Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,639,239 G→T 100% P165P (CCG→CCT)  rlmJ → 23S rRNA m(6)A2030 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,639,2390GT97.3% 581.5 / 6.2 186P165P (CCG→CCT) rlmJ23S rRNA m(6)A2030 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base G (2/3);  new base T (92/89);  total (94/92)
Fisher's exact test for biased strand distribution p-value = 6.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 189 total aligned reads displayed.

CCGAACTGCACCCGAGCGATTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATCTGGAAGCG  >  CP009273/3639094‑3639383
                                                                                                                                                 |                                                                                                                                                
ccGAACTGCACCCGAGCGATTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACcctcc                                                                                                                                                >  1:701086/1‑148 (MQ=255)
   aaCTGCACCCGAGCGATTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTa                                                                                                                                             >  1:2924264/1‑148 (MQ=255)
      tGCACCCGAGCGATTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTatga                                                                                                                                          >  2:130894/1‑148 (MQ=255)
      tGCACCCGAGCGATTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTatga                                                                                                                                          >  2:2341653/1‑148 (MQ=255)
       gCACCCGAGCGATTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTatgaa                                                                                                                                         <  1:209830/148‑1 (MQ=255)
           ccGAGCGATTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTctgaaatga                                                                                                                                     >  1:3556673/1‑148 (MQ=255)
           ccGAGCGATTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTatgaaatga                                                                                                                                     >  1:2126676/1‑148 (MQ=255)
            cGAGCGATTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTatgaaatgaa                                                                                                                                    >  2:1198884/1‑148 (MQ=255)
              aGCGATTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGaaaa                                                                                                                                  <  1:181275/148‑1 (MQ=255)
                    tACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACt                                                                                                                            >  2:429674/1‑148 (MQ=255)
                    tACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACt                                                                                                                            <  2:1381974/148‑1 (MQ=255)
                    tACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACt                                                                                                                            >  2:1932134/1‑148 (MQ=255)
                    tACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACt                                                                                                                            <  2:646743/148‑1 (MQ=255)
                     aCCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTa                                                                                                                           <  2:1281092/148‑1 (MQ=255)
                     aCCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTa                                                                                                                           <  2:1509325/148‑1 (MQ=255)
                     aCCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTa                                                                                                                           >  1:3147968/1‑148 (MQ=255)
                      cccGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTAt                                                                                                                          <  1:1442794/148‑1 (MQ=255)
                      cccGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTAt                                                                                                                          <  1:1442451/148‑1 (MQ=255)
                       ccGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATc                                                                                                                         <  2:3116363/148‑1 (MQ=255)
                        cGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCa                                                                                                                        <  2:313949/148‑1 (MQ=255)
                          ttgttgCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAg                                                                                                                      <  2:2839186/148‑1 (MQ=255)
                              tgCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCggt                                                                                                                  >  1:2477540/1‑148 (MQ=255)
                              tgCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCggt                                                                                                                  >  1:1510522/1‑148 (MQ=255)
                              tgCGTTCTGAATTTCAGAAAGATAGCAGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAATCTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACACTCCGTAGGAAATGAAAACTGACTATCACGCggt                                                                                                                  >  2:2219768/1‑148 (MQ=255)
                               gCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCggtg                                                                                                                 >  1:2525315/1‑148 (MQ=255)
                                  ttCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTc                                                                                                              >  2:131605/1‑148 (MQ=255)
                                   tCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCa                                                                                                             >  1:2468469/1‑148 (MQ=255)
                                   tCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCa                                                                                                             >  2:1113302/1‑148 (MQ=255)
                                   tCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCa                                                                                                             >  2:2887572/1‑148 (MQ=255)
                                    cTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAg                                                                                                            <  1:1427231/148‑1 (MQ=255)
                                     tGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGc                                                                                                           <  2:3355114/148‑1 (MQ=255)
                                       aaTTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCgg                                                                                                         >  2:3318775/1‑148 (MQ=255)
                                         tttCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGa                                                                                                       <  2:2798909/148‑1 (MQ=255)
                                          ttCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGAt                                                                                                      >  2:559773/1‑148 (MQ=255)
                                             aGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGc                                                                                                   <  2:2016646/148‑1 (MQ=255)
                                                aaGATGGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGa                                                                                                >  1:1959670/1‑148 (MQ=255)
                                                aaGATCGCCGTGCGCGTGTCGAACAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGa                                                                                                <  2:1401917/148‑1 (MQ=255)
                                                  gATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAg                                                                                              >  2:2291055/1‑148 (MQ=255)
                                                  gATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAg                                                                                              <  1:2120004/148‑1 (MQ=255)
                                                   aTAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAgg                                                                                             <  2:3239498/148‑1 (MQ=255)
                                                    tAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGt                                                                                            <  1:2669631/148‑1 (MQ=255)
                                                    tAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGt                                                                                            <  1:1750677/148‑1 (MQ=255)
                                                        cGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACa                                                                                        >  1:3195948/1‑148 (MQ=255)
                                                        cGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACa                                                                                        >  1:749398/1‑148 (MQ=255)
                                                         gTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACaa                                                                                       <  1:1085209/148‑1 (MQ=255)
                                                         gTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACaa                                                                                       >  1:293753/1‑148 (MQ=255)
                                                         gTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACaa                                                                                       <  1:1971413/148‑1 (MQ=255)
                                                          tGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACaaa                                                                                      <  2:2953072/148‑1 (MQ=255)
                                                            cgcgTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACg                                                                                    >  1:1619971/1‑148 (MQ=255)
                                                              cgTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGtt                                                                                  >  1:2615087/1‑148 (MQ=255)
                                                                tgtCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTc                                                                                >  1:2222759/1‑148 (MQ=255)
                                                                 gtCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCg                                                                               <  1:3506429/148‑1 (MQ=255)
                                                                 gtCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCg                                                                               <  1:1749008/148‑1 (MQ=255)
                                                                 gtCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCg                                                                               <  2:673569/148‑1 (MQ=255)
                                                                    gAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCa                                                                            <  2:2503581/148‑1 (MQ=255)
                                                                      aaaaGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACt                                                                          >  1:3120613/1‑148 (MQ=255)
                                                                         aGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGt                                                                       >  1:1364741/1‑148 (MQ=255)
                                                                          gCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTa                                                                      <  1:64624/148‑1 (MQ=255)
                                                                           ccGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTAt                                                                     <  1:2983089/148‑1 (MQ=255)
                                                                             gACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTAttt                                                                   >  1:3454691/1‑148 (MQ=255)
                                                                               cGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTAc                                                                 >  1:942443/1‑148 (MQ=255)
                                                                                ggTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACg                                                                <  1:1932134/148‑1 (MQ=255)
                                                                                  tttCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCa                                                              <  2:2191651/148‑1 (MQ=255)
                                                                                     ccAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACtg                                                           <  1:477123/148‑1 (MQ=255)
                                                                                     ccAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACtg                                                           >  1:2647200/1‑148 (MQ=255)
                                                                                         cagcTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGt                                                       <  1:2291055/148‑1 (MQ=255)
                                                                                           gcTGAAGGCCAAACTGCCGCCGGTGTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTAt                                                     <  2:2172140/148‑1 (MQ=255)
                                                                                                aGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCggt                                                <  1:3430027/148‑1 (MQ=255)
                                                                                                 ggCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCggtg                                               >  1:2036448/1‑148 (MQ=255)
                                                                                                     aaaCTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGtgc                                           <  2:3014433/148‑1 (MQ=255)
                                                                                                     aaaCTGCCGCCGGTTTCCCGCCGTGGTTTAATACTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTTCGCACTGTGGTATCCGGTGGtgc                                           <  1:2626917/148‑1 (MQ=255)
                                                                                                        cTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGtgctgc                                        >  2:2924884/1‑148 (MQ=255)
                                                                                                          gccgccGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGt                                      <  2:1587841/148‑1 (MQ=255)
                                                                                                          gccgccGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGt                                      <  1:2423032/148‑1 (MQ=255)
                                                                                                          gccgccGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGt                                      <  2:2373956/148‑1 (MQ=255)
                                                                                                          gccgccGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGt                                      <  1:635522/148‑1 (MQ=255)
                                                                                                           ccgccgGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTc                                     >  1:3092979/1‑148 (MQ=255)
                                                                                                           ccgccgGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTc                                     >  2:3025761/1‑148 (MQ=255)
                                                                                                                  tttCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAAt                              <  1:2902423/148‑1 (MQ=255)
                                                                                                                   ttCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAAtt                             >  1:526634/1‑148 (MQ=255)
                                                                                                                   ttCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAAtt                             >  1:82429/1‑148 (MQ=255)
                                                                                                                        gccgTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAgcg                        <  2:378646/148‑1 (MQ=255)
                                                                                                                         ccgTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAgcgc                       <  2:899306/148‑1 (MQ=255)
                                                                                                                           gTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCAt                     >  1:706738/1‑148 (MQ=255)
                                                                                                                            tGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATg                    >  2:3261680/1‑148 (MQ=255)
                                                                                                                            tGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATGTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATg                    <  1:2715667/148‑1 (MQ=255)
                                                                                                                              gTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGAt                  <  1:1198884/148‑1 (MQ=255)
                                                                                                                               tttAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATc                 <  2:3425316/148‑1 (MQ=255)
                                                                                                                                ttAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATcc                >  1:41524/1‑148 (MQ=255)
                                                                                                                                   aTCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACg             >  2:1611898/1‑148 (MQ=255)
                                                                                                                                    tCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGa            <  2:701098/148‑1 (MQ=255)
                                                                                                                                       ttATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATCt         <  1:130894/148‑1 (MQ=255)
                                                                                                                                        tATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATCTg        <  2:543670/148‑1 (MQ=255)
                                                                                                                                         aTCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATCTgg       <  2:1112668/148‑1 (MQ=255)
                                                                                                                                         aTCGACCCGCCGTATGATATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATCTgg       <  2:965368/148‑1 (MQ=255)
                                                                                                                                          tCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATCTGGa      >  2:912443/1‑148 (MQ=255)
                                                                                                                                             aCCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATCTGGAAGc   >  1:52533/1‑148 (MQ=255)
                                                                                                                                             aCCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATCTGGAAGc   >  1:3395051/1‑148 (MQ=255)
                                                                                                                                              cccTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATCTGGAAGCg  >  2:2567140/1‑148 (MQ=255)
                                                                                                                                              cccTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATCTGGAAGCg  <  2:2115560/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CCGAACTGCACCCGAGCGATTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATCTGGAAGCG  >  CP009273/3639094‑3639383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑