Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,318,517 A→T 100% T522T (ACA→ACT)  crfC → clamp‑binding sister replication fork colocalization protein, dynamin‑related

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,318,5170AT96.8% 587.7 / 5.2 186T522T (ACA→ACT) crfCclamp‑binding sister replication fork colocalization protein, dynamin‑related
Reads supporting (aligned to +/- strand):  ref base A (3/3);  new base T (69/111);  total (72/114)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 186 total aligned reads displayed.

CGAGATTAAACATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACACTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAGGGTTT  >  CP009273/4318373‑4318661
                                                                                                                                                |                                                                                                                                                
cgAGATTAAACATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTg                                                                                                                                               <  2:719532/148‑1 (MQ=255)
cgAGATTAAACATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTg                                                                                                                                               <  1:995614/148‑1 (MQ=255)
 gagaTTAAACATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGt                                                                                                                                              <  2:472468/148‑1 (MQ=255)
  agaTTAAACATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGtt                                                                                                                                             >  1:3024191/1‑148 (MQ=255)
   gaTTAAACATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTa                                                                                                                                            <  1:1363930/148‑1 (MQ=255)
   gaTTAAACATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTa                                                                                                                                            >  2:3206882/1‑148 (MQ=255)
     ttAAACATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACa                                                                                                                                          >  2:1593034/1‑148 (MQ=255)
      tAAACATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAg                                                                                                                                         <  2:1066336/148‑1 (MQ=255)
       aaaCATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGa                                                                                                                                        <  2:956379/148‑1 (MQ=255)
            tGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGgcg                                                                                                                                   <  1:886749/148‑1 (MQ=255)
            tGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGgcg                                                                                                                                   >  1:2097101/1‑148 (MQ=255)
             gAACTCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGgcgc                                                                                                                                  <  2:744993/148‑1 (MQ=255)
             gAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGgcgc                                                                                                                                  <  2:2529348/148‑1 (MQ=255)
             gAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGgcgc                                                                                                                                  <  2:1306711/148‑1 (MQ=255)
                 tCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAATCGAGATTCGTACCCGCTGTGAGACTCTGTTAAATACGGCGCAGaa                                                                                                                              >  1:1668958/1‑148 (MQ=255)
                 tCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGaa                                                                                                                              >  1:1668839/1‑148 (MQ=255)
                 tCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGaa                                                                                                                              >  1:774711/1‑148 (MQ=255)
                 tCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGaa                                                                                                                              >  1:739070/1‑148 (MQ=255)
                 tCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGaa                                                                                                                              <  2:2008397/148‑1 (MQ=255)
                   gAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAaca                                                                                                                            <  2:3126383/148‑1 (MQ=255)
                     gCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACAcc                                                                                                                          >  2:867040/1‑148 (MQ=255)
                       tGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCAt                                                                                                                        <  2:3026821/148‑1 (MQ=255)
                         gCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCa                                                                                                                      <  1:3299572/148‑1 (MQ=255)
                              gACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTcgc                                                                                                                 >  2:2579171/1‑148 (MQ=255)
                              gACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTcgc                                                                                                                 <  2:3206129/148‑1 (MQ=255)
                                ccTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGa                                                                                                               <  1:645524/148‑1 (MQ=255)
                                 cTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGAc                                                                                                              >  2:1164845/1‑148 (MQ=255)
                                  tCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACt                                                                                                             <  2:635969/148‑1 (MQ=255)
                                     gCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTa                                                                                                          <  2:1521385/148‑1 (MQ=255)
                                       cAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACg                                                                                                        <  1:2334849/148‑1 (MQ=255)
                                        aaCCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGc                                                                                                       >  1:1995589/1‑148 (MQ=255)
                                         aCCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCt                                                                                                      >  1:1547265/1‑148 (MQ=255)
                                          ccACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTg                                                                                                     >  1:2083186/1‑148 (MQ=255)
                                          ccACTGTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTg                                                                                                     <  2:2825374/148‑1 (MQ=255)
                                             cTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGt                                                                                                  <  1:3103096/148‑1 (MQ=255)
                                               ttCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGttt                                                                                                <  2:3534620/148‑1 (MQ=255)
                                               ttCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGttt                                                                                                >  1:244992/1‑148 (MQ=255)
                                                 cTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTg                                                                                              <  2:1385343/148‑1 (MQ=255)
                                                 cTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTg                                                                                              <  1:1491690/148‑1 (MQ=255)
                                                  tGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGc                                                                                             <  2:2206434/148‑1 (MQ=255)
                                                      tCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAg                                                                                         <  2:798229/148‑1 (MQ=255)
                                                       cAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGc                                                                                        <  1:3535021/148‑1 (MQ=255)
                                                       cAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGc                                                                                        <  1:3535009/148‑1 (MQ=255)
                                                       cAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGc                                                                                        <  1:1011487/148‑1 (MQ=255)
                                                        aaCAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCAGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCt                                                                                       >  1:3557740/1‑148 (MQ=255)
                                                          cAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTg                                                                                     <  1:2522585/148‑1 (MQ=255)
                                                          cAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTg                                                                                     >  2:1390056/1‑148 (MQ=255)
                                                            gCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGaa                                                                                   >  2:1247919/1‑148 (MQ=255)
                                                              aaGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATc                                                                                 <  2:1796802/148‑1 (MQ=255)
                                                               aGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATcc                                                                                >  2:3157354/1‑148 (MQ=255)
                                                               aGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATcc                                                                                >  1:3461392/1‑148 (MQ=255)
                                                                gATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCa                                                                               >  1:1405/1‑148 (MQ=255)
                                                                  tGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCAcc                                                                             <  2:3003463/148‑1 (MQ=255)
                                                                   gcgcTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCAccc                                                                            <  1:2574967/148‑1 (MQ=255)
                                                                     gcTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCtt                                                                          <  1:2806354/148‑1 (MQ=255)
                                                                      cTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACACTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCttt                                                                         <  2:2371979/148‑1 (MQ=255)
                                                                       tGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTg                                                                        <  2:1875393/148‑1 (MQ=255)
                                                                         aaGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGcc                                                                      >  1:2920691/1‑148 (MQ=255)
                                                                             tGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGgt                                                                  <  2:2755795/148‑1 (MQ=255)
                                                                                     gccgccTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCg                                                          <  2:2545667/148‑1 (MQ=255)
                                                                                      ccgccTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGa                                                         >  2:2220323/1‑148 (MQ=255)
                                                                                      ccgccTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGa                                                         >  2:430731/1‑148 (MQ=255)
                                                                                      ccgccTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGa                                                         <  1:2773981/148‑1 (MQ=255)
                                                                                        gccTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATg                                                       <  2:1995589/148‑1 (MQ=255)
                                                                                         ccTTGTTTCAGTATGTTTCTGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGt                                                      <  2:3319532/148‑1 (MQ=255)
                                                                                          cTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGtt                                                     <  2:930546/148‑1 (MQ=255)
                                                                                             gTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTAtt                                                  <  2:2865080/148‑1 (MQ=255)
                                                                                               ttCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCg                                                >  2:1624665/1‑148 (MQ=255)
                                                                                                   ggATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGcccc                                            >  1:2114305/1‑148 (MQ=255)
                                                                                                    gatgatTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGcccaa                                           <  2:760171/148‑3 (MQ=255)
                                                                                                    gatgatTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTAGCCCCa                                           >  1:1261397/1‑148 (MQ=255)
                                                                                                     atgttTCGGAGCCGTTTAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACTGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGTTGTTAACCGATGTTATTCGCCCCAt                                          <  2:1353008/148‑1 (MQ=255)
                                                                                                     atgatTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCAt                                          <  2:2168749/148‑1 (MQ=255)
                                                                                                     atgagTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCAt                                          <  2:2273964/148‑1 (MQ=255)
                                                                                                      tgatTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCAtt                                         >  1:3441872/1‑148 (MQ=255)
                                                                                                        atTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGa                                       >  2:3266789/1‑148 (MQ=255)
                                                                                                         ttCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACACTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAg                                      >  2:2240208/1‑148 (MQ=255)
                                                                                                           cGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGca                                    <  1:1905056/148‑1 (MQ=255)
                                                                                                            ggAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGcaa                                   <  1:104732/148‑1 (MQ=255)
                                                                                                            ggAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGcaa                                   <  1:317406/148‑1 (MQ=255)
                                                                                                              aGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGcaaca                                 <  2:3338716/148‑1 (MQ=255)
                                                                                                              aGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACACTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGcaaca                                 >  2:2770814/1‑148 (MQ=255)
                                                                                                                    tAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCaa                           >  2:2937834/1‑148 (MQ=255)
                                                                                                                     aaGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCCACAAGTCaaa                          <  1:3206882/148‑1 (MQ=255)
                                                                                                                          agaTTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGa                     >  1:2453848/1‑148 (MQ=255)
                                                                                                                          agaTTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGa                     <  2:1046210/148‑1 (MQ=255)
                                                                                                                             ttCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAAtt                  >  1:2417542/1‑148 (MQ=255)
                                                                                                                              tCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTg                 <  2:602730/148‑1 (MQ=255)
                                                                                                                                  aCCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCg             >  1:1118145/1‑148 (MQ=255)
                                                                                                                                   cccGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGa            >  1:3501161/1‑148 (MQ=255)
                                                                                                                                   cccGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGa            <  1:2591072/148‑1 (MQ=255)
                                                                                                                                   cccGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGa            >  2:2680951/1‑148 (MQ=255)
                                                                                                                                    ccGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAg           >  1:1055415/1‑148 (MQ=255)
                                                                                                                                     cGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGt          >  2:1180806/1‑148 (MQ=255)
                                                                                                                                      gCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTc         <  2:2635676/148‑1 (MQ=255)
                                                                                                                                         gtgAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAgg      >  2:3475526/1‑148 (MQ=255)
                                                                                                                                         gtgAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAgg      >  2:3475525/1‑148 (MQ=255)
                                                                                                                                         gtgAGACTCTGTTACAGACGGAGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAgg      >  1:3088555/1‑148 (MQ=255)
                                                                                                                                           gagaCTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAGGGt    <  2:1443927/148‑1 (MQ=255)
                                                                                                                                             gaCTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAGGGttt  <  2:840652/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
CGAGATTAAACATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACACTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAGGGTTT  >  CP009273/4318373‑4318661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑