Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,545,077 C→A 16.2% L252L (CTG→CTT)  malP ← maltodextrin phosphorylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,545,0770CA16.2% 467.7 / 51.0 160L252L (CTG→CTT) malPmaltodextrin phosphorylase
Reads supporting (aligned to +/- strand):  ref base C (79/55);  new base A (14/12);  total (93/67)
Fisher's exact test for biased strand distribution p-value = 6.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01

Only 100 of 161 total aligned reads displayed.

TAATCCGCCAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACAACGGGGAGATCC  >  CP009273/3544931‑3545224
                                                                                                                                                  |                                                                                                                                                   
tAATCCGCCAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTGTGGATAGAGAACTTTGGTCa                                                                                                                                                    >  1:2234297/1‑148 (MQ=255)
  aTCCGCCAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGt                                                                                                                                                  >  1:2658081/1‑148 (MQ=255)
   tCCGCCAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGtt                                                                                                                                                 >  1:3218424/1‑148 (MQ=255)
   tCCGCCAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGtt                                                                                                                                                 >  1:942559/1‑148 (MQ=255)
       ccAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTc                                                                                                                                             <  1:1868458/148‑1 (MQ=255)
        cAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTcc                                                                                                                                            >  1:602975/1‑148 (MQ=255)
          gTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGc                                                                                                                                          >  1:1845551/1‑148 (MQ=255)
          gTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGc                                                                                                                                          >  1:1845531/1‑148 (MQ=255)
           ttCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCa                                                                                                                                         <  2:624513/148‑1 (MQ=255)
           ttCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTAAGTTTTTCCGCa                                                                                                                                         >  2:1612424/1‑148 (MQ=255)
           ttCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTAAGTTTTTCCGCa                                                                                                                                         >  2:1612431/1‑148 (MQ=255)
                gCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGAt                                                                                                                                    >  1:1025168/1‑148 (MQ=255)
                 cAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATg                                                                                                                                   <  2:2542991/148‑1 (MQ=255)
                      tACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCctg                                                                                                                              >  1:680411/1‑148 (MQ=255)
                      tACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCctg                                                                                                                              >  1:680548/1‑148 (MQ=255)
                         gCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCctgctg                                                                                                                           <  2:1402439/148‑1 (MQ=255)
                         gCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCctgctg                                                                                                                           <  2:9081/148‑1 (MQ=255)
                          ccccGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGt                                                                                                                          <  2:2753847/148‑1 (MQ=255)
                          ccccGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGt                                                                                                                          >  1:3017647/1‑148 (MQ=255)
                             cGCCAGATGATGGCGACGCAAAATATCCGCTGCCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCg                                                                                                                       >  2:3319298/1‑148 (MQ=255)
                              gCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCgg                                                                                                                      >  2:2553437/1‑148 (MQ=255)
                                cAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCa                                                                                                                    <  1:828422/148‑1 (MQ=255)
                                cAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCa                                                                                                                    >  1:154439/1‑148 (MQ=255)
                                 aGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCAc                                                                                                                   >  2:3191704/1‑148 (MQ=255)
                                 aGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCAc                                                                                                                   >  2:831616/1‑148 (MQ=255)
                                   atgatgGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTACGCATTGATGCCCTGCTGTTCGGCACGc                                                                                                                 >  2:1372766/1‑148 (MQ=255)
                                   atgatgGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTAAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGc                                                                                                                 >  2:2385005/1‑148 (MQ=255)
                                   atgatgGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTAAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGc                                                                                                                 >  2:2385002/1‑148 (MQ=255)
                                     gatgGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCaa                                                                                                               >  2:1811168/1‑148 (MQ=255)
                                           gACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATc                                                                                                         <  2:2855047/148‑1 (MQ=255)
                                             cGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTAAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCAc                                                                                                       <  2:3120578/148‑1 (MQ=255)
                                               cAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACcg                                                                                                     <  2:1549822/148‑1 (MQ=255)
                                                aaaaTATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACcgt                                                                                                    <  1:1215760/148‑1 (MQ=255)
                                                  aaTATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACcgtcg                                                                                                  >  1:1016445/1‑148 (MQ=255)
                                                  aaTATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACcgtcg                                                                                                  >  1:1079899/1‑148 (MQ=255)
                                                  aaTATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACcgtcg                                                                                                  >  1:1016544/1‑148 (MQ=255)
                                                      tCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTaa                                                                                              >  2:2942654/1‑148 (MQ=255)
                                                      tCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTAAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTaa                                                                                              >  2:3277140/1‑148 (MQ=255)
                                                        cGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAAt                                                                                            >  2:2351327/1‑148 (MQ=255)
                                                           tACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTAAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTa                                                                                         <  1:2344141/148‑1 (MQ=255)
                                                            aCCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAg                                                                                        >  2:82758/1‑148 (MQ=255)
                                                            aCCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTATTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGATaaaaatag                                                                                        >  2:1300502/1‑143 (MQ=255)
                                                             ccGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGt                                                                                       <  1:574332/148‑1 (MQ=255)
                                                             ccGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTGAAATTTAGt                                                                                       >  1:2868311/1‑148 (MQ=255)
                                                                 aCAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTTGTCAGa                                                                                   >  2:412056/1‑148 (MQ=255)
                                                                  cAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGAt                                                                                  <  2:589918/148‑1 (MQ=255)
                                                                    ggCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCa                                                                                >  2:1417766/1‑148 (MQ=255)
                                                                    ggCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGAATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACAGTCGTTTAAATTAGTCAGATCa                                                                                >  2:2220526/1‑148 (MQ=255)
                                                                     gCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCaa                                                                               >  1:590981/1‑148 (MQ=255)
                                                                     gCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCaa                                                                               >  1:892040/1‑148 (MQ=255)
                                                                     gCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTAAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCaa                                                                               <  2:1895411/148‑1 (MQ=255)
                                                                      cacaCTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCaaa                                                                              <  2:881978/148‑1 (MQ=255)
                                                                        cacTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACg                                                                            >  1:889021/1‑148 (MQ=255)
                                                                         acTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACgg                                                                           <  2:89841/148‑1 (MQ=255)
                                                                          cTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTTAATTTATTGAGATAAAAAGGa                                                                          >  1:96005/1‑148 (MQ=255)
                                                                                gTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGcgcg                                                                    <  2:1285444/148‑1 (MQ=255)
                                                                                        gcATCAGGCGCAGCTTTTTACCGGCCGTATGGTTGTCGTGTGGATAGAGCACTGTGGTAAGTTTTTCCGCTTTGATGCCCGGCGGTTCGGCACGCAAGAAATCCCGGTCGTGAACTTTCGTCAGGTGAAACGGCTGGGGGTGCGTCGc                                                            >  2:2001267/1‑148 (MQ=255)
                                                                                        gcATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGc                                                            <  1:3524992/148‑1 (MQ=255)
                                                                                        gcATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTAAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGc                                                            >  2:2000386/1‑148 (MQ=255)
                                                                                         cATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGcc                                                           >  1:1196616/1‑148 (MQ=255)
                                                                                         cATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAAAGGATGCGCGGGCGTCGcc                                                           >  1:3501135/1‑148 (MQ=255)
                                                                                           tCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGGCGTTAAATTTATTAAGATAAAAAGGATGCGCGTGCGTCGCCTg                                                         >  1:1298103/1‑148 (MQ=255)
                                                                                               gcgcAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCcac                                                     >  2:1091027/1‑148 (MQ=255)
                                                                                                cgcAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTAAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTGAAATTTATTCAGATCAAAAGGATGCGCGTGCGTCGCCTGCccca                                                    >  2:1206168/1‑148 (MQ=255)
                                                                                                 gcAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAg                                                   <  1:2553437/148‑1 (MQ=255)
                                                                                                 gcAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAg                                                   <  2:2930187/148‑1 (MQ=255)
                                                                                                       ttttACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTAAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAg                                             <  2:1946451/148‑1 (MQ=255)
                                                                                                              ggCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCAGTTCGGCACGCAAGAAAACACCGTCGGTAAAAATAGTCCGGTCAAACCGATGCCCCTGCGTGGCCACCCCCAGAcgcagccgcagc                                      >  1:1407417/1‑148 (MQ=255)
                                                                                                               gCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATAAAAAGGATGCGCGTGCGTCGCCTGCCACATACgcagcggcagcg                                     >  1:889836/1‑148 (MQ=255)
                                                                                                                  gTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTATTCAGATCAAAAGGATGCGCGTGCGTCGCCTGCCACACACGCACCCGCTGcgccc                                  >  2:1218629/1‑147 (MQ=255)
                                                                                                                       gTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGcgccacgcca                             <  2:2658081/148‑1 (MQ=255)
                                                                                                                        ttGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATTCGCGTTCGTCGCCTGCCCCAGACGCATCGGCAGCGCCACGCCAt                            >  2:421998/1‑148 (MQ=255)
                                                                                                                         tGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCAtt                           <  1:3026953/148‑1 (MQ=255)
                                                                                                                          gTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTa                          <  1:2672254/148‑1 (MQ=255)
                                                                                                                            cGTTTGGATAGAGAACTTTGGTAAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACg                        <  1:1947629/148‑1 (MQ=255)
                                                                                                                            cGTTTGGATAGAGAACTTTGGTAAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACg                        <  1:1612424/148‑1 (MQ=255)
                                                                                                                            cGTTTGGATAGAGAACTTTGGTAAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCAGCCACAGACGCAGCGGCTGCGCCACGCCATTACg                        >  2:1041777/1‑148 (MQ=255)
                                                                                                                             gTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGa                       <  1:2337361/148‑1 (MQ=255)
                                                                                                                              tttGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTAACATCAAACCGATTCGCGTGCGTCGCCTGCCACAGACACAGCGGCAGCGCCACGCCATTAAGAt                      >  2:105750/1‑148 (MQ=255)
                                                                                                                                 ggATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGc                   <  2:794625/148‑1 (MQ=255)
                                                                                                                                  gATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTATTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGcc                  >  1:3232059/1‑148 (MQ=255)
                                                                                                                                  gATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACCGATGCGCGGGCGTCGCCTGCCACCGACGCACCGGCTGCGCCCCGCCCTTACGATAGcc                  >  1:763382/1‑148 (MQ=255)
                                                                                                                                    tAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGa                <  1:2938781/148‑1 (MQ=255)
                                                                                                                                    tAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGa                <  1:2646930/148‑1 (MQ=255)
                                                                                                                                    tAGAGAACTTTGGTAAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGa                >  2:1179670/1‑148 (MQ=255)
                                                                                                                                     agagAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTAAGATCAAAAGGATGCGCGTGCGTCGCCTGCCCCCGACGCACCGGCTGCGCCACCCCAATAAGATAGCCGAc               >  1:3191622/1‑148 (MQ=255)
                                                                                                                                      gagaACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTTAATTTATTCAGATCAAACGGATGCGCGTGCGTCGCCGGCCACACACCCAGCGGCTGCGCCCCCCCCATAAGATATCCGACa              >  1:3322233/1‑148 (MQ=255)
                                                                                                                                      gagaACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACa              <  1:443985/148‑1 (MQ=255)
                                                                                                                                      gagaACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACa              <  1:444052/148‑1 (MQ=255)
                                                                                                                                      gagaACTTTGGTAAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACa              <  2:3309009/148‑1 (MQ=255)
                                                                                                                                       agaACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATTCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTAAGATAGCCGACaa             >  2:2185947/1‑148 (MQ=255)
                                                                                                                                       agaACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACaa             >  2:2675952/1‑148 (MQ=255)
                                                                                                                                       agaACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACaa             >  2:433156/1‑148 (MQ=255)
                                                                                                                                         aaCTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACAACg           <  1:1125820/148‑1 (MQ=255)
                                                                                                                                         aaCTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACAACg           <  1:1180481/148‑1 (MQ=255)
                                                                                                                                         aaCTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAAATTAGTCAGATCAAAAGGATGCGCGTGCGTCGCCTGCCACAAACGCCGCGGCTGCGCCCCCCCCTTAAGATAGACGACAAcc           >  1:2406598/1‑147 (MQ=255)
                                                                                                                                         aaCTTTGGTAAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACAACg           <  1:2385002/148‑1 (MQ=255)
                                                                                                                                         aaCTTTGGTAAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACAACg           <  1:2384924/148‑1 (MQ=255)
                                                                                                                                            tttGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAAATTAGTCAGATAAAAAGGATGCGCGTGCGTCGCCTGCCACACACCCAGCGGCAGCGCCACCCCAATACGATAGCCGACaaacggg        >  1:2521777/1‑143 (MQ=255)
                                                                                                                                                  cAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATAAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACAACGGGGAGATcc  >  1:2051879/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TAATCCGCCAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACAACGGGGAGATCC  >  CP009273/3544931‑3545224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑