Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 309,680 C→T 10.9% intergenic (‑164/‑388) ykgP ← / → eaeH pseudogene, oxidoreductase family/pseudogene, attaching and effacing protein homology;factor; Not classified

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273309,6800CT10.9% 739.4 / 56.5 230intergenic (‑164/‑388)ykgP/eaeHpseudogene, oxidoreductase family/pseudogene, attaching and effacing protein homology;factor; Not classified
Reads supporting (aligned to +/- strand):  ref base C (97/108);  new base T (14/11);  total (111/119)
Fisher's exact test for biased strand distribution p-value = 5.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 236 total aligned reads displayed.

AAGCATAACGTAAAGGGAAAAGCTCGATTAGACGGCAGAATTTGTCAGGGGTTATGAACGAAATTCATAAATCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTAT  >  CP009273/309533‑309827
                                                                                                                                                   |                                                                                                                                                   
 aGCATAACGTAAAGGGAAAAGCTCGATTAGACGGCAGAATTTGTCAGGGGTTATGAACGAAATTCATAAATCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATcc                                                                                                                                                    <  1:1881653/148‑1 (MQ=255)
 aGCATAACGTAAAGGGAAAAGCTCGATTAGACGGCAGAATTTGTCAGGGGTTATGAACGAAATTCATAAATCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATcc                                                                                                                                                    <  1:1287621/148‑1 (MQ=255)
  gCATAACGTAAAGGGAAAAGCTCGATTAGACGGCAGAATTTGTCAGGGGTTATGAACGAAATTCATAAATCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATTCa                                                                                                                                                   >  2:1750860/1‑148 (MQ=255)
  gCATAACGTAAAGGGAAAAGCTCGATTAGACGGCAGAATTTGTCAGGGGTTATGAACGAAATTCATAAATCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCa                                                                                                                                                   >  2:1375681/1‑148 (MQ=255)
  gCATAACGTAAAGGGAAAAGCTCGATTAGACGGCAGAATTTGTCAGGGGTTATGAACGAAATTCATAAATCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCa                                                                                                                                                   >  2:1375713/1‑148 (MQ=255)
   cATAACGTAAAGGGAAAAGCTCGATTAGACGGCAGAATTTGTCAGGGGTTATGAACGAAATTCATAAATCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCAt                                                                                                                                                  <  1:398323/148‑1 (MQ=255)
     tAACGTAAAGGGAAAAGCTCGATTAGACGGCAGAATTTGTCAGGGGTTATGAACGAAATTCATAAATCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATTCATaa                                                                                                                                                >  1:2576983/1‑148 (MQ=255)
     tAACGTAAAGGGAAAAGCTCGATTAGACGGCAGAATTTGTCAGGGGTTATGAACGAAATTCATAAATCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATaa                                                                                                                                                <  1:1703006/148‑1 (MQ=255)
        cGTAAAGGGAAAAGCTCGATTAGACGGCAGAATTTGTCAGGGGTTATGAACGAAATTCATAAATCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATaaaaa                                                                                                                                             >  2:770614/1‑148 (MQ=255)
          tAAAGGGAAAAGCTCGATTAGACGGCAGAATTTGTCAGGGGTTATGAACGAAATTCATAAATCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAat                                                                                                                                           <  2:2600940/148‑1 (MQ=255)
            aaGGGAAAAGCTCGATTAGACGGCAGAATTTGTCAGGGGTTATGAACGAAATTCATAAATCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAatat                                                                                                                                         <  1:1589195/148‑1 (MQ=255)
            aaGGGAAAAGCTCGATTAGACGGCAGAATTTGTCAGGGGTTATGAACGAAATTCATAAATCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAatat                                                                                                                                         >  1:3230855/1‑148 (MQ=255)
            aaGGGAAAAGCTCGATTAGACGGCAGAATTTGTCAGGGGTTATGAACGAAATTCATAAATCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAatat                                                                                                                                         <  2:881299/148‑1 (MQ=255)
            aaGGGAAAAGCTCGATTAGACGGCAGAATTTGTCAGGGGTTATGAACGAAATTCATAAATCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAatat                                                                                                                                         >  1:493181/1‑148 (MQ=255)
              gggAAAAGCTCGATTAGACGGCAGAATTTGTCAGGGGTTATGAACGAAATTCATAAATCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAatatat                                                                                                                                       >  1:2751380/1‑148 (MQ=255)
                 aaaaGCTCGATTAGACGGCAGAATTTGTCAGGGGTTATGAACGAAATTCATAAATCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATTCATAAAAAATATATTgg                                                                                                                                    <  2:1237178/148‑1 (MQ=255)
                  aaaGCTCGATTAGACGGCAGAATTTGTCAGGGGTTATGAACGAAATTCATAAATCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGt                                                                                                                                   <  1:1811390/148‑1 (MQ=255)
                  aaaGCTCGATTAGACGGCAGAATTTGTCAGGGGTTATGAACGAAATTCATAAATCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGt                                                                                                                                   >  2:786056/1‑148 (MQ=255)
                   aaGCTCGATTAGACGGCAGAATTTGTCAGGGGTTATGAACGAAATTCATAAATCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAATTGAATATCCATAAAAAATATATTGGtg                                                                                                                                  >  2:3163191/1‑147 (MQ=255)
                    aGCTCGATTAGACGGCAGAATTTGTCAGGGGTTATGAACGAAATTCATAAATCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGttt                                                                                                                                 >  1:494677/1‑148 (MQ=255)
                    aGCTCGATTAGACGGCAGAATTTGTCAGGGGTTATGAACGAAATTCATAAATCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGttt                                                                                                                                 >  1:770466/1‑148 (MQ=255)
                     gCTCGATTAGACGGCAGAATTTGTCAGGGGTTATGAACGAAATTCATAAATCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATTCATAAAAAATATATTGGTTTa                                                                                                                                <  1:2229460/148‑1 (MQ=255)
                     gCTCGATTAGACGGCAGAATTTGTCAGGGGTTATGAACGAAATTCATAAATCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATTCATAAAAAATATATTGGTTTa                                                                                                                                <  1:3265762/148‑1 (MQ=255)
                     gCTCGATTAGACGGCAGAATTTGTCAGGGGTTATGAACGAAATTCATAAATCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTa                                                                                                                                >  2:432383/1‑148 (MQ=255)
                      cTCGATTAGACGGCAGAATTTGTCAGGGGTTATGAACGAAATTCATAAATCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTAt                                                                                                                               >  2:314273/1‑148 (MQ=255)
                       tCGATTAGACGGCAGAATTTGTCAGGGGTTATGAACGAAATTCATAAATCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATc                                                                                                                              <  2:410451/148‑1 (MQ=255)
                        cGATTAGACGGCAGAATTTGTCAGGGGTTATGAACGAAATTCATAAATCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATcc                                                                                                                             >  1:514245/1‑148 (MQ=255)
                          aTTAGACGGCAGAATTTGTCAGGGGTTATGAACGAAATTCATAAATCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTg                                                                                                                           >  2:1607994/1‑148 (MQ=255)
                           ttAGACGGCAGAATTTGTCAGGGGTTATGAACGAAATTCATAAATCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTgg                                                                                                                          <  1:1004910/148‑1 (MQ=255)
                           ttAGACGGCAGAATTTGTCAGGGGTTATGAACGAAATTCATAAATCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTgg                                                                                                                          <  1:3195731/148‑1 (MQ=255)
                           ttAGACGGCAGAATTTGTCAGGGGTTATGAACGAAATTCATAAATCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTgg                                                                                                                          >  1:1577404/1‑148 (MQ=255)
                            tAGACGGCAGAATTTGTCAGGGGTTATGAACGAAATTCATAAATCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGc                                                                                                                         <  2:1311190/148‑1 (MQ=255)
                            tAGACGGCAGAATTTGTCAGGGGTTATGAACGAAATTCATAAATCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGc                                                                                                                         >  1:3424902/1‑148 (MQ=255)
                                 ggCAGAATTTGTCAGGGGTTATGAACGAAATTCATAAATCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATAAATTGGTTTATCAGGGCatata                                                                                                                    >  1:3041295/1‑148 (MQ=255)
                                    aGAATTTGTCAGGGGTTATGAACGAAATTCATAAATCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATAc                                                                                                                 <  2:13609/148‑1 (MQ=255)
                                    aGAATTTGTCAGGGGTTATGAACGAAATTCATAAATCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATAc                                                                                                                 <  2:207903/148‑1 (MQ=255)
                                     gAATTTGTCAGGGGTTATGAACGAAATTCATAAATCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATAcc                                                                                                                >  2:3398120/1‑148 (MQ=255)
                                      aaTTTGTCAGGGGTTATGAACGAAATTCATAAATCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCt                                                                                                               <  2:1120087/148‑1 (MQ=255)
                                        tttGTCAGGGGTTATGAACGAAATTCATAAATCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATTCATAAAAAATATATTGGTTTATCCTGGCATATATACCTAt                                                                                                             >  2:2215054/1‑148 (MQ=255)
                                        tttGTCAGGGGTTATGAACGAAATTCATAAATCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTAt                                                                                                             >  1:1670258/1‑148 (MQ=255)
                                            tCAGGGGTTATGAACGAAATTCATAAATCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCg                                                                                                         <  2:1471733/148‑1 (MQ=255)
                                               ggggTTATGAACGAAATTCATAAATCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACg                                                                                                      >  2:2385346/1‑148 (MQ=255)
                                                  gTTATGAACGAAATTCATAAATCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTAt                                                                                                   >  2:682473/1‑148 (MQ=255)
                                                  gTTATGAACGAAATTCATAAATCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTAt                                                                                                   >  2:188337/1‑148 (MQ=255)
                                                       gAACGAAATTCATAAATCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCa                                                                                              <  2:1420274/148‑1 (MQ=255)
                                                         aCGAAATTCATAAATCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAAt                                                                                            <  2:1546748/148‑1 (MQ=255)
                                                         aCGAAATTCATAAATCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAAt                                                                                            >  2:1174963/1‑148 (MQ=255)
                                                          cGAAATTCATAAATCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATTCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATa                                                                                           <  2:2486761/148‑1 (MQ=255)
                                                          cGAAATTCATAAATCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATa                                                                                           <  1:1421583/148‑1 (MQ=255)
                                                            aaaTTCATAAATCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGt                                                                                         <  2:3477100/148‑1 (MQ=255)
                                                                tCATAAATCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATTAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTtta                                                                                     <  2:1028294/148‑1 (MQ=255)
                                                                      aTCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTaaa                                                                               >  2:348972/1‑148 (MQ=255)
                                                                       tCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAg                                                                              >  1:1188203/1‑148 (MQ=255)
                                                                       tCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAg                                                                              <  1:2980691/148‑1 (MQ=255)
                                                                       tCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAg                                                                              >  1:689571/1‑148 (MQ=255)
                                                                        cTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAgg                                                                             <  2:3224780/148‑1 (MQ=255)
                                                                        cTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAgg                                                                             >  1:2503541/1‑148 (MQ=255)
                                                                               aGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGTCAGGTCa                                                                      >  1:2416271/1‑148 (MQ=255)
                                                                               aGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCa                                                                      >  1:650033/1‑148 (MQ=255)
                                                                               aGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCa                                                                      >  2:2107912/1‑148 (MQ=255)
                                                                                 tgtTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCAtt                                                                    >  1:3241530/1‑148 (MQ=255)
                                                                                 tgtTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCAtt                                                                    <  2:1670110/148‑1 (MQ=255)
                                                                                       gATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTAtt                                                              <  1:2836015/148‑1 (MQ=255)
                                                                                        aTGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTc                                                             <  2:1202629/148‑1 (MQ=255)
                                                                                          gggTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATAAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCAc                                                           <  1:1929240/148‑1 (MQ=255)
                                                                                                tGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGaa                                                     <  2:2718827/148‑1 (MQ=255)
                                                                                                tGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGa                                                      <  2:1646616/147‑1 (MQ=255)
                                                                                                tGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGa                                                      <  2:932712/147‑1 (MQ=255)
                                                                                                     gTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTg                                                >  2:3302468/1‑148 (MQ=255)
                                                                                                     gTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTg                                                >  1:706456/1‑148 (MQ=255)
                                                                                                      ttCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGa                                               >  1:2669565/1‑148 (MQ=255)
                                                                                                        cGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAat                                             >  1:2165622/1‑148 (MQ=255)
                                                                                                          atatCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAatta                                           <  2:2837866/148‑1 (MQ=255)
                                                                                                          atatCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAatta                                           >  1:972033/1‑148 (MQ=255)
                                                                                                            atCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATTCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAattatt                                         >  1:3516080/1‑148 (MQ=255)
                                                                                                            atCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAattatt                                         <  1:786056/148‑1 (MQ=255)
                                                                                                             tctcCGCAATTTACAGTCCGATGAAGGAAAATGAATATTGATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTc                                        >  1:3296399/1‑148 (MQ=255)
                                                                                                             tctcCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTc                                        <  1:1375675/148‑1 (MQ=255)
                                                                                                             tctcCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTc                                        >  2:2794918/1‑148 (MQ=255)
                                                                                                             tctcCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTc                                        <  1:1375687/148‑1 (MQ=255)
                                                                                                             tctcCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTc                                        >  2:3460693/1‑148 (MQ=255)
                                                                                                                ccGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACt                                     <  2:243030/148‑1 (MQ=255)
                                                                                                                ccGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACt                                     <  1:1961727/148‑1 (MQ=255)
                                                                                                                     aTTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCg                                <  2:3515056/148‑1 (MQ=255)
                                                                                                                            gTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTa                         >  1:1700932/1‑148 (MQ=255)
                                                                                                                             tCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTaa                        >  1:2429148/1‑148 (MQ=255)
                                                                                                                              ccGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAg                       <  2:2687578/148‑1 (MQ=255)
                                                                                                                              ccGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAg                       <  1:849194/148‑1 (MQ=255)
                                                                                                                                  tGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAat                   >  2:1335326/1‑148 (MQ=255)
                                                                                                                                       gAAAATGAATATTCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTCAGAAATATAAc              >  2:2301638/1‑148 (MQ=255)
                                                                                                                                       gAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAAc              <  2:3025419/148‑1 (MQ=255)
                                                                                                                                        aaaaTGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATAAAACt             >  1:2940250/1‑148 (MQ=255)
                                                                                                                                          aaTGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTa           >  1:3352197/1‑148 (MQ=255)
                                                                                                                                            ttaaTATTCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCTGAGTAAGAAATATAACTTAgg         >  1:1569760/3‑148 (MQ=255)
                                                                                                                                              aaTATTCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTa       >  2:1448367/1‑148 (MQ=255)
                                                                                                                                               atatCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTAt      <  1:314273/148‑1 (MQ=255)
                                                                                                                                               atatCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTAt      >  2:2151180/1‑148 (MQ=255)
                                                                                                                                                tatTCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATc     >  1:2481606/1‑148 (MQ=255)
                                                                                                                                                 atCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCt    >  2:1423142/1‑148 (MQ=255)
                                                                                                                                                  tCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTa   >  2:1710078/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AAGCATAACGTAAAGGGAAAAGCTCGATTAGACGGCAGAATTTGTCAGGGGTTATGAACGAAATTCATAAATCTGTTTGAGTGTTGCGATGGGTAGTGCAAGTTCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTAT  >  CP009273/309533‑309827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑