Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,274,789 G→T 5.2% F14L (TTC→TTA)  actP ← acetate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,274,7890GT 5.2% 1023.9 / 18.0 265F14L (TTC→TTA) actPacetate transporter
Reads supporting (aligned to +/- strand):  ref base G (127/124);  new base T (7/7);  total (134/131)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 267 total aligned reads displayed.

TAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACAATCACTCCAA  >  CP009273/4274643‑4274936
                                                                                                                                                  |                                                                                                                                                   
 agtCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGaa                                                                                                                                                   <  1:3296605/148‑1 (MQ=255)
 agtCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGaa                                                                                                                                                   <  1:1973647/148‑1 (MQ=255)
 agtCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGaa                                                                                                                                                   >  1:1853533/1‑148 (MQ=255)
   tCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAg                                                                                                                                                 <  1:1222772/148‑1 (MQ=255)
   tCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAg                                                                                                                                                 >  2:554987/1‑148 (MQ=255)
   tCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAg                                                                                                                                                 >  1:1574715/1‑148 (MQ=255)
     gCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCTAAAggg                                                                                                                                               <  1:1509084/148‑1 (MQ=255)
      cTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGa                                                                                                                                              >  1:2883029/1‑148 (MQ=255)
      cTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGa                                                                                                                                              >  2:173056/1‑148 (MQ=255)
       tACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAg                                                                                                                                             <  1:2056036/148‑1 (MQ=255)
        aCGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCTAAAGGGAgt                                                                                                                                            >  1:2543748/1‑148 (MQ=255)
        aCGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAgt                                                                                                                                            >  2:1423276/1‑148 (MQ=255)
          gagaACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAgtgt                                                                                                                                          <  2:834148/148‑1 (MQ=255)
            gaACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTgg                                                                                                                                        <  1:43253/148‑1 (MQ=255)
             aaCGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTggc                                                                                                                                       >  2:584912/1‑148 (MQ=255)
               cGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTggcgg                                                                                                                                     <  1:1683502/148‑1 (MQ=255)
                  aCGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCaa                                                                                                                                  <  2:1800896/148‑1 (MQ=255)
                  aCGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCaa                                                                                                                                  >  2:1720252/1‑148 (MQ=255)
                       ttttGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGcc                                                                                                                             >  1:3365617/1‑148 (MQ=255)
                       ttttGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGcc                                                                                                                             >  1:88874/1‑148 (MQ=255)
                           gATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCa                                                                                                                         <  2:1167542/148‑1 (MQ=255)
                           gATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCa                                                                                                                         >  2:1243900/1‑148 (MQ=255)
                              gCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGaa                                                                                                                      <  2:2216868/148‑1 (MQ=255)
                                     aGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCTAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCtttt                                                                                                               >  2:2667321/1‑148 (MQ=255)
                                       gTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCa                                                                                                             >  2:730646/1‑148 (MQ=255)
                                       gTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTAGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCa                                                                                                             <  2:399090/148‑1 (MQ=255)
                                          aTGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCatga                                                                                                          >  2:1291653/1‑148 (MQ=255)
                                           tGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCatgat                                                                                                         >  1:3331995/1‑148 (MQ=255)
                                             ccGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGc                                                                                                       >  1:3245400/1‑148 (MQ=255)
                                             ccGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGc                                                                                                       <  1:998788/148‑1 (MQ=255)
                                               gAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCtt                                                                                                     <  2:2724366/148‑1 (MQ=255)
                                                 gCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGt                                                                                                   >  1:1543033/1‑148 (MQ=255)
                                                      aaCACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTc                                                                                              <  1:1088864/148‑1 (MQ=255)
                                                       acacGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCa                                                                                             >  1:2316221/1‑148 (MQ=255)
                                                       acacGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCa                                                                                             >  2:70088/1‑148 (MQ=255)
                                                        cacGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCTAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCAt                                                                                            <  1:2442210/148‑1 (MQ=255)
                                                        cacGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCAt                                                                                            >  1:164777/1‑148 (MQ=255)
                                                             cgaAAATCAGGAACATAATACTCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAgg                                                                                       <  1:1915466/148‑1 (MQ=255)
                                                             cgaAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAgg                                                                                       <  1:1414266/148‑1 (MQ=255)
                                                                aaaTCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCCCCGTCAGAACTCTTTTCATGATGCTTGTAAATCATGCACGACt                                                                                    >  1:3000420/1‑148 (MQ=255)
                                                                 aaTCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACtt                                                                                   >  2:1968437/1‑148 (MQ=255)
                                                                   tCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCa                                                                                 >  2:1908704/1‑148 (MQ=255)
                                                                   tCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCa                                                                                 >  2:110854/1‑148 (MQ=255)
                                                                    cAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCat                                                                                <  1:1013138/148‑1 (MQ=255)
                                                                      ggAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCatta                                                                              <  1:1006084/148‑1 (MQ=255)
                                                                       gAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCattat                                                                             <  1:3521877/148‑1 (MQ=255)
                                                                       gAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCattat                                                                             <  2:1511365/148‑1 (MQ=255)
                                                                       gAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCattat                                                                             >  1:3066909/1‑148 (MQ=255)
                                                                             aatactCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGAc                                                                       <  2:3403733/148‑1 (MQ=255)
                                                                             aataatCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGAc                                                                       <  2:625453/148‑1 (MQ=255)
                                                                             aataatCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGAc                                                                       <  2:3234366/148‑1 (MQ=255)
                                                                              ataatCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCTAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACg                                                                      <  2:473267/148‑1 (MQ=255)
                                                                                aatCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGt                                                                    <  2:3178712/148‑1 (MQ=255)
                                                                                  tCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCg                                                                  >  2:2237839/1‑148 (MQ=255)
                                                                                    gCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGaa                                                                <  2:2797052/148‑1 (MQ=255)
                                                                                    gCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGaa                                                                <  2:1729910/148‑1 (MQ=255)
                                                                                     ccTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAAt                                                               >  1:1084015/1‑148 (MQ=255)
                                                                                      cTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAAtt                                                              >  1:159662/1‑148 (MQ=255)
                                                                                       tGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTc                                                             >  2:383433/1‑148 (MQ=255)
                                                                                        gCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCg                                                            >  1:2659572/1‑148 (MQ=255)
                                                                                             ttCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGtt                                                       >  1:2204251/1‑148 (MQ=255)
                                                                                             ttCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGtt                                                       <  2:2998697/148‑1 (MQ=255)
                                                                                             ttCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGtt                                                       <  2:2998607/148‑1 (MQ=255)
                                                                                                gTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTcgc                                                    <  1:701620/148‑1 (MQ=255)
                                                                                                        gcgcTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGa                                            <  1:819557/148‑1 (MQ=255)
                                                                                                          gcTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCTAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATg                                          >  1:342343/1‑148 (MQ=255)
                                                                                                          gcTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCTAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATg                                          >  1:342480/1‑148 (MQ=255)
                                                                                                           ctctACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGt                                         >  1:1738360/1‑148 (MQ=255)
                                                                                                           ctctACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGt                                         <  2:1912977/148‑1 (MQ=255)
                                                                                                            tctACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTa                                        <  1:2267547/148‑1 (MQ=255)
                                                                                                                cGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATa                                    >  2:2501008/1‑148 (MQ=255)
                                                                                                                   ccccGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCg                                 <  1:2411053/148‑1 (MQ=255)
                                                                                                                      cGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTg                              <  2:1083086/148‑1 (MQ=255)
                                                                                                                          aaTAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGaa                          >  1:859557/1‑148 (MQ=255)
                                                                                                                           aTAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAAc                         >  2:2177412/1‑148 (MQ=255)
                                                                                                                           aTAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAAc                         >  2:406990/1‑148 (MQ=255)
                                                                                                                            tAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACa                        >  2:3540587/1‑148 (MQ=255)
                                                                                                                               cATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAg                     <  1:1318548/148‑1 (MQ=255)
                                                                                                                               cATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAg                     >  1:3518245/1‑148 (MQ=255)
                                                                                                                               cATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAg                     <  1:1020486/148‑1 (MQ=255)
                                                                                                                                 tCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGga                   >  1:1274305/1‑148 (MQ=255)
                                                                                                                                 tCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGga                   >  1:2703972/1‑148 (MQ=255)
                                                                                                                                  ccGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGgag                  <  2:2181394/148‑1 (MQ=255)
                                                                                                                                   cgcgGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGgaga                 <  2:1084015/148‑1 (MQ=255)
                                                                                                                                   cgcgGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGgaga                 <  2:3086719/148‑1 (MQ=255)
                                                                                                                                    gcggcgTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGAt                <  1:2274817/148‑1 (MQ=255)
                                                                                                                                     cggcgTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATc               <  2:2316221/148‑1 (MQ=255)
                                                                                                                                     cggcgTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATc               <  2:882910/148‑1 (MQ=255)
                                                                                                                                      ggcgTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATca              <  1:180609/148‑1 (MQ=255)
                                                                                                                                      ggcgTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATca              <  1:1449070/148‑1 (MQ=255)
                                                                                                                                        cgTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATcacc            <  2:3331995/148‑2 (MQ=255)
                                                                                                                                        cgTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATcacc            >  2:1590249/1‑147 (MQ=255)
                                                                                                                                           tAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATc         >  1:15147/1‑148 (MQ=255)
                                                                                                                                              cTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACt      >  1:2103738/1‑148 (MQ=255)
                                                                                                                                              cTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACt      >  1:549588/1‑148 (MQ=255)
                                                                                                                                              cTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACt      <  1:801176/148‑1 (MQ=255)
                                                                                                                                               tGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTc     <  1:480900/148‑1 (MQ=255)
                                                                                                                                                gCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTcc    >  1:2145141/1‑148 (MQ=255)
                                                                                                                                                gCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTcc    >  1:1617218/1‑148 (MQ=255)
                                                                                                                                                  gAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCaa  <  1:496625/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACAATCACTCCAA  >  CP009273/4274643‑4274936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑