Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 337,720 G→T 89.6% E295D (GAG→GAT)  yahJ → putative metallo‑dependent hydrolase domain deaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273337,7200GT89.6% 527.8 / 51.1 211E295D (GAG→GAT) yahJputative metallo‑dependent hydrolase domain deaminase
Reads supporting (aligned to +/- strand):  ref base G (11/12);  new base T (107/81);  total (118/93)
Fisher's exact test for biased strand distribution p-value = 5.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 215 total aligned reads displayed.

CCGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGAGAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGATTGGCACGCTG  >  CP009273/337574‑337864
                                                                                                                                                  |                                                                                                                                                
cctaCCATTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCTATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAgaga                                                                                                                                                 <  1:3421905/145‑1 (MQ=255)
 cGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATaa                                                                                                                                                >  2:3380179/1‑148 (MQ=255)
     cAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACg                                                                                                                                            >  2:581864/1‑148 (MQ=255)
         gtTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCcac                                                                                                                                        >  2:1850857/1‑148 (MQ=255)
         gtTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCcac                                                                                                                                        >  2:246841/1‑148 (MQ=255)
           tGATGGCGCGATGGAAAAATCCCTCGACACCATGTGCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAg                                                                                                                                      <  2:2933602/148‑1 (MQ=255)
              tGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTg                                                                                                                                   >  2:496535/1‑148 (MQ=255)
               ggCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGa                                                                                                                                  >  1:173435/1‑148 (MQ=255)
                gcgcGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGaa                                                                                                                                 >  2:2697591/1‑148 (MQ=255)
                    gATGTAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGc                                                                                                                             <  2:1557522/148‑1 (MQ=255)
                      tGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCaa                                                                                                                           >  1:2911137/1‑148 (MQ=255)
                         aaaaaTCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCt                                                                                                                        >  2:437897/1‑148 (MQ=255)
                         aaaaaTCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCt                                                                                                                        >  1:3021421/1‑148 (MQ=255)
                          aaaaTCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTg                                                                                                                       <  2:228312/148‑1 (MQ=255)
                           aaaTCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGa                                                                                                                      <  2:2367129/148‑1 (MQ=255)
                            aaTCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGAc                                                                                                                     >  2:531771/1‑148 (MQ=255)
                             aTCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGAGAAAACGCCACAGCTGAAGGGCAAGCTGAcc                                                                                                                    >  1:1299397/1‑148 (MQ=255)
                                 cTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCa                                                                                                                <  2:2544490/148‑1 (MQ=255)
                                  tCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAg                                                                                                               >  2:3407051/1‑148 (MQ=255)
                                  tCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAg                                                                                                               >  1:301472/1‑148 (MQ=255)
                                   cGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGAGAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGt                                                                                                              >  1:1485012/1‑148 (MQ=255)
                                      cacCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCAc                                                                                                           >  2:3170812/1‑148 (MQ=255)
                                        ccATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGc                                                                                                         >  2:2122531/1‑148 (MQ=255)
                                        ccATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGc                                                                                                         >  2:3391928/1‑148 (MQ=255)
                                         cATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGcc                                                                                                        <  2:143063/148‑1 (MQ=255)
                                         cATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGcc                                                                                                        >  1:1127606/1‑148 (MQ=255)
                                          aTGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCt                                                                                                       <  2:1647591/148‑1 (MQ=255)
                                          aTGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCt                                                                                                       <  1:3457272/148‑1 (MQ=255)
                                          aTGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCt                                                                                                       >  1:2153753/1‑148 (MQ=255)
                                          aTGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGAGAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCt                                                                                                       <  2:1485012/148‑1 (MQ=255)
                                              tCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTgc                                                                                                   >  1:1901759/1‑148 (MQ=255)
                                                ctgATTGCGCTTGACTACGACAATGGCTTCGATTTTCTCATGCTCGAAACCACTCCGGCAGGCGTGTCAGCAATCAATTATTTTTTTGAAACGGTAGATAAAACTCCACAGCTGAAGGTAATGCTTTGCTTCAGTGACGCCTTTgcgg                                                                                                 <  2:564715/146‑2 (MQ=255)
                                                 agagggcgcTGGACTACGACAACGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCt                                                                                                <  1:1208986/143‑1 (MQ=255)
                                                  gATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTg                                                                                               <  2:994240/148‑1 (MQ=255)
                                                   aTTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTgg                                                                                              <  2:2154619/148‑1 (MQ=255)
                                                     tGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCa                                                                                            <  2:3113363/148‑1 (MQ=255)
                                                      gcgcTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCaa                                                                                           <  2:3390379/148‑1 (MQ=255)
                                                       cgcTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAAc                                                                                          >  1:1265281/1‑148 (MQ=255)
                                                       cgcTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAAc                                                                                          >  1:1797420/1‑148 (MQ=255)
                                                         cTGTACTACTACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTTTATGGTTGAAACGGTAGAGAAAACGCCACAGCTGAAGGGAAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGc                                                                                        <  1:153436/148‑1 (MQ=255)
                                                         cTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGt                                                                                                              >  1:34043/1‑126 (MQ=255)
                                                         cTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGt                                                                                                              <  2:34043/126‑1 (MQ=255)
                                                         cTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGc                                                                                        <  1:1003536/148‑1 (MQ=255)
                                                         cTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGc                                                                                        <  1:2780692/148‑1 (MQ=255)
                                                          tGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCt                                                                                       <  2:148725/148‑1 (MQ=255)
                                                          tGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCt                                                                                       <  1:2694843/148‑1 (MQ=255)
                                                             aCTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCaa                                                                                    <  2:1343305/148‑1 (MQ=255)
                                                                 cGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAg                                                                                >  2:1123243/1‑148 (MQ=255)
                                                                   aCAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCa                                                                              >  1:957866/1‑148 (MQ=255)
                                                                   aCAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCa                                                                              >  1:3002203/1‑148 (MQ=255)
                                                                    cAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCaa                                                                             >  2:126571/1‑148 (MQ=255)
                                                                      aaGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACa                                                                           >  1:3434113/1‑148 (MQ=255)
                                                                       aGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAg                                                                          >  2:2797246/1‑148 (MQ=255)
                                                                        ggCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAgg                                                                         >  2:2069641/1‑148 (MQ=255)
                                                                         gCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGt                                                                        >  1:1589361/1‑148 (MQ=255)
                                                                            tCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGCGCAACAGGTAGa                                                                     >  2:2170252/1‑148 (MQ=255)
                                                                            tCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGa                                                                     >  2:2170223/1‑148 (MQ=255)
                                                                             cGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGAt                                                                    >  1:2560311/1‑148 (MQ=255)
                                                                             cGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGAt                                                                    >  1:525095/1‑148 (MQ=255)
                                                                                tatTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGaa                                                                 <  2:254165/148‑1 (MQ=255)
                                                                                tatTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGaa                                                                 >  1:1248977/1‑148 (MQ=255)
                                                                                 atTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAAc                                                                >  2:702126/1‑148 (MQ=255)
                                                                                 atTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAAc                                                                >  1:1583362/1‑148 (MQ=255)
                                                                                  ttCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACt                                                               <  1:2860903/148‑1 (MQ=255)
                                                                                  ttCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACt                                                               >  2:1396653/1‑148 (MQ=255)
                                                                                  ttCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACt                                                               <  1:161506/148‑1 (MQ=255)
                                                                                   tCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGAGAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTg                                                              <  1:3132432/148‑1 (MQ=255)
                                                                                    cACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGAGAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTgg                                                             <  2:69281/148‑1 (MQ=255)
                                                                                     aCCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGAGAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGc                                                            >  2:779383/1‑148 (MQ=255)
                                                                                            cGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCgg                                                     >  1:27876/1‑148 (MQ=255)
                                                                                              aaaCCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGAt                                                   >  1:525553/1‑148 (MQ=255)
                                                                                              aaaCCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGAt                                                   >  1:525755/1‑148 (MQ=255)
                                                                                               aaCCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGAtg                                                  <  1:3119341/148‑1 (MQ=255)
                                                                                                    cTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCa                                                                                     <  2:837461/108‑1 (MQ=255)
                                                                                                     tCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGgtggtg                                            >  1:595568/1‑148 (MQ=255)
                                                                                                      ccGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGc                                           >  2:1160382/1‑148 (MQ=255)
                                                                                                           aGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGcaacaa                                      <  1:873472/148‑1 (MQ=255)
                                                                                                            ggagtgtCAGCAATCAATTATTTGGTTGAAACGGTAGATAAATCGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACaaa                                     <  2:1391547/145‑1 (MQ=255)
                                                                                                            ggCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACaaa                                     >  2:1539039/1‑148 (MQ=255)
                                                                                                            ggCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACaaa                                     <  2:3375127/148‑1 (MQ=255)
                                                                                                             gCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAAt                                    >  1:457704/1‑148 (MQ=255)
                                                                                                             gCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAAt                                    >  1:2287215/1‑148 (MQ=255)
                                                                                                              cGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAAtt                                   <  1:1014571/148‑1 (MQ=255)
                                                                                                               gTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAAttt                                  <  1:3540171/148‑1 (MQ=255)
                                                                                                                 ggCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCt                                <  2:2831932/148‑1 (MQ=255)
                                                                                                                 ggCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCt                                <  2:364769/148‑1 (MQ=255)
                                                                                                                  gCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTa                               >  1:2656898/1‑148 (MQ=255)
                                                                                                                      ccATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGc                           <  1:1850857/148‑1 (MQ=255)
                                                                                                                           aaTTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGa                      <  2:2609737/148‑1 (MQ=255)
                                                                                                                            aTTATATGGTTGAAACGGTAGAGAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGAc                     >  2:915819/1‑148 (MQ=255)
                                                                                                                             ttATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCTAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACg                    >  1:1934698/1‑148 (MQ=255)
                                                                                                                             ttATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACg                    >  1:136454/1‑148 (MQ=255)
                                                                                                                              tataTGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACgg                   <  2:2911137/148‑1 (MQ=255)
                                                                                                                                 atGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGc                >  2:3293432/1‑148 (MQ=255)
                                                                                                                                    gTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGa             <  1:3170812/148‑1 (MQ=255)
                                                                                                                                    gTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGa             <  1:280594/148‑1 (MQ=255)
                                                                                                                                      tGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGAtt           >  2:2171262/1‑148 (MQ=255)
                                                                                                                                            ggTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTTCCGATTGGCACg     <  2:1385151/148‑1 (MQ=255)
                                                                                                                                               agatAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGATTGGCACGCTg  <  2:462615/148‑1 (MQ=255)
                                                                                                                                               agatAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGATTGGCACGCTg  >  1:845500/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
CCGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGAGAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGATTGGCACGCTG  >  CP009273/337574‑337864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑