Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,917,554 C→A 100% L480L (CTG→CTT)  mnmG ← 5‑methylaminomethyl‑2‑thiouridine modification at tRNA U34

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,917,5540CA97.2% 574.5 / 6.5 193L480L (CTG→CTT) mnmG5‑methylaminomethyl‑2‑thiouridine modification at tRNA U34
Reads supporting (aligned to +/- strand):  ref base C (5/1);  new base A (91/96);  total (96/97)
Fisher's exact test for biased strand distribution p-value = 1.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 193 total aligned reads displayed.

AACGGCGTCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAGACGATATTCTGCG  >  CP009273/3917408‑3917698
                                                                                                                                                  |                                                                                                                                                
aaCGGCGTCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCa                                                                                                                                                 >  1:2382390/1‑148 (MQ=255)
 aCGGCGTCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAg                                                                                                                                                >  1:1071510/1‑148 (MQ=255)
 aCGGCGTCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAg                                                                                                                                                <  2:721303/148‑1 (MQ=255)
  cGGCGTCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAga                                                                                                                                               >  2:2062288/1‑148 (MQ=255)
  cGGCGTCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAga                                                                                                                                               <  2:3394878/148‑1 (MQ=255)
  cGGCGTCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAga                                                                                                                                               >  2:2062086/1‑148 (MQ=255)
  cGGCGTCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAga                                                                                                                                               >  1:2999601/1‑148 (MQ=255)
     cGTCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGAGTTAAgacgc                                                                                                                                            >  2:3533549/1‑148 (MQ=255)
       tCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAgacgctg                                                                                                                                          >  1:3198153/1‑148 (MQ=255)
        cAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAgacgctga                                                                                                                                         >  2:3310040/1‑148 (MQ=255)
        cAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAgacgctga                                                                                                                                         >  2:2787271/1‑148 (MQ=255)
          gCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAgacgctgacg                                                                                                                                       <  1:1814321/148‑1 (MQ=255)
          gCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAgacgctgacg                                                                                                                                       <  1:1814432/148‑1 (MQ=255)
              ggTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGacgctca                                                                                                                                   >  2:1853222/1‑148 (MQ=255)
                ttAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGacgctcacg                                                                                                                                 >  1:2229738/1‑148 (MQ=255)
                      tttCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGat                                                                                                                           <  1:2157961/148‑1 (MQ=255)
                        tCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGatat                                                                                                                         <  2:3237271/148‑1 (MQ=255)
                           tAAGTCAGTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATtct                                                                                                                      <  2:2121917/148‑1 (MQ=255)
                            aaGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATtctc                                                                                                                     >  1:692033/1‑148 (MQ=255)
                              gTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCaa                                                                                                                   >  2:1356381/1‑148 (MQ=255)
                              gTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCaa                                                                                                                   >  2:1356518/1‑148 (MQ=255)
                               tCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAg                                                                                                                  >  2:3413184/1‑148 (MQ=255)
                                cATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGt                                                                                                                 <  1:3271202/148‑1 (MQ=255)
                                cATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGt                                                                                                                 <  2:1525640/148‑1 (MQ=255)
                                cATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGt                                                                                                                 <  2:1648042/148‑1 (MQ=255)
                                 atgtCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGtt                                                                                                                <  2:2267633/145‑1 (MQ=255)
                                 aTTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGtt                                                                                                                >  2:2504980/1‑148 (MQ=255)
                                 aTTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGtt                                                                                                                >  2:812677/1‑148 (MQ=255)
                                  tttCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGAAGCTTACGCTCACGCTCGATATTCTCAAGttt                                                                                                               >  1:2553225/1‑148 (MQ=255)
                                   ttCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGAGTTAAGACGCTGACGCTCACGCGCGATATTCTCAAGTTtc                                                                                                              >  1:310788/1‑148 (MQ=255)
                                        gacgacGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGtt                                                                                                         >  1:1676098/1‑148 (MQ=255)
                                        gacgacGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGtt                                                                                                         >  1:3435311/1‑148 (MQ=255)
                                         acgacgCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTa                                                                                                        >  1:1591726/1‑148 (MQ=255)
                                           gacgCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTaaa                                                                                                      <  1:1413332/148‑1 (MQ=255)
                                           gacgCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTaaa                                                                                                      <  2:928694/148‑1 (MQ=255)
                                                agcagATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAgcgcg                                                                                                 >  1:1083176/1‑148 (MQ=255)
                                                 gcagATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAgcgcgc                                                                                                <  2:3414016/148‑1 (MQ=255)
                                                  cagATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGcc                                                                                               >  2:3519908/1‑148 (MQ=255)
                                                    gATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCa                                                                                             >  1:20294/1‑148 (MQ=255)
                                                     aTCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCaa                                                                                            >  2:1697368/1‑148 (MQ=255)
                                                     aTCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCaa                                                                                            <  1:1573263/148‑1 (MQ=255)
                                                       cTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACg                                                                                          >  1:3076449/1‑148 (MQ=255)
                                                           accacTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCg                                                                                      >  1:160297/1‑148 (MQ=255)
                                                               cggtcTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCTGCTTCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCAt                                                                                  <  2:2588774/144‑1 (MQ=255)
                                                               cTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCAt                                                                                  <  2:1022844/148‑1 (MQ=255)
                                                                tGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATc                                                                                 >  2:271033/1‑148 (MQ=255)
                                                                           gAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCa                                                                      <  2:2089258/148‑1 (MQ=255)
                                                                           gAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTAATCCACCAGGCCCa                                                                      >  2:525948/1‑148 (MQ=255)
                                                                            aaaGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAg                                                                     >  2:1325911/1‑148 (MQ=255)
                                                                            aaaGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAg                                                                     >  1:2719380/1‑148 (MQ=255)
                                                                            aaaGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAg                                                                     <  2:3105608/148‑1 (MQ=255)
                                                                               gcggcgCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTc                                                                  >  1:2439587/1‑148 (MQ=255)
                                                                               gcggcgCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTc                                                                  <  1:2812180/148‑1 (MQ=255)
                                                                                cggcgCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCAACCACCAGGCCCAGTTCa                                                                 >  1:667955/1‑148 (MQ=255)
                                                                                  gcgcAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACg                                                               <  1:2278682/148‑1 (MQ=255)
                                                                                  gcgcAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACg                                                               <  2:2558556/148‑1 (MQ=255)
                                                                                   cgcAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGa                                                              <  1:1818505/148‑1 (MQ=255)
                                                                                     cAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGAcc                                                            <  1:1935159/148‑1 (MQ=255)
                                                                                       gTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGa                                                          <  1:2787271/148‑1 (MQ=255)
                                                                                       gTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGa                                                          <  2:2229738/148‑1 (MQ=255)
                                                                                        tCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGAt                                                         <  1:3310040/148‑1 (MQ=255)
                                                                                          aGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGAttt                                                       <  2:3115459/148‑1 (MQ=255)
                                                                                          aGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGAttt                                                       <  1:3019437/148‑1 (MQ=255)
                                                                                           ggTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTc                                                      >  2:2196573/1‑148 (MQ=255)
                                                                                            gTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCa                                                     <  2:692033/148‑1 (MQ=255)
                                                                                              gAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGt                                                   >  2:604216/1‑148 (MQ=255)
                                                                                              gAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGt                                                   >  1:1390165/1‑148 (MQ=255)
                                                                                                 cATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCaa                                                <  1:466296/148‑1 (MQ=255)
                                                                                                 cATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTAGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCaa                                                >  2:965078/1‑148 (MQ=255)
                                                                                                    tCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACg                                             >  1:1715630/1‑148 (MQ=255)
                                                                                                    tCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCGCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCCGGCCCAGTTCACGACCGATTTCAGTCAAACg                                             >  1:1365878/1‑148 (MQ=255)
                                                                                                     cACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGc                                            <  2:2461720/148‑1 (MQ=255)
                                                                                                       cTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAg                                          <  1:2765531/148‑1 (MQ=255)
                                                                                                          cGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATc                                       <  2:561878/148‑1 (MQ=255)
                                                                                                            gCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCg                                     <  2:3232063/148‑1 (MQ=255)
                                                                                                              tGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCa                                   <  2:3198153/148‑1 (MQ=255)
                                                                                                               gCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCAt                                  <  1:1875102/148‑1 (MQ=255)
                                                                                                               gCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCAt                                  <  1:1140392/148‑1 (MQ=255)
                                                                                                                  gCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTAt                               >  2:2396119/1‑148 (MQ=255)
                                                                                                                  gCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTAt                               >  2:3222297/1‑148 (MQ=255)
                                                                                                                    ttCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCt                             <  2:2613043/148‑1 (MQ=255)
                                                                                                                     tCCGCCGACGGGGTTACCCAGGTCGATTTAATACGCTGACGCGCACGCTCGATATTCTCAAGTTTCTAGTTAAAGCGCGCCCCACGTTCGTCATCCCCCAGGCCCAGTTCCCGACCGATTTGAGGCAAACGCAGCTCCGCATTTTCtt                            >  1:2477390/1‑148 (MQ=255)
                                                                                                                     tCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCtt                            <  1:980062/148‑1 (MQ=255)
                                                                                                                       cgccgACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTcg                          <  1:812677/148‑1 (MQ=255)
                                                                                                                       cgccgACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTcg                          <  1:2504980/148‑1 (MQ=255)
                                                                                                                          cgACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGt                       <  1:1856723/148‑1 (MQ=255)
                                                                                                                            aCGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAg                     >  2:2989560/1‑148 (MQ=255)
                                                                                                                            aCGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAg                     >  2:117776/1‑148 (MQ=255)
                                                                                                                             cGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGc                    >  2:1858321/1‑148 (MQ=255)
                                                                                                                               gggTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCAt                  <  2:1460596/148‑1 (MQ=255)
                                                                                                                               gggTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCAt                  <  2:2719380/148‑1 (MQ=255)
                                                                                                                                  ttACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAg               >  2:559064/1‑148 (MQ=255)
                                                                                                                                      ccAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAGACGa           <  2:2471581/148‑1 (MQ=255)
                                                                                                                                       cAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAGACGat          >  2:2619745/1‑148 (MQ=255)
                                                                                                                                         ggTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAGACGatat        >  1:3549193/1‑148 (MQ=255)
                                                                                                                                          gTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAGACGATAtt       <  2:2958289/148‑1 (MQ=255)
                                                                                                                                           tCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAGACGATATTc      <  1:2951286/148‑1 (MQ=255)
                                                                                                                                           tCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAGACGATATTc      <  1:1915392/148‑1 (MQ=255)
                                                                                                                                           tCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAGACGATATTc      <  2:1972196/148‑1 (MQ=255)
                                                                                                                                             gATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAGACGATATTCTg    <  2:3265335/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
AACGGCGTCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAGACGATATTCTGCG  >  CP009273/3917408‑3917698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑