Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,837,532 C→A 34.4% P70Q (CCG→CAG)  ade → cryptic adenine deaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,837,5320CA34.4% 433.9 / 229.4 267P70Q (CCG→CAG) adecryptic adenine deaminase
Reads supporting (aligned to +/- strand):  ref base C (83/91);  new base A (49/43);  total (132/135)
Fisher's exact test for biased strand distribution p-value = 4.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 271 total aligned reads displayed.

TAGCCGTTTCCCGTGGCGACGCTGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGTTATTTGCGACC  >  CP009273/3837385‑3837678
                                                                                                                                                   |                                                                                                                                                  
tAGCCGTGTCCCGTGGCGACGCTGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTcc                                                                                                                                                    <  1:2533916/148‑1 (MQ=255)
      tttCCCGTGGCGACGCTGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCtgt                                                                                                                                              >  2:651420/1‑146 (MQ=255)
        tCCCGTGGCGACGCTGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGc                                                                                                                                            <  2:2702398/148‑1 (MQ=255)
                  aCGCTGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGa                                                                                                                                  >  2:2970346/1‑148 (MQ=255)
                    gCTGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATg                                                                                                                                <  1:2260799/148‑1 (MQ=255)
                        ttGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTcg                                                                                                                            >  2:1691572/1‑148 (MQ=255)
                        ttGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTcg                                                                                                                            >  2:868305/1‑148 (MQ=255)
                           ccGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCACAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCgcgg                                                                                                                         >  2:496326/1‑148 (MQ=255)
                             gATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGTAGAAATTACCGGCCCAATTGTGATTAAAGTACTTAACATTGACGGAGTTGGCGCAGAATACACTGATGCTCCGGCTTTGAAGCGGATTGATGCTCtcggcg                                                                                                                       >  1:3043613/1‑148 (MQ=255)
                             gATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCgcggcg                                                                                                                       >  2:3005281/1‑148 (MQ=255)
                                tatattatTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCaa                                                                                                                    <  1:3543331/148‑1 (MQ=255)
                                   attattGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGTTGCTCGCGGCGCAAcgg                                                                                                                 >  2:2017477/1‑148 (MQ=255)
                                   attattGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAAcgg                                                                                                                 >  2:219112/1‑148 (MQ=255)
                                   attattGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAAcgg                                                                                                                 <  2:2747794/148‑1 (MQ=255)
                                   attattGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAAcgg                                                                                                                 >  2:2026352/1‑148 (MQ=255)
                                   attattGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAAcgg                                                                                                                 <  1:131506/148‑1 (MQ=255)
                                   attattGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAAcgg                                                                                                                 >  2:2519692/1‑148 (MQ=255)
                                        tGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTg                                                                                                            >  1:3365304/1‑148 (MQ=255)
                                         gATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGc                                                                                                           <  1:504140/148‑1 (MQ=255)
                                          aTAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGcc                                                                                                          <  2:714276/148‑1 (MQ=255)
                                           tAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCa                                                                                                         <  1:2451359/148‑1 (MQ=255)
                                            aaTGTCTCTATTCTCGACCTGATCAATGGCGTAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAg                                                                                                        >  1:2773465/1‑148 (MQ=255)
                                             aTGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAgg                                                                                                       >  1:714604/1‑148 (MQ=255)
                                               gTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGt                                                                                                     >  1:3271901/1‑148 (MQ=255)
                                                tctcTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGtt                                                                                                    <  2:2390185/148‑1 (MQ=255)
                                                tctcTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGtt                                                                                                    >  1:2638680/1‑148 (MQ=255)
                                                  tctATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTa                                                                                                  <  2:573051/148‑1 (MQ=255)
                                                  tctATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTa                                                                                                  <  1:1099926/148‑1 (MQ=255)
                                                     aTTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTg                                                                                               >  1:2755257/1‑148 (MQ=255)
                                                       tctcGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGAt                                                                                             >  2:526688/1‑148 (MQ=255)
                                                        ctcGACCTGATCAATGGCGGAGATATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATg                                                                                            <  1:2694921/148‑1 (MQ=255)
                                                          cGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCt                                                                                          <  2:2225008/148‑1 (MQ=255)
                                                             ccTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCAc                                                                                       >  1:2560823/1‑148 (MQ=255)
                                                             ccTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCAc                                                                                       >  2:42143/1‑148 (MQ=255)
                                                             ccTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCAc                                                                                       >  1:693063/1‑148 (MQ=255)
                                                              cTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCAcc                                                                                      >  2:2337973/1‑148 (MQ=255)
                                                                    aaTGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCata                                                                                <  1:59809/148‑1 (MQ=255)
                                                                    aaTGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCata                                                                                <  2:2480795/148‑1 (MQ=255)
                                                                     aTGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCatat                                                                               <  1:2951210/148‑1 (MQ=255)
                                                                       ggCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTg                                                                             <  1:3022626/148‑1 (MQ=255)
                                                                       ggCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTg                                                                             >  1:1043337/1‑148 (MQ=255)
                                                                        gCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGa                                                                            <  1:172877/148‑1 (MQ=255)
                                                                          ggAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAAt                                                                          <  2:3051398/148‑1 (MQ=255)
                                                                          ggAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAAt                                                                          <  1:2893017/148‑1 (MQ=255)
                                                                          ggAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAAt                                                                          <  1:676498/148‑1 (MQ=255)
                                                                           gagaAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATc                                                                         >  1:812238/1‑148 (MQ=255)
                                                                           gagaAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATc                                                                         >  1:86268/1‑148 (MQ=255)
                                                                            agaAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATcc                                                                        >  1:1011401/1‑148 (MQ=255)
                                                                                aTTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCa                                                                    >  2:907278/1‑148 (MQ=255)
                                                                                 tttCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCat                                                                   <  1:1691566/148‑1 (MQ=255)
                                                                                   tCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCatga                                                                 <  1:2886547/148‑1 (MQ=255)
                                                                                   tCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCatga                                                                 <  1:558130/148‑1 (MQ=255)
                                                                                     cGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCatgatg                                                               >  1:184911/1‑148 (MQ=255)
                                                                                      ggCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCAtgatga                                                              <  1:794256/148‑1 (MQ=255)
                                                                                        cccAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACg                                                            >  2:792435/1‑148 (MQ=255)
                                                                                        cccAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACg                                                            <  1:970999/148‑1 (MQ=255)
                                                                                        cccAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACg                                                            <  1:419789/148‑1 (MQ=255)
                                                                                            cttGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCgg                                                        <  2:3432037/147‑1 (MQ=255)
                                                                                            aTTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCgg                                                        >  1:2055999/1‑148 (MQ=255)
                                                                                                tgATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCAc                                                    <  1:3057111/148‑1 (MQ=255)
                                                                                                 gATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACt                                                   <  2:2956043/148‑1 (MQ=255)
                                                                                                 gATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACt                                                   >  2:3412951/1‑148 (MQ=255)
                                                                                                 gATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACt                                                   >  2:3250079/1‑148 (MQ=255)
                                                                                                   ttAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACttt                                                 >  1:3407721/1‑148 (MQ=255)
                                                                                                    tAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACtttt                                                <  1:57204/148‑1 (MQ=255)
                                                                                                    tAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACtttt                                                >  2:1953023/1‑148 (MQ=255)
                                                                                                        ggACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGaaa                                            >  2:332639/1‑148 (MQ=255)
                                                                                                         gACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAAc                                           <  2:1315257/148‑1 (MQ=255)
                                                                                                         gACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAAc                                           <  2:3085392/148‑1 (MQ=255)
                                                                                                           cGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCg                                         <  2:3115528/148‑1 (MQ=255)
                                                                                                             ttACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCt                                       <  1:970602/148‑1 (MQ=255)
                                                                                                              tACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTa                                      >  1:2362278/1‑148 (MQ=255)
                                                                                                              tACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTa                                      >  1:450249/1‑148 (MQ=255)
                                                                                                              tACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTa                                      >  1:2241178/1‑148 (MQ=255)
                                                                                                              tACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTa                                      >  2:377946/1‑148 (MQ=255)
                                                                                                              tACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTa                                      >  1:735024/1‑148 (MQ=255)
                                                                                                               aCATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTAc                                     >  2:129589/1‑148 (MQ=255)
                                                                                                               aCATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTAc                                     <  1:2819314/148‑1 (MQ=255)
                                                                                                                cATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTAcc                                    <  1:2150022/148‑1 (MQ=255)
                                                                                                                 aTTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTAccc                                   <  2:1870385/148‑1 (MQ=255)
                                                                                                                   tGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTg                                 <  1:1623896/148‑1 (MQ=255)
                                                                                                                    gCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGc                                >  2:1824216/1‑148 (MQ=255)
                                                                                                                        gtgtTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCcgcg                            >  2:1764017/1‑148 (MQ=255)
                                                                                                                           ttGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCgg                         <  2:1680307/148‑1 (MQ=255)
                                                                                                                           ttGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCgg                         <  2:2824846/148‑1 (MQ=255)
                                                                                                                           ttGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCgg                         >  1:3157486/1‑148 (MQ=255)
                                                                                                                           ttGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCgg                         >  1:3159147/1‑148 (MQ=255)
                                                                                                                             ggCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGcc                       <  2:184911/148‑1 (MQ=255)
                                                                                                                             ggCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGcc                       <  2:1074069/148‑1 (MQ=255)
                                                                                                                             ggCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGcc                       <  1:1935243/148‑1 (MQ=255)
                                                                                                                               cgcAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTg                     <  1:1672923/148‑1 (MQ=255)
                                                                                                                                gcAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTga                    <  2:2866047/148‑1 (MQ=255)
                                                                                                                                 cAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTgac                   >  1:3290429/1‑148 (MQ=255)
                                                                                                                                 cAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTgac                   <  2:1043337/148‑1 (MQ=255)
                                                                                                                                 cAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTgac                   >  2:1487472/1‑148 (MQ=255)
                                                                                                                                   gAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTgacga                 <  2:741356/148‑1 (MQ=255)
                                                                                                                                        cacTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGtt            <  1:3005281/148‑1 (MQ=255)
                                                                                                                                         acTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTAACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGTTa           <  1:1422632/148‑1 (MQ=255)
                                                                                                                                          cTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGTTAt          <  2:2115548/148‑1 (MQ=255)
                                                                                                                                              tGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGTTATTTGc      <  2:2638680/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TAGCCGTTTCCCGTGGCGACGCTGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGTTATTTGCGACC  >  CP009273/3837385‑3837678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑