Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,152,819 T→G 6.7% K127T (AAG→ACG)  trmA ← tRNA m(5)U54 methyltransferase, SAM‑dependent; tmRNA m(5)U341 methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,152,8190TG 6.7% 629.4 / 33.5 229K127T (AAG→ACG) trmAtRNA m(5)U54 methyltransferase, SAM‑dependent; tmRNA m(5)U341 methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (107/106);  new base G (10/6);  total (117/112)
Fisher's exact test for biased strand distribution p-value = 4.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 239 total aligned reads displayed.

CCTGCGACCGGCAGACGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGATTATTACGCACACCCGCAATCATCGCCGTCATCAACTGGTTGATAAGTTCACTGGCGGCGG  >  CP009273/4152672‑4152966
                                                                                                                                                   |                                                                                                                                                   
 cTGCGACCGGCAGACGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGctt                                                                                                                                                    <  1:2550837/148‑1 (MQ=255)
 cTGCGACCGGCAGACGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGctt                                                                                                                                                    <  1:765451/148‑1 (MQ=255)
   gCGACCGGCAGACGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGcttct                                                                                                                                                  <  1:2434975/148‑1 (MQ=255)
    cGACCGGCAGACGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGcttctt                                                                                                                                                 <  2:1888807/148‑1 (MQ=255)
        cGGCAGACGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATgg                                                                                                                                             <  2:1200225/148‑1 (MQ=255)
           cAGACGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGtat                                                                                                                                          >  2:3546685/1‑148 (MQ=255)
              aCGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGTTATAGc                                                                                                                                       >  1:2685926/1‑148 (MQ=255)
              aCGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGc                                                                                                                                       <  1:115092/148‑1 (MQ=255)
               cGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCa                                                                                                                                      <  1:987734/148‑1 (MQ=255)
                  tCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAggg                                                                                                                                   >  2:2660587/1‑148 (MQ=255)
                  tCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCGTCTTATGGTATAGCAggg                                                                                                                                   >  2:2625818/1‑148 (MQ=255)
                      cGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAac                                                                                                                               >  2:2573650/1‑148 (MQ=255)
                        aTGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAacca                                                                                                                             <  1:3374728/148‑1 (MQ=255)
                            aaTCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGc                                                                                                                         >  1:1857432/1‑148 (MQ=255)
                             aTCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGcc                                                                                                                        <  1:1875054/148‑1 (MQ=255)
                             aTCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGcc                                                                                                                        <  2:1311489/148‑1 (MQ=255)
                             aTCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGcc                                                                                                                        <  1:2618705/148‑1 (MQ=255)
                             aTCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGcc                                                                                                                        >  1:652906/1‑148 (MQ=255)
                              tCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCt                                                                                                                       >  2:165947/1‑148 (MQ=255)
                              tCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCt                                                                                                                       >  1:809101/1‑148 (MQ=255)
                               ccTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCCGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTg                                                                                                                      <  2:2155462/148‑1 (MQ=255)
                               ccTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTg                                                                                                                      <  2:1582662/148‑1 (MQ=255)
                               ccTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTg                                                                                                                      <  2:395123/148‑1 (MQ=255)
                                 tGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGAt                                                                                                                    <  1:2996784/148‑1 (MQ=255)
                                 tGAACCAGCTAGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGAATCGCGCAGGCCCTCCTCTTACTGACGCCACTCATCATCCAGCTTCTTATGGTCTAGAAGGGAAACCACCGCATGAt                                                                                                                    >  2:2961392/1‑148 (MQ=255)
                                     ccAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTAGAGCAGGGAAACCACCGCCTGATTACt                                                                                                                <  2:1840578/148‑1 (MQ=255)
                                       aGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCa                                                                                                              <  2:2887716/148‑1 (MQ=255)
                                        gCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAg                                                                                                             <  1:1202266/148‑1 (MQ=255)
                                        gCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAg                                                                                                             >  2:3097608/1‑148 (MQ=255)
                                         cTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAgt                                                                                                            >  1:3068669/1‑148 (MQ=255)
                                           cGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAgtgt                                                                                                          >  1:997604/1‑148 (MQ=255)
                                             aTTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAg                                                                                                        >  1:2825613/1‑148 (MQ=255)
                                              ttttGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGt                                                                                                       <  1:1763551/148‑1 (MQ=255)
                                               tttGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTg                                                                                                      >  1:2121459/1‑148 (MQ=255)
                                               tttGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTg                                                                                                      >  2:2156327/1‑148 (MQ=255)
                                                ttGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGa                                                                                                     <  1:454756/148‑1 (MQ=255)
                                                ttGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGa                                                                                                     <  1:2377727/148‑1 (MQ=255)
                                                ttGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCGTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGa                                                                                                     >  2:2818190/1‑148 (MQ=255)
                                                    ttttCGTTGCCCGACCAATCAGATGCACAGTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCGTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTa                                                                                                 >  2:463523/1‑148 (MQ=255)
                                                     tttCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTaa                                                                                                <  1:2919576/148‑1 (MQ=255)
                                                         gTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTaatcaa                                                                                            <  1:2258142/148‑1 (MQ=255)
                                                          ttGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTaatcaat                                                                                           <  2:782190/148‑1 (MQ=255)
                                                                gACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGaa                                                                                     >  2:1672801/1‑148 (MQ=255)
                                                                  ccAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGaaca                                                                                   >  1:155385/1‑148 (MQ=255)
                                                                  ccAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCGTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGaaca                                                                                   >  1:1711228/1‑148 (MQ=255)
                                                                      tCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACtt                                                                               >  2:2489575/1‑148 (MQ=255)
                                                                      tCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACtt                                                                               >  1:1072268/1‑148 (MQ=255)
                                                                      tCAGATGCACATTCAGAGTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACtt                                                                               <  2:1294123/148‑1 (MQ=255)
                                                                       cAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTtg                                                                              >  1:1782355/1‑148 (MQ=255)
                                                                          aTGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTgg                                                                           >  2:1979421/1‑148 (MQ=255)
                                                                             cacaTTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGgcgc                                                                        >  2:365993/1‑148 (MQ=255)
                                                                             cacaTTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGgcgc                                                                        <  1:3398946/148‑1 (MQ=255)
                                                                               caTTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAg                                                                      >  2:631406/1‑148 (MQ=255)
                                                                                aTTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGa                                                                     <  1:2246592/148‑1 (MQ=255)
                                                                                    aGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACgg                                                                 <  2:176951/148‑1 (MQ=255)
                                                                                     gATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACggg                                                                >  1:2304702/1‑148 (MQ=255)
                                                                                         cTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGatta                                                            >  1:1273998/1‑148 (MQ=255)
                                                                                         cTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGatta                                                            <  2:2426194/148‑1 (MQ=255)
                                                                                         cTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGatta                                                            <  2:169457/148‑1 (MQ=255)
                                                                                          tGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGattat                                                           <  2:3315034/148‑1 (MQ=255)
                                                                                          tGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGattat                                                           <  2:3314847/148‑1 (MQ=255)
                                                                                          tGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGattat                                                           <  1:1733362/148‑1 (MQ=255)
                                                                                           gcgcgcgcAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGattatt                                                          <  1:736973/148‑1 (MQ=255)
                                                                                           gcgcgcgcAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGattatt                                                          <  2:2280517/148‑1 (MQ=255)
                                                                                               gcgcAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGATTATTACGc                                                      <  2:1043759/148‑1 (MQ=255)
                                                                                                cgcAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGATTATTACGca                                                     >  2:1369483/1‑148 (MQ=255)
                                                                                                 gcAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGATTATTACGcac                                                    <  2:824300/148‑1 (MQ=255)
                                                                                                    gTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGATTATTACGCACAcc                                                 >  2:3412097/1‑148 (MQ=255)
                                                                                                     tGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGATTATTACGCACAccc                                                >  1:3419242/1‑148 (MQ=255)
                                                                                                      gCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGATTATTACGCACACCCg                                               >  2:221418/1‑148 (MQ=255)
                                                                                                      gCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCGTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGATTATTACGCACACCCg                                               >  2:543478/1‑148 (MQ=255)
                                                                                                        aTCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGATTATTACGCACACCCGCa                                             <  1:1156623/148‑1 (MQ=255)
                                                                                                         tCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGATTATTACGCACACCCGCaa                                            <  1:2553786/148‑1 (MQ=255)
                                                                                                         tCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGATTATTACGCACACCCGCaa                                            >  2:2796635/1‑148 (MQ=255)
                                                                                                           gcgcAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGATTATTACGCACACCCGCAatc                                          <  2:726300/148‑1 (MQ=255)
                                                                                                                 ggCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGATTATTACGCACACCCGCAATCATCGcc                                    >  1:1433509/1‑148 (MQ=255)
                                                                                                                  gCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGATTATTACGCACACCCGCAATCATCGCCg                                   >  2:3062199/1‑148 (MQ=255)
                                                                                                                         cTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGATTATTACGCACACCCGCAATCATCGCCGTCATCaa                            <  1:1672801/148‑1 (MQ=255)
                                                                                                                           tCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGATTATTACGCACACCCGCAATCATCGCCGTCATCAACt                          <  1:1979421/148‑1 (MQ=255)
                                                                                                                              tGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGATTATTACGCACACCCGCAATCATCGCCGTCATCAACTGGt                       <  2:2763127/148‑1 (MQ=255)
                                                                                                                              tGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGATTATTACGCACACCCGCAATCATCGCCGTCATCAACTGGt                       >  2:2977257/1‑148 (MQ=255)
                                                                                                                              tGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGATTATTACGCACACCCGCAATCATCGCCGTCATCAACTGGt                       >  1:2063124/1‑148 (MQ=255)
                                                                                                                              tGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGATTATTACGCACACCCGCAATCATCGCCGTCATCAACTGGt                       >  1:2162178/1‑148 (MQ=255)
                                                                                                                              tGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGATTATTACGCACACCCGCAATCATCGCCGTCATCAACTGGt                       >  1:2280598/1‑148 (MQ=255)
                                                                                                                                aCGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGATTATTACGCACACCCGCAATCATCGCCGTCATCAACTGGTTg                     <  2:1130311/148‑1 (MQ=255)
                                                                                                                                  gCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGATTATTACGCACACCCGCAATCATCGCCGTCATCAACTGGTTGAt                   <  1:226327/148‑1 (MQ=255)
                                                                                                                                    cACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGATTATTACGCACACCCGCAATCATCGCCGTCATCAACTGGTTGATaa                 <  1:2791746/148‑1 (MQ=255)
                                                                                                                                     aCTCATCATCCAGCGTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGATTATTACGCACACCCGCAATCATCGCCGTCATCAACTGGTTGATAAg                <  1:3099175/148‑1 (MQ=255)
                                                                                                                                      cTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGATTATTACGCACACCCGCAATCATCGCCGTCATCAACTGGTTGATAAGt               >  1:1349494/1‑148 (MQ=255)
                                                                                                                                        catcatCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGATTATTACGCACACCCGCAATCATCGCCGTCATCAACTGGTTGATAAGTTc             >  1:3004594/1‑148 (MQ=255)
                                                                                                                                         atcatcCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGATTATTACGCACACCCGCAATCATCGCCGTCATCAACTGGTTGATAAGTTCa            <  1:3074464/148‑1 (MQ=255)
                                                                                                                                         atcatcCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGATTATTACGCACACCCGCAATCATCGCCGTCATCAACTGGTTGATAAGTTCa            <  2:200941/148‑1 (MQ=255)
                                                                                                                                         atcatcCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGATTATTACGCACACCCGCAATCATCGCCGTCATCAACTGGTTGATAAGTTCa            <  2:1522678/148‑1 (MQ=255)
                                                                                                                                         atcatcCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGATTATTACGCACACCCGCAATCATCGCCGTCATCAACTGGTTGATAAGTTCa            >  2:2907355/1‑148 (MQ=255)
                                                                                                                                          tcatcCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGATTATTACGCACACCCGCAATCATCGCCGTCATCAACTGGTTGATAAGTTCAc           >  2:3538999/1‑148 (MQ=255)
                                                                                                                                          tcatcCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTAGCGCAGAACGGGATTATTACGCACACCCGCAATCATCGCCGTCATCAACTGGTTGATAAGTTCAc           <  1:3546685/148‑1 (MQ=255)
                                                                                                                                               cAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGATTATTACGCACACCCGCAATCATCGCCGTCATCAACTGGTTGATAAGTTCACTggcg      <  1:2587951/148‑1 (MQ=255)
                                                                                                                                                 gCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGATTATTACGCACACCCGCAATCATCGCCGTCATCAACTGGTTGATAAGTTCACTggcggc    >  1:486161/1‑148 (MQ=255)
                                                                                                                                                  cttcttATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGATTATTACGCACACCCGCAATCATCGCCGTCATCAACTGGTTGATAAGTTCACTGgcggcg   >  2:577703/1‑148 (MQ=255)
                                                                                                                                                   ttcttATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGATTATTACGCACACCCGCAATCATCGCCGTCATCAACTGGTTGATAAGTTCACTGGcggcgg  <  2:38918/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CCTGCGACCGGCAGACGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGATTATTACGCACACCCGCAATCATCGCCGTCATCAACTGGTTGATAAGTTCACTGGCGGCGG  >  CP009273/4152672‑4152966

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑