Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,828,206 T→A 100% S362C (AGT→TGT)  yicJ ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,828,2060TA97.7% 565.9 / 2.3 173S362C (AGT→TGT) yicJputative transporter
Reads supporting (aligned to +/- strand):  ref base T (2/2);  new base A (76/93);  total (78/95)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 173 total aligned reads displayed.

GAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACTGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGGCCTGCATGG  >  CP009273/3828063‑3828349
                                                                                                                                               |                                                                                                                                               
gAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATc                                                                                                                                             <  2:752526/148‑1 (MQ=255)
 aaTAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATcc                                                                                                                                            <  2:2745787/148‑1 (MQ=255)
 aaTAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATcc                                                                                                                                            <  2:2137358/148‑1 (MQ=255)
 aaTAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATcc                                                                                                                                            <  2:2137518/148‑1 (MQ=255)
 aaTAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATcc                                                                                                                                            <  1:841444/148‑1 (MQ=255)
   tAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCg                                                                                                                                          <  1:2084822/148‑1 (MQ=255)
   tAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCg                                                                                                                                          <  1:68162/148‑1 (MQ=255)
   tAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCg                                                                                                                                          <  1:2876554/148‑1 (MQ=255)
   tAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCg                                                                                                                                          <  1:1024690/148‑1 (MQ=255)
    aGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCgg                                                                                                                                         >  1:2163349/1‑148 (MQ=255)
    aGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCgg                                                                                                                                         >  1:317277/1‑148 (MQ=255)
      cgcAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTc                                                                                                                                       <  2:591588/148‑1 (MQ=255)
         aataatGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAgg                                                                                                                                    <  1:1686677/148‑1 (MQ=255)
          ataatGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGc                                                                                                                                   >  2:2367827/1‑148 (MQ=255)
          ataatGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGc                                                                                                                                   >  2:3552175/1‑148 (MQ=255)
            aatGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGt                                                                                                                                 >  1:2189863/1‑148 (MQ=255)
            aatGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGt                                                                                                                                 >  1:476532/1‑148 (MQ=255)
             atgatgCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGtt                                                                                                                                <  2:3442239/148‑1 (MQ=255)
             atgatgCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGtt                                                                                                                                <  1:1344120/148‑1 (MQ=255)
             atgatgCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGtt                                                                                                                                >  2:1201033/1‑148 (MQ=255)
              tgatgCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGttt                                                                                                                               <  2:326822/148‑1 (MQ=255)
                atgCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTAc                                                                                                                             <  1:1134408/148‑1 (MQ=255)
                 tgCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCg                                                                                                                                  >  1:1782008/1‑142 (MQ=255)
                 tgCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCg                                                                                                                                  <  2:1782008/142‑1 (MQ=255)
                   cTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCAt                                                                                                                          <  1:762604/148‑1 (MQ=255)
                          tGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGcacca                                                                                                                   <  1:1031280/148‑1 (MQ=255)
                          tGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGcacca                                                                                                                   >  1:3314822/1‑148 (MQ=255)
                          tGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGcacca                                                                                                                   <  1:1336836/148‑1 (MQ=255)
                           ggCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGcaccac                                                                                                                  <  2:1581032/148‑1 (MQ=255)
                           ggCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGcaccac                                                                                                                  <  2:1720493/148‑1 (MQ=255)
                            gcgcTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACt                                                                                                                 <  2:3453644/148‑1 (MQ=255)
                               cTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGc                                                                                                              <  1:2990680/148‑1 (MQ=255)
                                tGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATCAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGcc                                                                                                             <  2:286049/148‑1 (MQ=255)
                                tGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGcc                                                                                                             <  2:2081077/148‑1 (MQ=255)
                                 ggtCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCg                                                                                                            <  2:372030/146‑1 (MQ=255)
                                 gTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCg                                                                                                            >  2:2196012/1‑148 (MQ=255)
                                 gTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCg                                                                                                            >  2:1274470/1‑148 (MQ=255)
                                  ttCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGt                                                                                                           >  2:2458411/1‑148 (MQ=255)
                                       cgcTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCg                                                                                                      >  2:910289/1‑148 (MQ=255)
                                         cTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGAc                                                                                                    >  1:1843981/1‑148 (MQ=255)
                                          tttttCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACg                                                                                                   <  2:3314822/148‑1 (MQ=255)
                                          tttttCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACg                                                                                                   >  2:2355338/1‑148 (MQ=255)
                                          tttttCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACg                                                                                                   >  1:63302/1‑148 (MQ=255)
                                          tttttCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACg                                                                                                   <  2:467469/148‑1 (MQ=255)
                                            tttCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGt                                                                                                 <  2:3009707/148‑1 (MQ=255)
                                            tttCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGt                                                                                                 <  2:3009611/148‑1 (MQ=255)
                                              tCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTAt                                                                                               <  2:1508689/148‑1 (MQ=255)
                                                     catcatATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGAcat                                                                                        <  2:976131/148‑1 (MQ=255)
                                                      atcatATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGAcatc                                                                                       <  2:487911/148‑1 (MQ=255)
                                                      atcatATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGAcatc                                                                                       <  2:1687644/148‑1 (MQ=255)
                                                       tcatATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGAcatca                                                                                      >  2:505656/1‑148 (MQ=255)
                                                        catATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGAcatcat                                                                                     >  2:2542461/1‑148 (MQ=255)
                                                         atatCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCAtt                                                                                    <  1:1297028/148‑1 (MQ=255)
                                                           atCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTAc                                                                                  >  1:133295/1‑148 (MQ=255)
                                                             ccGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTAccc                                                                                >  2:3437776/1‑148 (MQ=255)
                                                              cGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCa                                                                               >  2:1462910/1‑148 (MQ=255)
                                                                   tAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGa                                                                          >  2:2782055/1‑148 (MQ=255)
                                                                    aaGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGAt                                                                         <  2:1780846/148‑1 (MQ=255)
                                                                     aGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATa                                                                        >  2:423433/1‑148 (MQ=255)
                                                                      gCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAg                                                                       >  1:2457704/1‑148 (MQ=255)
                                                                        cAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGgt                                                                     <  2:106373/148‑1 (MQ=255)
                                                                                 gCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGt                                                            <  1:1267820/148‑1 (MQ=255)
                                                                                  ccGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCTCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGtt                                                           >  1:3554306/1‑148 (MQ=255)
                                                                                  ccGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCTCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGtt                                                           >  1:1598058/1‑148 (MQ=255)
                                                                                   cGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTg                                                          >  2:873663/1‑148 (MQ=255)
                                                                                     aTAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGAt                                                        >  1:3354568/1‑148 (MQ=255)
                                                                                      tAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATg                                                       >  2:2146629/1‑148 (MQ=255)
                                                                                      tAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATg                                                       >  2:2012916/1‑148 (MQ=255)
                                                                                         gagCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCaa                                                    <  1:1599766/148‑1 (MQ=255)
                                                                                            cgccgcCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAacac                                                 <  1:1244661/148‑1 (MQ=255)
                                                                                            cgccgcCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAacac                                                 <  1:942439/148‑1 (MQ=255)
                                                                                            cgccgcCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAacac                                                 >  1:2057209/1‑148 (MQ=255)
                                                                                                   gAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATc                                          <  1:3027452/148‑1 (MQ=255)
                                                                                                      ggCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACg                                       >  2:3126386/1‑148 (MQ=255)
                                                                                                      ggCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCAATACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACg                                       >  2:927373/1‑148 (MQ=255)
                                                                                                       gCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACTGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGa                                      >  2:1582804/1‑148 (MQ=255)
                                                                                                        ccAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGaa                                     >  1:3443887/1‑148 (MQ=255)
                                                                                                         cAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAg                                    <  2:317277/148‑1 (MQ=255)
                                                                                                            ccccAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATg                                 <  1:354527/148‑1 (MQ=255)
                                                                                                             cccAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGa                                <  1:3271545/148‑1 (MQ=255)
                                                                                                             cccAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGa                                >  2:3434178/1‑148 (MQ=255)
                                                                                                             cccAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGa                                <  2:2457704/148‑1 (MQ=255)
                                                                                                             cccAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGa                                <  1:1697465/148‑1 (MQ=255)
                                                                                                             cccAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGa                                <  2:2048272/148‑1 (MQ=255)
                                                                                                              ccAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCATCACACCAATCACGAAGATGaa                               <  2:476555/148‑1 (MQ=255)
                                                                                                              ccAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGaa                               <  1:550050/148‑1 (MQ=255)
                                                                                                                 gTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGAc                            <  1:498566/148‑1 (MQ=255)
                                                                                                                       gCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACAt                      >  2:1963780/1‑148 (MQ=255)
                                                                                                                         acaAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAg                    <  2:1907186/148‑1 (MQ=255)
                                                                                                                           aaaCAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTg                  <  1:3126386/148‑1 (MQ=255)
                                                                                                                           aaaCAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTg                  <  1:2311551/148‑1 (MQ=255)
                                                                                                                               aGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGc              <  1:1496230/148‑1 (MQ=255)
                                                                                                                               aGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGc              <  1:742848/148‑1 (MQ=255)
                                                                                                                               aGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGc              <  2:1471421/148‑1 (MQ=255)
                                                                                                                                 cGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTg            >  2:226423/1‑148 (MQ=255)
                                                                                                                                 cGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTg            >  2:226357/1‑148 (MQ=255)
                                                                                                                                     ccAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATTCTGGCCt        >  1:2701465/1‑148 (MQ=255)
                                                                                                                                         cAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGGCCTGCAt    <  2:1002209/148‑1 (MQ=255)
                                                                                                                                         cAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGGCCTGCAt    <  1:2615567/148‑1 (MQ=255)
                                                                                                                                           aaaCTGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGGCCTGCATgg  <  2:2575465/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                               
GAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACTGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGGCCTGCATGG  >  CP009273/3828063‑3828349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑