Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,782,439 G→T 8.8% R335S (CGC→AGC)  waaH ← LPS(HepIII)‑glucuronic acid glycosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,782,4390GT 8.8% 868.8 / 34.2 247R335S (CGC→AGC) waaHLPS(HepIII)‑glucuronic acid glycosyltransferase
Reads supporting (aligned to +/- strand):  ref base G (119/105);  new base T (9/14);  total (128/119)
Fisher's exact test for biased strand distribution p-value = 2.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 248 total aligned reads displayed.

GCTAATCGCTTCTTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATATCTCGGCAATCATCCGTTGGCGGG  >  CP009273/3782292‑3782585
                                                                                                                                                   |                                                                                                                                                  
gCTAATCGCTTCTTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCg                                                                                                                                                    <  2:1760794/148‑1 (MQ=255)
 cTAATCGCTTCTTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATGAAAGGCGCTACGCGTAATGCGa                                                                                                                                                   <  2:510453/148‑1 (MQ=255)
  tAATCGCTTCTTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGAt                                                                                                                                                  <  2:2217269/148‑1 (MQ=255)
   aaTCGCTTCTTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATg                                                                                                                                                 <  2:570308/148‑1 (MQ=255)
    aTCGCTTCTTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATgg                                                                                                                                                >  2:484489/1‑148 (MQ=255)
         ttcttTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGa                                                                                                                                           >  1:2499469/1‑148 (MQ=255)
              tGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCTTAATGCGATGGTGCGACcgcg                                                                                                                                      <  1:3552159/148‑1 (MQ=255)
               gAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGt                                                                                                                                     >  1:2551433/1‑148 (MQ=255)
               gAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGt                                                                                                                                     >  1:1593614/1‑148 (MQ=255)
                aaGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGtt                                                                                                                                    <  2:914892/148‑1 (MQ=255)
                    aTTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCTATGGTGCGACCGCGTTTTGt                                                                                                                                <  2:2037198/148‑1 (MQ=255)
                    aTTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCTATGGTGCGACCGCGTTTTGt                                                                                                                                <  2:2037886/148‑1 (MQ=255)
                      tAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTcg                                                                                                                              >  1:2454263/1‑148 (MQ=255)
                         gCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCgcgc                                                                                                                           >  1:2222130/1‑148 (MQ=255)
                         gCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCgcgc                                                                                                                           >  1:2989837/1‑148 (MQ=255)
                         gCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCgcgc                                                                                                                           >  2:2250151/1‑148 (MQ=255)
                          cGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGcc                                                                                                                          <  1:2777294/148‑1 (MQ=255)
                           gAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCa                                                                                                                         <  1:1905526/148‑1 (MQ=255)
                              aGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACTCTTAATGCGATGGTGAGACCGCGTTTTGTCGCGCCATTg                                                                                                                      >  1:548948/1‑148 (MQ=255)
                              aGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTg                                                                                                                      >  1:2337362/1‑148 (MQ=255)
                              aGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTg                                                                                                                      >  1:282106/1‑148 (MQ=255)
                                cATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGcc                                                                                                                    <  1:2059235/148‑1 (MQ=255)
                                     gCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGATGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAg                                                                                                               >  2:727538/1‑148 (MQ=255)
                                       cAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTANCGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCg                                                                                                             >  1:1807488/1‑148 (MQ=255)
                                          agCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGaa                                                                                                          >  1:759734/1‑148 (MQ=255)
                                            cACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAg                                                                                                        <  2:1252806/148‑1 (MQ=255)
                                            cACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAg                                                                                                        <  1:988486/148‑1 (MQ=255)
                                            cACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAg                                                                                                        >  2:738508/1‑148 (MQ=255)
                                              cGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGAc                                                                                                      <  1:2650239/148‑1 (MQ=255)
                                              cGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGAc                                                                                                      >  1:102609/1‑148 (MQ=255)
                                              cGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGAc                                                                                                      >  1:2440108/1‑148 (MQ=255)
                                                 tGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACcac                                                                                                   >  1:916104/1‑148 (MQ=255)
                                                  gATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACcaca                                                                                                  <  2:2857613/148‑1 (MQ=255)
                                                   aTCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAg                                                                                                 >  1:858750/1‑148 (MQ=255)
                                                       tttACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAAc                                                                                             >  1:1928486/1‑148 (MQ=255)
                                                        ttACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACg                                                                                            >  2:66466/1‑148 (MQ=255)
                                                        ttACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACg                                                                                            >  2:3531110/1‑148 (MQ=255)
                                                          aCTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGcc                                                                                          <  1:1378408/148‑1 (MQ=255)
                                                             tCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTgg                                                                                       >  1:2839501/1‑148 (MQ=255)
                                                             tCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTgg                                                                                       <  2:2134351/148‑1 (MQ=255)
                                                                  cATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAAcc                                                                                  >  1:1929177/1‑148 (MQ=255)
                                                                    tctgTGGCAAGGCTGGGGAAAACAGCCCGATGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGATACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCTTTGCCATAAGCGTAAAGACCACAGTAACGCCTGGTAACCGa                                                                                <  1:57729/145‑1 (MQ=255)
                                                                    tCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCACATTAAAGGCGCTACGCGTAATGCTATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGACAGACCACAGTACCGCCTGGTAACCGa                                                                                >  2:1522685/1‑148 (MQ=255)
                                                                    tCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGATACGCGTAATGCGATGGTGCGACCGCTTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGa                                                                                <  1:58227/148‑1 (MQ=255)
                                                                     cAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCTATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGAc                                                                               <  2:2500783/148‑1 (MQ=255)
                                                                     cAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGAc                                                                               <  1:3510282/148‑1 (MQ=255)
                                                                      aggggCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCTTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCAATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGAcc                                                                              <  1:965057/145‑1 (MQ=255)
                                                                            aaGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACg                                                                        <  1:2006423/148‑1 (MQ=255)
                                                                              ggCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCt                                                                      >  2:3155695/1‑148 (MQ=255)
                                                                              ggCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCt                                                                      >  2:2007082/1‑148 (MQ=255)
                                                                                   gggCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGca                                                                 >  1:140964/1‑148 (MQ=255)
                                                                                    ggCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCTATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGcac                                                                <  1:2769504/148‑1 (MQ=255)
                                                                                    ggCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGcac                                                                <  1:2427027/148‑1 (MQ=255)
                                                                                       aaaCAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACAtt                                                             >  2:3349231/1‑148 (MQ=255)
                                                                                        aaCAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAACTTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTg                                                            <  2:1817848/148‑1 (MQ=255)
                                                                                        aaCAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCTATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTg                                                            >  1:2406540/1‑148 (MQ=255)
                                                                                          cAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGt                                                          <  2:665124/148‑1 (MQ=255)
                                                                                          cAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGt                                                          >  1:1062169/1‑148 (MQ=255)
                                                                                             cccGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCTATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAAt                                                       <  1:2949897/148‑1 (MQ=255)
                                                                                             cccGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAAt                                                       >  1:1049092/1‑148 (MQ=255)
                                                                                              ccGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTACTc                                                      >  2:1923778/1‑148 (MQ=255)
                                                                                                gAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAg                                                    <  2:2989837/148‑1 (MQ=255)
                                                                                                gAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAg                                                    >  1:1933170/1‑148 (MQ=255)
                                                                                                gAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAg                                                    >  1:2694260/1‑148 (MQ=255)
                                                                                                gAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAg                                                    >  1:918805/1‑148 (MQ=255)
                                                                                                 aaGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAgg                                                   <  2:573249/148‑1 (MQ=255)
                                                                                                   gcggcgAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGgcg                                                 >  2:2468938/1‑148 (MQ=255)
                                                                                                          aGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTatac                                          >  2:272364/1‑148 (MQ=255)
                                                                                                           gTTTAAGGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCTATGGTGCTACCGCTTTTTGTCTCGCCATTGCCATACGCGGAAAGACCACTGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTataca                                         <  1:378441/148‑1 (MQ=255)
                                                                                                             ttAACGCTAGCGCAAATTAACGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTatacata                                       <  1:2157719/148‑1 (MQ=255)
                                                                                                                    tAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAg                                <  2:1358587/148‑1 (MQ=255)
                                                                                                                      gcgcAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTa                              <  2:140964/148‑1 (MQ=255)
                                                                                                                       cgcAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTaa                             >  2:1960293/1‑148 (MQ=255)
                                                                                                                       cgcAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTaa                             >  2:1285137/1‑148 (MQ=255)
                                                                                                                        gcAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTaaa                            <  1:738184/148‑1 (MQ=255)
                                                                                                                           aaTTAAAGGCGCTACGCGTAATGCTATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAatat                         >  2:3079858/1‑148 (MQ=255)
                                                                                                                            aTTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATAtc                        >  2:2803949/1‑148 (MQ=255)
                                                                                                                            aTTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATAtc                        <  1:3309125/148‑1 (MQ=255)
                                                                                                                            aTTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATAtc                        >  2:2394809/1‑148 (MQ=255)
                                                                                                                              tAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATAtctc                      >  2:2357482/1‑148 (MQ=255)
                                                                                                                              tAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATAtctc                      >  1:727307/1‑148 (MQ=255)
                                                                                                                                aaGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATATCTCgg                    >  1:3151198/1‑148 (MQ=255)
                                                                                                                                 aGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATATCTCGGc                   >  1:201072/1‑148 (MQ=255)
                                                                                                                                      cTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATATCTCGGCAatca              <  2:2658011/148‑1 (MQ=255)
                                                                                                                                      cTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATATCTCGGCAatca              >  1:1245391/1‑148 (MQ=255)
                                                                                                                                      cTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATATCTCGGCAatca              >  1:208868/1‑148 (MQ=255)
                                                                                                                                       tACGCGTAATGCTATGTTGCCCCCGCGTTTTGCCGCGCCATTGCCATACGCGGAAAGCCCACATTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATATCTCGGCAatcat             <  1:1522685/148‑1 (MQ=255)
                                                                                                                                       tACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATATCTCGGCAatcat             <  2:937007/148‑1 (MQ=255)
                                                                                                                                        aCGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATATCTCGGCAatcatc            <  2:109768/148‑1 (MQ=255)
                                                                                                                                        aCGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATATCTCGGCAatcatc            >  2:327615/1‑148 (MQ=255)
                                                                                                                                          gcgTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATATCTCGGCAATCATCCg          <  1:1326066/148‑1 (MQ=255)
                                                                                                                                           cgTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATATCTCGGCAATCATCCGt         <  2:3383045/148‑1 (MQ=255)
                                                                                                                                               aTGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATATCTCGGCAATCATCCGTTGGc     <  2:1134810/148‑1 (MQ=255)
                                                                                                                                                tGCTATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATATCTCGGCAATCATCCGTTGGCg    >  2:3463820/1‑148 (MQ=255)
                                                                                                                                                tGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATATCTCGGCAATCATCCGTTGGCg    >  1:1845491/1‑148 (MQ=255)
                                                                                                                                                tGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATATCTCGGCAATCATCCGTTGGCg    <  2:1593614/148‑1 (MQ=255)
                                                                                                                                                tGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATATCTCGGCAATCATCCGTTGGCg    <  2:2551433/148‑1 (MQ=255)
                                                                                                                                                 gCGATTGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATATCTCGGCAATCATCCGTTGGCgg   >  1:183632/1‑148 (MQ=255)
                                                                                                                                                  cGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATATCTCGGCAATCATCCGTTGGCggg  <  1:1759315/148‑1 (MQ=255)
                                                                                                                                                  cGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATATCTCGGCAATCATCCGTTGGCggg  <  1:422978/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCTAATCGCTTCTTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATATCTCGGCAATCATCCGTTGGCGGG  >  CP009273/3782292‑3782585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑