Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,329,774 C→A 100% D189Y (GAC→TAC)  adiA ← arginine decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,329,7740CA97.0% 451.5 / 6.6 166D189Y (GAC→TAC) adiAarginine decarboxylase
Reads supporting (aligned to +/- strand):  ref base C (2/3);  new base A (84/73);  total (86/80)
Fisher's exact test for biased strand distribution p-value = 6.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.01e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 166 total aligned reads displayed.

GTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTCGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCTGAACAGTGG  >  CP009273/4329632‑4329918
                                                                                                                                              |                                                                                                                                                
gTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGc                                                                                                                                             <  1:2976852/148‑1 (MQ=255)
     gTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAAc                                                                                                                                        <  2:3040121/148‑1 (MQ=255)
        agagCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGa                                                                                                                                     >  2:2484288/1‑148 (MQ=255)
         gagCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTCGGTGCGGAACAGAt                                                                                                                                    >  1:535604/1‑148 (MQ=255)
         gagCCGGAAGAACCGACGACTACCGACCAGGAGCTATAGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGAGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGAATCCAAGATATGTGCGGAACAGAt                                                                                                                                    >  2:250647/1‑148 (MQ=255)
               gAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCAc                                                                                                                              >  1:2296294/1‑148 (MQ=255)
                aaGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCAcc                                                                                                                             >  1:612499/1‑148 (MQ=255)
                 attACCGACGACTACCGACCAGGAGAGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCAGGTAGGTGAGTAACAGATTTTCAcga                                                                                                                            >  2:2148668/3‑146 (MQ=255)
                 aGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGGTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCa                                                                                                                            >  2:3288224/1‑148 (MQ=255)
                      cgacgaCTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCAtagta                                                                                                                       <  2:2562696/148‑1 (MQ=255)
                       gacgacTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCAtagtag                                                                                                                      <  1:895762/148‑1 (MQ=255)
                        acgacTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATagtagt                                                                                                                     >  2:1781453/1‑148 (MQ=255)
                         cgacTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTc                                                                                                                    >  2:2837702/1‑148 (MQ=255)
                          gacTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCa                                                                                                                   <  2:50716/148‑1 (MQ=255)
                            cTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATg                                                                                                                 <  2:2564510/148‑1 (MQ=255)
                             tACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATgg                                                                                                                <  2:386090/148‑1 (MQ=255)
                             tACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATgg                                                                                                                <  1:197221/148‑1 (MQ=255)
                              aCCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGt                                                                                                               <  2:323435/148‑1 (MQ=255)
                              aCCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGt                                                                                                               <  2:795330/148‑1 (MQ=255)
                              aCCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGt                                                                                                               <  1:2551907/148‑1 (MQ=255)
                                  aCCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGaa                                                                                                           <  1:216417/148‑1 (MQ=255)
                                     aGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACg                                                                                                        <  2:3206075/148‑1 (MQ=255)
                                       gAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTc                                                                                                      <  1:78304/148‑1 (MQ=255)
                                         gCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCcg                                                                                                    >  2:2027048/1‑148 (MQ=255)
                                          cGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGACGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCcgg                                                                                                   >  2:3107195/1‑148 (MQ=255)
                                            aTCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCcggcg                                                                                                 >  2:3431103/1‑148 (MQ=255)
                                             tCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCcggcgg                                                                                                >  2:1957134/1‑148 (MQ=255)
                                             tCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCcggcgg                                                                                                >  2:2522892/1‑148 (MQ=255)
                                             tCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCcggcgg                                                                                                >  2:2522891/1‑148 (MQ=255)
                                              cGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGACCAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCggg                                                                                               >  2:1468027/1‑148 (MQ=255)
                                                 cACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCcggcgg                                                                                                >  2:1999693/1‑144 (MQ=255)
                                                   ccAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGttt                                                                                          >  2:2888627/1‑148 (MQ=255)
                                                   ccAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGttt                                                                                          >  2:3545221/1‑148 (MQ=255)
                                                     aaaTACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTg                                                                                        >  1:1371549/1‑148 (MQ=255)
                                                      aaTACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTgg                                                                                       <  1:2566536/148‑1 (MQ=255)
                                                      aaTACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTgg                                                                                       >  2:1901170/1‑148 (MQ=255)
                                                       aTACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGt                                                                                      <  2:2314276/148‑1 (MQ=255)
                                                         ccgcgGGCGGCATATTTTTCGCTTTCGCCAACTGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTaa                                                                                    <  2:2461344/147‑1 (MQ=255)
                                                              tGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCa                                                                               >  1:1368103/1‑148 (MQ=255)
                                                              tGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCa                                                                               >  1:1368129/1‑148 (MQ=255)
                                                              tGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCa                                                                               <  1:2025265/148‑1 (MQ=255)
                                                              tGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCa                                                                               >  2:1470081/1‑148 (MQ=255)
                                                              tGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCa                                                                               <  2:1201175/148‑1 (MQ=255)
                                                                  gCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAAcgc                                                                           <  2:2728200/148‑1 (MQ=255)
                                                                  gCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAAcgc                                                                           <  2:1345741/148‑1 (MQ=255)
                                                                     tatTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAAcgccgc                                                                        >  2:1296971/1‑148 (MQ=255)
                                                                     tatTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAAcgccgc                                                                        >  2:212446/1‑148 (MQ=255)
                                                                     tatTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAAcgccgc                                                                        >  1:1187893/1‑148 (MQ=255)
                                                                       tttttCGCTTTCGCCAAATGCCCCTTTTTGTTAAAGCAAACAACGGAGGGAAGTACGTTCGCTGCCCATGTAGGGGGGTAACATTTTTACACCTATGTAGTCTTGTTATAAACGTCCGGCGGTTTTTTTGTTAAAACCAACGCCGccc                                                                      <  1:1286931/148‑1 (MQ=255)
                                                                       tttttCGCTTTCGCAAAATGCGCCAGTATGGTCAACCAAAGAACCGAGGGATGTGCGTTCTATGCCCATGTAGGTGCGGACCAGATTTTCACCATAGTAGTCATGGTACAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGccc                                                                      <  1:107643/148‑1 (MQ=255)
                                                                         tttcgctttcgcCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCtg                                                                    >  1:1766932/1‑148 (MQ=255)
                                                                         tttcgctttcgcCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCtg                                                                    >  2:3507683/1‑148 (MQ=255)
                                                                              ctttcgcCAAATGCGCCATTATGGTCAAGCAAAGAACGGCGGGAAGTTCTTTCGATGCCCATGTTGGTGCGGAACAGATTTTCACCATAGTAGTATTGGTATAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGc                                                               <  1:3459267/148‑1 (MQ=255)
                                                                                    ccAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGgcg                                                         >  1:1917190/1‑148 (MQ=255)
                                                                                    ccAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGgcg                                                         >  1:2439505/1‑148 (MQ=255)
                                                                                     cAACTGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGgcgc                                                        <  2:3081269/148‑1 (MQ=255)
                                                                                     cAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGgcgc                                                        <  2:276487/148‑1 (MQ=255)
                                                                                     cAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGgcgc                                                        <  1:2066646/148‑1 (MQ=255)
                                                                                        aTGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGc                                                     >  1:2750937/1‑148 (MQ=255)
                                                                                           cgcCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCa                                                  <  2:3000773/148‑1 (MQ=255)
                                                                                              cAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGa                                               >  1:1936294/1‑148 (MQ=255)
                                                                                                 tATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAata                                            >  1:2617121/1‑148 (MQ=255)
                                                                                                 tATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAata                                            >  2:2591140/1‑148 (MQ=255)
                                                                                                 tATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAata                                            >  1:972432/1‑148 (MQ=255)
                                                                                                    ggacAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTc                                         <  1:1723285/145‑1 (MQ=255)
                                                                                                    ggTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCAATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTc                                         <  1:1723308/148‑1 (MQ=255)
                                                                                                        aaGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCCACGCCGCCCGGGTGGCCTGGCGCTGCCCAGGAATATTCATgg                                     >  2:2170983/1‑148 (MQ=255)
                                                                                                         aGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGa                                    >  1:1103109/1‑148 (MQ=255)
                                                                                                         aGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGa                                    <  2:79190/148‑1 (MQ=255)
                                                                                                         aGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGa                                    >  1:1103010/1‑148 (MQ=255)
                                                                                                          gCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGAt                                   >  1:507505/1‑148 (MQ=255)
                                                                                                           cAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATg                                  >  1:2859515/1‑148 (MQ=255)
                                                                                                            aaaGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGt                                 <  2:1683088/148‑1 (MQ=255)
                                                                                                            aaaGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGt                                 <  2:1683106/148‑1 (MQ=255)
                                                                                                             aaGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGACTATTCATGGAGGTc                                >  2:2610259/1‑148 (MQ=255)
                                                                                                             aaGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTc                                <  2:2671794/148‑1 (MQ=255)
                                                                                                             aaGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTc                                <  2:141976/148‑1 (MQ=255)
                                                                                                              aGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCa                               >  1:1072880/1‑148 (MQ=255)
                                                                                                              aGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCa                               <  2:2230024/148‑1 (MQ=255)
                                                                                                                aaCCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACt                             >  2:1056616/1‑148 (MQ=255)
                                                                                                                  ccGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACtat                           >  2:340153/1‑148 (MQ=255)
                                                                                                                  ccGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACtat                           >  2:340156/1‑148 (MQ=255)
                                                                                                                  ccGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACtat                           >  1:2652391/1‑148 (MQ=255)
                                                                                                                  ccGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACtat                           >  1:2652375/1‑148 (MQ=255)
                                                                                                                   cGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTATAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGTATGTCACtata                          <  2:927158/148‑1 (MQ=255)
                                                                                                                    gAGGGAAGTTCGTTCGATGCCCATGTTGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTatat                         <  1:1651001/148‑1 (MQ=255)
                                                                                                                    gAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTatat                         <  1:1650675/148‑1 (MQ=255)
                                                                                                                      gggAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCCGGACTATTCATGGATGTCACTCTAttt                       >  2:1369587/1‑148 (MQ=255)
                                                                                                                         aaGTTCGTTCTATGCCAATGTAGGTGCGTAAAAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCATGAATATTCATGGATGTCACTATATTtcat                    <  2:1026733/148‑1 (MQ=255)
                                                                                                                         aaGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATCGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTtcat                    <  2:1026760/148‑1 (MQ=255)
                                                                                                                         aaGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTtcat                    <  1:2298694/148‑1 (MQ=255)
                                                                                                                                 tcgATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAgcgcgc            >  1:1834990/1‑148 (MQ=255)
                                                                                                                                 tcgATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAgcgcgc            >  1:1186398/1‑148 (MQ=255)
                                                                                                                                  cgATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCt           >  1:1229542/1‑148 (MQ=255)
                                                                                                                                      gCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCTGAAc       <  1:2909133/148‑1 (MQ=255)
                                                                                                                                       cccATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCTGAACa      <  1:3507683/148‑1 (MQ=255)
                                                                                                                                       cccATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCTGAACa      <  2:612499/148‑1 (MQ=255)
                                                                                                                                        ccATGTCGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCTGAACAg     <  2:1664510/148‑1 (MQ=255)
                                                                                                                                         cATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCTGAACAGt    >  1:3185837/1‑148 (MQ=255)
                                                                                                                                          aTGTCGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCTGAACAGTg   <  2:535604/148‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                
GTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTCGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCTGAACAGTGG  >  CP009273/4329632‑4329918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑