Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,686,345 C→A 100% R751L (CGC→CTC)  bcsA ← cellulose synthase, catalytic subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,686,3450CA98.9% 574.7 / ‑1.8 176R751L (CGC→CTC) bcsAcellulose synthase, catalytic subunit
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (76/98);  total (77/99)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 176 total aligned reads displayed.

CCAGAGCGCCCATGTATCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGCGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCTTCGCGGGCAATTGCCGCGGGCATCGTC  >  CP009273/3686200‑3686488
                                                                                                                                                 |                                                                                                                                               
ccAGAGCGCCCATGTATCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGt                                                                                                                                               <  1:533718/148‑1 (MQ=255)
   gagCGCCCATGTATCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTa                                                                                                                                            >  1:255581/1‑148 (MQ=255)
    agCGCCCATGTATCCGCACGGGCGAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTATTTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTaa                                                                                                                                           <  2:898396/148‑1 (MQ=255)
    agCGCCCATGTATCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTaa                                                                                                                                           <  2:361916/148‑1 (MQ=255)
     gcgcCCATGTATCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAg                                                                                                                                          <  2:3368639/148‑1 (MQ=255)
          cATGTATCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACa                                                                                                                                     <  1:2281796/148‑1 (MQ=255)
           aTGTATCCGCACGGGCAAACGTACACTGCACAAAATCGATAGGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAg                                                                                                                                    <  1:2503762/148‑1 (MQ=255)
            tGTATCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGa                                                                                                                                   <  1:794238/148‑1 (MQ=255)
            tGTATCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGa                                                                                                                                   <  2:2163935/148‑1 (MQ=255)
            tGTATCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGa                                                                                                                                   <  1:794293/148‑1 (MQ=255)
            tGTATCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGa                                                                                                                                   <  1:3223936/148‑1 (MQ=255)
            tGTATCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGa                                                                                                                                   >  1:1934812/1‑148 (MQ=255)
             gTATCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGAt                                                                                                                                  >  2:3380642/1‑148 (MQ=255)
              tATCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGAtt                                                                                                                                 >  1:2058685/1‑148 (MQ=255)
              tATCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGAtt                                                                                                                                 >  1:2384673/1‑148 (MQ=255)
              tATCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGAtt                                                                                                                                 <  1:1930062/148‑1 (MQ=255)
               aTCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTc                                                                                                                                <  1:1825654/148‑1 (MQ=255)
               aTCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTc                                                                                                                                <  1:397675/148‑1 (MQ=255)
                 ccGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCAc                                                                                                                              <  1:2668805/148‑1 (MQ=255)
                    cACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACttt                                                                                                                           >  1:2919727/1‑148 (MQ=255)
                    cACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACttt                                                                                                                           <  2:2992996/148‑1 (MQ=255)
                    cACGGGCAAACGTACACTGCACAAAATCGAGATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACttt                                                                                                                           <  1:2430817/148‑1 (MQ=255)
                      cGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGACGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCt                                                                                                                         >  1:820473/1‑148 (MQ=255)
                      cGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCt                                                                                                                         >  1:2601224/1‑148 (MQ=255)
                           aaaCGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCt                                                                                                                    <  1:643789/148‑1 (MQ=255)
                             aCGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTc                                                                                                                  <  1:501597/148‑1 (MQ=255)
                             aCGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAAGTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTc                                                                                                                  <  1:2137061/148‑1 (MQ=255)
                                tACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAg                                                                                                               <  1:3408642/148‑1 (MQ=255)
                                tACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAg                                                                                                               <  1:939782/148‑1 (MQ=255)
                                 acacTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGa                                                                                                              <  2:163268/148‑1 (MQ=255)
                                 acacTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGa                                                                                                              >  2:2596000/1‑148 (MQ=255)
                                   acTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAAt                                                                                                            >  2:1313060/1‑148 (MQ=255)
                                     tGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCt                                                                                                          <  1:3303925/148‑1 (MQ=255)
                                     tGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCt                                                                                                          >  2:3373279/1‑148 (MQ=255)
                                      gCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTg                                                                                                         <  2:2969364/148‑1 (MQ=255)
                                      gCACAAAATCGATATGGTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTg                                                                                                         <  2:2969395/148‑1 (MQ=255)
                                         caAAATCGAGATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTgcgc                                                                                                      <  2:3050738/148‑1 (MQ=255)
                                          aaaaTCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGCGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGcc                                                                                                     >  2:1800155/1‑148 (MQ=255)
                                            aaTCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTg                                                                                                   >  1:744378/1‑148 (MQ=255)
                                                gATAGGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCg                                                                                               <  2:1838453/148‑1 (MQ=255)
                                                    tGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGttga                                                                                           >  1:1125503/1‑148 (MQ=255)
                                                        gCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGttgatctt                                                                                       >  2:2115274/1‑148 (MQ=255)
                                                        gCTGGGTGGTGAGCGACATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGttgatctt                                                                                       >  1:280232/1‑148 (MQ=255)
                                                            ggtggtGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGttgatcttgatc                                                                                   >  2:2779880/1‑148 (MQ=255)
                                                               ggtgAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATcccc                                                                                <  2:3109784/148‑1 (MQ=255)
                                                               ggtgAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATcccc                                                                                <  2:3111397/148‑1 (MQ=255)
                                                               ggtgAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATcccc                                                                                <  2:3440317/148‑1 (MQ=255)
                                                                     cGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAacca                                                                          >  1:658271/1‑148 (MQ=255)
                                                                       gCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAaccacc                                                                        <  1:2021842/148‑1 (MQ=255)
                                                                         aTTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGt                                                                      >  2:1128136/1‑148 (MQ=255)
                                                                          ttAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTc                                                                     >  2:2711055/1‑148 (MQ=255)
                                                                          ttAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTc                                                                     >  2:875714/1‑148 (MQ=255)
                                                                          ttAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTc                                                                     <  1:2966966/148‑1 (MQ=255)
                                                                           tAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCg                                                                    >  2:3452430/1‑148 (MQ=255)
                                                                           tAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCg                                                                    >  2:3546250/1‑148 (MQ=255)
                                                                             aTTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTCGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCATAAACTGCGCCTGACCGTTGATCATGATCACCAAACCACCGTCGga                                                                  >  1:464855/1‑148 (MQ=255)
                                                                                 cAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGaaa                                                              <  2:2526785/148‑1 (MQ=255)
                                                                                   gCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATc                                                            >  2:2056600/1‑148 (MQ=255)
                                                                                    cccAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATcc                                                           >  1:3015453/1‑148 (MQ=255)
                                                                                    cccAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATcc                                                           <  2:127654/148‑1 (MQ=255)
                                                                                    cccAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATcc                                                           <  2:127694/148‑1 (MQ=255)
                                                                                     ccAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCt                                                          <  1:2193452/148‑1 (MQ=255)
                                                                                     ccAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCt                                                          <  1:321116/148‑1 (MQ=255)
                                                                                          ttCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACg                                                     >  1:2150584/1‑148 (MQ=255)
                                                                                           tCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACgg                                                    <  1:1534926/148‑1 (MQ=255)
                                                                                               tACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTaca                                                >  1:2881572/1‑148 (MQ=255)
                                                                                                 cccATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACg                                              >  2:693331/1‑148 (MQ=255)
                                                                                                  ccATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACga                                             >  2:343075/1‑148 (MQ=255)
                                                                                                   cATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACgag                                            <  2:3289690/148‑1 (MQ=255)
                                                                                                    aTCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACgaga                                           <  1:65628/148‑1 (MQ=255)
                                                                                                    aTCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACgaga                                           >  2:3439100/1‑148 (MQ=255)
                                                                                                     tCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGagaa                                          <  1:86753/148‑1 (MQ=255)
                                                                                                      cACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGagaag                                         <  2:1537303/148‑1 (MQ=255)
                                                                                                      cACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGagaag                                         <  2:1536975/148‑1 (MQ=255)
                                                                                                        cgcgcgCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAagag                                       >  1:3475681/1‑148 (MQ=255)
                                                                                                        cgcgcgCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAagag                                       >  1:263247/1‑148 (MQ=255)
                                                                                                          cgcgcCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGCGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGt                                     <  1:1725717/148‑1 (MQ=255)
                                                                                                             gcCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGAc                                  <  2:2319800/148‑1 (MQ=255)
                                                                                                                aCCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCAt                               <  1:3030940/148‑1 (MQ=255)
                                                                                                                aCCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCACCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCAt                               <  2:1729366/148‑1 (MQ=255)
                                                                                                                 ccTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATc                              <  2:1935361/148‑1 (MQ=255)
                                                                                                                 ccTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATc                              >  2:1192791/1‑148 (MQ=255)
                                                                                                                  cTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCt                             <  2:1367255/148‑1 (MQ=255)
                                                                                                                       tCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCCAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCTTcgcg                        >  2:2417826/1‑148 (MQ=255)
                                                                                                                       tCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCTTcgcg                        <  1:921694/148‑1 (MQ=255)
                                                                                                                       tCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCTTcgcg                        <  1:1595364/148‑1 (MQ=255)
                                                                                                                           gAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCTTCGCGGGCa                    >  1:1596840/1‑148 (MQ=255)
                                                                                                                           gAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCTTCGCGGGCa                    >  2:3123982/1‑148 (MQ=255)
                                                                                                                           gAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCTTCGCGGGCa                    >  1:3357296/1‑148 (MQ=255)
                                                                                                                            aaGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCTTCGCGGGCaa                   <  1:3380642/148‑1 (MQ=255)
                                                                                                                            aaGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCTTCGCGGGCaa                   >  2:2131865/1‑148 (MQ=255)
                                                                                                                                   aTTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCTTCGCGGGCAATTGCcgc            >  1:2559423/1‑148 (MQ=255)
                                                                                                                                     tCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCTTCGCGGGCAATTGCCGCgg          <  1:557005/148‑1 (MQ=255)
                                                                                                                                     tCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCTTCGCGGGCAATTGCCGCgg          <  1:2529238/148‑1 (MQ=255)
                                                                                                                                     tCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCTTCGCGGGCAATTGCCGCgg          >  1:3486596/1‑148 (MQ=255)
                                                                                                                                        tgctgACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCTTCGCGGGCAATTGCCGCGGGCa       <  2:495686/148‑1 (MQ=255)
                                                                                                                                         gctgACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCTTCGCGGGCAATTGCCGCGGGCAt      >  1:1646980/1‑148 (MQ=255)
                                                                                                                                             aCCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCTTCGCGGGCAATTGCCGCGGGCATCGTc  <  1:3282264/148‑1 (MQ=255)
                                                                                                                                             aCCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCTTCGCGGGCAATTGCCGCGGGCATCGTc  >  1:2815622/1‑148 (MQ=255)
                                                                                                                                             aCCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCTTCGCGGGCAATTGCCGCGGGCATCGTc  >  1:1284337/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CCAGAGCGCCCATGTATCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGCGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCTTCGCGGGCAATTGCCGCGGGCATCGTC  >  CP009273/3686200‑3686488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑