Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,591,957 T→G 100% V354V (GTA→GTC)  livJ ← leucine/isoleucine/valine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,591,9570TG96.8% 740.5 / 3.6 187V354V (GTA→GTC) livJleucine/isoleucine/valine transporter subunit
Reads supporting (aligned to +/- strand):  ref base T (4/2);  new base G (91/90);  total (95/92)
Fisher's exact test for biased strand distribution p-value = 6.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 188 total aligned reads displayed.

TTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCG  >  CP009273/3591813‑3592104
                                                                                                                                                |                                                                                                                                                   
ttGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACg                                                                                                                                                  >  2:1825651/1‑148 (MQ=255)
ttGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACg                                                                                                                                                  >  1:2633061/1‑148 (MQ=255)
ttGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACg                                                                                                                                                  >  1:853480/1‑148 (MQ=255)
    aTCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGa                                                                                                                                              <  1:2788346/148‑1 (MQ=255)
     tCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGaa                                                                                                                                             <  2:2848624/148‑1 (MQ=255)
      cTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAAc                                                                                                                                            >  2:2190397/1‑148 (MQ=255)
        tGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTc                                                                                                                                          >  2:1948511/1‑148 (MQ=255)
        tGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTc                                                                                                                                          >  1:2644823/1‑148 (MQ=255)
           aGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCaaa                                                                                                                                       <  1:401451/148‑1 (MQ=255)
            ggCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAg                                                                                                                                      >  1:1485393/1‑148 (MQ=255)
            ggCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAg                                                                                                                                      >  2:2141823/1‑148 (MQ=255)
             gCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGc                                                                                                                                     >  2:2272559/1‑148 (MQ=255)
             gCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGc                                                                                                                                     >  2:2902398/1‑148 (MQ=255)
               cGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCt                                                                                                                                   <  1:2482006/148‑1 (MQ=255)
               cGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCt                                                                                                                                   <  1:1876174/148‑1 (MQ=255)
               cGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCt                                                                                                                                   <  1:1853780/148‑1 (MQ=255)
                 gATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCttt                                                                                                                                 >  2:1009760/1‑148 (MQ=255)
                 gATAAGGCGTTTACGCCGAATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCAGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCttt                                                                                                                                 <  1:3479819/148‑1 (MQ=255)
                  aTAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTc                                                                                                                                >  1:541365/1‑148 (MQ=255)
                  aTAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTc                                                                                                                                >  2:1662236/1‑148 (MQ=255)
                   tAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCa                                                                                                                               <  1:1443980/148‑1 (MQ=255)
                    aaGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAg                                                                                                                              >  1:3523090/1‑148 (MQ=255)
                    aaGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAg                                                                                                                              <  2:299281/148‑1 (MQ=255)
                    aaGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAg                                                                                                                              <  1:2550382/148‑1 (MQ=255)
                      ggCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGAt                                                                                                                            >  2:2434141/1‑148 (MQ=255)
                               gccgcATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTtct                                                                                                                   <  1:2455699/148‑1 (MQ=255)
                                ccgcATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTtctc                                                                                                                  <  2:3176843/148‑1 (MQ=255)
                                  gcATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCAt                                                                                                                <  1:1478894/148‑1 (MQ=255)
                                      ccGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCa                                                                                                            <  1:3513692/148‑1 (MQ=255)
                                          cATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTc                                                                                                        >  1:1005289/1‑148 (MQ=255)
                                             gAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGc                                                                                                     >  2:1647543/1‑148 (MQ=255)
                                                gCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGt                                                                                                  >  2:2322694/1‑148 (MQ=255)
                                                 cAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTc                                                                                                 <  1:1857423/148‑1 (MQ=255)
                                                   aCGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTccc                                                                                               >  2:1437174/1‑148 (MQ=255)
                                                   aCGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTccc                                                                                               >  1:3172997/1‑148 (MQ=255)
                                                    cGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCa                                                                                              >  1:1884649/1‑148 (MQ=255)
                                                    cGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCa                                                                                              >  1:3010567/1‑148 (MQ=255)
                                                    cGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCa                                                                                              >  1:3113890/1‑148 (MQ=255)
                                                     gTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGNTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCAt                                                                                             <  1:1805226/148‑1 (MQ=255)
                                                       aCTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTa                                                                                           >  2:1981050/1‑148 (MQ=255)
                                                              atTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATc                                                                                    <  1:2404846/148‑1 (MQ=255)
                                                               ttAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATcc                                                                                   >  1:2090538/1‑148 (MQ=255)
                                                                   cAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACgg                                                                               >  2:1041780/1‑148 (MQ=255)
                                                                   cAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACgg                                                                               >  2:344576/1‑148 (MQ=255)
                                                                   cAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACgg                                                                               >  2:1467624/1‑148 (MQ=255)
                                                                    aaTTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGa                                                                              <  1:510776/148‑1 (MQ=255)
                                                                      tttGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGt                                                                            <  2:3392284/148‑1 (MQ=255)
                                                                      tttGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGt                                                                            <  2:853480/148‑1 (MQ=255)
                                                                      tttGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGt                                                                            <  1:1374846/148‑1 (MQ=255)
                                                                      tttGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGt                                                                            <  1:1742275/148‑1 (MQ=255)
                                                                      tttGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGt                                                                            <  2:890154/148‑1 (MQ=255)
                                                                      tttGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGt                                                                            <  2:522638/148‑1 (MQ=255)
                                                                       ttGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGtt                                                                           <  2:2842299/148‑1 (MQ=255)
                                                                         ggcggcAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCg                                                                         <  1:856085/148‑1 (MQ=255)
                                                                         ggcggcAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCg                                                                         <  1:2470092/148‑1 (MQ=255)
                                                                          gcggcAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGc                                                                        <  1:278780/148‑1 (MQ=255)
                                                                            ggcAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCTCtt                                                                      <  1:899991/148‑1 (MQ=255)
                                                                            ggcAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCtt                                                                      <  1:3151420/148‑1 (MQ=255)
                                                                              cAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTc                                                                    >  2:3512345/1‑148 (MQ=255)
                                                                              cAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTc                                                                    >  2:1317089/1‑148 (MQ=255)
                                                                              cAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTc                                                                    <  2:1673318/148‑1 (MQ=255)
                                                                               aaCCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCa                                                                   <  2:1346716/148‑1 (MQ=255)
                                                                                aCCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAg                                                                  >  2:2613498/1‑148 (MQ=255)
                                                                                 cccAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAgg                                                                 <  2:2409727/148‑1 (MQ=255)
                                                                                    aaaGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTAt                                                              <  2:654648/148‑1 (MQ=255)
                                                                                     aaGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTAtt                                                             <  1:2322694/148‑1 (MQ=255)
                                                                                     aaGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTAtt                                                             <  1:2322737/148‑1 (MQ=255)
                                                                                      aGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTAttt                                                            <  2:3402755/148‑1 (MQ=255)
                                                                                      aGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTAttt                                                            <  1:1864573/148‑1 (MQ=255)
                                                                                      aGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTAttt                                                            >  1:1252485/1‑148 (MQ=255)
                                                                                         tGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGc                                                         <  2:263351/148‑1 (MQ=255)
                                                                                         tGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGc                                                         <  2:3073471/148‑1 (MQ=255)
                                                                                           ccGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGa                                                       >  2:335643/1‑148 (MQ=255)
                                                                                           ccGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGa                                                       >  1:2997975/1‑148 (MQ=255)
                                                                                             gATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGAtt                                                     >  1:1298336/1‑148 (MQ=255)
                                                                                              aTTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGAttt                                                    >  1:1983141/1‑148 (MQ=255)
                                                                                                  aTGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGc                                                >  1:747676/1‑148 (MQ=255)
                                                                                                    gATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCg                                              >  1:2949647/1‑148 (MQ=255)
                                                                                                    gATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCg                                              >  1:269379/1‑148 (MQ=255)
                                                                                                      ttACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGa                                            >  2:115293/1‑148 (MQ=255)
                                                                                                      ttACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGa                                            <  1:3104089/148‑1 (MQ=255)
                                                                                                         cTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCg                                         >  2:1519837/1‑148 (MQ=255)
                                                                                                           tCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTc                                       <  2:2173001/148‑1 (MQ=255)
                                                                                                           tCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTc                                       >  1:34315/1‑148 (MQ=255)
                                                                                                                 cGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTg                                 >  2:2491851/1‑148 (MQ=255)
                                                                                                                   gTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGAt                               >  2:1258926/1‑148 (MQ=255)
                                                                                                                    tCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGAtt                              <  2:2829530/148‑1 (MQ=255)
                                                                                                                     cGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTc                             <  1:1157174/148‑1 (MQ=255)
                                                                                                                     cGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTc                             <  1:1157271/148‑1 (MQ=255)
                                                                                                                      gccgtgccgtTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCa                            >  1:1262032/1‑148 (MQ=255)
                                                                                                                        cgtgccgtTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAgg                          >  2:823837/1‑148 (MQ=255)
                                                                                                                             cgtTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGc                     <  2:1805812/148‑1 (MQ=255)
                                                                                                                              gtTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGcc                    <  1:2141823/148‑1 (MQ=255)
                                                                                                                              gtTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGcc                    <  1:1441537/148‑1 (MQ=255)
                                                                                                                                tGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTg                  >  1:1617674/1‑148 (MQ=255)
                                                                                                                                  gCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCa                >  1:513838/1‑148 (MQ=255)
                                                                                                                                      gCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGa            >  2:443194/1‑148 (MQ=255)
                                                                                                                                            cAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAg      <  1:1843812/148‑1 (MQ=255)
                                                                                                                                             aaaGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAgc     <  1:1662236/148‑1 (MQ=255)
                                                                                                                                                tACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGcgcg  >  1:1513411/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
TTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCG  >  CP009273/3591813‑3592104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑