Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,696,327 A→T 44.2% T294T (ACT→ACA)  dppD ← dipeptide/heme transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,696,3270AT44.2% 77.9 / 274.0 190T294T (ACT→ACA) dppDdipeptide/heme transporter
Reads supporting (aligned to +/- strand):  ref base A (55/51);  new base T (53/31);  total (108/82)
Fisher's exact test for biased strand distribution p-value = 1.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

Only 100 of 196 total aligned reads displayed.

ATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAACGACGCCAGACGTTCTTTGTCCTGAGCAAATTCTGGCAGCGCACGCAGCAATGCCTGAGTATACGGGTGACG  >  CP009273/3696182‑3696473
                                                                                                                                                 |                                                                                                                                                  
aTAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATctgtctgt                                                                                                                                                  <  2:1554533/148‑1 (MQ=255)
 tAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCtgtg                                                                                                                                                 >  1:1057243/1‑148 (MQ=255)
 tAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCtgtg                                                                                                                                                 >  1:2446081/1‑148 (MQ=255)
 tAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTg                                                                                                                                                 >  1:2733844/1‑148 (MQ=255)
   gCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGc                                                                                                                                               >  2:2507362/1‑148 (MQ=255)
       gCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCTGTGGCATAg                                                                                                                                           <  2:1814244/148‑1 (MQ=255)
          aCAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCTGTGGCATAgggg                                                                                                                                        >  1:3216480/1‑148 (MQ=255)
          aCAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCTGTGGCATAgggg                                                                                                                                        >  1:3107787/1‑148 (MQ=255)
          aCAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTAAGCGCCGGTTCTTCAGCGCGACATCTGTCTGTGGCATAgggg                                                                                                                                        >  1:1448757/1‑148 (MQ=255)
                gttgttGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCTGTGGCATAGGGGCAgcgc                                                                                                                                  >  2:3274951/1‑148 (MQ=255)
                  tgttgCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCATAGGGGCAGCGCgg                                                                                                                                <  1:2482628/148‑1 (MQ=255)
                  tgttgCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCATAGGGGCAGCGCgg                                                                                                                                <  1:3059191/148‑1 (MQ=255)
                   gttgCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCATAGGGGCAGCGCggg                                                                                                                               <  1:3331725/148‑1 (MQ=255)
                       cAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCTGTGGCATAGGGGCAGCGCGGGTTaa                                                                                                                           >  2:2909559/1‑148 (MQ=255)
                         gggTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCTGTGGCATAGGGGCAGCGCGGGTTAAgc                                                                                                                         <  2:1612753/148‑1 (MQ=255)
                         gggTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCTGTGGCATAGGGGCAGCGCGGGTTAAgc                                                                                                                         <  2:3375669/148‑1 (MQ=255)
                          ggTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCATTGGCATAGGGGCAGCGCGGGTTAAgca                                                                                                                        >  2:2812050/1‑148 (MQ=255)
                          ggTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCATAGGGGCAGCGCGGGTTAAgca                                                                                                                        >  1:3062673/1‑148 (MQ=255)
                             ggCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCTGTGGCATAGGGGCAGCGCGGGTTAAgcaggc                                                                                                                     <  1:3441195/148‑1 (MQ=255)
                              gCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCTGTGGCATAGGGGCAGCGCGGGTTAAgcaggca                                                                                                                    >  2:3356283/1‑148 (MQ=255)
                                 tctTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCATCAGCGCGACATCTGTCAGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGcc                                                                                                                 >  1:3206046/1‑148 (MQ=255)
                                    tGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGtt                                                                                                              <  2:360696/148‑1 (MQ=255)
                                         aCTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCATAGGGGCCGCGCGGGTTAAGCAGGCAGCCGTTCGGGc                                                                                                         >  1:421464/1‑148 (MQ=255)
                                           tCATAGTGTCGGCCTCCCGGCAACATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCgg                                                                                                       <  1:688497/148‑1 (MQ=255)
                                            cATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGt                                                                                                      <  1:1127313/148‑1 (MQ=255)
                                              tAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCTGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCg                                                                                                    <  1:2285929/148‑1 (MQ=255)
                                               aGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGt                                                                                                   >  2:1482112/1‑148 (MQ=255)
                                                gtgtCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTa                                                                                                  <  2:2520355/148‑1 (MQ=255)
                                                   tCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCTGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACtt                                                                                               >  1:634594/1‑148 (MQ=255)
                                                      gCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCTGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGcc                                                                                            >  2:1300206/1‑148 (MQ=255)
                                                         tCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCTGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCggg                                                                                         >  1:118779/1‑148 (MQ=255)
                                                         tCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCggg                                                                                         <  2:2804240/148‑1 (MQ=255)
                                                          cccGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCTGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGa                                                                                        <  1:118751/148‑1 (MQ=255)
                                                             ggCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCTGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACg                                                                                     >  1:3340340/1‑148 (MQ=255)
                                                              gCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGa                                                                                    <  2:346565/148‑1 (MQ=255)
                                                              gCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGa                                                                                    <  2:346516/148‑1 (MQ=255)
                                                                 tcatcaAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCATAGGGGCCGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGacac                                                                                 >  1:1418607/1‑148 (MQ=255)
                                                                 tcatcaAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGacac                                                                                 >  2:1703748/1‑148 (MQ=255)
                                                                  catcaAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCTGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACAcc                                                                                >  2:1063250/1‑148 (MQ=255)
                                                                  catcaAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCTGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACAcc                                                                                >  1:903393/1‑148 (MQ=255)
                                                                  catcaAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCTGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACAcc                                                                                >  1:3274076/1‑148 (MQ=255)
                                                                   atcaAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCTTTCGGGCGGTCGTACTTGCCGGGAACGACACCt                                                                               <  1:921415/148‑1 (MQ=255)
                                                                       aGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCa                                                                           <  2:2435659/148‑1 (MQ=255)
                                                                       aGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCa                                                                           >  2:2602522/1‑148 (MQ=255)
                                                                         tGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCTGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAac                                                                         >  1:932159/1‑148 (MQ=255)
                                                                         tGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAac                                                                         >  1:744461/1‑148 (MQ=255)
                                                                           ggTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATCTTAAGCGCCGGTTCTTCAGCGCGACATCTGTCTGTGGCATAGGGGCAGCGCGGGTTATGCTGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAacga                                                                       <  2:1523263/148‑1 (MQ=255)
                                                                            gTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAacgac                                                                      >  2:1843977/1‑148 (MQ=255)
                                                                               aTGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAACGACGcc                                                                   >  2:3326210/1‑148 (MQ=255)
                                                                                    aTTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCTGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAACGACGCCAGACg                                                              <  1:2602629/148‑1 (MQ=255)
                                                                                     tttGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAACGACGCCAGACGt                                                             <  2:2982326/148‑1 (MQ=255)
                                                                                     tttGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAACGACGCCAGACGt                                                             <  2:1208476/148‑1 (MQ=255)
                                                                                      ttGGACTGACGCCCGTCAGCGAGCATATTCCGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTAGTTGCCGGGAACGACACCTGGCAACGACGCCAGACGtt                                                            <  1:623378/148‑1 (MQ=255)
                                                                                      ttGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAACGACGCCTGACGtt                                                            <  1:3231428/148‑1 (MQ=255)
                                                                                      ttGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAACGACGCCAGACGtt                                                            <  2:2306441/148‑1 (MQ=255)
                                                                                      ttGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAACGACGCCAGACGtt                                                            >  2:1288330/1‑148 (MQ=255)
                                                                                        ggACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCTGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAACGACGCCAGACGTTCt                                                          <  2:2404420/148‑1 (MQ=255)
                                                                                           cTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAACGACGCCAGACGTTCTTTg                                                       <  1:533170/148‑1 (MQ=255)
                                                                                            tGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAACGACGCCAGACGTTCTTTGt                                                      <  2:3060860/148‑1 (MQ=255)
                                                                                             gACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAACGACGCCAGACGTTCTTTGTc                                                     <  1:1940674/148‑1 (MQ=255)
                                                                                             gACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCATAAGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAACGACGCCAGACGTTCTTTGTc                                                     <  2:3382093/148‑1 (MQ=255)
                                                                                              aCGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCTGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAACGACGCCAGACGTTCTTTGTcc                                                    >  2:623813/1‑148 (MQ=255)
                                                                                                gCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAACGACGCCAGACGTTCTTTGTCCTg                                                  >  2:22423/1‑148 (MQ=255)
                                                                                                 cccGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCTGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAACGACGCCAGACGTTCTTTGTCCTGa                                                 <  1:1539332/148‑1 (MQ=255)
                                                                                                      cAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCTGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAACGACGCCAGACGTTCTTTGTCCTGAGCaaa                                            <  2:2170468/148‑1 (MQ=255)
                                                                                                      cAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAACGACGCCAGACGTTCTTTGTCCTGAGCaaa                                            <  2:2660519/148‑1 (MQ=255)
                                                                                                       aGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCTGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAACGACGCCAGACGTTCTTTGTCCTGAGCAAAt                                           >  2:2576153/1‑148 (MQ=255)
                                                                                                       aGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAACGACGCCAGACGTTCTTTGTCCTGAGCAAAt                                           <  2:82728/148‑1 (MQ=255)
                                                                                                        gCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAACGACGCCAGACGTTCTTTGTCCTGAGCAAAtt                                          <  1:3378390/148‑1 (MQ=255)
                                                                                                          gAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCTGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAACGACGCCAGACGTTCTTTGTCCTGAGCAAATTCt                                        <  1:1866557/148‑1 (MQ=255)
                                                                                                           aGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCTGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAACGACGCCAGACGTTCTTTGTCCTGAGCAAATTCTg                                       >  2:1376104/1‑148 (MQ=255)
                                                                                                            gCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAACGACGCCAGACGTTCTTTGTCCTGAGCAAATTCTgg                                      >  2:69705/1‑148 (MQ=255)
                                                                                                            gCATATTCAGCGCCGGTTCTAAAGCGCGACATATGTCATTGGCATATTGGCAGCGCGGGTTAAGCAGGAAGACGTTCGGGCGTTCGTAATTGCCGGGAACGACACATGGCAACGACGCCAGACGTTATATGTCATGAGCAAATTCTgt                                      >  1:2707250/1‑147 (MQ=255)
                                                                                                             cATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCTGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAACGACGCCAGACGTTCTTTGTCCTGAGCAAATTCTGGc                                     >  1:1894622/1‑148 (MQ=255)
                                                                                                               tatTCAGCGCCGGTTCTTCAGCGCGACATCTGTCTGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAACGACGCCAGACGTTCTTTGTCCTGAGCAAATTCTGGCAg                                   >  1:2778151/1‑148 (MQ=255)
                                                                                                               tatTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAACGACGCCAGACGTTCTTTGTCCTGAGCAAATTCTGGCAg                                   >  1:2899620/1‑148 (MQ=255)
                                                                                                                 ttCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAACGACGCCAGACGTTCTTTGTCCTGAGCAAATTCTGGCAgcg                                 >  1:227373/1‑148 (MQ=255)
                                                                                                                 ttCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAACGACGCCAGACGTTCTTTGTCCTGAGCAAATTCTGGCAgag                                 >  1:2025495/1‑146 (MQ=255)
                                                                                                                  tCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCATAGGGGCAGCGCGGGTTAAGCCGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAACGACGCCAGACGTTCTTTGTCCTGAGCAAATTCTGGCAgcgc                                >  2:2029245/1‑148 (MQ=255)
                                                                                                                     gcgcCGGTTCTTCAGCGCGACATCTGTCTGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAACGACGCCAGACGTTCTTTGTCCTGAGCAAATTCTGGCAGcgcacg                             >  2:3411331/1‑148 (MQ=255)
                                                                                                                      cgcCGGTTCTTCAGCGCGACATCTGTCAGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAACGACGCCAGACGTTCTTTGTCCTGAGCAAATTCTGGCAGcgcacgc                            >  1:1429871/1‑148 (MQ=255)
                                                                                                                        ccGGTTCTTCAGCGCGACATCTGTCTGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAACGACGCCAGACGTTCTTTGTCCTGAGCAAATTCTGGCAGCGCACgcag                          <  2:634594/148‑1 (MQ=255)
                                                                                                                         cGGTTCTTCAGCGCGACATCTGTCTGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAACGACGCCAGACGTTCTTTGTCCTGAGCAAATTCTGGCAGCGCACgcagc                         >  1:1243733/1‑148 (MQ=255)
                                                                                                                           gTTCTTCAGCGCGACATCTGTCTGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAACGACGCCAGACGTTCTTTGTCCTGAGCAAATTCTGGCAGCGCACGCAGCaa                       <  1:2353032/148‑1 (MQ=255)
                                                                                                                           gTTCTTCAGCGCGACATCTGTCTGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAACGACGCCAGACGTTCTTTGTCCTGAGCAAATTCTGGCAGCGCACGCAGCaa                       >  2:3298708/1‑148 (MQ=255)
                                                                                                                           gTTCTTCAGCGCGACATCTGTCTGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAACGACGCCAGACGTTCTTTGTCCTGAGCAAATTCTGGCAGCGCACGCAGCaa                       >  1:1337499/1‑148 (MQ=255)
                                                                                                                            ttcttcAGCGCGACATCTGTCAGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGACCGACACCTGGCAACGACGCCAGACGTTCTTTGTCCTGAGCAAATTCTGGCAGCGCACGCAGCAAt                      >  2:469098/1‑148 (MQ=255)
                                                                                                                             tcttcAGCGCGACATCTGTCAGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAACGACGCCAGACGTTCTTTGTCCTGAGCAAATTCTGGCAGCGCACGCAGCAATg                     >  1:2266280/1‑148 (MQ=255)
                                                                                                                             tcttcAGCGCGACATCTGTCAGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAACGACGCCAGACGTTCTTTGTCCTGAGCAAATTCTGGCAGCGCACGCAGCAATg                     >  1:2762763/1‑148 (MQ=255)
                                                                                                                              cttcAGCGCGACATCTGTCTGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAACGACGCCAGACGTTCTTTGTCCTGAGCAAATTCTGGCAGCGCACGCAGCAATGc                    >  1:2408427/1‑148 (MQ=255)
                                                                                                                              cttcAGCGCGACATCTGTCAGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAACGACGCCAGACGTTCTTTGTCCTGAGCAAATTCTGGCAGCGCACGCAGCAATGc                    <  2:2733657/148‑1 (MQ=255)
                                                                                                                               ttcAGCGCGACCTCTGTCAGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAACGACGCCAGACGTTCTTTGTCCTGAGCAAATTCTGGCAGCGCACGCAGCAATGcc                   <  2:704948/148‑1 (MQ=255)
                                                                                                                                tcAGCGCGACATCTGTCTGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAACGACGCCAGACGTTCTTTGTCCTGAGCAAATTCTGGCAGCGCACGCAGCAATGCCt                  >  1:2630076/1‑148 (MQ=255)
                                                                                                                                tcAGCGCGACATCTGTCAGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAACGACGCCAGACGTTCTTTGTCCTGAGCAAATTCTGGCAGCGCACGCAGCAATGCCt                  >  1:1018556/1‑148 (MQ=255)
                                                                                                                                 cAGCGCGACATCTGTCAGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAACGACGCCAGACGTTCTTTGTCCTGAGCAAATTCTGGCAGCGCACGCAGCAATGCCTg                 >  1:1041464/1‑148 (MQ=255)
                                                                                                                                   gcgcGACATCTGTCTGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAACGACGCCAGACGTTCTTTGTCCTGAGCAAATTCTGGCAGCGCACGCAGCAATGCCTGAg               >  1:2655232/1‑148 (MQ=255)
                                                                                                                                     gcgACATCTGTCAGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAACGACGCCAGACGTTCTTTGTCCTGAGCAAATTCTGGCAGCGCACGCAGCAATGCCTGAGta             <  1:1505496/148‑1 (MQ=255)
                                                                                                                                      cgACATCTGTCAGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAACGACGCCAGACGTTCTTTGTCCTGAGCAAATTCTGGCAGCGCACGCAGCAATGCCTGAGtat            >  2:3038866/1‑148 (MQ=255)
                                                                                                                                         cATCTGTCAGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAACGACGCCAGACGTTCTTTGTCCTGAGCAAATTCTGGCAGCGCACGCAGCAATGCCTGAGTATACg         <  1:158712/148‑1 (MQ=255)
                                                                                                                                                cAGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAACGACGCCAGACGTTCTTTGTCCTGAGCAAATTCTGGCAGCGCACGCAGCAATGCCTGAGTATACGGGTGAcg  <  2:1992269/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
ATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCATAGGGGCAGCGCGGGTTAAGCAGGCAGCCGTTCGGGCGGTCGTACTTGCCGGGAACGACACCTGGCAACGACGCCAGACGTTCTTTGTCCTGAGCAAATTCTGGCAGCGCACGCAGCAATGCCTGAGTATACGGGTGACG  >  CP009273/3696182‑3696473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑