Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,791,063 T→C 7.2% A252A (GCT→GCC)  waaL → O‑antigen ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,791,0630TC 7.2% 699.0 / 34.7 223A252A (GCT→GCC) waaLO‑antigen ligase
Reads supporting (aligned to +/- strand):  ref base T (92/115);  new base C (5/11);  total (97/126)
Fisher's exact test for biased strand distribution p-value = 4.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 246 total aligned reads displayed.

CGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGCAATGTACGAAATTGGTTTAAATATATTCATAAAG  >  CP009273/3790916‑3791210
                                                                                                                                                   |                                                                                                                                                   
cgcgGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATtagct                                                                                                                                                     >  1:3088322/1‑148 (MQ=255)
 gcgGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATtagcta                                                                                                                                                    <  1:2185655/148‑1 (MQ=255)
   ggTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATtagctagc                                                                                                                                                  <  1:2753165/148‑1 (MQ=255)
    gTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCa                                                                                                                                                 <  2:1703723/148‑1 (MQ=255)
    gTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCa                                                                                                                                                 <  1:1708072/148‑1 (MQ=255)
     tACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCAt                                                                                                                                                <  2:551342/148‑1 (MQ=255)
      aCTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCAtt                                                                                                                                               <  1:339/148‑1 (MQ=255)
        tttATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGt                                                                                                                                             >  2:1046311/1‑148 (MQ=255)
            tGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGttatt                                                                                                                                         >  2:695299/1‑148 (MQ=255)
             gTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGttatta                                                                                                                                        <  1:1519433/148‑1 (MQ=255)
             gTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGttatta                                                                                                                                        <  1:3196846/148‑1 (MQ=255)
              ttCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTtattat                                                                                                                                       <  2:1096246/148‑1 (MQ=255)
              ttCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTtattat                                                                                                                                       <  2:1095458/148‑1 (MQ=255)
               tCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATtata                                                                                                                                      <  1:526873/148‑1 (MQ=255)
               tCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATtata                                                                                                                                      <  2:2184884/148‑1 (MQ=255)
               tCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATtata                                                                                                                                      <  1:2089136/148‑1 (MQ=255)
                cTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTatat                                                                                                                                     <  2:2939900/148‑1 (MQ=255)
                    cgcTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTtaa                                                                                                                                 >  2:524013/1‑148 (MQ=255)
                     gcTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTtaat                                                                                                                                >  2:2356417/1‑148 (MQ=255)
                       tAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTtaataa                                                                                                                              >  1:328613/1‑148 (MQ=255)
                         acacAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAAc                                                                                                                            <  1:1083684/148‑1 (MQ=255)
                          cacaAACCAGAGCAACCCTACTCCTGTTCACTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAACAAATTCACTTCCTCTATTGTTCTACTACTTGCTAGATTAGCTCGCATGGTTATTAGAGTTAATATAcc                                                                                                                           >  1:1956424/1‑148 (MQ=255)
                           acaAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCa                                                                                                                          <  2:1037406/148‑1 (MQ=255)
                           acaAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCCAGCATTGTTATTATATTTAATAAACCa                                                                                                                          <  2:1250814/148‑1 (MQ=255)
                             aaaCCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAAt                                                                                                                        <  2:1054136/148‑1 (MQ=255)
                             aaaCCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAAt                                                                                                                        >  1:2079153/1‑148 (MQ=255)
                              aaCCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATa                                                                                                                       <  2:3539322/148‑1 (MQ=255)
                                ccAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACa                                                                                                                     >  1:2253367/1‑148 (MQ=255)
                                 cAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAg                                                                                                                    >  2:3222267/1‑148 (MQ=255)
                                 cAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAg                                                                                                                    <  2:2874756/148‑1 (MQ=255)
                                  agagCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGa                                                                                                                   <  1:491122/148‑1 (MQ=255)
                                   gagCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGaa                                                                                                                  >  2:3378451/1‑148 (MQ=255)
                                   gagCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGaa                                                                                                                  >  1:1343536/1‑148 (MQ=255)
                                    agCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAAt                                                                                                                 >  2:3045939/1‑148 (MQ=255)
                                    agCAACACTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGATATCATTGTTATTATATTTAATAAAACAATAAAGAAt                                                                                                                 >  2:2033245/1‑148 (MQ=255)
                                        aCCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCt                                                                                                             <  1:396014/148‑1 (MQ=255)
                                        aCCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCt                                                                                                             <  1:51641/148‑1 (MQ=255)
                                        aCCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCt                                                                                                             <  1:587057/148‑1 (MQ=255)
                                         cccTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCta                                                                                                            <  2:1987945/148‑1 (MQ=255)
                                          ccTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCtat                                                                                                           >  1:3214804/1‑148 (MQ=255)
                                           cTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCtata                                                                                                          <  2:584109/148‑1 (MQ=255)
                                             aCTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAAt                                                                                                        >  1:3327341/1‑148 (MQ=255)
                                              cTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATg                                                                                                       <  2:2396364/148‑1 (MQ=255)
                                                ccTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGaa                                                                                                     <  2:2896646/148‑1 (MQ=255)
                                                 cTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAg                                                                                                    <  2:351741/148‑1 (MQ=255)
                                                 cTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAg                                                                                                    >  2:1354397/1‑148 (MQ=255)
                                                 cTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGATATAATGAAg                                                                                                    >  1:2977732/1‑148 (MQ=255)
                                                  tGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGc                                                                                                   <  2:260009/148‑1 (MQ=255)
                                                   gTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCa                                                                                                  <  2:1519406/148‑1 (MQ=255)
                                                       ccTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATGATGAAGCATTaa                                                                                              <  1:129954/148‑1 (MQ=255)
                                                         tataATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAAt                                                                                            >  2:3115992/1‑148 (MQ=255)
                                                         tataATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAAt                                                                                            <  2:3356134/148‑1 (MQ=255)
                                                           taATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGa                                                                                          <  1:2304028/148‑1 (MQ=255)
                                                                tgtgTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTaa                                                                                     <  2:1865069/148‑1 (MQ=255)
                                                                   gtTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACa                                                                                  >  2:820887/1‑148 (MQ=255)
                                                                   gtTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACa                                                                                  <  1:1314569/148‑1 (MQ=255)
                                                                    ttGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAg                                                                                 >  2:2754715/1‑148 (MQ=255)
                                                                    ttGCGGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAg                                                                                 >  1:2600928/1‑148 (MQ=255)
                                                                      gctgcATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGtt                                                                               <  1:1064231/148‑1 (MQ=255)
                                                                       ctgcATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTta                                                                              >  1:1031202/1‑148 (MQ=255)
                                                                        tgcATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTtat                                                                             >  1:1630692/1‑148 (MQ=255)
                                                                         gcATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTtata                                                                            <  2:1834319/148‑1 (MQ=255)
                                                                              aaTAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAAt                                                                       >  2:2614362/1‑148 (MQ=255)
                                                                               aTAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATg                                                                      <  2:3015302/148‑1 (MQ=255)
                                                                               aTAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCCAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATg                                                                      <  1:2589423/148‑1 (MQ=255)
                                                                                 aGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCt                                                                    <  1:19156/148‑1 (MQ=255)
                                                                                  gCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTa                                                                   >  1:2504297/1‑148 (MQ=255)
                                                                                   cTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTaa                                                                  <  2:718941/148‑1 (MQ=255)
                                                                                          taataaATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGtt                                                           >  2:1182174/1‑148 (MQ=255)
                                                                                              aaaTCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACtt                                                       >  2:2642665/1‑148 (MQ=255)
                                                                                                 tCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTccc                                                    >  1:2973003/1‑148 (MQ=255)
                                                                                                     ccAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAgg                                                <  2:1325256/148‑1 (MQ=255)
                                                                                                            aTTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCCAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGa                                         >  1:2559272/1‑148 (MQ=255)
                                                                                                              tCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACt                                       >  2:525939/1‑148 (MQ=255)
                                                                                                               cACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTg                                      <  1:3045869/148‑1 (MQ=255)
                                                                                                                       ctATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGCAATGTa                              <  1:1182174/148‑1 (MQ=255)
                                                                                                                           tGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGCAATGTACGaa                          <  1:3366387/148‑1 (MQ=255)
                                                                                                                            gTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGCAATGTACGaaa                         >  1:3141123/1‑148 (MQ=255)
                                                                                                                            gTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGCAATGTACGaaa                         <  1:721311/148‑1 (MQ=255)
                                                                                                                            gTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGCAATGTACGaaa                         <  1:2505756/148‑1 (MQ=255)
                                                                                                                            gTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGCAATGTACGaaa                         <  1:1973070/148‑1 (MQ=255)
                                                                                                                             ttCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGCAATGTACGAAAt                        <  1:695299/148‑1 (MQ=255)
                                                                                                                             ttCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGCAATGTACGAAAt                        <  1:1942100/148‑1 (MQ=255)
                                                                                                                              tCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGCAATGTACGAAAtt                       >  2:1288169/1‑148 (MQ=255)
                                                                                                                                tactaATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGCAATGTACGAAATTgg                     <  1:952718/148‑1 (MQ=255)
                                                                                                                                tactaATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGCAATGTACGAAATTgg                     >  2:3465798/1‑148 (MQ=255)
                                                                                                                                tactaATTGCTATATTAGCCAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGCAATGTACGAAATTgg                     <  1:280733/148‑1 (MQ=255)
                                                                                                                                 actaATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGCAATGTACGAAATTGGt                    <  1:2736147/148‑1 (MQ=255)
                                                                                                                                 actaATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGCAATGTACGAAATTGGt                    <  2:792383/148‑1 (MQ=255)
                                                                                                                                     aTTGCTATATTAGCCAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGCAATGTACGAAATTGGTTTaa                <  1:1571890/148‑1 (MQ=255)
                                                                                                                                       tGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGCAATGTACGAAATTGGTTTAAat              >  2:2228841/1‑148 (MQ=255)
                                                                                                                                        gCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGCAATGTACGAAATTGGTTTAAata             >  2:327499/1‑148 (MQ=255)
                                                                                                                                        gCTATATTAGCCAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGCAATGTACGAAATTGGTTTAAata             <  1:262368/148‑1 (MQ=255)
                                                                                                                                         cTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGCAATGTACGAAATTGGTTTAAatat            <  2:2824178/148‑1 (MQ=255)
                                                                                                                                             atTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGCAATGTACGAAATTGGTTTAAATATATTc        >  1:734053/1‑148 (MQ=255)
                                                                                                                                                 gctagcATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGCAATGTACGAAATTGGTTTAAATATATTCATaa    >  1:2322230/1‑148 (MQ=255)
                                                                                                                                                  ctagcATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGCAATGTACGAAATTGGTTTAAATATATTCATaaa   >  1:1691458/1‑148 (MQ=255)
                                                                                                                                                  ctagcATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCGAGGTGCAAGACTGGCAATGTACGAAATTGGTTTAAATATATTAATaaa   >  1:3444361/1‑148 (MQ=255)
                                                                                                                                                   tagcATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGCAATGTACGAAATTGGTTTAAATATATTCATAAAg  <  1:2465859/148‑1 (MQ=255)
                                                                                                                                                   tagcATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGCAATGTACGAAATTGGTTTAAATATATTCATAAAg  >  1:2866726/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGCAATGTACGAAATTGGTTTAAATATATTCATAAAG  >  CP009273/3790916‑3791210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑