Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,926,831 G→T 100% D41Y (GAT→TAT)  rbsD → putative cytoplasmic sugar‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,926,8310GT100.0% 748.1 / NA 223D41Y (GAT→TAT) rbsDputative cytoplasmic sugar‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (122/101);  total (122/101)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 225 total aligned reads displayed.

AAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAGGTCGAGGCGGCCATTATCGCGGAAGAGATCAAACACCATAATCCGCAACTCCACGAAACGTTGCTCACTCAC  >  CP009273/3926686‑3926971
                                                                                                                                                 |                                                                                                                                            
 aaCGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCtata                                                                                                                                           <  1:2000732/148‑1 (MQ=255)
  aCGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTatat                                                                                                                                          >  1:1113369/1‑148 (MQ=255)
   cGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATg                                                                                                                                         >  2:2398768/1‑148 (MQ=255)
     tgtCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGc                                                                                                                                       <  2:2813459/146‑1 (MQ=255)
      ttCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCa                                                                                                                                      >  1:1056344/1‑148 (MQ=255)
      ttCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCa                                                                                                                                      >  1:1450831/1‑148 (MQ=255)
       tCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCAt                                                                                                                                     <  2:1604639/148‑1 (MQ=255)
       tCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCAt                                                                                                                                     <  2:1600259/148‑1 (MQ=255)
        cGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCAtt                                                                                                                                    >  1:1740784/1‑148 (MQ=255)
         gCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTa                                                                                                                                   >  1:2773957/1‑148 (MQ=255)
         gCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTa                                                                                                                                   <  1:3001737/148‑1 (MQ=255)
         gCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTa                                                                                                                                   >  1:571713/1‑148 (MQ=255)
         gCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTa                                                                                                                                   <  1:3110248/148‑1 (MQ=255)
            gATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAAcc                                                                                                                                <  1:2623167/148‑1 (MQ=255)
            gATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAAcc                                                                                                                                <  1:3302741/148‑1 (MQ=255)
              tGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCa                                                                                                                              <  2:553371/148‑1 (MQ=255)
               ggAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAg                                                                                                                             <  1:2758369/148‑1 (MQ=255)
                gagaAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAgg                                                                                                                            >  1:2915529/1‑148 (MQ=255)
                gagaAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAgg                                                                                                                            >  2:2126836/1‑148 (MQ=255)
                 agaAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAggg                                                                                                                           <  2:2297129/148‑1 (MQ=255)
                 agaAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAggg                                                                                                                           <  1:2098205/148‑1 (MQ=255)
                   aaaaaaaTGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGttg                                                                                                                         >  1:1859467/1‑148 (MQ=255)
                   aaaaaaaTGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGgtg                                                                                                                         >  2:1638414/1‑148 (MQ=255)
                   aaaaaaaTGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGgtg                                                                                                                         >  2:898804/1‑148 (MQ=255)
                    aaaaaaTGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGgtgt                                                                                                                        >  2:1155013/1‑148 (MQ=255)
                      aaaaTGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTAc                                                                                                                      >  2:387139/1‑148 (MQ=255)
                         aTGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACctt                                                                                                                   >  1:3408614/1‑148 (MQ=255)
                         aTGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACctt                                                                                                                   >  1:2945556/1‑148 (MQ=255)
                          tGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACcttc                                                                                                                  <  1:1394189/148‑1 (MQ=255)
                          tGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACcttc                                                                                                                  >  1:980673/1‑148 (MQ=255)
                           gAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACcttct                                                                                                                 >  2:874579/1‑148 (MQ=255)
                           gAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACcttct                                                                                                                 <  1:1610668/148‑1 (MQ=255)
                            aaaaaaGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACcttctt                                                                                                                >  2:1408859/1‑148 (MQ=255)
                            aaaaaaGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACcttctt                                                                                                                <  2:1472977/148‑1 (MQ=255)
                            aaaaaaGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACcttctt                                                                                                                <  1:886056/148‑1 (MQ=255)
                              aaaaGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCtttt                                                                                                              >  1:1471426/1‑148 (MQ=255)
                              aaaaGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCtttt                                                                                                              >  1:665116/1‑148 (MQ=255)
                                 aGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATg                                                                                                           >  2:103928/1‑148 (MQ=255)
                                 aGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATg                                                                                                           >  2:1885152/1‑148 (MQ=255)
                                   gCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCa                                                                                                         >  2:812062/1‑148 (MQ=255)
                                   gCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCa                                                                                                         >  1:3184411/1‑148 (MQ=255)
                                    cACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAg                                                                                                        <  2:2993125/148‑1 (MQ=255)
                                     aCCGTTCTTAATTCTGATATTTCATCGGTGATATCCCGTCTGGGACATACCGATAAGCTTGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATTCAgg                                                                                                       >  1:2212550/1‑148 (MQ=255)
                                       cGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTg                                                                                                     <  2:1174324/148‑1 (MQ=255)
                                        gTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGc                                                                                                    >  1:713886/1‑148 (MQ=255)
                                              aaTTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGcg                                                                                              >  2:491428/1‑148 (MQ=255)
                                              aaTTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGcg                                                                                              >  1:531826/1‑148 (MQ=255)
                                                 tCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGcgtcg                                                                                           >  2:1711202/1‑148 (MQ=255)
                                                     atatTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTcac                                                                                       >  1:3245092/1‑148 (MQ=255)
                                                         ttCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACaaat                                                                                   >  1:1163067/1‑148 (MQ=255)
                                                         ttCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACaaat                                                                                   >  2:2645390/1‑148 (MQ=255)
                                                         ttCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACaaat                                                                                   >  2:458596/1‑148 (MQ=255)
                                                           cATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACaaatga                                                                                 >  1:2892268/1‑148 (MQ=255)
                                                            aTCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACaaatgaa                                                                                <  1:1180077/148‑1 (MQ=255)
                                                                  gATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAg                                                                          >  1:580219/1‑148 (MQ=255)
                                                                  gATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAg                                                                          >  2:1300882/1‑148 (MQ=255)
                                                                   aTCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAgg                                                                         <  1:2315287/148‑1 (MQ=255)
                                                                    tctcCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAGGt                                                                        <  2:2773992/148‑1 (MQ=255)
                                                                    tctcCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAGGt                                                                        <  2:571713/148‑1 (MQ=255)
                                                                    tctcCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAGGt                                                                        <  2:2516613/148‑1 (MQ=255)
                                                                      tcCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAGGTCg                                                                      <  2:1707192/148‑1 (MQ=255)
                                                                       cccGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAGGTCGa                                                                     >  1:2357242/1‑148 (MQ=255)
                                                                       cccGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAGGTCGa                                                                     >  1:3077149/1‑148 (MQ=255)
                                                                          gTCTGGGACATACCGATACGCTGGTTGTGTGTGATGCTGGTTTACCCATCCCCAAACGTACAACGCGTATCTAGATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAGGTCGAggc                                                                  <  1:1762448/148‑1 (MQ=255)
                                                                          gTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAGGTCGAggc                                                                  <  1:1762354/148‑1 (MQ=255)
                                                                           tCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAGGTCGAggcg                                                                 >  2:2088077/1‑148 (MQ=255)
                                                                             tGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAGGTCGAggcggc                                                               >  2:3122918/1‑148 (MQ=255)
                                                                               ggACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCTGGTCGAGGCGGCCa                                                             >  2:3401545/1‑148 (MQ=255)
                                                                               ggAAATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGACATGCAGGTCGAGGCGGCCa                                                             >  2:92213/1‑148 (MQ=255)
                                                                                gtcATACCGATTCGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCTTTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACTAATGAAATGCAGGTCGAGGCGGCCAt                                                            <  2:273981/146‑1 (MQ=255)
                                                                                gACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAGGTCGAGGCGGCCAt                                                            <  1:2942406/148‑1 (MQ=255)
                                                                                 aCATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAGGTCGAGGCGGCCAtt                                                           >  2:725268/1‑148 (MQ=255)
                                                                                   aTACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAGGTCGAGGCGGCCATTAt                                                         <  2:343430/148‑1 (MQ=255)
                                                                                    tACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAGGTCGAGGCGGCCATTATc                                                        <  2:3329554/148‑1 (MQ=255)
                                                                                     aCCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAGGTCGAGGCGGCCATTATcg                                                       <  1:335671/148‑1 (MQ=255)
                                                                                       cGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAGGTCGAGGCGGCCATTATcgcg                                                     <  2:3183541/148‑1 (MQ=255)
                                                                                         aTACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAGGTCGAGGCGGCCATTATCGCGGa                                                   >  1:3294473/1‑148 (MQ=255)
                                                                                               tggtggTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAGGTCGAGGCGGCCATTATCGCGGAAGAGAt                                             <  2:1943235/148‑1 (MQ=255)
                                                                                                    gtgtgtGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAGGTCGAGGCGGCCATTATCGCGGAAGAGATCAAac                                        <  1:103928/148‑1 (MQ=255)
                                                                                                     tgtgtgATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAGGTCGAGGCGGCCATTATCGCGGAAGAGATCAAaca                                       >  1:2026376/1‑148 (MQ=255)
                                                                                                           aTGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGTATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAGGTCGAGGCGGCCATTATCGCGGAAGAGATCAAACACCATaa                                 <  1:2398768/148‑1 (MQ=255)
                                                                                                           aTGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAGGTCGAGGCGGCCAtt                                                           <  1:3010977/122‑1 (MQ=255)
                                                                                                           aTGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAGGTCGAGGCGGCCATTATCGCGGAAGAGATCAAACACCATaa                                 >  1:2331925/1‑148 (MQ=255)
                                                                                                              cTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAGGTCGAGGCGGCCATTATCGCGGAAGAGATCAAACACCATAATcc                              >  2:1630802/1‑148 (MQ=255)
                                                                                                                  tttACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAGGTCGAGGCGGCCATTATCGCGGAAGAGATCAAACACCATAATCCGCaa                          >  2:2506750/1‑148 (MQ=255)
                                                                                                                  tttACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAGGTCGAGGCGGCCATTATCGCGGAAGAGATCAAACACCATAATCCGCaa                          <  2:2403956/148‑1 (MQ=255)
                                                                                                                  tttACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAGGTCGAGGCGGCCATTATCGCGGAAGAGATCAAACACCATAATCCGCaa                          <  2:400295/148‑1 (MQ=255)
                                                                                                                   ttACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAGGTCGAGGCGGCCATTATCGCGGAAGAGATCAAACACCATAATCCGCAAc                         >  1:3526060/1‑148 (MQ=255)
                                                                                                                      cccATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAGGTCGAGGCGGCCATTATCGCGGAAGAGATCAAACACCATAATCCGCAACTcc                      >  2:1977476/1‑148 (MQ=255)
                                                                                                                       ccATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAGGTCGAGGCGGCCATTATCGCGGAAGAGATCAAACACCATAATCCGCAACTCCa                     <  1:178634/148‑1 (MQ=255)
                                                                                                                       ccATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAGGTCGAGGCGGCCATTATCGCGGAAGAGATCAAACACCATAATCCGCAACTCCa                     <  2:302993/148‑1 (MQ=255)
                                                                                                                         aTCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAGGTCGAGGCGGCCATTATCGCGGAAGAGATCAAACACCATAATCCGCAACTCCACg                   >  2:610770/1‑148 (MQ=255)
                                                                                                                           ccccAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAGGTCGAGGCGGCCATTATCGCGGAAGAGATCAAACACCATAATCCGCAACTCCACGaa                 <  2:2165976/148‑1 (MQ=255)
                                                                                                                               aaaaGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAGGTCGAGGCGGCCATTATCGCGGAAGAGATCAAACACCATAATCCGCAACTCCACGAAACGt             >  2:378792/1‑148 (MQ=255)
                                                                                                                                 aaGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAGGTCGAGGCGGCCATTATCGCGGAAGAGATCAAACACCATAATCCGCAACTCCACGAAACGTTg           >  2:2209339/1‑148 (MQ=255)
                                                                                                                                   gTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAGGTCGAGGCGGCCATTATCGCGGAAGAGATCAAACACCATAATCCGCAACTCCACGAAACGTTGct         >  2:1574015/1‑148 (MQ=255)
                                                                                                                                    tACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAGGTCGAGGCGGCCATTATCGCGGAAGAGATCAAACACCATAATCCGCAACTCCACGAAACGTTGctc        >  1:1014238/1‑148 (MQ=255)
                                                                                                                                      cAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAGGTCGAGGCGGCCATTATCGCGGAAGAGATCAAACACCATAATCCGCAACTCCACGAAACGTTGctcac      >  1:2803931/1‑148 (MQ=255)
                                                                                                                                        aCGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAGGTCGAGGCGGCCATTATCGCTGAAGAGATCAAACACCATAATCCGCAACTCCACGAAACGTTGctcactc    >  1:127462/1‑148 (MQ=255)
                                                                                                                                          gcgTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAGGTCGAGGCGGCCATTATCGCGGAAGAGATCAAACACCATAATCCGCAACTCCACGAAACGTTGCtcactcac  >  1:3495274/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                            
AAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAGGTCGAGGCGGCCATTATCGCGGAAGAGATCAAACACCATAATCCGCAACTCCACGAAACGTTGCTCACTCAC  >  CP009273/3926686‑3926971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑