Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 319,024 C→A 100% R326S (CGC→AGC)  ykgF → ferridoxin‑like LutB family protein; putative electron transport chain YkgEFG component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273319,0240CA97.9% 602.9 / 3.5 190R326S (CGC→AGC) ykgFferridoxin‑like LutB family protein; putative electron transport chain YkgEFG component
Reads supporting (aligned to +/- strand):  ref base C (1/3);  new base A (90/96);  total (91/99)
Fisher's exact test for biased strand distribution p-value = 6.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 192 total aligned reads displayed.

AAGCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGG  >  CP009273/318884‑319168
                                                                                                                                            |                                                                                                                                                
 aGCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATAtt                                                                                                                                          >  1:858530/1‑148 (MQ=255)
   cTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGCTGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTgg                                                                                                                                        >  1:2136181/1‑148 (MQ=255)
      ggCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCgg                                                                                                                                     >  2:949631/1‑148 (MQ=255)
      ggCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCgg                                                                                                                                     >  2:287583/1‑148 (MQ=255)
       gCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGt                                                                                                                                    >  2:222910/1‑148 (MQ=255)
        cACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTc                                                                                                                                   >  1:2775328/1‑148 (MQ=255)
         aCGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCa                                                                                                                                  <  2:220891/148‑1 (MQ=255)
            ttGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATgg                                                                                                                               >  2:2506831/1‑148 (MQ=255)
               aTGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGata                                                                                                                            >  1:294536/1‑148 (MQ=255)
                  gTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATgg                                                                                                                         <  2:2606830/148‑1 (MQ=255)
                  gTCCTGAAGAGTTTCAACTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCAGGCCGCAGAATTTCGGGATGTGCTGCGCACTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATgg                                                                                                                         >  1:2656332/1‑148 (MQ=255)
                    ccTGAAGAGTTTCATCTGGTTTTTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTGGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGct                                                                                                                       <  2:3171972/148‑1 (MQ=255)
                      tGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGctct                                                                                                                     <  2:2816542/148‑1 (MQ=255)
                      tGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGctct                                                                                                                     >  2:2156974/1‑148 (MQ=255)
                          gagTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTAttt                                                                                                                 >  2:3044542/1‑148 (MQ=255)
                           agTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTa                                                                                                                >  1:2229121/1‑148 (MQ=255)
                           agTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTa                                                                                                                >  2:62226/1‑148 (MQ=255)
                            gTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTAt                                                                                                               <  2:2557012/148‑1 (MQ=255)
                             tttCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATc                                                                                                              <  2:1730850/148‑1 (MQ=255)
                             tttCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATc                                                                                                              <  2:3269766/148‑1 (MQ=255)
                                  tCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAggg                                                                                                         >  2:1383671/1‑148 (MQ=255)
                                  tCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAggg                                                                                                         <  1:2960521/148‑1 (MQ=255)
                                     ggTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCa                                                                                                      >  1:3370807/1‑148 (MQ=255)
                                     ggTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCa                                                                                                      <  2:2355592/148‑1 (MQ=255)
                                      gTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCaa                                                                                                     >  2:2281005/1‑148 (MQ=255)
                                           tGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGt                                                                                                >  2:1368070/1‑148 (MQ=255)
                                           tGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCGGAATTTCGGGATGTGCTGCGCTGTATTAGCTGCGGGGCTTGTATGAATAATTGTACGGCAAATAGCCAAATTTGCGGTCATGGATATGGCTCTATTTATCCAGGGCCCATTGGt                                                                                                >  1:2456292/1‑148 (MQ=255)
                                           tGTCGATAACGGGCGTTCAGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAAGACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGt                                                                                                <  1:2403454/148‑1 (MQ=255)
                                            gTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTg                                                                                               <  1:193343/148‑1 (MQ=255)
                                            gTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTg                                                                                               <  1:2982483/148‑1 (MQ=255)
                                             tCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGc                                                                                              >  1:851378/1‑148 (MQ=255)
                                             tCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGc                                                                                              >  2:3541943/1‑148 (MQ=255)
                                               gATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCgg                                                                                            <  2:500121/148‑1 (MQ=255)
                                                 tAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTg                                                                                          <  2:554385/148‑1 (MQ=255)
                                                 tAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTg                                                                                          <  1:2003250/148‑1 (MQ=255)
                                                 tAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTg                                                                                          <  1:2003264/148‑1 (MQ=255)
                                                  aaCGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTGTCCAGGGCCAATTGGTGCGGTGa                                                                                         <  1:2353630/148‑1 (MQ=255)
                                                  aaCGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGa                                                                                         <  2:1847623/148‑1 (MQ=255)
                                                  aaCGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGa                                                                                         <  1:591310/148‑1 (MQ=255)
                                                    cGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGAtt                                                                                       >  2:1246331/1‑148 (MQ=255)
                                                    cGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGAtt                                                                                       >  1:2447811/1‑148 (MQ=255)
                                                    cGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGAtt                                                                                       <  2:155422/148‑1 (MQ=255)
                                                      ggCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTtc                                                                                     >  2:1058577/1‑148 (MQ=255)
                                                        cGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTtctc                                                                                   <  2:1605105/148‑1 (MQ=255)
                                                          ttCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCg                                                                                 >  2:443355/1‑148 (MQ=255)
                                                           tCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGc                                                                                >  2:2547464/1‑148 (MQ=255)
                                                           tCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGc                                                                                >  2:130722/1‑148 (MQ=255)
                                                           tCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGc                                                                                >  2:2649226/1‑148 (MQ=255)
                                                           tCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGc                                                                                <  2:257093/148‑1 (MQ=255)
                                                             tGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTa                                                                              <  2:1748216/148‑1 (MQ=255)
                                                             cgAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTa                                                                              <  1:251990/147‑1 (MQ=255)
                                                              gAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTAc                                                                             <  2:1655381/148‑1 (MQ=255)
                                                               aGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACt                                                                            <  1:900626/148‑1 (MQ=255)
                                                                ggTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACtt                                                                           >  2:178222/1‑148 (MQ=255)
                                                                ggTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACtt                                                                           <  1:2088577/148‑1 (MQ=255)
                                                                      ggCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTggcggc                                                                     >  1:129818/1‑148 (MQ=255)
                                                                        ccTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGAGATGGCTCTATTTCTCCCGGGCCAGTTGGTGCGGTGAGGTCTCCGCTACTGggcggcgg                                                                   >  1:3151703/1‑146 (MQ=255)
                                                                         ctctGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCtat                                                                  <  2:392290/148‑1 (MQ=255)
                                                                          tctGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCtata                                                                 >  1:136746/1‑148 (MQ=255)
                                                                          tctGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCCCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCtata                                                                 >  1:513839/1‑148 (MQ=255)
                                                                           ctGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTACCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaa                                                                >  1:168844/1‑148 (MQ=255)
                                                                             gAATTTCGGGATGTGCTGCGCTGTATTCTCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaag                                                              >  2:3213809/1‑148 (MQ=255)
                                                                             gAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaag                                                              <  1:410651/148‑1 (MQ=255)
                                                                             gAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaag                                                              <  1:2199182/148‑1 (MQ=255)
                                                                             gAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaag                                                              >  2:2131259/1‑148 (MQ=255)
                                                                             gAAGTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaag                                                              <  2:3201322/148‑1 (MQ=255)
                                                                              aaTTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaaga                                                             <  2:611013/148‑1 (MQ=255)
                                                                               aTTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagat                                                            >  1:2632357/1‑148 (MQ=255)
                                                                               aTTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagat                                                            >  1:2632338/1‑148 (MQ=255)
                                                                               aTTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagat                                                            >  1:3012553/1‑148 (MQ=255)
                                                                                 ttagggATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGACAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagattt                                                          >  1:3465352/4‑148 (MQ=255)
                                                                                   cGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagatttta                                                        <  1:3051391/148‑1 (MQ=255)
                                                                                       aTGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagattttaaaga                                                    >  2:3349032/1‑148 (MQ=255)
                                                                                       aTGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagattttaaaga                                                    >  1:2039337/1‑148 (MQ=255)
                                                                                           gctgcGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTa                                                <  1:2310768/148‑1 (MQ=255)
                                                                                           gctgcGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTa                                                <  1:1831830/148‑1 (MQ=255)
                                                                                             tgcGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTAcc                                              <  1:3377523/148‑1 (MQ=255)
                                                                                                   gTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGc                                        <  1:1562130/148‑1 (MQ=255)
                                                                                                    tATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGcc                                       >  1:1061519/1‑148 (MQ=255)
                                                                                                      ttCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTg                                     <  2:2048264/148‑1 (MQ=255)
                                                                                                       tCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGc                                    >  2:3274059/1‑148 (MQ=255)
                                                                                                          cTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGctct                                 <  2:2775328/148‑1 (MQ=255)
                                                                                                            gCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCttt                               <  1:3207834/148‑1 (MQ=255)
                                                                                                             cGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTa                              >  2:133168/1‑148 (MQ=255)
                                                                                                                ggCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGc                           >  1:2703576/1‑148 (MQ=255)
                                                                                                                 gCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGca                          >  1:3188342/1‑148 (MQ=255)
                                                                                                                    tGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAg                       >  2:687746/1‑148 (MQ=255)
                                                                                                                        tGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTtg                   >  1:2905747/1‑148 (MQ=255)
                                                                                                                        tGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTtg                   <  2:3410840/148‑1 (MQ=255)
                                                                                                                         gAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTtgt                  >  1:3312048/1‑148 (MQ=255)
                                                                                                                         gAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTtgt                  >  1:1598542/1‑148 (MQ=255)
                                                                                                                         gAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTtgt                  <  1:1944108/148‑1 (MQ=255)
                                                                                                                          aaTACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTtgtg                 <  1:583188/148‑1 (MQ=255)
                                                                                                                            tACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGAc               >  1:465805/1‑148 (MQ=255)
                                                                                                                              cTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACaa             >  1:1474296/1‑148 (MQ=255)
                                                                                                                                  tCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACgtg         >  1:1218793/1‑148 (MQ=255)
                                                                                                                                   ccGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACgtgt        <  1:674142/148‑1 (MQ=255)
                                                                                                                                      gCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTc     >  2:1439557/1‑148 (MQ=255)
                                                                                                                                      gCATATAGCCATAGTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTc     <  1:1357721/148‑1 (MQ=255)
                                                                                                                                         tataGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCgg  >  2:2967836/1‑148 (MQ=255)
                                                                                                                                            |                                                                                                                                                
AAGCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGG  >  CP009273/318884‑319168

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑