Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,830,819 T→G 100% F169L (TTT→TTG)  setC → putative arabinose efflux transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,830,8190TG96.9% 880.3 / 4.4 222F169L (TTT→TTG) setCputative arabinose efflux transporter
Reads supporting (aligned to +/- strand):  ref base T (2/5);  new base G (94/121);  total (96/126)
Fisher's exact test for biased strand distribution p-value = 7.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 222 total aligned reads displayed.

CAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTTAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTCCACCCACAGGAAGCGG  >  CP009273/3830674‑3830963
                                                                                                                                                 |                                                                                                                                                
cAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAg                                                                                                                                                <  2:459146/148‑1 (MQ=255)
  aaCCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGtt                                                                                                                                              <  2:57462/148‑1 (MQ=255)
    cccGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGtttt                                                                                                                                            <  1:2362341/148‑1 (MQ=255)
       gCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTaaa                                                                                                                                         >  2:2173478/1‑148 (MQ=255)
        cAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAg                                                                                                                                        >  1:2534400/1‑148 (MQ=255)
         aaaTGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGTCGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGt                                                                                                                                       <  1:207726/148‑1 (MQ=255)
         aaaTGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTATACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGt                                                                                                                                       <  1:443967/148‑1 (MQ=255)
          aaTGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTg                                                                                                                                      <  1:1285944/148‑1 (MQ=255)
          aaTGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTg                                                                                                                                      <  1:493681/148‑1 (MQ=255)
          aaTGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTg                                                                                                                                      <  1:2415507/148‑1 (MQ=255)
              ttCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGt                                                                                                                                  <  2:2534344/148‑1 (MQ=255)
               tcgccctcgcccGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTa                                                                                                                                 >  2:440836/1‑148 (MQ=255)
               tcgccctcgcccGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTa                                                                                                                                 <  1:2844059/148‑1 (MQ=255)
                cgccctcgcccGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTAt                                                                                                                                <  1:658570/148‑1 (MQ=255)
                    ctcgcccGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCa                                                                                                                            <  2:2215580/148‑1 (MQ=255)
                       gcccGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCg                                                                                                                         <  2:3139115/148‑1 (MQ=255)
                       gcccGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCg                                                                                                                         <  2:1562914/148‑1 (MQ=255)
                          cGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTg                                                                                                                      <  1:1894800/148‑1 (MQ=255)
                           gTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGc                                                                                                                     >  1:3310126/1‑148 (MQ=255)
                           gTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGc                                                                                                                     >  1:2227366/1‑148 (MQ=255)
                            tGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTNGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGcc                                                                                                                    <  1:1805632/148‑1 (MQ=255)
                            tGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGcc                                                                                                                    >  2:3555029/1‑148 (MQ=255)
                             gAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCa                                                                                                                   <  1:2979812/148‑1 (MQ=255)
                             gAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCa                                                                                                                   <  2:2119020/148‑1 (MQ=255)
                             gAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCa                                                                                                                   >  2:1830315/1‑148 (MQ=255)
                              aaCACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCAt                                                                                                                  <  1:275533/148‑1 (MQ=255)
                              aaCACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCAt                                                                                                                  <  2:1700981/148‑1 (MQ=255)
                               acacGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATc                                                                                                                 >  2:128157/1‑148 (MQ=255)
                                cacGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCCCCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCg                                                                                                                >  2:663012/1‑148 (MQ=255)
                                  cGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACGTTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTTAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCa                                                                                                              <  2:1197978/148‑1 (MQ=255)
                                  cGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCa                                                                                                              <  2:1507152/148‑1 (MQ=255)
                                   gCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCAt                                                                                                             <  1:594177/148‑1 (MQ=255)
                                   gCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCAt                                                                                                             <  2:806801/148‑1 (MQ=255)
                                   gCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCAt                                                                                                             <  1:3525484/148‑1 (MQ=255)
                                   gCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCAt                                                                                                             <  2:281409/148‑1 (MQ=255)
                                    ccGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCAtt                                                                                                            <  1:2897007/148‑1 (MQ=255)
                                    ccGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCAtt                                                                                                            >  1:1499912/1‑148 (MQ=255)
                                          gAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTtgttgt                                                                                                      <  2:1467763/148‑1 (MQ=255)
                                                gCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCgg                                                                                                <  1:3232037/148‑1 (MQ=255)
                                                    tGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTg                                                                                            <  1:2426112/148‑1 (MQ=255)
                                                      agaCGGTCATGTTCAATACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGAt                                                                                          <  1:3267841/148‑1 (MQ=255)
                                                         cGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGt                                                                                       <  1:664709/148‑1 (MQ=255)
                                                         cGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGt                                                                                       <  1:2693435/148‑1 (MQ=255)
                                                             cATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTgg                                                                                   >  1:705182/1‑148 (MQ=255)
                                                                gTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGgttg                                                                                <  1:1698589/148‑1 (MQ=255)
                                                                 ttCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGgttgt                                                                               <  2:2586783/148‑1 (MQ=255)
                                                                 ttCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGgttgt                                                                               <  1:445894/148‑1 (MQ=255)
                                                                 ttCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGgttgt                                                                               <  2:2709248/148‑1 (MQ=255)
                                                                     gTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGttttt                                                                           <  2:2539403/148‑1 (MQ=255)
                                                                     gTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGttttt                                                                           >  1:2613280/1‑148 (MQ=255)
                                                                      tACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTg                                                                          >  1:15558/1‑148 (MQ=255)
                                                                      tACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTg                                                                          >  1:15426/1‑148 (MQ=255)
                                                                       aCATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGc                                                                         >  1:1142058/1‑148 (MQ=255)
                                                                       aCATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGc                                                                         >  1:2916190/1‑148 (MQ=255)
                                                                             ttACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCaa                                                                   <  2:3357701/148‑1 (MQ=255)
                                                                              tACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTTAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGTCATCAAt                                                                  <  1:1878271/148‑1 (MQ=255)
                                                                               aCGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATa                                                                 <  2:664523/148‑1 (MQ=255)
                                                                                  tGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACaa                                                              >  1:2514254/1‑148 (MQ=255)
                                                                                    cTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAg                                                            <  1:1837076/148‑1 (MQ=255)
                                                                                     tCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGGTGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGa                                                           >  1:2096379/1‑148 (MQ=255)
                                                                                      cAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGaa                                                          <  2:3303628/148‑1 (MQ=255)
                                                                                           ctcGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATAtt                                                     <  1:2600737/148‑1 (MQ=255)
                                                                                           ctcGCGTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATAtt                                                     <  2:2128286/148‑1 (MQ=255)
                                                                                            tcGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTc                                                    <  1:889192/148‑1 (MQ=255)
                                                                                            tcGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTc                                                    <  1:3206085/148‑1 (MQ=255)
                                                                                                   ccTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGt                                             >  1:1051931/1‑148 (MQ=255)
                                                                                                   ccTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGt                                             >  1:2827272/1‑148 (MQ=255)
                                                                                                     tGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTa                                           <  2:1637758/148‑1 (MQ=255)
                                                                                                      gggTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTaa                                          >  2:2248139/1‑147 (MQ=255)
                                                                                                       ggTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTAcc                                         >  2:1877733/1‑148 (MQ=255)
                                                                                                          tATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTTAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCaa                                      >  1:144317/1‑148 (MQ=255)
                                                                                                            tCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAAcc                                    >  2:2627241/1‑148 (MQ=255)
                                                                                                            tCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAAcc                                    <  2:2787445/148‑1 (MQ=255)
                                                                                                            tCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAAcc                                    >  2:3241567/1‑148 (MQ=255)
                                                                                                              gggCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCg                                  <  1:3439952/148‑1 (MQ=255)
                                                                                                               ggCCACCGCTCGCTTCTGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGt                                 <  1:2482507/148‑1 (MQ=255)
                                                                                                               ggCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGt                                 <  1:977459/148‑1 (MQ=255)
                                                                                                               ggCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGt                                 >  2:344716/1‑148 (MQ=255)
                                                                                                               ggCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGt                                 <  1:303240/148‑1 (MQ=255)
                                                                                                                 ccACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAg                               <  2:407385/148‑1 (MQ=255)
                                                                                                                    ccGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGaaa                            >  2:826550/1‑148 (MQ=255)
                                                                                                                    ccGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGaaa                            <  1:3027343/148‑1 (MQ=255)
                                                                                                                    ccGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGaaa                            <  1:3404833/148‑1 (MQ=255)
                                                                                                                    ccGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTAACAGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGaaa                            >  2:826630/1‑148 (MQ=255)
                                                                                                                     cgctcgctTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAAt                           >  1:3546076/1‑148 (MQ=255)
                                                                                                                         cgctTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTa                       >  1:1632858/1‑148 (MQ=255)
                                                                                                                         cgctTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTa                       <  1:1361081/148‑1 (MQ=255)
                                                                                                                          gctTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTAc                      <  2:3430268/148‑1 (MQ=255)
                                                                                                                          gctTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTAc                      >  2:1660674/1‑148 (MQ=255)
                                                                                                                           ctTATGAACTGGCAATGGGATTTAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTAcc                     >  1:2129948/1‑148 (MQ=255)
                                                                                                                              aTGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTc                  >  2:3485906/1‑148 (MQ=255)
                                                                                                                              aTGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTc                  >  2:1728177/1‑148 (MQ=255)
                                                                                                                                gAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTcca                <  2:1124182/148‑1 (MQ=255)
                                                                                                                                gAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTcca                >  2:2472811/1‑148 (MQ=255)
                                                                                                                                   cTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTccaccc             <  2:2970299/148‑1 (MQ=255)
                                                                                                                                     ggaaaTGGGATTGAGTTTTAAAGTGATGTATCTCACCTCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTccacccac           <  2:2415148/145‑1 (MQ=255)
                                                                                                                                         aTGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTCCACCCACAGGa       >  2:56549/1‑148 (MQ=255)
                                                                                                                                          tGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTCCACCCACAGGaa      <  2:785623/148‑1 (MQ=255)
                                                                                                                                           gggATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTCCACCCACAGGAAg     <  2:1736869/148‑1 (MQ=255)
                                                                                                                                             gATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTCCACCCACAGGAAGCg   >  2:52649/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTTAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTCCACCCACAGGAAGCGG  >  CP009273/3830674‑3830963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑