BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,873,695	C→A	5.1%	R296L (CGG→CTG)	recF ←	gap repair protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,873,695	0	C	A	5.1%	754.2 / 11.1	195	R296L (CGG→CTG)	recF	gap repair protein
Reads supporting (aligned to +/- strand): ref base C (106/79); new base A (7/3); total (113/82)
Fisher's exact test for biased strand distribution p-value = 5.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 214 total aligned reads displayed.

AATTTTCGTCCGACATGTCTATAACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGG  >  CP009273/3873549‑3873842

aagtttCGTCCGACATGTCTATAACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACAccg                                                                                                                                                    <  2:3130192/145‑1 (MQ=255)

aaTTTTCGTCCGACATGTCTATAACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACAccg                                                                                                                                                    <  1:779141/148‑1 (MQ=255)

 aTTTTCGTCCGACATGTCTATAACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACAccgc                                                                                                                                                   >  1:1825829/1‑148 (MQ=255)

 aTTTTCGTCCGACATGTCTATAACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACAccgc                                                                                                                                                   >  1:1825776/1‑148 (MQ=255)

 aTTTTCGTCCGACATGTCTATAACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACAccgc                                                                                                                                                   >  2:2912218/1‑148 (MQ=255)

 aTTTTCGTCCGACATGTCTATAACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTGAATCGGCTGGCAAGCAGCCCGCGACGCTCATCATAAAGCTCAGAGGCAAAATCATCTATCAGGTAGATACAccgc                                                                                                                                                   >  1:2400929/1‑148 (MQ=255)

   tttCGTCCGACATGTCTATAACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACAccgccg                                                                                                                                                 >  1:896355/1‑148 (MQ=255)

    ttCGTCCGACATGTCTATAACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACcgccgc                                                                                                                                                <  2:2109267/148‑1 (MQ=255)

          cGACATGTCTATAACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCtt                                                                                                                                          <  2:3053916/148‑1 (MQ=255)

          cGACATGTCTATAACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCtt                                                                                                                                          <  2:2707924/148‑1 (MQ=255)

             cATGTCTATAACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAATCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCa                                                                                                                                       >  1:978483/1‑148 (MQ=255)

             cATGTCTATAACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCa                                                                                                                                       >  2:2364373/1‑148 (MQ=255)

                   tataACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTg                                                                                                                                 <  2:365612/148‑1 (MQ=255)

                    ataACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCGTTCACGGGGGa                                                                                                                                >  2:1421785/1‑148 (MQ=255)

                       aCGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGa                                                                                                                             <  2:2173978/148‑1 (MQ=255)

                        cGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGaa                                                                                                                            >  2:3414727/1‑148 (MQ=255)

                         gtgtTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAAc                                                                                                                           <  1:3495225/148‑1 (MQ=255)

                          tgtTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAAct                                                                                                                          >  1:1009826/1‑148 (MQ=255)

                             tCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACtctc                                                                                                                       >  1:977326/1‑148 (MQ=255)

                             tCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACtctc                                                                                                                       >  2:1322507/1‑148 (MQ=255)

                             tCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACtctc                                                                                                                       >  1:978172/1‑148 (MQ=255)

                             tCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACtctc                                                                                                                       >  2:3551143/1‑148 (MQ=255)

                              cAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTcc                                                                                                                      >  2:1237306/1‑148 (MQ=255)

                                 cACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTg                                                                                                                   <  1:155083/148‑1 (MQ=255)

                                 cACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTg                                                                                                                   >  2:2313814/1‑148 (MQ=255)

                                 cACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCGTTCACGGGTGAGGAACTCTCCTTg                                                                                                                   >  1:333116/1‑148 (MQ=255)

                                 cACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACAACGCCGCCCGCTTTAACGGGTGAGGAACTCTCCATg                                                                                                                   >  1:2676873/1‑148 (MQ=255)

                                     gATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGcc                                                                                                               >  1:1760955/1‑148 (MQ=255)

                                        cgcgCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGa                                                                                                            <  1:1288652/148‑1 (MQ=255)

                                        cgcgCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGa                                                                                                            <  1:1222693/148‑1 (MQ=255)

                                          cgcTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACg                                                                                                          >  2:1110743/1‑148 (MQ=255)

                                               aCAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAgg                                                                                                     >  1:1155439/1‑148 (MQ=255)

                                                cAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGgc                                                                                                    >  2:62745/1‑148 (MQ=255)

                                                  aaGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGgcgc                                                                                                  >  2:2749937/1‑148 (MQ=255)

                                                    gACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGcac                                                                                                <  1:2002374/148‑1 (MQ=255)

                                                    gACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGcac                                                                                                >  2:1651853/1‑148 (MQ=255)

                                                    gACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGcac                                                                                                <  2:3300443/148‑1 (MQ=255)

                                                          tgATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTAAGAGGCAAAATCATCTATCAGGTAGATACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTTCGCCAGACGTAAGGCGCACATCAAc                                                                                          >  1:2148951/1‑148 (MQ=255)

                                                           gATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACa                                                                                         >  1:1410874/1‑148 (MQ=255)

                                                              tGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCt                                                                                      <  1:1264836/148‑1 (MQ=255)

                                                              tGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCt                                                                                      <  1:1263041/148‑1 (MQ=255)

                                                                      ttttAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGc                                                                              >  1:692424/1‑148 (MQ=255)

                                                                       tttAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGcc                                                                             >  2:2611544/1‑148 (MQ=255)

                                                                       tttAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGcc                                                                             >  2:1075458/1‑148 (MQ=255)

                                                                       tttAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGcc                                                                             >  2:862546/1‑148 (MQ=255)

                                                                        ttAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGccc                                                                            >  1:556394/1‑148 (MQ=255)

                                                                          aaGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCAc                                                                          >  1:208572/1‑148 (MQ=255)

                                                                           aGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCAcg                                                                         <  1:2859922/148‑1 (MQ=255)

                                                                            gCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACAGCAACAGCTTAAGCTGCCCAcgc                                                                        >  2:2494525/1‑148 (MQ=255)

                                                                              ggCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGa                                                                      <  1:2906284/148‑1 (MQ=255)

                                                                                cTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATa                                                                    <  1:2253934/148‑1 (MQ=255)

                                                                                  ggCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAg                                                                  <  2:977326/148‑1 (MQ=255)

                                                                                  ggCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAg                                                                  <  2:978172/148‑1 (MQ=255)

                                                                                    cAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGt                                                                <  2:1902538/148‑1 (MQ=255)

                                                                                     aaGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTa                                                               <  1:1760099/148‑1 (MQ=255)

                                                                                      agcagcCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTAt                                                              <  1:1429877/148‑1 (MQ=255)

                                                                                       gcagcCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATc                                                             >  2:3250065/1‑148 (MQ=255)

                                                                                        cagcCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCt                                                            <  2:2547467/148‑1 (MQ=255)

                                                                                        cagcCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCt                                                            <  1:1857117/148‑1 (MQ=255)

                                                                                           cccGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTcc                                                         <  1:3532528/148‑1 (MQ=255)

                                                                                           cccGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTcc                                                         >  1:2250106/1‑148 (MQ=255)

                                                                                           cccGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTcc                                                         >  1:2842298/1‑148 (MQ=255)

                                                                                              gcgACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCAcc                                                      >  2:2568468/1‑148 (MQ=255)

                                                                                               cgACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCg                                                     >  2:235897/1‑148 (MQ=255)

                                                                                                gACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCgg                                                    <  1:1488224/148‑1 (MQ=255)

                                                                                                  cGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGgcg                                                  >  2:1805687/1‑148 (MQ=255)

                                                                                                   gCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGgcgc                                                 >  1:2589614/1‑148 (MQ=255)

                                                                                                    cTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCa                                                <  2:3062644/148‑1 (MQ=255)

                                                                                                       atcatcaAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCg                                             <  1:1335502/148‑1 (MQ=255)

                                                                                                        tcatcaAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGt                                            <  1:492100/148‑1 (MQ=255)

                                                                                                         catcaAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTCTCTTCCACCGGCGCACCGTc                                           >  2:2934394/1‑148 (MQ=255)

                                                                                                          atcaAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCg                                          >  2:2863568/1‑148 (MQ=255)

                                                                                                           tcaAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCgg                                         >  2:1437654/1‑148 (MQ=255)

                                                                                                            caAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGgc                                        >  1:30440/1‑148 (MQ=255)

                                                                                                                cTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGa                                    <  1:2357797/148‑1 (MQ=255)

                                                                                                                cTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGa                                    >  2:823271/1‑148 (MQ=255)

                                                                                                                cTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGa                                    >  1:2445865/1‑148 (MQ=255)

                                                                                                                   agagGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATg                                 >  2:3409395/1‑148 (MQ=255)

                                                                                                                     agGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCg                               >  1:1883546/1‑148 (MQ=255)

                                                                                                                       gCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTa                             >  1:2417692/1‑148 (MQ=255)

                                                                                                                           aaTCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGACTGCGTAAGTc                         >  2:702899/1‑148 (MQ=255)

                                                                                                                           aaTCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTc                         <  1:1414493/148‑1 (MQ=255)

                                                                                                                           aaTCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTc                         <  1:3237242/148‑1 (MQ=255)

                                                                                                                            atcatcTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTcc                        >  1:1127525/1‑148 (MQ=255)

                                                                                                                              catcTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGc                      >  2:57631/1‑148 (MQ=255)

                                                                                                                               atcTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCt                     <  2:1559802/148‑1 (MQ=255)

                                                                                                                                  tATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTtg                  <  1:1033058/148‑1 (MQ=255)

                                                                                                                                  tATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTtg                  >  2:417347/1‑148 (MQ=255)

                                                                                                                                   aTCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTtgt                 >  1:3261455/1‑148 (MQ=255)

                                                                                                                                    tCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTtgtg                >  2:1072629/1‑148 (MQ=255)

                                                                                                                                    tCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTtgtg                >  2:905656/1‑148 (MQ=255)

                                                                                                                                     cAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGc               >  2:2034383/1‑148 (MQ=255)

                                                                                                                                       ggTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCgg             >  1:2296929/1‑148 (MQ=255)

                                                                                                                                       ggTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCgg             >  1:1193822/1‑148 (MQ=255)

                                                                                                                                        gTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAAGTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCNTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCggg            >  1:1801767/1‑148 (MQ=255)

                                                                                                                                        gTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCggg            <  1:3006685/148‑1 (MQ=255)

                                                                                                                                        gTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCggg            <  1:749517/148‑1 (MQ=255)

                                                                                                                                           gagaCACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCg         <  1:2903821/148‑1 (MQ=255)

                                                                                                                                                 ccgccgCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGcgcg   <  1:3073277/148‑1 (MQ=255)

                                                                                                                                                  cgccgcCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCgg  <  2:2183604/148‑1 (MQ=255)

AATTTTCGTCCGACATGTCTATAACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGG  >  CP009273/3873549‑3873842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`