Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,083,906 A→C 5.6% L127R (CTG→CGG)  frvX ← putative peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,083,9060AC 5.6% 594.4 / 24.3 207L127R (CTG→CGG) frvXputative peptidase
Reads supporting (aligned to +/- strand):  ref base A (96/99);  new base C (7/5);  total (103/104)
Fisher's exact test for biased strand distribution p-value = 5.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 208 total aligned reads displayed.

TTATCCAACGCCTTGCCGACCACTTTATCTTCGCCCCAGCAGGCAAAATTGGCTTCCGGGCTAATAAAATTACCAATTTCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTACCCGGTGGTTGAGCATCGACTGATTCCACCAGCCGCCAATGGTGGTAAAACGC  >  CP009273/4083759‑4084052
                                                                                                                                                   |                                                                                                                                                  
ttATCCAACGCCTTGCCGACCACTTTATCTTCGCCCCAGCAGGCAAAATTGGCTTCCGGGCTAATAAAATTACCAATTTCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACa                                                                                                                                                    >  1:1505383/1‑148 (MQ=255)
    ccAACGCCTTGCCGACCACTTTATCTTCGCCCCAGCAGGCAAAATTGGCTTCCGGGCTAATAAAATTACCAATTTCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCgg                                                                                                                                                >  2:1098568/1‑148 (MQ=255)
    ccAACGCCTTGCCGACCACTTTATCTTCGCCCCAGCAGGCAAAATTGGCTTCCGGGCTAATAAAATTACCAATTTCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCgg                                                                                                                                                <  2:606729/148‑1 (MQ=255)
      aaCGCCTTGCCGACCACTTTATCTTCGCCCCAGCAGGCAAAATTGGCTTCCGGGCTAATAAAATTACCAATTTCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGgtt                                                                                                                                              <  1:214667/148‑1 (MQ=255)
       aCGCCTTGCCGACCACTTTATCTTCGCCCCAGCAGGCAAAATTGGCTTCCGGGCTAATAAAATTACCAATTTCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGgttg                                                                                                                                             <  1:269111/148‑1 (MQ=255)
        cGCCTTGCCGACCACTTTATCTTCGCCCCAGCAGGCAAAATTGGCTTCCGGGCTAATAAAATTACCAATTTCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGgttgt                                                                                                                                            >  1:8715/1‑148 (MQ=255)
        cGCCTTGCCGACCACTTTATCTTCGCCCCAGCAGGCAAAATTGGCTTCCGGGCTAATAAAATTACCAATTTCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGgttgt                                                                                                                                            <  1:3219638/148‑1 (MQ=255)
        cGCCTTGCCGACCACTTTATCTTCGCCCCAGCAGGCAAAATTGGCTTCCGGGCTAATAAAATTACCAATTTCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGgttgt                                                                                                                                            >  2:3299567/1‑148 (MQ=255)
        cGCCTTGCCGACCACTTTATCTTCGCCCCAGCAGGCAAAATTGGCTTCCGGGCTAATAAAATTACCAATTTCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGgttgt                                                                                                                                            <  1:1824258/148‑1 (MQ=255)
        cGCCTTGCCGACCACTTTATCTTCGCCCCAGCAGGAAAAATTGGCTTCCGGGCTAATAAAATTACCAATTTCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGgttgt                                                                                                                                            <  1:1604408/148‑1 (MQ=255)
         gCCTTGCCGACCACTTTATCTTCGCCCCAGCAGGCAAAATTGGCTTCCGGGCTAATAAAATTACCAATTTCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGgttgtt                                                                                                                                           <  1:2005001/148‑1 (MQ=255)
           cTTGCCGACCACTTTATCTTCGCCCCAGCAGGCAAAATTGGCTTCCGGGCTAATAAAATTACCAATTTCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGttgc                                                                                                                                         <  2:2656387/148‑1 (MQ=255)
            ttGCCGACCACTTTATCTTCGCCCCAGCAGGCAAAATTGGCTTCCGGGCTAATAAAATTACCAATTTCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGttgct                                                                                                                                        <  2:2037825/148‑1 (MQ=255)
             tGCCGACCACTTTATCTTCGCCCCAGCAGGCAAAATTGGCTTCCGGGCTAATAAAATTACCAATTTCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGttgctt                                                                                                                                       >  2:2549171/1‑148 (MQ=255)
               ccGACCACTTTATCTTCGCCCCAGCAGGCAAAATTGGCTTCCGGGCTAATAAAATTACCAATTTCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGttgctttt                                                                                                                                     >  1:1568087/1‑148 (MQ=255)
               ccGACCACTTTATCTTCGCCCCAGCAGGCAAAATTGGCTTCCGGGCTAATAAAATTACCAATTTCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGttgctttt                                                                                                                                     <  1:1692943/148‑1 (MQ=255)
                   ccACTTTATCTTCGCCCCAGCAGGCAAAATTGGCTTCCGGGCTAATAAAATTACCAATTTCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGttgcttttgctt                                                                                                                                 <  2:1598037/148‑1 (MQ=255)
                   ccACTTTATCTTCGCCCCAGCAGGCAAAATTGGCTTCCGGGCTAATAAAATTACCAATTTCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGttgcttttgctt                                                                                                                                 >  2:2174464/1‑148 (MQ=255)
                   ccACTTTATCGTCGCCCCAGCAGGCAAAATTGGCTTCCGGGCTAATAAAATTACCAATTTCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGttgcttttgctt                                                                                                                                 <  2:2949982/148‑1 (MQ=255)
                    cACTTTATCTTCGCCCCAGCAGGCAAAATTGGCTTCCGGGCTAATAAAATTACCAATTTCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTtgcttttgcttt                                                                                                                                >  1:2306883/1‑148 (MQ=255)
                     aCTTTATCTTCGCCCCAGCAGGCAAAATTGGCTTCCGGGCTAATAAAATTACCAATTTCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTgcttttgctttt                                                                                                                               >  1:1971802/1‑148 (MQ=255)
                        ttATCTTCGCCCCAGCAGGCAAAATTGGCTTCCGGGCTAATAAAATTACCAATTTCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCg                                                                                                                            <  1:2046127/148‑1 (MQ=255)
                              tCGCCCCAGCAGGCAAAATTGGCTTCCGGGCTAATAAAATTACCAATTTCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACg                                                                                                                      >  2:26138/1‑148 (MQ=255)
                                 ccccAGCAGGCAAAATTGGCTTCCGGGCTAATAAAATTACCAATTTCCACGCCTCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCAt                                                                                                                   >  1:2524955/1‑148 (MQ=255)
                                   ccAGCAGGCAAAATTGGCTTCCGGGCTAATAAAATTACCAATTTCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGa                                                                                                                 >  2:892695/1‑148 (MQ=255)
                                   ccAGCAGGCAAAATTGGCTTCCGGGCTAATAAAATTACCAATTTCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGa                                                                                                                 <  2:2459635/148‑1 (MQ=255)
                                    cagcagGCAAAATTGGCTTCCGGGCTAATAAAATTACCAATTTCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAg                                                                                                                <  1:981545/148‑1 (MQ=255)
                                    cagcagGCAAAATTGGCTTCCGGGCTAATAAAATTACCAATGTCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCACTATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAg                                                                                                                <  2:419692/148‑1 (MQ=255)
                                      gcagGCAAAATTGGCTTCCGGGCTAATAAAATTACCAATTTCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACCGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGgc                                                                                                              >  1:2033682/1‑148 (MQ=255)
                                       cagGCAAAATTGGCTTCCGGGCTAATAAAATTACCAATTTCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGgcg                                                                                                             >  2:3108973/1‑148 (MQ=255)
                                             aaaTTGGCTTCCGGGCTAATAAAATTACCAATTTCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACgg                                                                                                       >  1:541529/1‑148 (MQ=255)
                                              aaTTGGCTTCCGGGCTAATAAAATTACCAATTTCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGa                                                                                                      >  1:2717180/1‑148 (MQ=255)
                                               aTTGGCTTCCGGGCTAATAAAATTACCAATTTCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGaa                                                                                                     >  2:1079853/1‑148 (MQ=255)
                                               aTTGGCTTCCGGGCTAATAAAATTACCAATTTCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGaa                                                                                                     >  1:107944/1‑148 (MQ=255)
                                                   gCTTCCGGGCTAATAAAATTACCAATTTCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCaa                                                                                                 <  2:2136038/148‑1 (MQ=255)
                                                       ccGGGCTAATAAAATTACCAATTTCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAGATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATcac                                                                                             <  1:1016465/148‑1 (MQ=255)
                                                              aataaAATTACCAATTTCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAggg                                                                                      >  1:2123863/1‑148 (MQ=255)
                                                              aataaAATTACCAATTTCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAggg                                                                                      <  2:1859643/148‑1 (MQ=255)
                                                                taaAATTACCAATTTCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGAt                                                                                    <  2:2266364/148‑1 (MQ=255)
                                                                  aaaTTACCAATTTCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACCGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGAttt                                                                                  <  1:3192977/148‑1 (MQ=255)
                                                                   aaTTACCAATTTCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGAtttt                                                                                 >  1:2139842/1‑148 (MQ=255)
                                                                   aaTTACCAATTTCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGAtttt                                                                                 <  2:3328215/148‑1 (MQ=255)
                                                                    aTTACCAATTTCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTc                                                                                <  1:75630/148‑1 (MQ=255)
                                                                    aTTACCAATTTCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTc                                                                                >  2:1882485/1‑148 (MQ=255)
                                                                    aTTACCAATTTCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCAGGAGGCGCGACGGAACCAATCACACCAGGGATTTTc                                                                                >  2:2771658/1‑148 (MQ=255)
                                                                     ttACCAATTTCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCa                                                                               <  1:3118322/148‑1 (MQ=255)
                                                                     ttACCAATTTCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCa                                                                               <  2:8715/148‑1 (MQ=255)
                                                                       aCCAATTTCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACt                                                                             >  2:3341493/1‑148 (MQ=255)
                                                                        ccAATTTCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTc                                                                            >  2:1393693/1‑148 (MQ=255)
                                                                         cAATTTCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTcc                                                                           <  2:1971802/148‑1 (MQ=255)
                                                                         cAATTTCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTcc                                                                           >  1:2442429/1‑148 (MQ=255)
                                                                          aaTTTCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTccc                                                                          <  2:1688716/148‑1 (MQ=255)
                                                                            tttCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCtt                                                                        <  1:1393693/148‑1 (MQ=255)
                                                                              tCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTtgt                                                                      <  2:1238392/148‑1 (MQ=255)
                                                                                  cgccgcGCTTTTCCACCTCTTCGCTACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTtgtgtgt                                                                  >  1:1462137/1‑148 (MQ=255)
                                                                                   gccgcGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTtgtgtgtg                                                                 <  1:1345986/148‑1 (MQ=255)
                                                                                     cgcgCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCg                                                               >  2:968796/1‑148 (MQ=255)
                                                                                      gcgcTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGt                                                              <  2:1694269/148‑1 (MQ=255)
                                                                                       cgcTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTa                                                             <  1:2076611/148‑1 (MQ=255)
                                                                                         cTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACCGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATg                                                           >  1:3454428/1‑148 (MQ=255)
                                                                                          ttttCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATgg                                                          <  1:899663/148‑1 (MQ=255)
                                                                                          ttttCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATgg                                                          >  1:788711/1‑148 (MQ=255)
                                                                                           tttCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGt                                                         >  2:1014913/1‑148 (MQ=255)
                                                                                           tttCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGt                                                         >  1:2837498/1‑148 (MQ=255)
                                                                                             tCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTa                                                       <  1:3314269/148‑1 (MQ=255)
                                                                                              ccACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTAc                                                      >  1:3393695/1‑148 (MQ=255)
                                                                                               cACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGAGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTAcc                                                     <  1:2661584/148‑1 (MQ=255)
                                                                                                 ccTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTACCCg                                                   >  1:1310821/1‑148 (MQ=255)
                                                                                                   tctTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTACCCggt                                                 >  1:2208053/1‑148 (MQ=255)
                                                                                                    ctTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTGGTGTGTGCGTATGGTTACCCggtg                                                >  2:1561391/1‑148 (MQ=255)
                                                                                                        gcgACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTACCCGGTGGTTg                                            <  2:230665/148‑1 (MQ=255)
                                                                                                         cgACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTACCCGGTGGTTGa                                           <  2:3474015/148‑1 (MQ=255)
                                                                                                           aCTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTACCCGGTGGTTGAGc                                         >  2:782499/1‑148 (MQ=255)
                                                                                                           aCTGTTCGCGCCAATATCAATGAACATCGCATCAAATGCCAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTACCCGGTGGTTGAGc                                         <  2:2787075/148‑1 (MQ=255)
                                                                                                            cTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTACCCGGTGGTTGAGCa                                        >  2:3363777/1‑148 (MQ=255)
                                                                                                            cTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCACGGATTTTCACTCCCATGTGTGTGCGTATGGTTAGACGGTGGTTGAGCa                                        >  1:299379/1‑148 (MQ=255)
                                                                                                             tGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTACCCGGTGGTTGAGCAt                                       >  2:560095/1‑148 (MQ=255)
                                                                                                                 cgcgCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTACCCGGTGGTTGAGCATCGAc                                   <  2:2276258/148‑1 (MQ=255)
                                                                                                                 cgcgCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTACCCGGTGGTTGAGCATCGAc                                   <  1:138461/148‑1 (MQ=255)
                                                                                                                     ccAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTACCCGGTGGTTGAGCATCGACTGAt                               >  2:1542634/1‑148 (MQ=255)
                                                                                                                      cAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTACCCGGTGGTTGAGCATCGACTGAtt                              <  2:182134/148‑1 (MQ=255)
                                                                                                                         tatCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTGTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTACCCGGTGGTTGAGCATCGACTGATTcca                           <  2:2863757/148‑1 (MQ=255)
                                                                                                                          atCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTACCCGGTGGTTGAGCATCGACTGATTccac                          >  2:23146/1‑148 (MQ=255)
                                                                                                                          atCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTACCCGGTGGTTGAGCATCGACTGATTccac                          >  2:935159/1‑148 (MQ=255)
                                                                                                                          atCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTACCCGGTGGTTGAGCATCGACTGATTccac                          <  2:471460/148‑1 (MQ=255)
                                                                                                                           tCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTACCCGGTGGTTGAGCATCGACTGATTccacc                         <  1:1606849/148‑1 (MQ=255)
                                                                                                                            cAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTACCCGGTGGTTGAGCATCGACTGATTccacca                        >  2:650944/1‑148 (MQ=255)
                                                                                                                                 aaCATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTACCCGGTGGTTGAGCATCGACTGATTCCACCAgccgc                   <  1:587181/148‑1 (MQ=255)
                                                                                                                                    aTCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTACCCGGTGGTTGAGCATCGACTGATTCCACCAGCCGCCaa                >  2:1501110/1‑148 (MQ=255)
                                                                                                                                      ctcATCAAATGACCGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTACCCGGTGGTTGAGCATCGACTGATTCCACCAGCCGCCAAtg              <  2:2156518/148‑1 (MQ=255)
                                                                                                                                      ctcATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTACCCGGTGGTTGAGCATCGACTGATTCCACCAGCCGCCAAtg              >  1:2665328/1‑148 (MQ=255)
                                                                                                                                        catcaAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTACCCGGTGGTTGAGCATCGACTGATTCCACCAGCCGCCAAtggt            >  2:1189364/1‑148 (MQ=255)
                                                                                                                                         atcaAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTACCCGGTGGTTGAGCATCGACTGATTCCACCAGCCGCCAAtggtg           >  2:2206575/1‑148 (MQ=255)
                                                                                                                                           caAATGACAGCGGTTGTTGCTTGTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTACCCGGTGGTTGAGCATCGACTGATTCCACCAGCCGCCAATggtggt         <  2:165276/148‑1 (MQ=255)
                                                                                                                                              aTGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTACCCGGTGGTTGAGCATCGACTGATTCCACCAGCCGCCAATGGTGGTaaa      >  2:2679743/1‑148 (MQ=255)
                                                                                                                                               tGACAGCGGTTGTTGCATTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTACCCGGTGGTTGAGCATCGACTGATTCCACCAGCCGCCAATGGTGGTaaaa     <  2:2984784/148‑1 (MQ=255)
                                                                                                                                                gACCGCGGTTGTTGCTTTTTCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTACCCGGTGGTTGAGCATCGACTGATTCCACCAGCCGCCAATGGTGGTAAAAc    >  1:2141016/1‑148 (MQ=255)
                                                                                                                                                 aCAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTACCCGGTGGTTGAGCATCGACTGATTCCACCAGCCGCCAATGGTGGTAAAACg   <  2:3398940/148‑1 (MQ=255)
                                                                                                                                                  cAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTACCCGGTGGTTGAGCATCGACTGATTCCACCAGCCGCCAATGGTGGTAAAACGc  <  2:1448095/148‑1 (MQ=255)
                                                                                                                                                  cAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTACCCGGTGGTTGAGCATCGACTGATTCCACCAGCCGCCAATGGTGGTAAAACGc  <  1:83218/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TTATCCAACGCCTTGCCGACCACTTTATCTTCGCCCCAGCAGGCAAAATTGGCTTCCGGGCTAATAAAATTACCAATTTCCACGCCGCGCTTTTCCACCTCTTCGCGACTGTTCGCGCCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTACCCGGTGGTTGAGCATCGACTGATTCCACCAGCCGCCAATGGTGGTAAAACGC  >  CP009273/4083759‑4084052

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑