Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,275,142 C→A 100% intergenic (‑1/+199) yjcH ← / ← acs DUF485 family inner membrane protein/acetyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,275,1420CA100.0% 812.1 / NA 245intergenic (‑1/+199)yjcH/acsDUF485 family inner membrane protein/acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (130/115);  total (130/115)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 251 total aligned reads displayed.

CGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTG  >  CP009273/4274997‑4275286
                                                                                                                                                 |                                                                                                                                                
cGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATaac                                                                                                                                                >  2:3289554/1‑148 (MQ=255)
  ggCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAg                                                                                                                                              >  2:358772/1‑148 (MQ=255)
    cgcgAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGAt                                                                                                                                            <  2:884295/148‑1 (MQ=255)
    cgcgAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGAt                                                                                                                                            <  2:412604/148‑1 (MQ=255)
     gcgACGGCGAACAGAAAAAAAAACCCGATAAAAACTGCCAGCATAATACTCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGAtt                                                                                                                                           <  2:3121226/148‑1 (MQ=255)
           gCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCag                                                                                                                                     >  2:3473842/1‑148 (MQ=255)
             tataaGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCagag                                                                                                                                   <  2:1337970/144‑1 (MQ=255)
              aTCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAgg                                                                                                                                  >  2:201930/1‑148 (MQ=255)
              aTCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGACATTCATAACAGATTCTCCAGAgg                                                                                                                                  >  1:1825466/1‑148 (MQ=255)
               tCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGt                                                                                                                                 <  2:1274313/148‑1 (MQ=255)
               tCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGt                                                                                                                                 >  2:2316740/1‑148 (MQ=255)
               tCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGt                                                                                                                                 <  1:560418/148‑1 (MQ=255)
               tCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCGGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGt                                                                                                                                 >  1:2564712/1‑148 (MQ=255)
                cAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTa                                                                                                                                <  2:1830715/148‑1 (MQ=255)
                   tAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATg                                                                                                                             >  1:3172579/1‑148 (MQ=255)
                   tAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATg                                                                                                                             >  2:2099780/1‑148 (MQ=255)
                   tAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATg                                                                                                                             >  1:2135128/1‑148 (MQ=255)
                    aaaaTAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGt                                                                                                                            >  1:3411036/1‑148 (MQ=255)
                    aaaaTAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGt                                                                                                                            <  1:2503791/148‑1 (MQ=255)
                       aTAAAGCCTATATACACTGCCAGCATAATACTCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAgg                                                                                                                         <  1:3163130/148‑1 (MQ=255)
                       aTAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAgg                                                                                                                         >  1:3232524/1‑148 (MQ=255)
                        tAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAggg                                                                                                                        >  1:2979731/1‑148 (MQ=255)
                          aaGCCGATATAAACTGCCAGAATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGAt                                                                                                                      <  1:1666791/148‑1 (MQ=255)
                           aGCCGATATAAACTGCCAGCATAATACTCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGAtt                                                                                                                     <  2:791824/148‑1 (MQ=255)
                            gCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATACATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTg                                                                                                                    >  2:1524372/1‑148 (MQ=255)
                            gCCGATATAAACTGCAAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTg                                                                                                                    <  1:1630434/148‑1 (MQ=255)
                               tatatAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGttt                                                                                                                 <  1:984992/147‑1 (MQ=255)
                                atatAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGtttt                                                                                                                >  2:1099579/1‑148 (MQ=255)
                                 tataAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTa                                                                                                               <  1:358056/148‑1 (MQ=255)
                                  ataAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTaa                                                                                                              >  2:604419/1‑148 (MQ=255)
                                  ataAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTaa                                                                                                              <  1:391700/148‑1 (MQ=255)
                                    aaaCTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAAtt                                                                                                            <  1:1816653/148‑1 (MQ=255)
                                    aaaCTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAAtt                                                                                                            >  2:2935918/1‑148 (MQ=255)
                                        tGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCg                                                                                                        >  1:1575718/1‑148 (MQ=255)
                                         gCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCGGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGc                                                                                                       >  1:1574003/1‑148 (MQ=255)
                                           cggCAGAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTCATGGAGGGATTGTTTTAATTCCCGCTc                                                                                                     >  2:178817/3‑148 (MQ=255)
                                            aGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTcc                                                                                                    <  1:263669/148‑1 (MQ=255)
                                            aGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTcc                                                                                                    <  2:1223091/148‑1 (MQ=255)
                                            aGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTcc                                                                                                    <  1:1427275/148‑1 (MQ=255)
                                             gCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTccc                                                                                                   <  1:1190828/148‑1 (MQ=255)
                                              cATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCt                                                                                                  <  1:2581383/148‑1 (MQ=255)
                                               ataataATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCAGTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCtt                                                                                                 <  1:1928475/148‑1 (MQ=255)
                                                  ataatCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATg                                                                                              >  1:2648697/1‑148 (MQ=255)
                                                   taatCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATgg                                                                                             >  1:1008495/1‑148 (MQ=255)
                                                   taatCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATgg                                                                                             >  2:3358280/1‑148 (MQ=255)
                                                    aatCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCAGAAATGCGCATTTTCTTCTATCCGCTGATAAATAGTGCCATTCATAACATATTCTCCAGAGGTAATGTAGGGATTTTTTTAATTCCCGCTCCCTTATggg                                                                                            >  1:3290444/1‑148 (MQ=255)
                                                     atCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGga                                                                                           <  2:2652335/148‑1 (MQ=255)
                                                      tCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTTCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGgag                                                                                          >  1:2286066/1‑148 (MQ=255)
                                                      tCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGgag                                                                                          >  2:3277330/1‑148 (MQ=255)
                                                      tCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGgag                                                                                          >  1:292682/1‑148 (MQ=255)
                                                         aCAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAg                                                                                       >  2:1452568/1‑148 (MQ=255)
                                                          cAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGATATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAgg                                                                                      <  1:3410667/148‑1 (MQ=255)
                                                          cAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTATTTCCCGCTCCCTTATGGGAGTAgg                                                                                      >  2:1501538/1‑148 (MQ=255)
                                                          cAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAgg                                                                                      >  2:804154/1‑148 (MQ=255)
                                                          cAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAgg                                                                                      <  1:1299974/148‑1 (MQ=255)
                                                            ggATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGtt                                                                                    <  2:2738642/148‑1 (MQ=255)
                                                              agggtggCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCAGTGTCTTCTATCCGCGGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTaa                                                                                  <  1:359446/146‑1 (MQ=255)
                                                              aTGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGGAGGGATTGTTTTAATTCCCGCTCCCGTATGGGAGAAGGTTaa                                                                                  >  2:2601860/1‑148 (MQ=255)
                                                                 gtggCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGc                                                                               >  1:51948/1‑148 (MQ=255)
                                                                            ttGCCGTTTTTAGACTAACTCCCAGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCGTATGGGAGAAGGTTAACGCTCGGGTAAccc                                                                    >  1:125435/1‑148 (MQ=255)
                                                                             tGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCt                                                                   <  2:1163714/148‑1 (MQ=255)
                                                                               ccGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTg                                                                 <  1:605717/148‑1 (MQ=255)
                                                                                 gTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGcc                                                               >  2:2965678/1‑148 (MQ=255)
                                                                                 gTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGcc                                                               >  2:2516073/1‑148 (MQ=255)
                                                                                  tttttCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCg                                                              >  1:2342614/1‑148 (MQ=255)
                                                                                             aCTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCg                                                   >  2:1260404/1‑148 (MQ=255)
                                                                                              cTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCgg                                                  >  1:2512041/1‑148 (MQ=255)
                                                                                               tCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGa                                                 >  2:1922261/1‑148 (MQ=255)
                                                                                                     aaaTGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGc                                           >  1:2412141/1‑148 (MQ=255)
                                                                                                     aaaTGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGc                                           <  1:63383/148‑1 (MQ=255)
                                                                                                     aaaTGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTGATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGc                                           >  1:2093832/1‑148 (MQ=255)
                                                                                                       aTGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGt                                         >  2:2635482/1‑148 (MQ=255)
                                                                                                        tGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGtt                                        >  2:2958434/1‑148 (MQ=255)
                                                                                                         gcgcATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGttt                                       >  2:1672207/1‑148 (MQ=255)
                                                                                                         gcgcATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGttt                                       <  2:101756/148‑1 (MQ=255)
                                                                                                             aTTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTAcgc                                   >  2:2329422/1‑148 (MQ=255)
                                                                                                               tGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTAcgccg                                 >  1:2982431/1‑148 (MQ=255)
                                                                                                                 tcttctTTCCGCTGTTATATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCa                               <  2:904063/148‑1 (MQ=255)
                                                                                                                 tcttctATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCa                               >  2:730526/1‑148 (MQ=255)
                                                                                                                    tctATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCTAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTGATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATcc                            >  2:2171966/1‑148 (MQ=255)
                                                                                                                       aTCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGc                         >  2:2718879/1‑148 (MQ=255)
                                                                                                                        tCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGca                        >  2:2476007/1‑148 (MQ=255)
                                                                                                                        tCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGca                        >  2:3398184/1‑148 (MQ=255)
                                                                                                                        tCCGCCGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGca                        >  2:2893648/1‑148 (MQ=255)
                                                                                                                         ccGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGcaa                       <  1:2370506/148‑1 (MQ=255)
                                                                                                                         ccGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGcaa                       >  1:2024848/1‑148 (MQ=255)
                                                                                                                         ccGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGcaa                       <  1:3500909/148‑1 (MQ=255)
                                                                                                                            cTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGcaatca                    <  1:2327443/148‑1 (MQ=255)
                                                                                                                            cTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGcaatca                    >  2:1354333/1‑148 (MQ=255)
                                                                                                                               aTAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATg                 >  2:514882/1‑148 (MQ=255)
                                                                                                                                tAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAAGTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGc                <  1:2277357/148‑1 (MQ=255)
                                                                                                                                   aTAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTg             <  2:1574003/148‑1 (MQ=255)
                                                                                                                                    tAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGa            <  2:846087/148‑1 (MQ=255)
                                                                                                                                      gTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATg          >  2:1345980/1‑148 (MQ=255)
                                                                                                                                      gTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATg          >  2:2595558/1‑148 (MQ=255)
                                                                                                                                       tGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGc         >  1:844086/1‑148 (MQ=255)
                                                                                                                                           aTTCATAACAGATTCTCCAGAGGTAATGTAGGGATTTTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACg     <  1:962141/148‑1 (MQ=255)
                                                                                                                                            ttCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGc    >  2:3183696/1‑148 (MQ=255)
                                                                                                                                             tcataaCAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCt   <  1:1103713/148‑1 (MQ=255)
                                                                                                                                              cataaCAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTg  <  1:3089799/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTG  >  CP009273/4274997‑4275286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑