Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 40,957 C→A 100% V325V (GTG→GTT)  caiT ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927340,9570CA98.9% 579.7 / ‑1.8 177V325V (GTG→GTT) caiTputative transporter
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (72/103);  total (73/104)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 192 total aligned reads displayed.

TCAACAGCAGAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCATGATGAAGCTGGCACC  >  CP009273/40812‑41100
                                                                                                                                                 |                                                                                                                                               
tcAACAGCAGAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATaac                                                                                                                                               >  1:24072/1‑148 (MQ=255)
 cAACAGCAGAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATaacc                                                                                                                                              >  2:3433231/1‑148 (MQ=255)
   aCAGCAGAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATaaccca                                                                                                                                            >  1:304834/1‑148 (MQ=255)
   aCAGCAGAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATaaccca                                                                                                                                            >  2:1630092/1‑148 (MQ=255)
   aCAGCAGAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATaaccca                                                                                                                                            <  1:1338605/148‑1 (MQ=255)
     agcagAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATaacccacc                                                                                                                                          <  1:2036553/148‑1 (MQ=255)
      gcagAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACccacca                                                                                                                                         >  1:1981864/1‑148 (MQ=255)
        agagTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATg                                                                                                                                       <  2:654794/148‑1 (MQ=255)
          agTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGcc                                                                                                                                     >  1:225826/1‑148 (MQ=255)
           gtgtTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGccc                                                                                                                                    >  2:2519057/1‑148 (MQ=255)
           gtgtTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGccc                                                                                                                                    <  1:934254/148‑1 (MQ=255)
           gtgtTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGccc                                                                                                                                    <  1:2711424/148‑1 (MQ=255)
               tactacCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTa                                                                                                                                >  1:2848314/1‑148 (MQ=255)
                actacCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAg                                                                                                                               >  2:2552165/1‑148 (MQ=255)
                  tacCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGCGATGCGGGCGAGGACGATACTCATCTGGATCGCATAAATAACCCACCATGCCCAGTAGaa                                                                                                                             >  2:1954958/1‑148 (MQ=255)
                  tacCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGaa                                                                                                                             <  2:3028336/148‑1 (MQ=255)
                    ccGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAaca                                                                                                                           >  1:827093/1‑148 (MQ=255)
                     cGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAacac                                                                                                                          >  1:2882725/1‑148 (MQ=255)
                      gAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACg                                                                                                                         <  2:3225510/148‑1 (MQ=255)
                       aGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACgg                                                                                                                        <  1:3097528/148‑1 (MQ=255)
                       aGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACgg                                                                                                                        >  1:3034609/1‑148 (MQ=255)
                        gTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGt                                                                                                                       >  2:705510/1‑148 (MQ=255)
                            aGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAg                                                                                                                   >  2:2027327/1‑148 (MQ=255)
                               ccACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGccc                                                                                                                <  1:3433231/148‑1 (MQ=255)
                                      aTCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGa                                                                                                         >  2:182369/1‑148 (MQ=255)
                                        ccAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAg                                                                                                       <  1:375867/148‑1 (MQ=255)
                                        ccAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAg                                                                                                       <  1:2044764/148‑1 (MQ=255)
                                           ggTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAgccg                                                                                                    <  1:1290074/148‑1 (MQ=255)
                                             ttGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAgccgcc                                                                                                  >  1:1839542/1‑148 (MQ=255)
                                                 cGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTa                                                                                              <  1:2930113/148‑1 (MQ=255)
                                                  gCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAg                                                                                             <  2:2503835/148‑1 (MQ=255)
                                                   cTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGc                                                                                            >  2:306404/1‑148 (MQ=255)
                                                    tGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCg                                                                                           >  1:3111049/1‑148 (MQ=255)
                                                      tCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGAt                                                                                         >  1:244176/1‑148 (MQ=255)
                                                        aGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATgg                                                                                       <  2:1581935/148‑1 (MQ=255)
                                                          ccccAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGa                                                                                     <  1:2116755/148‑1 (MQ=255)
                                                          ccccAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGa                                                                                     <  1:2139834/148‑1 (MQ=255)
                                                          ccccAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGa                                                                                     <  1:1790160/148‑1 (MQ=255)
                                                          ccccAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGa                                                                                     <  1:2411319/148‑1 (MQ=255)
                                                           cccAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGAt                                                                                    <  1:935817/148‑1 (MQ=255)
                                                           cccAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGAt                                                                                    <  1:935070/148‑1 (MQ=255)
                                                            ccAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATc                                                                                   <  2:1309450/148‑1 (MQ=255)
                                                             cAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCg                                                                                  <  2:2010181/148‑1 (MQ=255)
                                                             cAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCg                                                                                  <  2:2011478/148‑1 (MQ=255)
                                                                 aCCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGtat                                                                              <  2:1859580/148‑1 (MQ=255)
                                                                  ccATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGtata                                                                             >  1:2955764/1‑148 (MQ=255)
                                                                    aTGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGa                                                                           <  2:2292254/148‑1 (MQ=255)
                                                                    aTGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGa                                                                           <  2:2292272/148‑1 (MQ=255)
                                                                         gAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGaacaac                                                                      <  2:3494037/148‑1 (MQ=255)
                                                                         gAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGaacaac                                                                      <  1:1092628/148‑1 (MQ=255)
                                                                         gAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGaacaac                                                                      <  1:2399756/148‑1 (MQ=255)
                                                                           aGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACAt                                                                    <  1:1059049/148‑1 (MQ=255)
                                                                               caGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATgcgc                                                                <  2:2943239/148‑1 (MQ=255)
                                                                                 gTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCgg                                                              <  2:537975/148‑1 (MQ=255)
                                                                                   tCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCa                                                            <  2:2190273/148‑1 (MQ=255)
                                                                                     aCGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGa                                                          <  2:509651/148‑1 (MQ=255)
                                                                                      cGCACAGTACGACCACGCGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACCACATGCGCTGCAGAt                                                         <  2:524686/148‑1 (MQ=255)
                                                                                         acaGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACa                                                      <  2:3316161/148‑1 (MQ=255)
                                                                                           aGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATc                                                    <  1:462003/148‑1 (MQ=255)
                                                                                            gTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCa                                                   <  2:1909750/148‑1 (MQ=255)
                                                                                            gTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCa                                                   <  2:1911273/148‑1 (MQ=255)
                                                                                            gTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCa                                                   <  2:112865/148‑1 (MQ=255)
                                                                                            gTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCa                                                   <  2:3000124/148‑1 (MQ=255)
                                                                                             tACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAg                                                  >  2:2847135/1‑148 (MQ=255)
                                                                                              aCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGc                                                 >  1:2209975/1‑148 (MQ=255)
                                                                                                 aCCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAAc                                              >  2:165191/1‑148 (MQ=255)
                                                                                                 aCCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAAc                                              <  1:1180090/148‑1 (MQ=255)
                                                                                                  ccACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACa                                             <  2:1036303/148‑1 (MQ=255)
                                                                                                   cACGGGAGATGTGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACAt                                            <  2:2376983/148‑1 (MQ=255)
                                                                                                     cGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATcc                                          <  2:584708/148‑1 (MQ=255)
                                                                                                     cGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATcc                                          <  2:1723274/148‑1 (MQ=255)
                                                                                                          gaTGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCAcc                                     >  2:1244458/1‑148 (MQ=255)
                                                                                                             gCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGaa                                  <  1:582219/148‑1 (MQ=255)
                                                                                                             gCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGaa                                  <  1:3273576/148‑1 (MQ=255)
                                                                                                              cGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAAt                                 <  1:705510/148‑1 (MQ=255)
                                                                                                              cGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAAt                                 <  2:24072/148‑1 (MQ=255)
                                                                                                                     ggAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGaa                          >  2:3296066/1‑148 (MQ=255)
                                                                                                                     ggAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGaa                          >  1:508834/1‑148 (MQ=255)
                                                                                                                       aaGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAagt                        >  1:3198265/1‑148 (MQ=255)
                                                                                                                         gATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAagtag                      >  1:1140214/1‑148 (MQ=255)
                                                                                                                           tACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGtt                    <  1:1098858/148‑1 (MQ=255)
                                                                                                                            aCTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTc                   <  1:1463371/148‑1 (MQ=255)
                                                                                                                            aCTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTc                   <  1:200603/148‑1 (MQ=255)
                                                                                                                            aCTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTc                   >  1:1008655/1‑148 (MQ=255)
                                                                                                                              tCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCat                 >  2:3062923/1‑148 (MQ=255)
                                                                                                                              tCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCat                 >  1:2397130/1‑148 (MQ=255)
                                                                                                                              tCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCat                 >  1:905755/1‑148 (MQ=255)
                                                                                                                              tCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCat                 >  1:722088/1‑148 (MQ=255)
                                                                                                                              tCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCGTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCat                 >  1:1697853/1‑148 (MQ=255)
                                                                                                                               cATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCatg                >  2:2739163/1‑148 (MQ=255)
                                                                                                                                 tCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCatgat              <  2:2474012/148‑1 (MQ=255)
                                                                                                                                  cTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCatgatg             >  2:3067151/1‑148 (MQ=255)
                                                                                                                                     gATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCATGATGAAg          <  1:1071389/148‑1 (MQ=255)
                                                                                                                                     gATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCATGATGAAg          >  1:1926525/1‑148 (MQ=255)
                                                                                                                                     gATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCATGATGAAg          <  1:2511659/148‑1 (MQ=255)
                                                                                                                                       tAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCATGATGAAGCt        >  2:210770/1‑148 (MQ=255)
                                                                                                                                        aGCATAAATAACCCACCATGCCCCGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCATGATGAAGCTg       <  2:222036/148‑1 (MQ=255)
                                                                                                                                         gCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCATGATGAAGCTgg      >  1:1695748/1‑148 (MQ=255)
                                                                                                                                         gCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCATGATGAAGCTgg      >  1:3389596/1‑148 (MQ=255)
                                                                                                                                           ataaataaCCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCATGATGAAGCTGGCa    >  2:1799526/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
TCAACAGCAGAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCATGATGAAGCTGGCACC  >  CP009273/40812‑41100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑