Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,176,823 G→T 100% D516Y (GAC→TAC)  rpoC → RNA polymerase, beta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,176,8230GT100.0% 659.9 / NA 199D516Y (GAC→TAC) rpoCRNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (79/120);  total (79/120)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 199 total aligned reads displayed.

TGTTCACGTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTGACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAG  >  CP009273/4176678‑4176967
                                                                                                                                                 |                                                                                                                                                
  ttCACGTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACtg                                                                                                                                              <  1:263255/148‑1 (MQ=255)
    cACGTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACtgtg                                                                                                                                            <  1:765569/148‑1 (MQ=255)
       gTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTa                                                                                                                                         <  1:2560001/148‑1 (MQ=255)
       gTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTa                                                                                                                                         <  2:3466899/148‑1 (MQ=255)
        tACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTaa                                                                                                                                        <  2:2545131/148‑1 (MQ=255)
        tACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTaa                                                                                                                                        <  2:2955062/148‑1 (MQ=255)
           cGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGc                                                                                                                                     <  1:1797969/148‑1 (MQ=255)
                 cGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAgg                                                                                                                               <  1:2496070/148‑1 (MQ=255)
                    tGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGa                                                                                                                            <  1:534617/148‑1 (MQ=255)
                     ggAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGaa                                                                                                                           <  2:3028168/148‑1 (MQ=255)
                     ggAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGaa                                                                                                                           >  1:777040/1‑148 (MQ=255)
                       aaGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAgg                                                                                                                         <  1:1521763/148‑1 (MQ=255)
                        aGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGc                                                                                                                        >  2:1205020/1‑148 (MQ=255)
                        aGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGc                                                                                                                        >  2:1156348/1‑148 (MQ=255)
                        aGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGc                                                                                                                        <  1:3301802/148‑1 (MQ=255)
                        aGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGc                                                                                                                        <  2:2350618/148‑1 (MQ=255)
                          cccAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCAt                                                                                                                      <  1:2808411/148‑1 (MQ=255)
                            cAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATgg                                                                                                                    >  1:968863/1‑148 (MQ=255)
                            cAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATgg                                                                                                                    >  1:968760/1‑148 (MQ=255)
                                 ggAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTg                                                                                                               <  2:1577696/148‑1 (MQ=255)
                                  gAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCACTCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGa                                                                                                              <  2:1101929/148‑1 (MQ=255)
                                  gAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGa                                                                                                              <  2:477862/148‑1 (MQ=255)
                                  gAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGa                                                                                                              >  2:2955596/1‑148 (MQ=255)
                                  gAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGa                                                                                                              <  2:2266541/148‑1 (MQ=255)
                                   aaGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGAc                                                                                                             >  1:1482621/1‑148 (MQ=255)
                                   aaGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGAc                                                                                                             >  1:3336294/1‑148 (MQ=255)
                                    aGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTCGTACTGGGTCTGTACTACATGACCCGTTACTgtgt                                                                                                                                           <  1:2269897/117‑1 (MQ=255)
                                          tGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCg                                                                                                      >  1:3485757/1‑148 (MQ=255)
                                             gcTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGaaa                                                                                                   <  1:3437117/148‑1 (MQ=255)
                                                  tgatgTCTACCAACAACATCCTGTCCCCGTCGAACGGCGAACCACTCATCGTTCCGTCTCAGGACGTTGTACTGTGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGATGGCATGGTGCTGACTGGCCCGAAAGAAGc                                                                                              <  1:2111829/148‑1 (MQ=255)
                                                    atgTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAg                                                                                            <  1:654102/148‑1 (MQ=255)
                                                    atgTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAg                                                                                            >  2:421026/1‑148 (MQ=255)
                                                    atgTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAg                                                                                            >  2:422061/1‑148 (MQ=255)
                                                        cTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACg                                                                                        <  1:116779/148‑1 (MQ=255)
                                                         tACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGt                                                                                       <  1:1889690/148‑1 (MQ=255)
                                                          aCCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTc                                                                                      >  2:770147/1‑148 (MQ=255)
                                                          aCCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTc                                                                                      >  2:2487148/1‑148 (MQ=255)
                                                          aCCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTc                                                                                      >  2:2486634/1‑148 (MQ=255)
                                                            caacaaCATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTg                                                                                    >  2:2584292/1‑148 (MQ=255)
                                                             aacaacATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACTTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGt                                                                                   <  1:2388750/148‑1 (MQ=255)
                                                               caacaTCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTAt                                                                                 <  1:690384/148‑1 (MQ=255)
                                                                    tCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGctc                                                                            >  2:3415843/1‑148 (MQ=255)
                                                                     ccTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGctct                                                                           >  2:1433473/1‑148 (MQ=255)
                                                                        gTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCtctggt                                                                        <  2:585819/148‑1 (MQ=255)
                                                                        gTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGCCTGTATCGCtctggt                                                                        <  1:2667789/148‑1 (MQ=255)
                                                                         tCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCtctggtc                                                                       <  2:2056324/148‑1 (MQ=255)
                                                                          ccccGGCGAACGGCGAACCAATCCTCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCtctggtct                                                                      <  2:844118/148‑1 (MQ=255)
                                                                          ccccGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCtctggtct                                                                      <  1:3265627/148‑1 (MQ=255)
                                                                             cggcgaacggcgaaCCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGc                                                                   >  2:1019511/1‑148 (MQ=255)
                                                                             cggcgaacggcgaaCCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGc                                                                   >  2:704443/1‑148 (MQ=255)
                                                                             cggcgaacggcgaaCCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGc                                                                   <  2:3120859/148‑1 (MQ=255)
                                                                                cgaacggcgaacCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTtc                                                                <  1:1009620/148‑1 (MQ=255)
                                                                                 gaacggcgaacCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTtct                                                               <  1:413508/148‑1 (MQ=255)
                                                                                 gaacggcgaacCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTtct                                                               <  1:1704259/148‑1 (MQ=255)
                                                                                  aacggcgaacCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTtctc                                                              <  1:1316480/148‑1 (MQ=255)
                                                                                    cggcgaacCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTg                                                            >  2:1518567/1‑148 (MQ=255)
                                                                                    cggcgaacCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTg                                                            >  2:3282771/1‑148 (MQ=255)
                                                                                    cggcgaacCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTg                                                            <  1:878468/148‑1 (MQ=255)
                                                                                          acCACTCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATgcg                                                      <  1:108564/148‑1 (MQ=255)
                                                                                           ccAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATgcgc                                                     >  1:919862/1‑148 (MQ=255)
                                                                                              atcatcGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGcgcgcg                                                  >  1:2278183/1‑148 (MQ=255)
                                                                                              atcatcGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGcgcgcg                                                  >  2:3178236/1‑148 (MQ=255)
                                                                                              atcatcGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGcgcgcg                                                  <  1:2955596/148‑1 (MQ=255)
                                                                                               tcctcGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCATAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGt                                                 <  2:2754999/145‑1 (MQ=255)
                                                                                               tcatcGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGt                                                 <  2:2459661/148‑1 (MQ=255)
                                                                                                catcGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGtt                                                >  2:1855367/1‑148 (MQ=255)
                                                                                                      tCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTg                                          <  2:696577/148‑1 (MQ=255)
                                                                                                        cGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCg                                        <  2:1384426/148‑1 (MQ=255)
                                                                                                        cGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCg                                        <  2:3064449/148‑1 (MQ=255)
                                                                                                         gTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGt                                       <  2:1318259/148‑1 (MQ=255)
                                                                                                         gTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGt                                       <  2:1321934/148‑1 (MQ=255)
                                                                                                             cAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCa                                   >  2:903101/1‑148 (MQ=255)
                                                                                                               ggACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCAcc                                 >  1:3456458/1‑148 (MQ=255)
                                                                                                               ggACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCAcc                                 >  1:541843/1‑148 (MQ=255)
                                                                                                                gACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCg                                <  2:3004040/148‑1 (MQ=255)
                                                                                                                 aCGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGa                               <  2:677437/148‑1 (MQ=255)
                                                                                                                 aCGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGa                               >  2:2193140/1‑148 (MQ=255)
                                                                                                                  cGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAg                              <  2:1809955/148‑1 (MQ=255)
                                                                                                                  cGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAg                              <  1:666904/148‑1 (MQ=255)
                                                                                                                  cGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAg                              <  2:3511636/148‑1 (MQ=255)
                                                                                                                    ttGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTa                            <  1:1945471/148‑1 (MQ=255)
                                                                                                                     tGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTAt                           <  2:902589/148‑1 (MQ=255)
                                                                                                                        aCTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGaa                        >  2:3481158/1‑148 (MQ=255)
                                                                                                                        aCTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGaa                        <  1:2871308/148‑1 (MQ=255)
                                                                                                                          tGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGaaaa                      <  1:2418526/148‑1 (MQ=255)
                                                                                                                           gggTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGaaaaa                     <  2:2133934/148‑1 (MQ=255)
                                                                                                                           gggTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGaaaaa                     <  2:285886/148‑1 (MQ=255)
                                                                                                                             gTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGa                   <  2:1876929/148‑1 (MQ=255)
                                                                                                                                 gTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCt               >  2:1013097/1‑148 (MQ=255)
                                                                                                                                  tactacATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTGAAAGTGCGTATCACCGAGTATGAAAAAGATGCTa              <  2:3098043/148‑1 (MQ=255)
                                                                                                                                   actacATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTaa             <  2:2272956/148‑1 (MQ=255)
                                                                                                                                   actacATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTaa             <  2:2403442/148‑1 (MQ=255)
                                                                                                                                    ctacATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAAc            <  1:1991273/148‑1 (MQ=255)
                                                                                                                                         tGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGa       >  1:2046912/1‑148 (MQ=255)
                                                                                                                                         tGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGa       <  2:968760/148‑1 (MQ=255)
                                                                                                                                         tGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGa       <  2:968863/148‑1 (MQ=255)
                                                                                                                                           aCCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAAt     >  1:1318001/1‑148 (MQ=255)
                                                                                                                                             ccGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATta   <  2:1071611/148‑1 (MQ=255)
                                                                                                                                              cGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATtag  <  1:2309334/148‑1 (MQ=255)
                                                                                                                                              cGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATtag  >  2:3333100/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TGTTCACGTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTGACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAG  >  CP009273/4176678‑4176967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑