Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,303,614 T→A 100% *150R (TGA→AGA)  rpiB → ribose 5‑phosphate isomerase B/allose 6‑phosphate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,303,6140TA98.9% 601.3 / ‑2.4 182*150R (TGA→AGA) rpiBribose 5‑phosphate isomerase B/allose 6‑phosphate isomerase
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base A (89/91);  total (90/92)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 187 total aligned reads displayed.

CACCAACGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATTGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTA  >  CP009273/4303469‑4303757
                                                                                                                                                 |                                                                                                                                               
cacCAACGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAga                                                                                                                                               >  1:338199/1‑148 (MQ=255)
cacCAACGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAga                                                                                                                                               >  1:338183/1‑148 (MQ=255)
 acCAACGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATagag                                                                                                                                              >  2:1261342/1‑148 (MQ=255)
 acCAACGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATagag                                                                                                                                              >  2:1577147/1‑148 (MQ=255)
 acCAACGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATagag                                                                                                                                              >  2:1577302/1‑148 (MQ=255)
   cAACGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGAt                                                                                                                                            <  1:895719/148‑1 (MQ=255)
   cAACGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGAt                                                                                                                                            <  1:1229381/148‑1 (MQ=255)
   cAACGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGAt                                                                                                                                            >  1:2060356/1‑148 (MQ=255)
     aCGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTc                                                                                                                                          <  1:3154378/148‑1 (MQ=255)
      cGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCa                                                                                                                                         <  1:2696149/148‑1 (MQ=255)
         gCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATTGAGATTCATcc                                                                                                                                      >  1:3441191/1‑148 (MQ=255)
         gCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATcc                                                                                                                                      <  2:1582665/148‑1 (MQ=255)
          cTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCa                                                                                                                                     <  1:1098788/148‑1 (MQ=255)
           tGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCac                                                                                                                                    <  2:210071/148‑1 (MQ=255)
            ggCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCact                                                                                                                                   >  2:3192175/1‑148 (MQ=255)
            ggCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCact                                                                                                                                   >  2:847084/1‑148 (MQ=255)
                tttGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACtt                                                                                                                               >  2:3349097/1‑148 (MQ=255)
                tttGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACtt                                                                                                                               >  1:1809727/1‑148 (MQ=255)
                tttGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACtt                                                                                                                               >  1:1808082/1‑148 (MQ=255)
                tttGGTTCACGAGTGGTTGGCCTCGAACTGACAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACtt                                                                                                                               >  1:805998/1‑148 (MQ=255)
                    gTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCAt                                                                                                                           >  2:1633214/1‑148 (MQ=255)
                     ttCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATg                                                                                                                          <  2:584585/148‑1 (MQ=255)
                       cACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGa                                                                                                                        >  2:3195200/1‑148 (MQ=255)
                       cACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGa                                                                                                                        <  2:2305/148‑1 (MQ=255)
                         cGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATg                                                                                                                      <  2:3145901/148‑1 (MQ=255)
                         cGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATg                                                                                                                      <  2:818254/148‑1 (MQ=255)
                              ggTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACAGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTaa                                                                                                                 >  1:2879608/1‑148 (MQ=255)
                                ttGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATg                                                                                                               <  2:1377544/148‑1 (MQ=255)
                                   gCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGAtt                                                                                                            >  2:2854216/1‑148 (MQ=255)
                                     cTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGCACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTaa                                                                                                          <  1:1890689/148‑1 (MQ=255)
                                         aaCTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAAtgtg                                                                                                      >  2:1259584/1‑148 (MQ=255)
                                         aaCTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAAtgtg                                                                                                      <  2:1003074/148‑1 (MQ=255)
                                         aaCTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAAtgtg                                                                                                      >  1:1002555/1‑148 (MQ=255)
                                          aCTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTgg                                                                                                     >  1:196483/1‑148 (MQ=255)
                                           cTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGa                                                                                                    >  1:628104/1‑148 (MQ=255)
                                           cTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGa                                                                                                    >  1:2248012/1‑148 (MQ=255)
                                               cAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATaga                                                                                                >  2:3077419/1‑148 (MQ=255)
                                               cAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATACACTACTTGCATGGATGAGTAATGATTAATGTGGATaga                                                                                                >  1:2853311/1‑148 (MQ=255)
                                                aaaaaTGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATTGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATagag                                                                                               <  2:2482148/148‑1 (MQ=255)
                                                 aaaaTGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGt                                                                                              <  1:3028753/148‑1 (MQ=255)
                                                  aaaTGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGtt                                                                                             <  2:2089371/148‑1 (MQ=255)
                                                  aaaTGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGtt                                                                                             <  2:2854531/148‑1 (MQ=255)
                                                   aaTGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGttt                                                                                            >  2:276560/1‑148 (MQ=255)
                                                   aaTGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGttt                                                                                            >  2:137070/1‑148 (MQ=255)
                                                    aTGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTc                                                                                           <  2:1153965/148‑1 (MQ=255)
                                                     tGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCt                                                                                          <  1:3194982/148‑1 (MQ=255)
                                                      taTTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCtt                                                                                         >  2:3230470/2‑148 (MQ=255)
                                                       aTTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTGGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCttt                                                                                        >  1:2805724/1‑148 (MQ=255)
                                                       aTTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCttt                                                                                        >  1:1030209/1‑148 (MQ=255)
                                                              aTGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGt                                                                                 <  2:635406/148‑1 (MQ=255)
                                                               tGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATTGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGtt                                                                                <  2:3441191/148‑1 (MQ=255)
                                                                gCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTg                                                                               >  1:857676/1‑148 (MQ=255)
                                                                      cTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAAt                                                                         <  1:472648/148‑1 (MQ=255)
                                                                              acaGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCt                                                                 >  2:1049461/1‑148 (MQ=255)
                                                                              acaGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCt                                                                 <  2:1764399/148‑1 (MQ=255)
                                                                               caGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCtt                                                                >  1:2923811/1‑148 (MQ=255)
                                                                               caGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGATAATGAAACGCtt                                                                <  1:2557660/148‑1 (MQ=255)
                                                                                aGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCttt                                                               <  2:1094892/148‑1 (MQ=255)
                                                                                aGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCttt                                                               <  2:2850207/148‑1 (MQ=255)
                                                                                    cGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGc                                                           >  1:3556442/1‑148 (MQ=255)
                                                                                      aaGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTa                                                         <  1:2387951/148‑1 (MQ=255)
                                                                                       aGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTAt                                                        >  1:2761771/1‑148 (MQ=255)
                                                                                       aGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTAt                                                        >  2:1890180/1‑148 (MQ=255)
                                                                                       aGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTAt                                                        >  1:1752005/1‑148 (MQ=255)
                                                                                        ggCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTAtt                                                       >  2:2624401/1‑148 (MQ=255)
                                                                                             tcgtcATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTACGGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTctttcttt                                                  >  2:2694738/1‑148 (MQ=255)
                                                                                             tcgtcATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCttt                                                  <  2:244035/148‑1 (MQ=255)
                                                                                               gtcATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTa                                                <  1:3320803/148‑1 (MQ=255)
                                                                                               gtcATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTa                                                <  1:2018331/148‑1 (MQ=255)
                                                                                                 catcaACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTAtt                                              >  2:3076791/1‑148 (MQ=255)
                                                                                                 catcaACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTAtt                                              <  2:1074145/148‑1 (MQ=255)
                                                                                                  atcaACACCGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTc                                             <  2:1556384/148‑1 (MQ=255)
                                                                                                  atcaACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTc                                             >  2:496140/1‑148 (MQ=255)
                                                                                                    caacaaCGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGAGGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCAc                                           >  2:2200758/1‑148 (MQ=255)
                                                                                                       caacGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCt                                        <  1:2854205/148‑1 (MQ=255)
                                                                                                        aacGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTg                                       >  1:3260684/1‑148 (MQ=255)
                                                                                                        aacGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGAGTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTg                                       <  1:2182614/148‑1 (MQ=255)
                                                                                                          cgcgTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTCTTTCTTTTATTAACCCTGCt                                     >  1:3300929/1‑148 (MQ=255)
                                                                                                          cgcgTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCt                                     <  2:2961371/148‑1 (MQ=255)
                                                                                                                   gCGATTACGGCAATAGAGCAGCGGAGAAATAGAGAGTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTc                            <  1:729516/148‑1 (MQ=255)
                                                                                                                       ttACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCAcc                        >  1:2861803/1‑148 (MQ=255)
                                                                                                                        tACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTTGCTAATGAAACGATTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCAcag                       >  1:2387459/1‑146 (MQ=255)
                                                                                                                               aTAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGc                >  2:2780806/1‑148 (MQ=255)
                                                                                                                               aTAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGc                <  2:196483/148‑1 (MQ=255)
                                                                                                                                tAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGcc               <  1:1789396/148‑1 (MQ=255)
                                                                                                                                  gagCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCa             <  2:1104016/148‑1 (MQ=255)
                                                                                                                                    gcagcGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAgg           <  1:2137803/148‑1 (MQ=255)
                                                                                                                                       gcGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCg        <  2:942304/148‑1 (MQ=255)
                                                                                                                                       gcGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCg        <  1:1579366/148‑1 (MQ=255)
                                                                                                                                        cGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGa       >  1:1885509/1‑148 (MQ=255)
                                                                                                                                        cGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGa       >  1:692786/1‑148 (MQ=255)
                                                                                                                                           agaAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGatat    >  2:3273420/1‑148 (MQ=255)
                                                                                                                                           agaAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGatat    >  1:3159067/1‑148 (MQ=255)
                                                                                                                                           agaAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGatat    >  1:3159555/1‑148 (MQ=255)
                                                                                                                                            gaAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGAtatt   <  2:2805724/148‑1 (MQ=255)
                                                                                                                                            gaAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGAtatt   <  2:367309/148‑1 (MQ=255)
                                                                                                                                             aaaTAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGAtatta  >  1:3263365/1‑148 (MQ=255)
                                                                                                                                             aaaTAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGAtatta  >  1:805451/1‑148 (MQ=255)
                                                                                                                                             aaaTAGAGATTCATCCACTACTTGCATGGATGAGAAATGATTAATGTGGATAGAGTTTCTTATAGAGGTTGGCTAATGAAACGCTATCCGCTATTTCTTTTATACACCCTGCTCACTCTGTCCACCGTTCCGGCCCAGGCCGatataa  >  2:277645/1‑146 (MQ=255)
                                                                                                                                             aaaTAGAGACTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGAtatta  >  1:3205153/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CACCAACGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATTGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTA  >  CP009273/4303469‑4303757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑