Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,959,351 G→T 94.8% P77P (CCG→CCT)  trxA → thioredoxin 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,959,3510GT94.8% 627.4 / 22.1 213P77P (CCG→CCT) trxAthioredoxin 1
Reads supporting (aligned to +/- strand):  ref base G (8/3);  new base T (123/79);  total (131/82)
Fisher's exact test for biased strand distribution p-value = 5.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 213 total aligned reads displayed.

GGCAGAGTGGTGCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCGACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTAAAAACTGGACGCCC  >  CP009273/3959207‑3959495
                                                                                                                                                |                                                                                                                                                
  cAGAGTGGTGCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTActct                                                                                                                                             >  2:920537/1‑148 (MQ=255)
  cAGAGTGGTGCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTActct                                                                                                                                             >  1:1917894/1‑148 (MQ=255)
   agagTGGTGCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTctg                                                                                                                                            <  1:615003/148‑1 (MQ=255)
   agagTGGTGCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTctg                                                                                                                                            <  1:481359/148‑1 (MQ=255)
   agagTGGTGCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTctg                                                                                                                                            <  2:2140308/148‑1 (MQ=255)
    gagTGGTGCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTctgc                                                                                                                                           >  1:627923/1‑148 (MQ=255)
    gagTGGTGCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTctgc                                                                                                                                           <  2:2576901/148‑1 (MQ=255)
    gagTGGTGCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTctgc                                                                                                                                           >  1:2248263/1‑148 (MQ=255)
      gtggtgCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTctgctg                                                                                                                                         <  2:770071/148‑1 (MQ=255)
          tgCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGt                                                                                                                                     <  1:2552480/148‑1 (MQ=255)
          tgCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGt                                                                                                                                     <  2:751954/148‑1 (MQ=255)
               tCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCaaa                                                                                                                                >  1:1541557/1‑148 (MQ=255)
               tCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCaaa                                                                                                                                >  1:419873/1‑148 (MQ=255)
                 cGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCaaaaa                                                                                                                              >  1:2169893/1‑148 (MQ=255)
                  gTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAAc                                                                                                                             <  1:112780/148‑1 (MQ=255)
                  gTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAAc                                                                                                                             <  2:607329/148‑1 (MQ=255)
                   tGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACg                                                                                                                            >  2:3095669/1‑148 (MQ=255)
                     cAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGt                                                                                                                          <  1:648898/148‑1 (MQ=255)
                     cAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGt                                                                                                                          <  2:1607078/148‑1 (MQ=255)
                     cAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGt                                                                                                                          >  2:1569216/1‑148 (MQ=255)
                     cAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGt                                                                                                                          <  2:3400179/148‑1 (MQ=255)
                        aaTGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGaa                                                                                                                       >  2:1387469/1‑148 (MQ=255)
                        aaTGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGaa                                                                                                                       >  1:1652843/1‑148 (MQ=255)
                        aaTGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGaa                                                                                                                       >  1:396440/1‑148 (MQ=255)
                        aaTGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCGACTCTGCTGCTGTTCAAAAACGGTGaa                                                                                                                       >  1:2203606/1‑148 (MQ=255)
                        aaTGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCGACTCTGCTGCTGTTCAAAAACGGTGaa                                                                                                                       >  1:414156/1‑148 (MQ=255)
                             tCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTggc                                                                                                                  >  1:1266046/1‑148 (MQ=255)
                              cGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTggcg                                                                                                                 >  2:262732/1‑148 (MQ=255)
                                ccccGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTggcggc                                                                                                               >  2:2029444/1‑148 (MQ=255)
                                    gATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAAcc                                                                                                           >  1:1179055/1‑148 (MQ=255)
                                    gATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAAcc                                                                                                           >  1:3527569/1‑148 (MQ=255)
                                     aTTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCa                                                                                                          <  1:692775/148‑1 (MQ=255)
                                     aTTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCa                                                                                                          <  1:1385430/148‑1 (MQ=255)
                                        cTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAg                                                                                                       <  1:1184159/148‑1 (MQ=255)
                                         tGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGt                                                                                                      <  2:1476403/148‑1 (MQ=255)
                                         tGGATGAAATCGCTGACGAATATCAGGGCAAACTGAACGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGt                                                                                                      >  2:1232387/1‑148 (MQ=255)
                                           gATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTgg                                                                                                    <  1:532661/148‑1 (MQ=255)
                                           gATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTgg                                                                                                    >  2:2129665/1‑148 (MQ=255)
                                               aaaTCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGc                                                                                                <  2:1006375/148‑1 (MQ=255)
                                                aaTCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCa                                                                                               <  2:2213796/148‑1 (MQ=255)
                                                aaTCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCGACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCa                                                                                               <  1:3276650/148‑1 (MQ=255)
                                                    gCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGt                                                                                           <  1:2254398/148‑1 (MQ=255)
                                                       gACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTa                                                                                        >  1:3330314/1‑148 (MQ=255)
                                                        aCGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTaa                                                                                       <  1:1303608/148‑1 (MQ=255)
                                                        aCGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTaa                                                                                       >  1:3201813/1‑148 (MQ=255)
                                                        aCGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTaa                                                                                       >  1:291021/1‑148 (MQ=255)
                                                            atatCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGt                                                                                   >  1:2011531/1‑148 (MQ=255)
                                                            atatCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGt                                                                                   >  1:1921895/1‑148 (MQ=255)
                                                             tatCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTc                                                                                  >  2:3059699/1‑148 (MQ=255)
                                                             tatCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTc                                                                                  >  2:1501325/1‑148 (MQ=255)
                                                                  gggCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTg                                                                             <  2:29997/148‑1 (MQ=255)
                                                                  gggCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTg                                                                             <  2:1829238/148‑1 (MQ=255)
                                                                    gCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCGACTCTGCTGCTGTTCAATAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGaa                                                                           <  2:1687245/148‑1 (MQ=255)
                                                                    gCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCGACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGaa                                                                           <  2:3447427/148‑1 (MQ=255)
                                                                          tGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGtt                                                                     >  1:1716674/1‑148 (MQ=255)
                                                                           gACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTc                                                                    <  2:419873/148‑1 (MQ=255)
                                                                            aCCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTcc                                                                   <  1:2869286/148‑1 (MQ=255)
                                                                              cGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTc                                                                 >  2:840839/1‑148 (MQ=255)
                                                                              cGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTc                                                                 >  2:2299545/1‑148 (MQ=255)
                                                                                  gCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACg                                                             <  2:3508219/148‑1 (MQ=255)
                                                                                  gCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACg                                                             >  1:406907/1‑148 (MQ=255)
                                                                                   cAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGc                                                            >  2:1340677/1‑148 (MQ=255)
                                                                                   cAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGc                                                            <  2:2891152/148‑1 (MQ=255)
                                                                                     aaaCTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTa                                                          <  1:1569476/148‑1 (MQ=255)
                                                                                      aaCTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTaa                                                         <  1:44482/148‑1 (MQ=255)
                                                                                      aaCTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTaa                                                         >  1:1811821/1‑148 (MQ=255)
                                                                                         tGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCt                                                      >  1:494702/1‑148 (MQ=255)
                                                                                           aaCATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTgg                                                    >  1:2269847/1‑148 (MQ=255)
                                                                                            aCATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGc                                                   >  2:3233025/1‑148 (MQ=255)
                                                                                             cATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCg                                                  >  1:1670580/1‑148 (MQ=255)
                                                                                              aTCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGt                                                 >  2:2090909/1‑148 (MQ=255)
                                                                                               tCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTa                                                <  1:1911814/148‑1 (MQ=255)
                                                                                               tCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTa                                                <  1:2750862/148‑1 (MQ=255)
                                                                                                    cAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGa                                           >  2:3522149/1‑148 (MQ=255)
                                                                                                      aaaCCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAAt                                         <  2:1269257/148‑1 (MQ=255)
                                                                                                       aaCCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAAtt                                        >  2:1121296/1‑148 (MQ=255)
                                                                                                        aCCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAAttt                                       >  1:846889/1‑148 (MQ=255)
                                                                                                          ccTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCa                                     >  1:1588818/1‑148 (MQ=255)
                                                                                                            tGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATg                                   <  1:1220926/148‑1 (MQ=255)
                                                                                                            tGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATg                                   <  1:244396/148‑1 (MQ=255)
                                                                                                            tGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATg                                   <  1:102817/148‑1 (MQ=255)
                                                                                                                  tGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCggg                             >  1:151465/1‑148 (MQ=255)
                                                                                                                    cgcCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTg                           >  2:860041/1‑148 (MQ=255)
                                                                                                                      ccGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGcc                         <  2:517586/148‑1 (MQ=255)
                                                                                                                       cGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGccc                        >  2:2060199/1‑148 (MQ=255)
                                                                                                                        gAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGcccc                       >  1:2119072/1‑148 (MQ=255)
                                                                                                                            tatGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCg                   >  1:159340/1‑148 (MQ=255)
                                                                                                                             atGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGc                  >  2:2456412/1‑148 (MQ=255)
                                                                                                                                gCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTaa               >  2:2935263/1‑148 (MQ=255)
                                                                                                                                 cATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGACTTTCATGTTCGGGTGCCCCGTCGCTaaa              >  1:1064634/1‑148 (MQ=255)
                                                                                                                                 cATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTaaa              <  2:2990009/148‑1 (MQ=255)
                                                                                                                                  aTCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCCCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTaaaa             <  2:879211/148‑1 (MQ=255)
                                                                                                                                    ccGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTAAAAAc           >  2:2332689/1‑148 (MQ=255)
                                                                                                                                    ccGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTAAAAAc           >  2:2332646/1‑148 (MQ=255)
                                                                                                                                    ccGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTAAAAAc           >  1:3486616/1‑148 (MQ=255)
                                                                                                                                     cGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTAAAAACt          <  2:442792/148‑1 (MQ=255)
                                                                                                                                         gTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTAAAAACTGGAc      >  2:3275975/1‑148 (MQ=255)
                                                                                                                                           aTCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTAAAAACTGGACGc    <  1:3095669/148‑1 (MQ=255)
                                                                                                                                           aTCCCGACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTAAAAACTGGACGc    >  2:2764716/1‑148 (MQ=255)
                                                                                                                                            tCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTAAAAACTGGACGcc   <  2:406907/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
GGCAGAGTGGTGCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCGACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTAAAAACTGGACGCCC  >  CP009273/3959207‑3959495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑