Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,275,862 C→A 94.8% D480Y (GAT→TAT)  acs ← acetyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,275,8620CA94.8% 569.3 / 21.1 194D480Y (GAT→TAT) acsacetyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base C (6/4);  new base A (106/78);  total (112/82)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

Only 100 of 194 total aligned reads displayed.

ACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAG  >  CP009273/4275722‑4276006
                                                                                                                                            |                                                                                                                                                
aCGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAAc                                                                                                                                           <  2:1505686/148‑1 (MQ=255)
  ggTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAg                                                                                                                                         <  1:2660386/148‑1 (MQ=255)
    tGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCg                                                                                                                                       <  1:3154991/148‑1 (MQ=255)
      aCCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTa                                                                                                                                     <  1:636478/148‑1 (MQ=255)
      aCCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTa                                                                                                                                     >  1:1742013/1‑148 (MQ=255)
           agaCGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACgcgc                                                                                                                                <  1:540298/148‑1 (MQ=255)
               gTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGa                                                                                                                            >  2:3543204/1‑148 (MQ=255)
                ttCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGAc                                                                                                                           >  2:913895/1‑148 (MQ=255)
                 tCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGAcc                                                                                                                          <  1:1495940/148‑1 (MQ=255)
                 tCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGAcc                                                                                                                          >  2:620705/1‑148 (MQ=255)
                    gCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCgg                                                                                                                       >  1:1289830/1‑148 (MQ=255)
                     cACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGc                                                                                                                      >  2:2937172/1‑148 (MQ=255)
                          cgtcCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGa                                                                                                                 >  1:510393/1‑148 (MQ=255)
                          cgtcCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGa                                                                                                                 <  2:2391748/148‑1 (MQ=255)
                           gtcCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAg                                                                                                                <  2:3028055/148‑1 (MQ=255)
                            tcCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGt                                                                                                               >  1:2278740/1‑148 (MQ=255)
                            tcCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGt                                                                                                               <  1:2221647/148‑1 (MQ=255)
                              cacaCGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCg                                                                                                             <  1:2968617/148‑1 (MQ=255)
                                    cccGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGAtt                                                                                                       >  2:3318989/1‑148 (MQ=255)
                                     ccGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTa                                                                                                      >  2:1892427/1‑148 (MQ=255)
                                         ttATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAg                                                                                                  >  2:3403516/1‑148 (MQ=255)
                                         ttATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAg                                                                                                  >  2:1640508/1‑148 (MQ=255)
                                         ttATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAg                                                                                                  >  1:479218/1‑148 (MQ=255)
                                          tATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAgg                                                                                                 <  1:3465545/148‑1 (MQ=255)
                                           aTCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGc                                                                                                <  2:1988805/148‑1 (MQ=255)
                                               aGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTAcc                                                                                            <  1:3051615/148‑1 (MQ=255)
                                               aGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTAcc                                                                                            >  2:2807682/1‑148 (MQ=255)
                                               aGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTAcc                                                                                            >  1:2462375/1‑148 (MQ=255)
                                               aGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTAcc                                                                                            >  1:3507008/1‑148 (MQ=255)
                                                 tAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCtt                                                                                          >  1:3501848/1‑148 (MQ=255)
                                                 tAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCtt                                                                                          <  1:2808816/148‑1 (MQ=255)
                                                 tAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCtt                                                                                          >  1:3221023/1‑148 (MQ=255)
                                                  aaTAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAACTACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGGTCGTGATAGCCCAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCGTc                                                                                         >  1:2479854/1‑148 (MQ=255)
                                                    tAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCgg                                                                                       >  1:1561148/1‑148 (MQ=255)
                                                    tAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCgg                                                                                       >  1:219283/1‑148 (MQ=255)
                                                    tAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCgg                                                                                       <  2:1516793/148‑1 (MQ=255)
                                                      gCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTg                                                                                     >  1:2188675/1‑148 (MQ=255)
                                                       ccATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTgg                                                                                    >  1:2676955/1‑148 (MQ=255)
                                                        cATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGc                                                                                   <  2:1876691/148‑1 (MQ=255)
                                                          tCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGccc                                                                                 >  1:2819131/1‑148 (MQ=255)
                                                          tCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGccc                                                                                 >  1:2286525/1‑148 (MQ=255)
                                                           cTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGcccc                                                                                >  1:2936147/1‑148 (MQ=255)
                                                               aTCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTcc                                                                            >  1:1786632/1‑148 (MQ=255)
                                                                     aCGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCggg                                                                      <  1:2162668/148‑1 (MQ=255)
                                                                     aCGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCggg                                                                      <  1:2092117/148‑1 (MQ=255)
                                                                     aCGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCggg                                                                      <  1:2163653/148‑1 (MQ=255)
                                                                      cgcgcgCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGt                                                                     <  1:3116333/148‑1 (MQ=255)
                                                                          cgcCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGACCCCCTCCAGCGGGTTAcc                                                                 <  2:1553545/148‑1 (MQ=255)
                                                                              gTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCg                                                             <  1:473163/148‑1 (MQ=255)
                                                                               tCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGt                                                            <  2:2507406/148‑1 (MQ=255)
                                                                               tCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGt                                                            <  2:3018238/148‑1 (MQ=255)
                                                                               tCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGt                                                            <  1:756295/148‑1 (MQ=255)
                                                                                cgccgcTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGtt                                                           <  1:3235616/148‑1 (MQ=255)
                                                                                 gccgcTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTa                                                          <  1:2955698/148‑1 (MQ=255)
                                                                                 gccgcTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTa                                                          <  1:1432818/148‑1 (MQ=255)
                                                                                 gccgcTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTa                                                          <  2:510141/148‑1 (MQ=255)
                                                                                  ccgcTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTAt                                                         >  1:3549345/1‑148 (MQ=255)
                                                                                     cTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGa                                                      >  2:1733038/1‑148 (MQ=255)
                                                                                     cTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGa                                                      >  2:800319/1‑148 (MQ=255)
                                                                                        aaaTACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCa                                                   <  2:2944476/148‑1 (MQ=255)
                                                                                        aaaTACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATAGACCa                                                   >  1:2407484/1‑148 (MQ=255)
                                                                                         aaTACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAg                                                  <  2:1925350/148‑1 (MQ=255)
                                                                                          aTACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAgc                                                 >  1:1958564/1‑148 (MQ=255)
                                                                                              atatTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCg                                             >  1:3383597/1‑148 (MQ=255)
                                                                                              atatTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCg                                             >  1:1386943/1‑148 (MQ=255)
                                                                                                atTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGt                                           <  2:531220/148‑1 (MQ=255)
                                                                                                 tttttGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGtt                                          <  1:1029968/148‑1 (MQ=255)
                                                                                                  ttttGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTg                                         >  2:3481296/1‑148 (MQ=255)
                                                                                                    ttGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCa                                       >  1:3273179/1‑148 (MQ=255)
                                                                                                       aaGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGc                                    <  1:1347477/148‑1 (MQ=255)
                                                                                                       aaGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGc                                    >  2:2755918/1‑148 (MQ=255)
                                                                                                        aGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGcc                                   >  2:3556144/1‑148 (MQ=255)
                                                                                                         ggTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCg                                  >  2:3014247/1‑148 (MQ=255)
                                                                                                             gagaAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCgaaga                              >  1:2372094/1‑148 (MQ=255)
                                                                                                             gagaAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCgaaga                              <  1:1192827/148‑1 (MQ=255)
                                                                                                                aaGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACg                           <  2:2455980/148‑1 (MQ=255)
                                                                                                                aaGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACg                           >  2:2234774/1‑148 (MQ=255)
                                                                                                                aaGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACg                           <  2:2188739/148‑1 (MQ=255)
                                                                                                                aaGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACg                           >  2:2182371/1‑148 (MQ=255)
                                                                                                                aaGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGATATCGACCAGCGCCGGTTGCACGCCgaacaccg                           >  2:445345/1‑143 (MQ=255)
                                                                                                                 aGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACgg                          <  2:588825/148‑1 (MQ=255)
                                                                                                                  gTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGa                         >  2:2700552/1‑148 (MQ=255)
                                                                                                                  gTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGa                         >  2:2880859/1‑148 (MQ=255)
                                                                                                                    aGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACg                       <  2:3053583/148‑1 (MQ=255)
                                                                                                                     ggTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACgt                      >  1:3072996/1‑148 (MQ=255)
                                                                                                                      gTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACgtg                     >  1:378131/1‑148 (MQ=255)
                                                                                                                       tCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACgtgt                    >  1:264163/1‑148 (MQ=255)
                                                                                                                       tCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACgtgt                    <  2:3501809/148‑1 (MQ=255)
                                                                                                                           ttCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGcc                >  2:1965884/1‑148 (MQ=255)
                                                                                                                           ttCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGcc                >  2:1353713/1‑148 (MQ=255)
                                                                                                                                aaCGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAAcc           <  1:952796/148‑1 (MQ=255)
                                                                                                                                aaCGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAAcc           >  2:861178/1‑148 (MQ=255)
                                                                                                                                 aCGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCg          >  2:1082895/1‑148 (MQ=255)
                                                                                                                                  cGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCgg         >  1:2537168/1‑148 (MQ=255)
                                                                                                                                       gTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTc    <  1:3498645/148‑1 (MQ=255)
                                                                                                                                        tGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCa   >  1:979855/1‑148 (MQ=255)
                                                                                                                                        tGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCa   >  1:1847486/1‑148 (MQ=255)
                                                                                                                                        tGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCa   >  1:1799866/1‑148 (MQ=255)
                                                                                                                                        tGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCa   >  1:1096554/1‑148 (MQ=255)
                                                                                                                                         gATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAg  >  1:1016197/1‑148 (MQ=255)
                                                                                                                                            |                                                                                                                                                
ACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAG  >  CP009273/4275722‑4276006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑