Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,679,324 C→A 100% M1070I (ATG→ATT)  bcsC ← cellulose synthase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,679,3240CA99.4% 555.5 / ‑4.2 168M1070I (ATG→ATT) bcsCcellulose synthase subunit
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (111/56);  total (111/57)
Fisher's exact test for biased strand distribution p-value = 3.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 170 total aligned reads displayed.

GGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCTGCCAGTCGGTCGGGATCAGATTCATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAACCG  >  CP009273/3679179‑3679468
                                                                                                                                                 |                                                                                                                                                
ggATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATtaat                                                                                                                                                <  1:2806228/148‑1 (MQ=255)
   tatCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAg                                                                                                                                             <  1:838383/148‑1 (MQ=255)
   tatCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAg                                                                                                                                             <  2:3208315/148‑1 (MQ=255)
    atCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGc                                                                                                                                            >  1:1314143/1‑148 (MQ=255)
     tCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCTGCCAGTCGGTCGGGATCAGATTCATCAGCg                                                                                                                                           <  1:2953245/148‑1 (MQ=255)
     tCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCg                                                                                                                                           <  2:2565577/148‑1 (MQ=255)
       aaTTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTGAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGa                                                                                                                                         <  2:1844332/148‑1 (MQ=255)
        aTTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGAt                                                                                                                                        <  2:2029563/148‑1 (MQ=255)
         ttGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATa                                                                                                                                       >  1:2582395/1‑148 (MQ=255)
         ttGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATa                                                                                                                                       <  2:755146/148‑1 (MQ=255)
          tGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATaa                                                                                                                                      >  1:239636/1‑148 (MQ=255)
               tGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGTTTAATCAGCGGATAACGCgg                                                                                                                                 <  1:2361180/148‑1 (MQ=255)
                gCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGc                                                                                                                                >  1:2604846/1‑148 (MQ=255)
                 ccAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCa                                                                                                                               >  2:477780/1‑148 (MQ=255)
                  caacaaACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCAt                                                                                                                              >  1:3182180/1‑148 (MQ=255)
                   aacaaACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATg                                                                                                                             >  1:2657820/1‑148 (MQ=255)
                   aacaaACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATg                                                                                                                             >  2:2840197/1‑148 (MQ=255)
                    acaaACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATgg                                                                                                                            >  2:778795/1‑148 (MQ=255)
                     caaACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGt                                                                                                                           >  2:260666/1‑148 (MQ=255)
                     caaACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGt                                                                                                                           >  1:2710352/1‑148 (MQ=255)
                       aaCCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGttt                                                                                                                         >  2:743354/1‑148 (MQ=255)
                       aaCCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGttt                                                                                                                         >  2:2528453/1‑148 (MQ=255)
                        aCCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGtttt                                                                                                                        <  2:1437418/148‑1 (MQ=255)
                        aCCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGtttt                                                                                                                        >  1:1084373/1‑148 (MQ=255)
                          cAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTgg                                                                                                                      >  1:347808/1‑148 (MQ=255)
                               ggAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGt                                                                                                                 >  1:745207/1‑148 (MQ=255)
                                gAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTg                                                                                                                >  2:1973559/1‑148 (MQ=255)
                                  aGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGaa                                                                                                              >  1:3404623/1‑148 (MQ=255)
                                  aGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGaa                                                                                                              <  1:1790413/148‑1 (MQ=255)
                                   gTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAAt                                                                                                             <  2:321983/148‑1 (MQ=255)
                                    tAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATg                                                                                                            <  1:1399596/148‑1 (MQ=255)
                                    tAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATg                                                                                                            >  1:3120802/1‑148 (MQ=255)
                                      acacGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCg                                                                                                          <  2:2464026/148‑1 (MQ=255)
                                      acacGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCg                                                                                                          <  2:1593358/148‑1 (MQ=255)
                                      acacGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCg                                                                                                          >  1:2018162/1‑148 (MQ=255)
                                      acacGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCg                                                                                                          <  2:2464264/148‑1 (MQ=255)
                                             gtTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGa                                                                                                   <  1:3304227/148‑1 (MQ=255)
                                             gtTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGa                                                                                                   >  1:3383566/1‑148 (MQ=255)
                                               tCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGc                                                                                                 <  2:538777/148‑1 (MQ=255)
                                               tCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGc                                                                                                 <  1:2757423/148‑1 (MQ=255)
                                                 aaGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCa                                                                                               >  1:1007863/1‑148 (MQ=255)
                                                   gTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGa                                                                                             >  1:2779643/1‑148 (MQ=255)
                                                    tAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGAc                                                                                            >  1:1156561/1‑148 (MQ=255)
                                                      aTGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGc                                                                                          >  2:2355099/1‑148 (MQ=255)
                                                       tGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCa                                                                                         >  2:821826/1‑148 (MQ=255)
                                                       tGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCa                                                                                         >  1:2465466/1‑148 (MQ=255)
                                                        gCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCAc                                                                                        >  1:2666559/1‑148 (MQ=255)
                                                        gCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCAc                                                                                        >  1:2666572/1‑148 (MQ=255)
                                                        gCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCAc                                                                                        >  1:2666581/1‑148 (MQ=255)
                                                             cgcCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGc                                                                                   >  2:2895286/1‑148 (MQ=255)
                                                               ccGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCAGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTc                                                                                 >  2:2838867/1‑148 (MQ=255)
                                                                   gtAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCAc                                                                             >  2:2237744/1‑148 (MQ=255)
                                                                       ccGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGAcc                                                                         >  2:435526/1‑148 (MQ=255)
                                                                       ccGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGAcc                                                                         >  1:2443370/1‑148 (MQ=255)
                                                                           aGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGtt                                                                     >  1:1307453/1‑148 (MQ=255)
                                                                           aGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGtt                                                                     >  2:503564/1‑148 (MQ=255)
                                                                           aGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGtt                                                                     >  2:2260692/1‑148 (MQ=255)
                                                                            gCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGttt                                                                    >  2:1615399/1‑148 (MQ=255)
                                                                            gCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGttt                                                                    >  2:3366491/1‑148 (MQ=255)
                                                                             cccTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGtttt                                                                   <  1:246864/148‑1 (MQ=255)
                                                                              ccTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTc                                                                  >  2:2622625/1‑148 (MQ=255)
                                                                              ccTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTc                                                                  <  2:1373722/148‑1 (MQ=255)
                                                                               cTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTcc                                                                 <  1:216747/148‑1 (MQ=255)
                                                                                 gACTGCTGCCGCCCTCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGt                                                               >  1:1587364/1‑148 (MQ=255)
                                                                                 gACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGt                                                               >  1:2424623/1‑148 (MQ=255)
                                                                                 gACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGt                                                               >  1:778919/1‑148 (MQ=255)
                                                                                 gACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGt                                                               >  1:2560533/1‑148 (MQ=255)
                                                                                    tgctgcCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTgcg                                                            >  1:3018756/1‑148 (MQ=255)
                                                                                    tgctgcCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTGTTGGTGCGTGAATGCGACCACGAGCCAGACGCTCCCAGCTCCCACGACCAGTTTTCCGTgcg                                                            >  2:2803234/1‑148 (MQ=255)
                                                                                      ctgcCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCt                                                          >  2:1730428/1‑148 (MQ=255)
                                                                                        gccgccATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTcc                                                        <  2:3276138/148‑1 (MQ=255)
                                                                                        gccgccATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTcc                                                        <  2:3016105/148‑1 (MQ=255)
                                                                                        gccgccATCGTTGGATTGCCGCGCAGCGTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGTTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTcc                                                        <  2:3217136/148‑1 (MQ=255)
                                                                                           gccATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGc                                                     <  2:1624552/148‑1 (MQ=255)
                                                                                            ccATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGcc                                                    >  1:420518/1‑148 (MQ=255)
                                                                                               tCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCcaca                                                 >  2:1663934/1‑148 (MQ=255)
                                                                                                  ttGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCa                                              >  1:1472064/1‑148 (MQ=255)
                                                                                                   tGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCAc                                             <  1:2355099/148‑1 (MQ=255)
                                                                                                      aTTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCgg                                          >  2:163977/1‑148 (MQ=255)
                                                                                                       ttGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGt                                         <  1:708558/148‑1 (MQ=255)
                                                                                                          ccGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATg                                      >  1:1434994/1‑148 (MQ=255)
                                                                                                          ccGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATg                                      <  2:182296/148‑1 (MQ=255)
                                                                                                          ccGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATg                                      >  2:715118/1‑148 (MQ=255)
                                                                                                          ccGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATg                                      >  1:3203897/1‑148 (MQ=255)
                                                                                                            gcgcAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGc                                    <  2:3238301/148‑1 (MQ=255)
                                                                                                             cgcAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCa                                   >  2:1830733/1‑148 (MQ=255)
                                                                                                             cgcAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCa                                   >  2:1013049/1‑148 (MQ=255)
                                                                                                              gcAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCaa                                  <  2:1798887/148‑1 (MQ=255)
                                                                                                               cAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCaaa                                 <  2:1294000/148‑1 (MQ=255)
                                                                                                                 gCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACg                               >  1:188390/1‑148 (MQ=255)
                                                                                                                        ccggCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTa                        >  2:3103512/4‑148 (MQ=255)
                                                                                                                                       ggATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTgta         >  2:3483576/1‑148 (MQ=255)
                                                                                                                                       ggATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTgta         >  2:2108094/1‑148 (MQ=255)
                                                                                                                                       ggATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTgta         >  2:299528/1‑148 (MQ=255)
                                                                                                                                         aTCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTgtagt       >  1:2050489/1‑148 (MQ=255)
                                                                                                                                          tCATATTAATCTGCGGATACCGCGGCTTGTTTTGGGTGCGTTACGGCGACCACGAGCCAGACCCACCCAGCTCCCACGACCAGTTTTCGGGGCGCCCCCCCCACATCACCGGTAGGGCACACGACAGGTATTCCTGCGGACTgtagta      <  1:401302/148‑1 (MQ=255)
                                                                                                                                           cAGATTAATCCGCGGATACCGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTaa     <  2:565997/148‑1 (MQ=255)
                                                                                                                                             gATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAAcc   >  2:357508/1‑148 (MQ=255)
                                                                                                                                             gATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAAcc   >  1:3273851/1‑148 (MQ=255)
                                                                                                                                              aTTAATCAGCGTATACCGCGGAATGGTTTTGGTGCGTGAAGGCGACCACGACCCAGACGCACCCAGCTCCCACCACCTTTTTTCCGGGCGCACCCGCCACATCACGGTTATGGAAAACGACAGGTATTCCTGCGGACTGTAGTAACCg  <  1:2222501/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCTGCCAGTCGGTCGGGATCAGATTCATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAACCG  >  CP009273/3679179‑3679468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑