (MQ=255)
                                                                                                                                        cACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCt       >  2:2450131/1‑148 (MQ=255)
                                                                                                                                         aCTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCtt      >  2:817673/1‑148 (MQ=255)
                                                                                                                                         aCTCTTCGATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCtt      >  1:79752/1‑148 (MQ=255)
                                                                                                                                          ctctTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTa     <  1:2137345/148‑1 (MQ=255)
                                                                                                                                          ctctTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAAGACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTa     <  1:1014752/148‑1 (MQ=255)
                                                                                                                                           tcttctATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTaa    >  1:1798999/1‑148 (MQ=255)
                                                                                                                                            ctTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAac   >  2:3361497/1‑148 (MQ=255)
                                                                                                                                             ttCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAaca  >  1:1698716/1‑148 (MQ=255)
                                                                                                                                             ttCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAaca  <  1:1649133/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
CGCGGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCGATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACA  >  CP009273/3528262‑3528550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑