Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 300,770 C→A 100% V49V (GTG→GTT)  ecpE ← ECP production pilus chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273300,7700CA95.1% 607.5 / 21.0 204V49V (GTG→GTT) ecpEECP production pilus chaperone
Reads supporting (aligned to +/- strand):  ref base C (6/4);  new base A (116/78);  total (122/82)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 206 total aligned reads displayed.

ACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACA  >  CP009273/300625‑300915
                                                                                                                                                 |                                                                                                                                                 
aCTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAAc                                                                                                                                                 >  1:2828025/1‑148 (MQ=255)
aCTCGCTCCCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAAc                                                                                                                                                 >  1:2669408/1‑148 (MQ=255)
 cTCGCTCTCCCCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACt                                                                                                                                                <  1:2287794/148‑1 (MQ=255)
 cTCGCTCTCACCAGCCTGCATCGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCAGCGGTCGGGTGCGCAATTCACTGCTGCCCTGGCTATCAATAGCACTGATGGCAATACTGTATATCCGTGCGCTTTTGTTGTTATTAACt                                                                                                                                                <  1:2236178/148‑1 (MQ=255)
 cTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACt                                                                                                                                                <  1:2883999/148‑1 (MQ=255)
 cTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACt                                                                                                                                                >  1:3491947/1‑148 (MQ=255)
   cGCTCTCCCCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCGATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTac                                                                                                                                              <  1:1842261/148‑1 (MQ=255)
    gCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTaca                                                                                                                                             <  1:2050003/148‑1 (MQ=255)
    gCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTaca                                                                                                                                             >  1:1475346/1‑148 (MQ=255)
    gCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTaca                                                                                                                                             <  2:2201112/148‑1 (MQ=255)
       ctcACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGt                                                                                                                                          >  1:1712375/1‑148 (MQ=255)
       ctcACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGt                                                                                                                                          >  1:2170695/1‑148 (MQ=255)
           ccAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGc                                                                                                                                      >  1:1452664/1‑148 (MQ=255)
           ccAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGc                                                                                                                                      >  1:3219108/1‑148 (MQ=255)
             aGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTg                                                                                                                                    >  1:528895/1‑148 (MQ=255)
             aGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCAACCACCCGTCGGGTGCGCAATTCACTGCTCCCCGGGCTATCAATAGCACTGAAGGCAATAAGCTATATCCGTGCGCTTTTGTTGATATTCACTACACGTTAGCTg                                                                                                                                    >  1:528965/1‑148 (MQ=255)
              gCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGa                                                                                                                                   >  2:2635324/1‑148 (MQ=255)
              gCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGa                                                                                                                                   <  1:850138/148‑1 (MQ=255)
              gCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGa                                                                                                                                   <  1:850177/148‑1 (MQ=255)
                    aaCGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAagt                                                                                                                             <  2:2557725/148‑1 (MQ=255)
                      cGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAagtca                                                                                                                           <  1:2716706/148‑1 (MQ=255)
                          aGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGtct                                                                                                                       <  1:553353/148‑1 (MQ=255)
                          aGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGtct                                                                                                                       <  2:2138937/148‑1 (MQ=255)
                            cTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCg                                                                                                                     >  1:2336287/1‑148 (MQ=255)
                                 ggggggCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCACTAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACgg                                                                                                                <  2:1052548/148‑1 (MQ=255)
                                  gggggCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGc                                                                                                               <  1:1972501/148‑1 (MQ=255)
                                  gggggCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGc                                                                                                               <  1:175477/148‑1 (MQ=255)
                                  gggggCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGc                                                                                                               >  2:1922064/1‑148 (MQ=255)
                                           aaGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAAt                                                                                                      >  1:1191690/1‑148 (MQ=255)
                                            aGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATg                                                                                                     >  1:692942/1‑148 (MQ=255)
                                             gCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGt                                                                                                    >  1:2667808/1‑148 (MQ=255)
                                             gCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGt                                                                                                    >  1:934429/1‑148 (MQ=255)
                                                 ttCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGa                                                                                                <  1:3201431/148‑1 (MQ=255)
                                                 ttCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGa                                                                                                >  1:1372853/1‑148 (MQ=255)
                                                  tCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGAt                                                                                               <  1:1227431/148‑1 (MQ=255)
                                                    aCCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTg                                                                                             <  1:244503/148‑1 (MQ=255)
                                                    aCCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTg                                                                                             >  1:916497/1‑148 (MQ=255)
                                                       aTCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGccg                                                                                          >  1:420728/1‑148 (MQ=255)
                                                            ccGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGccgaccga                                                                                     >  1:1111652/1‑148 (MQ=255)
                                                             cGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGaa                                                                                    <  2:240309/148‑1 (MQ=255)
                                                             cGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGAGGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGaa                                                                                    <  2:3322964/148‑1 (MQ=255)
                                                                ccGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATc                                                                                 >  2:2986807/2‑148 (MQ=255)
                                                                   ggTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGcc                                                                              >  2:2331810/1‑148 (MQ=255)
                                                                     tGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCtg                                                                            >  1:1285361/1‑148 (MQ=255)
                                                                           aTTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCaa                                                                      >  2:866275/1‑148 (MQ=255)
                                                                           aTTCACTGCTGCCCGGGCTAGCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCaa                                                                      <  2:2926497/148‑1 (MQ=255)
                                                                            ttCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCaaa                                                                     <  2:1621683/148‑1 (MQ=255)
                                                                             tCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAg                                                                    >  2:739138/1‑148 (MQ=255)
                                                                                  gctgcCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTgcc                                                               >  2:1237173/1‑148 (MQ=255)
                                                                                  gctgcCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTGTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTgcc                                                               >  2:2606382/1‑148 (MQ=255)
                                                                                     gcCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTgccgcc                                                            >  1:316474/1‑148 (MQ=255)
                                                                                      cccGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCa                                                           >  2:2899312/1‑148 (MQ=255)
                                                                                      cccGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCa                                                           >  1:2837884/1‑148 (MQ=255)
                                                                                       ccGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAg                                                          <  1:2624144/148‑1 (MQ=255)
                                                                                        cGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGc                                                         >  1:562573/1‑148 (MQ=255)
                                                                                          ggCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCAt                                                       >  1:2153823/1‑148 (MQ=255)
                                                                                           gCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATa                                                      <  2:3029969/148‑1 (MQ=255)
                                                                                             tATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACa                                                    >  2:2848446/1‑148 (MQ=255)
                                                                                             tATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACa                                                    <  1:1200155/148‑1 (MQ=255)
                                                                                              aTCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAg                                                   >  1:3521416/1‑148 (MQ=255)
                                                                                              aTCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTATGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGACGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAg                                                   >  1:2705356/1‑148 (MQ=255)
                                                                                               tCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGa                                                  <  2:575352/148‑1 (MQ=255)
                                                                                                   tAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGc                                              >  2:1285050/1‑148 (MQ=255)
                                                                                                   tAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGc                                              <  2:1444043/148‑1 (MQ=255)
                                                                                                    aGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCt                                             >  2:1714259/1‑148 (MQ=255)
                                                                                                      cACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGt                                           >  1:904827/1‑148 (MQ=255)
                                                                                                       aCTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTc                                          <  2:1285943/148‑1 (MQ=255)
                                                                                                       aCTGATGGCAATACGGGATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTc                                          <  2:1285361/148‑1 (MQ=255)
                                                                                                         tGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTcag                                        <  1:1362603/148‑1 (MQ=255)
                                                                                                         tGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTcag                                        <  1:2139839/148‑1 (MQ=255)
                                                                                                         tGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTcag                                        >  2:2375746/1‑148 (MQ=255)
                                                                                                          gATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTcagc                                       >  1:1256703/1‑148 (MQ=255)
                                                                                                          gATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTcagc                                       >  2:2200078/1‑148 (MQ=255)
                                                                                                             ggCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAgg                                    <  2:118641/148‑1 (MQ=255)
                                                                                                               cAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGc                                  <  2:2190890/148‑1 (MQ=255)
                                                                                                                   aCGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCGGTCAGCAGGGCCTTa                              >  2:1833505/1‑148 (MQ=255)
                                                                                                                     ggTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGt                            <  2:3219108/148‑1 (MQ=255)
                                                                                                                      gTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGtt                           >  1:3440662/1‑148 (MQ=255)
                                                                                                                      gTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGtt                           >  1:2260281/1‑148 (MQ=255)
                                                                                                                        atatCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTaa                         <  1:891001/148‑1 (MQ=255)
                                                                                                                         tatCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAAt                        <  1:2986807/148‑1 (MQ=255)
                                                                                                                         tatCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAAt                        <  1:2611856/148‑1 (MQ=255)
                                                                                                                         tatCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAAt                        >  1:2552471/1‑148 (MQ=255)
                                                                                                                          atCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATg                       >  2:2756926/1‑148 (MQ=255)
                                                                                                                            ccGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGtt                     <  2:1475346/148‑1 (MQ=255)
                                                                                                                            ccGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGtt                     >  1:3501600/1‑148 (MQ=255)
                                                                                                                               tGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACg                  >  2:22815/1‑148 (MQ=255)
                                                                                                                                gcgcTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGc                 >  1:2826108/1‑148 (MQ=255)
                                                                                                                                gcgcTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGc                 >  2:2341882/1‑148 (MQ=255)
                                                                                                                                gcgcTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGc                 >  2:3467569/1‑148 (MQ=255)
                                                                                                                                    ttttGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACg             >  2:523799/1‑148 (MQ=255)
                                                                                                                                        gttgttATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGc         <  2:3379640/148‑1 (MQ=255)
                                                                                                                                         ttgttATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGcc        >  1:199292/1‑148 (MQ=255)
                                                                                                                                         ttgttATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGcc        >  2:2951787/1‑148 (MQ=255)
                                                                                                                                          tgttATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCt       >  1:1633110/1‑148 (MQ=255)
                                                                                                                                          tgttATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCt       <  1:2392651/148‑1 (MQ=255)
                                                                                                                                            ttattaACTACACGTTTGCTGACAAAGTCAGTCTAGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGa     >  2:2337132/1‑148 (MQ=255)
                                                                                                                                             tATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGaa    >  1:600724/1‑148 (MQ=255)
                                                                                                                                             tATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGaa    >  1:2869004/1‑148 (MQ=255)
                                                                                                                                              aTTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAAc   >  1:2175399/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
ACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACA  >  CP009273/300625‑300915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑