Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,931,021 C→A 93.9% A123E (GCG→GAG)  rbsK → ribokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,931,0210CA93.9% 465.6 / 21.7 164A123E (GCG→GAG) rbsKribokinase
Reads supporting (aligned to +/- strand):  ref base C (7/3);  new base A (89/65);  total (96/68)
Fisher's exact test for biased strand distribution p-value = 5.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

Only 100 of 165 total aligned reads displayed.

CAGCAGCTCGCCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGCGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCC  >  CP009273/3930876‑3931159
                                                                                                                                                 |                                                                                                                                          
  gcagcTCGCCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCtg                                                                                                                                        <  1:2646589/148‑1 (MQ=255)
   cagcTCGCCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCtgg                                                                                                                                       >  1:837016/1‑148 (MQ=255)
      cTCGCCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCtggtgg                                                                                                                                    >  2:1293159/1‑148 (MQ=255)
        cGCCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGaa                                                                                                                                  >  1:3129154/1‑148 (MQ=255)
            aCTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAgcgc                                                                                                                              >  2:1246117/1‑148 (MQ=255)
             cTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCa                                                                                                                             <  2:1474163/148‑1 (MQ=255)
               gATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAAc                                                                                                                           >  2:1943571/1‑148 (MQ=255)
                aTAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACg                                                                                                                          >  2:3470795/1‑148 (MQ=255)
                  aaCATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTg                                                                                                                        >  1:1005770/1‑148 (MQ=255)
                  aaCATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTg                                                                                                                        >  2:3223767/1‑148 (MQ=255)
                   aCATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGTTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGa                                                                                                                       <  2:2205087/148‑1 (MQ=255)
                   aCATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCACCGTGa                                                                                                                       >  2:847020/1‑148 (MQ=255)
                   aCATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGa                                                                                                                       <  2:1800572/148‑1 (MQ=255)
                   aCATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGa                                                                                                                       <  2:2457786/148‑1 (MQ=255)
                   aCATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGAATTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGa                                                                                                                       >  1:1122367/1‑148 (MQ=255)
                    cATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAg                                                                                                                      >  2:2734161/1‑148 (MQ=255)
                       tGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGt                                                                                                                   <  2:2580062/148‑1 (MQ=255)
                          tatTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTAtt                                                                                                                >  1:1759792/1‑148 (MQ=255)
                          tatTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTAtt                                                                                                                >  1:1759836/1‑148 (MQ=255)
                           atTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTg                                                                                                               >  2:3099394/1‑148 (MQ=255)
                             tACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGcc                                                                                                             >  1:340240/1‑148 (MQ=255)
                              aCTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCa                                                                                                            <  2:2976535/148‑1 (MQ=255)
                              aCTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCa                                                                                                            <  1:3224495/148‑1 (MQ=255)
                                tCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAAc                                                                                                          >  1:2942393/1‑148 (MQ=255)
                                tCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAAc                                                                                                          >  1:1170304/1‑148 (MQ=255)
                                      cAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCa                                                                                                    >  2:247204/1‑148 (MQ=255)
                                      cAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAACTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCa                                                                                                    >  2:2726690/1‑148 (MQ=255)
                                        gCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGc                                                                                                  <  2:305680/148‑1 (MQ=255)
                                         cGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCa                                                                                                 <  1:3326948/148‑1 (MQ=255)
                                         cGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCa                                                                                                 <  2:639428/148‑1 (MQ=255)
                                         cGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCa                                                                                                 >  2:1033644/1‑148 (MQ=255)
                                           tGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCatt                                                                                               <  1:1063229/148‑1 (MQ=255)
                                            gATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCatta                                                                                              >  1:1278659/1‑148 (MQ=255)
                                               cAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCAttatta                                                                                           <  2:2963537/148‑1 (MQ=255)
                                               cAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCAttatta                                                                                           >  1:710149/1‑148 (MQ=255)
                                                  aGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATg                                                                                        >  2:923115/1‑148 (MQ=255)
                                                    gCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCa                                                                                      >  1:3324460/1‑148 (MQ=255)
                                                      gAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGc                                                                                    <  2:254555/148‑1 (MQ=255)
                                                      gAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGc                                                                                    <  2:2374417/148‑1 (MQ=255)
                                                        aTCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTg                                                                                  >  2:2076879/1‑148 (MQ=255)
                                                        aTCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTg                                                                                  >  2:2076895/1‑148 (MQ=255)
                                                        aTCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTg                                                                                  >  2:2152860/1‑148 (MQ=255)
                                                        aTCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGGCAGCATTATTAATGCAGCTg                                                                                  >  2:1602659/1‑148 (MQ=255)
                                                         tCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTgg                                                                                 >  1:2071552/1‑148 (MQ=255)
                                                         tCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTgg                                                                                 >  2:1541607/1‑148 (MQ=255)
                                                         tCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTgg                                                                                 >  1:278963/1‑148 (MQ=255)
                                                          cAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGa                                                                                >  2:2372929/1‑148 (MQ=255)
                                                          cAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGa                                                                                >  2:1540514/1‑148 (MQ=255)
                                                           aaCAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGaa                                                                               <  1:2162581/148‑1 (MQ=255)
                                                            aCAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAAt                                                                              >  1:2925090/1‑148 (MQ=255)
                                                             cAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGCGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATc                                                                             <  2:2529792/148‑1 (MQ=255)
                                                                 tgtgGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATcacca                                                                         <  1:3131751/148‑1 (MQ=255)
                                                                 tgtgGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATcacca                                                                         >  2:3211744/1‑148 (MQ=255)
                                                                   tgGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACt                                                                       >  2:2620770/1‑148 (MQ=255)
                                                                     gcgcTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGCGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCg                                                                     >  2:3051677/1‑148 (MQ=255)
                                                                     gcgcTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGCGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCg                                                                     >  2:3051637/1‑148 (MQ=255)
                                                                            tttttGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAgtgt                                                              >  2:2535211/1‑148 (MQ=255)
                                                                             ttttGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGtgtg                                                             >  1:1676819/1‑148 (MQ=255)
                                                                               ttGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGAt                                                           <  2:1122367/148‑1 (MQ=255)
                                                                                 gTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATgg                                                         <  2:2045287/148‑1 (MQ=255)
                                                                                   tAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCa                                                       >  2:191890/1‑148 (MQ=255)
                                                                                         cGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAgcggcg                                                 >  2:3085007/1‑148 (MQ=255)
                                                                                         cGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAgcggcg                                                 <  1:2986881/148‑1 (MQ=255)
                                                                                         cGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAgcggcg                                                 >  2:1315369/1‑148 (MQ=255)
                                                                                          gAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGTTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGa                                                >  1:374465/1‑148 (MQ=255)
                                                                                          gAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGa                                                >  1:374322/1‑148 (MQ=255)
                                                                                           aaGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGaa                                               >  1:1452916/1‑148 (MQ=255)
                                                                                             ggTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGaaaa                                             <  2:2425009/148‑1 (MQ=255)
                                                                                             ggTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGaaaa                                             >  1:994640/1‑148 (MQ=255)
                                                                                                gagaATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCg                                          >  2:375191/1‑148 (MQ=255)
                                                                                                  gaATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGcc                                        <  2:244565/148‑1 (MQ=255)
                                                                                                   aaTGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGCGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGccc                                       >  2:529104/1‑148 (MQ=255)
                                                                                                   aaTGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGccc                                       <  2:1348920/148‑1 (MQ=255)
                                                                                                   aaTGTCATCGGTATTCATGCCGGCGATATTGCTGCCCTTTCCCCGGAGCGGGTGGAAGCGAAACGTGAGCGTTTTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCTAAAATCGccc                                       <  1:994705/148‑1 (MQ=255)
                                                                                                    aTGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGCGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCa                                      >  2:3332000/1‑148 (MQ=255)
                                                                                                     tGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCAt                                     <  2:841959/148‑1 (MQ=255)
                                                                                                      gTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATc                                    >  1:10145/1‑148 (MQ=255)
                                                                                                         aTCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCaaa                                 <  1:706722/148‑1 (MQ=255)
                                                                                                          tCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCaaaa                                >  1:2535915/1‑148 (MQ=255)
                                                                                                          tCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCaaaa                                >  2:3464519/1‑148 (MQ=255)
                                                                                                           cGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAAt                               >  1:1345186/1‑148 (MQ=255)
                                                                                                             gTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATaa                             <  1:478426/148‑1 (MQ=255)
                                                                                                               aTTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGa                           >  1:1409682/1‑148 (MQ=255)
                                                                                                                ttCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGAc                          >  1:2275426/1‑148 (MQ=255)
                                                                                                                 tCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACt                         >  2:770842/1‑148 (MQ=255)
                                                                                                                  cATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTa                        <  1:2767747/148‑1 (MQ=255)
                                                                                                                     gCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGTATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCg                     <  2:787107/148‑1 (MQ=255)
                                                                                                                        ggCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTg                  >  2:2995301/1‑148 (MQ=255)
                                                                                                                          cgcTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGACAGTGTGATGGCAGCGGCGAAACTCGCCCCTCAACATAAGACTATCGGTgcg                >  2:3418751/1‑148 (MQ=255)
                                                                                                                          cgcTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTgcg                >  1:1833214/1‑148 (MQ=255)
                                                                                                                              aaTGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTa            <  2:1400926/148‑1 (MQ=255)
                                                                                                                               aTGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTaa           <  2:1633355/148‑1 (MQ=255)
                                                                                                                               aTGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTaa           >  2:2915338/1‑148 (MQ=255)
                                                                                                                               aTGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATAAAAATAAGACTATCGTTGCGCTTaa           >  2:1830248/1‑148 (MQ=255)
                                                                                                                                   tgcCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACccg       <  1:1246117/148‑1 (MQ=255)
                                                                                                                                    gcCCTGTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACccgg      <  2:15765/148‑1 (MQ=255)
                                                                                                                                      ccTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACccggct    <  2:140961/148‑1 (MQ=255)
                                                                                                                                      ccTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACccggct    >  2:26194/1‑148 (MQ=255)
                                                                                                                                       ctgtCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACccggctc   <  1:2071999/145‑1 (MQ=255)
                                                                                                                                       cTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACccggctc   >  2:697293/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                          
CAGCAGCTCGCCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGCGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCC  >  CP009273/3930876‑3931159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑