Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,916,830 C→A 100% D71Y (GAT→TAT)  rsmG ← 16S rRNA m(7)G527 methyltransferase, SAM‑dependent; glucose‑inhibited cell‑division protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,916,8300CA100.0% 540.1 / ‑6.7 162D71Y (GAT→TAT) rsmG16S rRNA m(7)G527 methyltransferase, SAM‑dependent; glucose‑inhibited cell‑division protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (73/88);  minor base T (1/0);  total (74/88)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 162 total aligned reads displayed.

GTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATAAG  >  CP009273/3916686‑3916971
                                                                                                                                                |                                                                                                                                             
  tCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATaa                                                                                                                                          <  2:1587283/148‑1 (MQ=255)
      tatTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCg                                                                                                                                      <  2:340599/148‑1 (MQ=255)
      tatTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCg                                                                                                                                      >  2:1906120/1‑148 (MQ=255)
      tatTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCg                                                                                                                                      <  1:523880/148‑1 (MQ=255)
       atTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGt                                                                                                                                     <  2:239573/148‑1 (MQ=255)
       atTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGt                                                                                                                                     <  2:239148/148‑1 (MQ=255)
       atTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGt                                                                                                                                     >  1:1840531/1‑148 (MQ=255)
        ttCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGtt                                                                                                                                    >  1:1554244/1‑148 (MQ=255)
         tctcCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTc                                                                                                                                   >  1:1321434/1‑148 (MQ=255)
           tcCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCAc                                                                                                                                 >  1:43528/1‑148 (MQ=255)
              aGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCtt                                                                                                                              >  2:484863/1‑148 (MQ=255)
              aGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCtt                                                                                                                              >  2:484856/1‑148 (MQ=255)
                tttAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGc                                                                                                                            <  1:1789638/148‑1 (MQ=255)
                 ttAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCa                                                                                                                           >  1:880058/1‑148 (MQ=255)
                 ttAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCa                                                                                                                           >  1:714077/1‑148 (MQ=255)
                  tAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAg                                                                                                                          <  1:2643500/148‑1 (MQ=255)
                  tAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAg                                                                                                                          <  1:1192480/148‑1 (MQ=255)
                    aGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGAt                                                                                                                        <  1:2706636/148‑1 (MQ=255)
                    aGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGAt                                                                                                                        >  1:330939/1‑148 (MQ=255)
                    aGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGAt                                                                                                                        <  1:3504819/148‑1 (MQ=255)
                     gCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATa                                                                                                                       <  2:1090819/148‑1 (MQ=255)
                        cATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACgg                                                                                                                    >  1:1222292/1‑148 (MQ=255)
                           gTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGc                                                                                                                 <  2:1318597/148‑1 (MQ=255)
                           gTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGc                                                                                                                 >  1:2935099/1‑148 (MQ=255)
                           gTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGc                                                                                                                 <  1:421739/148‑1 (MQ=255)
                           gTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGc                                                                                                                 >  2:2608682/1‑148 (MQ=255)
                             tGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGcca                                                                                                               >  2:1021499/1‑148 (MQ=255)
                                 ccTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCcaccac                                                                                                           >  2:290265/1‑148 (MQ=255)
                                 ccTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCcaccac                                                                                                           >  1:1041088/1‑148 (MQ=255)
                                     aCGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATg                                                                                                       <  1:495160/148‑1 (MQ=255)
                                     aCGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATg                                                                                                       <  1:3387074/148‑1 (MQ=255)
                                     aCGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATg                                                                                                       >  2:1398897/1‑148 (MQ=255)
                                      cGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCTCCTTGCAGATACGGTGCCACCACAATGc                                                                                                      <  2:1544546/148‑1 (MQ=255)
                                       gAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCt                                                                                                     <  1:1499381/148‑1 (MQ=255)
                                        aaGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTa                                                                                                    >  2:303167/1‑148 (MQ=255)
                                         aGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTAt                                                                                                   <  2:1643968/148‑1 (MQ=255)
                                         aGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTAt                                                                                                   >  2:2503110/1‑148 (MQ=255)
                                         aGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTAt                                                                                                   >  2:2503352/1‑148 (MQ=255)
                                         aGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTAt                                                                                                   >  1:1679593/1‑148 (MQ=255)
                                         aGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGGTCACCTTGCAGATACGGTGCCACCACAATGCTAt                                                                                                   >  2:3308449/1‑148 (MQ=255)
                                            aaaCGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCga                                                                                                <  1:2942280/148‑1 (MQ=255)
                                             aaCGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCgag                                                                                               <  1:3439096/148‑1 (MQ=255)
                                             aaCGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCgag                                                                                               <  1:3439118/148‑1 (MQ=255)
                                              acgcacgcGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCgaga                                                                                              <  2:3115340/148‑1 (MQ=255)
                                                gcacgcGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAat                                                                                            <  1:2601627/148‑1 (MQ=255)
                                                   cgcgTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATg                                                                                         >  2:1523337/1‑148 (MQ=255)
                                                      gTTTACCAAGGCTATCCATCAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACACTGCTATCGAGAATATGGCg                                                                                      <  2:3220792/148‑1 (MQ=255)
                                                      gTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGTCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCg                                                                                      <  1:3064917/148‑1 (MQ=255)
                                                          aCCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTAcc                                                                                  <  2:368153/148‑1 (MQ=255)
                                                            cAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAg                                                                                <  2:2854862/148‑1 (MQ=255)
                                                               ggCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCAt                                                                             >  2:1608324/1‑148 (MQ=255)
                                                               ggCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCAt                                                                             >  2:1608280/1‑148 (MQ=255)
                                                               ggCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCAt                                                                             <  2:2234762/148‑1 (MQ=255)
                                                                gCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCAtc                                                                            <  2:1814015/148‑1 (MQ=255)
                                                                 cTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCAtct                                                                           >  2:776515/1‑148 (MQ=255)
                                                                  tATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCAtctc                                                                          <  1:1695505/148‑1 (MQ=255)
                                                                   aTCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCa                                                                         <  2:1632141/148‑1 (MQ=255)
                                                                   aTCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCa                                                                         >  2:1524656/1‑148 (MQ=255)
                                                                    tCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCAt                                                                        <  2:2638949/148‑1 (MQ=255)
                                                                      cAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTa                                                                      <  2:421822/148‑1 (MQ=255)
                                                                          agagTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGAt                                                                  >  2:1092268/1‑148 (MQ=255)
                                                                          agagTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGAt                                                                  <  2:1407338/148‑1 (MQ=255)
                                                                              tGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATcgcg                                                              >  2:1832136/1‑148 (MQ=255)
                                                                              tGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATcgcg                                                              >  2:1846777/1‑148 (MQ=255)
                                                                              tGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATcgcg                                                              <  2:880058/148‑1 (MQ=255)
                                                                               gAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCgg                                                             <  1:1745608/148‑1 (MQ=255)
                                                                                aaaTGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGa                                                            <  2:1434506/148‑1 (MQ=255)
                                                                                  aTGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGAcc                                                          >  2:1080629/1‑148 (MQ=255)
                                                                                    gggCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGa                                                        >  1:2292782/1‑148 (MQ=255)
                                                                                      gCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAg                                                      <  2:1088658/148‑1 (MQ=255)
                                                                                      gCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAg                                                      <  1:1906120/148‑1 (MQ=255)
                                                                                       cTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGt                                                     >  1:488670/1‑148 (MQ=255)
                                                                                         tCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCa                                                   <  2:1487781/148‑1 (MQ=255)
                                                                                             gACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGtt                                               >  1:3105559/1‑148 (MQ=255)
                                                                                              aCGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTg                                              <  1:2720369/148‑1 (MQ=255)
                                                                                                  aCGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACg                                          <  1:2952801/148‑1 (MQ=255)
                                                                                                   cGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGc                                         >  2:2469468/1‑148 (MQ=255)
                                                                                                       agagagTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTg                                     >  1:2510867/1‑148 (MQ=255)
                                                                                                         agagTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGtt                                   >  2:2735599/1‑148 (MQ=255)
                                                                                                              ggAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCAtt                              >  1:2047368/1‑148 (MQ=255)
                                                                                                                aaTGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTa                            <  1:585457/148‑1 (MQ=255)
                                                                                                                 aTGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTAt                           >  2:3188392/1‑148 (MQ=255)
                                                                                                                  tGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCCTCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCAGTTATg                          >  1:3121955/1‑148 (MQ=255)
                                                                                                                     cTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATgcag                       >  2:402380/1‑148 (MQ=255)
                                                                                                                      tGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATgcagc                      <  1:2798839/148‑1 (MQ=255)
                                                                                                                       ggCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATgcagca                     <  2:3471060/148‑1 (MQ=255)
                                                                                                                        gCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCat                    >  1:2193290/1‑148 (MQ=255)
                                                                                                                        gCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCat                    <  2:855035/148‑1 (MQ=255)
                                                                                                                        gCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCat                    >  1:2034845/1‑148 (MQ=255)
                                                                                                                         cAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCata                   <  1:1632159/148‑1 (MQ=255)
                                                                                                                         cAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCata                   <  1:1631892/148‑1 (MQ=255)
                                                                                                                         cAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCata                   >  1:1536071/1‑148 (MQ=255)
                                                                                                                          aGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCatat                  >  1:494762/1‑148 (MQ=255)
                                                                                                                           gTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATAtt                 >  1:515650/1‑148 (MQ=255)
                                                                                                                            tCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTc                >  1:3025804/1‑148 (MQ=255)
                                                                                                                             ccTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCa               <  2:3529035/148‑1 (MQ=255)
                                                                                                                              cTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCAc              <  1:2369405/148‑1 (MQ=255)
                                                                                                                              cTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCAc              >  1:2973254/1‑148 (MQ=255)
                                                                                                                                   ccGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCACGTAgg         <  2:633695/148‑1 (MQ=255)
                                                                                                                                          cGACATAGATAAACCGGTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATAAg  <  2:2173632/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                             
GTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATAAG  >  CP009273/3916686‑3916971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑