Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,337,927 A→T 7.3% V212E (GTA→GAA)  dcuB ← C4‑dicarboxylate transporter, anaerobic; DcuS co‑sensor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,337,9270AT 7.3% 670.0 / 32.2 217V212E (GTA→GAA) dcuBC4‑dicarboxylate transporter, anaerobic; DcuS co‑sensor
Reads supporting (aligned to +/- strand):  ref base A (78/123);  new base T (5/11);  total (83/134)
Fisher's exact test for biased strand distribution p-value = 6.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 219 total aligned reads displayed.

GATGGACGCAGGTCCGAATCAGCACCAAGAAGGGCGACTACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGATCGAGGAACTCAAGATGGCGACCATCAAAG  >  CP009273/4337780‑4338073
                                                                                                                                                   |                                                                                                                                                  
gATGGACGCAGGTCCGAATCAGCACCAAGAAGGGCGACTACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTa                                                                                                                                                    <  2:2068162/148‑1 (MQ=255)
gATGGACGCAGGTCCGAATCAGCACCAAGAAGGGCGACTACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTa                                                                                                                                                    <  2:272810/148‑1 (MQ=255)
  tGGACGCAGGTCCGAATCAGCACCAAGAAGGGCGACTACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACg                                                                                                                                                  <  2:1794296/148‑1 (MQ=255)
  tGGACGCAGGTCCGAATCAGCACCAAGAAGGGCGACTACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACg                                                                                                                                                  >  1:829087/1‑148 (MQ=255)
   ggACGCAGGTCCGAATCAGCACCAAGAAGGGCGACTACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACgg                                                                                                                                                 <  1:267756/148‑1 (MQ=255)
   ggACGCAGGTCCGAATCAGCACCAAGAAGGGCGACTACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACgg                                                                                                                                                 <  1:1870423/148‑1 (MQ=255)
        cAGGTCCGAATCAGCACCAAGAAGGGCGACTACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATg                                                                                                                                            <  1:1000984/148‑1 (MQ=255)
         aGGTCCGAATCAGCACCAAGAAGGGCGACTACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGa                                                                                                                                           <  1:3289465/148‑1 (MQ=255)
         aGGTCCGAATCAGCACCAAGAAGGGCGACTACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGa                                                                                                                                           >  2:2386534/1‑148 (MQ=255)
          ggTCCGAATCAGCACCAAGAAGGGCGACTACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGaa                                                                                                                                          <  2:2427953/148‑1 (MQ=255)
             ccGAATCAGCACCAAGAAGGGCGACTACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAAttt                                                                                                                                       <  1:1634621/148‑1 (MQ=255)
             ccGAATCAGCACCAAGAAGGGCGACTACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAAttt                                                                                                                                       <  2:3059748/148‑1 (MQ=255)
              cGAATCAGCACCAAGAAGGGCGACTACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTc                                                                                                                                      <  2:3425976/148‑1 (MQ=255)
                 aTCAGCACCAAGAAGGGCGACTACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTgg                                                                                                                                   <  2:2144824/148‑1 (MQ=255)
                 aTCAGCACCAAGAAGGGCGACTACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTgg                                                                                                                                   <  2:1583531/148‑1 (MQ=255)
                  tCAGCACCAAGAAGGGCGACTACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGa                                                                                                                                  >  1:1141586/1‑148 (MQ=255)
                   cAGCACCAAGAAGGGCGACTACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGaa                                                                                                                                 >  2:384517/1‑148 (MQ=255)
                      cACCAAGAAGGGCGACTACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTTCGGAGATGAATTTCTGGAActc                                                                                                                              <  2:1167776/148‑1 (MQ=255)
                      cACCAAGAAGGGCGACTACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTTCGGAGATGAATTTCTGGAActc                                                                                                                              <  2:1168164/148‑1 (MQ=255)
                         cAAGAAGGGCGACTACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTc                                                                                                                           >  1:235831/1‑148 (MQ=255)
                           agaagGGCGACTACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGt                                                                                                                         >  2:2279153/1‑148 (MQ=255)
                             aagGGCGACTACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTTCGGAGATGAATTTCTGGAACTCTTCGTCt                                                                                                                       <  2:3468396/148‑1 (MQ=255)
                             aagGGCGACTACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCt                                                                                                                       >  2:846784/1‑148 (MQ=255)
                              agGGCGACTACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCtt                                                                                                                      <  2:2533021/148‑1 (MQ=255)
                              agGGCGACTACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCtt                                                                                                                      <  2:3496287/148‑1 (MQ=255)
                                 gCGACTACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTAt                                                                                                                   >  1:170794/1‑148 (MQ=255)
                                  cGACTACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATc                                                                                                                  <  1:3205117/148‑1 (MQ=255)
                                      tACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGa                                                                                                              <  1:2094868/148‑1 (MQ=255)
                                      tACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGa                                                                                                              <  2:2692232/148‑1 (MQ=255)
                                        cAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATc                                                                                                            <  2:671044/148‑1 (MQ=255)
                                         aGCGAGTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCt                                                                                                           <  2:1976256/148‑1 (MQ=255)
                                          gCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCtt                                                                                                          <  1:2345543/148‑1 (MQ=255)
                                           cGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCttt                                                                                                         <  1:2524903/148‑1 (MQ=255)
                                           cGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCttt                                                                                                         <  2:1650479/148‑1 (MQ=255)
                                            gATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTa                                                                                                        <  2:2851653/148‑1 (MQ=255)
                                              ttGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTAcc                                                                                                      >  1:525672/1‑148 (MQ=255)
                                                 ccccGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACcgcg                                                                                                   >  1:843244/1‑148 (MQ=255)
                                                 ccccGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACcgcg                                                                                                   >  1:1427004/1‑148 (MQ=255)
                                                    cGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGaa                                                                                                <  2:2730702/148‑1 (MQ=255)
                                                     gAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAAc                                                                                               <  2:2818167/148‑1 (MQ=255)
                                                     gAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAAc                                                                                               <  2:1667955/148‑1 (MQ=255)
                                                       ggAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTTCGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCa                                                                                             <  1:2243472/148‑1 (MQ=255)
                                                       ggAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCa                                                                                             <  1:2837165/148‑1 (MQ=255)
                                                       ggAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCa                                                                                             <  1:3011752/148‑1 (MQ=255)
                                                        gAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAg                                                                                            >  2:1844229/1‑148 (MQ=255)
                                                         aaaaTCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGc                                                                                           <  1:3118518/148‑1 (MQ=255)
                                                          aaaTCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCt                                                                                          >  1:1550182/1‑148 (MQ=255)
                                                             tCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGaa                                                                                       >  2:1762838/1‑148 (MQ=255)
                                                               cacaTTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGa                                                                                     <  1:2573552/148‑1 (MQ=255)
                                                                 caTTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATa                                                                                   >  1:2810632/1‑148 (MQ=255)
                                                                 caTTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATa                                                                                   >  2:1066362/1‑148 (MQ=255)
                                                                  aTTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATAc                                                                                  <  2:1243871/148‑1 (MQ=255)
                                                                     gccagccaGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGa                                                                               <  1:2077419/148‑1 (MQ=255)
                                                                      ccagccagTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGAt                                                                              <  1:2384382/148‑1 (MQ=255)
                                                                           cagTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCa                                                                         <  1:529973/148‑1 (MQ=255)
                                                                             gTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAgg                                                                       <  1:750647/148‑1 (MQ=255)
                                                                             gTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAgg                                                                       >  1:654817/1‑148 (MQ=255)
                                                                             gTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAgg                                                                       >  1:147499/1‑148 (MQ=255)
                                                                             gTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAgg                                                                       <  1:3446902/148‑1 (MQ=255)
                                                                                gCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATa                                                                    <  2:2872071/148‑1 (MQ=255)
                                                                                  ttttCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATAcc                                                                  >  1:621295/1‑148 (MQ=255)
                                                                                   tttCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCg                                                                 >  2:2591854/1‑148 (MQ=255)
                                                                                     tCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGAt                                                               <  1:563513/148‑1 (MQ=255)
                                                                                      cGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGAtt                                                              >  2:3388410/1‑148 (MQ=255)
                                                                                       ggCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTa                                                             <  1:2034048/148‑1 (MQ=255)
                                                                                          aGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTTCGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACg                                                          <  2:3316454/148‑1 (MQ=255)
                                                                                          aGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACg                                                          <  1:429041/148‑1 (MQ=255)
                                                                                           gTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGt                                                         >  1:1934449/1‑148 (MQ=255)
                                                                                             tttttATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCg                                                       >  1:1163015/1‑148 (MQ=255)
                                                                                              ttttATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGa                                                      <  2:2637520/148‑1 (MQ=255)
                                                                                                  aTCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGa                                                  <  2:1085498/148‑1 (MQ=255)
                                                                                                    ccAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAAt                                                >  1:1776248/1‑148 (MQ=255)
                                                                                                     cagcagCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATg                                               <  2:928997/148‑1 (MQ=255)
                                                                                                      agcagcGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGTAATgg                                              <  2:46765/148‑1 (MQ=255)
                                                                                                       gcagcGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTTCGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGt                                             <  2:1390462/148‑1 (MQ=255)
                                                                                                        cagcGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTTCGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTg                                            >  1:1827135/1‑148 (MQ=255)
                                                                                                         agcGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGa                                           <  1:1589105/148‑1 (MQ=255)
                                                                                                         agcGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGa                                           <  1:3336045/148‑1 (MQ=255)
                                                                                                          gcGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGAt                                          >  2:186065/1‑148 (MQ=255)
                                                                                                           cGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGAtt                                         >  1:1194408/1‑148 (MQ=255)
                                                                                                                 ggTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCcagc                                   <  1:2690777/148‑1 (MQ=255)
                                                                                                                   tATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCcagcag                                 <  1:2945835/148‑1 (MQ=255)
                                                                                                                    aTCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGa                                >  1:810229/1‑148 (MQ=255)
                                                                                                                      cACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGATc                              >  2:2143657/1‑148 (MQ=255)
                                                                                                                         cGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGATCGAg                           >  1:109791/1‑148 (MQ=255)
                                                                                                                           tAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGATCGAGGa                         >  1:3331080/1‑148 (MQ=255)
                                                                                                                            aaaCATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGATCGAGGaa                        <  1:84425/148‑1 (MQ=255)
                                                                                                                            aaaCATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACAGATCTAAAGGATACAGATTAACGTCGATGTAATGGTGATTGCCAGAAGATATAGGaa                        >  2:241074/1‑148 (MQ=255)
                                                                                                                              aCATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGATCGAGGAACt                      <  2:2663611/148‑1 (MQ=255)
                                                                                                                                  aCTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGATCGAGGAACTCAAg                  <  1:2282830/148‑1 (MQ=255)
                                                                                                                                   cTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGATCGAGGAACTCAAGa                 <  2:235831/148‑1 (MQ=255)
                                                                                                                                          ttttCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGATCGAGGAACTCAAGATGGCGAc          >  2:1490067/1‑148 (MQ=255)
                                                                                                                                              ccGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGATCGAGGAACTCAAGATGGCGACCATc      >  1:908309/1‑148 (MQ=255)
                                                                                                                                              ccGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGATCGAGGAACTCAAGATGGCGACCATc      >  1:1313235/1‑148 (MQ=255)
                                                                                                                                                ggTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGATCGAGGAACTCAAGATGGCGACCATCaa    <  2:1816906/148‑1 (MQ=255)
                                                                                                                                                 gTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGATCGAGGAACTCAAGATGGCGACCATCaaa   >  2:3223431/1‑148 (MQ=255)
                                                                                                                                                 gTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGATCGAGGAACTCAAGATGGCGACCATCaaa   >  1:2929362/1‑148 (MQ=255)
                                                                                                                                                 gTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGATCGAGGAACTCAAGATGGCGACCATCaaa   <  1:186058/148‑1 (MQ=255)
                                                                                                                                                  tACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGATCGAGGAACTCAAGATGGCGACCATCAAAg  <  1:3381156/148‑1 (MQ=255)
                                                                                                                                                  tACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGATCGAGGAACTCAAGATGGCGACCATCAAAg  <  1:339635/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GATGGACGCAGGTCCGAATCAGCACCAAGAAGGGCGACTACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGATCGAGGAACTCAAGATGGCGACCATCAAAG  >  CP009273/4337780‑4338073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑