Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,402,850 G→T 100% L2L (CTG→CTT)  yjfC → ATP‑Grasp family ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,402,8500GT98.6% 466.5 / ‑1.5 145L2L (CTG→CTT) yjfCATP‑Grasp family ATPase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (79/64);  total (80/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 146 total aligned reads displayed.

GGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAGATCGA  >  CP009273/4402705‑4402993
                                                                                                                                                 |                                                                                                                                               
ggTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTtag                                                                                                                                               >  1:1900056/1‑148 (MQ=255)
   gATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCCTTGGGGAGGCTAATCATGCTTAGaca                                                                                                                                            >  2:1061440/1‑148 (MQ=255)
   gATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGaca                                                                                                                                            >  2:600145/1‑148 (MQ=255)
   gATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGaca                                                                                                                                            >  2:2836713/1‑148 (MQ=255)
   gATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGaca                                                                                                                                            >  2:2903357/1‑148 (MQ=255)
     ttACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCAGTGGGGAGGCTAATCATGCTTAGAcaca                                                                                                                                          >  1:1867239/1‑148 (MQ=255)
      tACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACaa                                                                                                                                         >  2:2058608/1‑148 (MQ=255)
      tACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCAGTGGGGAGGCTAATCATGCTTAGACACaa                                                                                                                                         >  1:1487864/1‑148 (MQ=255)
           cTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTc                                                                                                                                    <  1:743079/148‑1 (MQ=255)
              gcgcTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCtg                                                                                                                                 <  1:1169812/148‑1 (MQ=255)
              gcgcTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCtg                                                                                                                                 <  1:1170551/148‑1 (MQ=255)
              gcgcTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCtg                                                                                                                                 >  2:1590701/1‑148 (MQ=255)
               cgcTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCtgt                                                                                                                                >  2:2512928/1‑148 (MQ=255)
                   ccGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGa                                                                                                                            <  1:615100/148‑1 (MQ=255)
                     ggTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACg                                                                                                                          <  2:1526242/148‑1 (MQ=255)
                      gtacTGGCAAAAAAGAGTCTTACTCTTCGGGCGCCTTAAACACCAAAAAAGCGTCTACAGTTTCTCTCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGCATGCTAATCATACTTAGAAAAAACGTTCATGTGCGACgc                                                                                                                         >  2:322946/4‑147 (MQ=255)
                      gTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTAACACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCAGTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACgg                                                                                                                         >  2:424020/1‑148 (MQ=255)
                        tCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCCTTGGGGAGGCTAACCATGCTTAGACACAACGTTCCTGTGCGACGGGa                                                                                                                       >  2:18452/1‑148 (MQ=255)
                        tCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGa                                                                                                                       >  2:18449/1‑148 (MQ=255)
                        tCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGa                                                                                                                       >  2:2989111/1‑148 (MQ=255)
                           ggCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCt                                                                                                                    <  1:2264529/148‑1 (MQ=255)
                            gCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTg                                                                                                                   >  2:1436361/1‑148 (MQ=255)
                            gCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTg                                                                                                                   >  2:2002674/1‑148 (MQ=255)
                               aaaaaGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCCTTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGAc                                                                                                                >  1:2083942/1‑148 (MQ=255)
                                aaaaGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCCTTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGAcc                                                                                                               >  2:361676/1‑148 (MQ=255)
                                 aaaGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCa                                                                                                              >  2:1223274/1‑148 (MQ=255)
                                       tCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCAGTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATcgc                                                                                                        >  2:2039201/1‑148 (MQ=255)
                                             tctTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGa                                                                                                  <  1:701971/148‑1 (MQ=255)
                                              ctTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGATATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGAc                                                                                                 >  2:3447831/1‑148 (MQ=255)
                                                   ggcggcTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACgg                                                                                            <  1:2421279/148‑1 (MQ=255)
                                                       gcTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCGGACAACGGTTTc                                                                                        >  1:2788009/1‑148 (MQ=255)
                                                        cTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGGCTTGGGGCGGCTAATAATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATAGCCGCTGACAACGGTTTCg                                                                                       >  1:1294569/1‑148 (MQ=255)
                                                        cTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCCTTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCg                                                                                       >  2:2959762/1‑148 (MQ=255)
                                                        cTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCg                                                                                       >  1:502639/1‑148 (MQ=255)
                                                        cTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCAGTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCg                                                                                       >  1:502729/1‑148 (MQ=255)
                                                         tttACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGa                                                                                      >  2:1054283/1‑148 (MQ=255)
                                                          ttACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGAc                                                                                     <  2:3275868/148‑1 (MQ=255)
                                                           tACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACt                                                                                    >  1:824095/1‑148 (MQ=255)
                                                           tACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACt                                                                                    <  1:1379337/148‑1 (MQ=255)
                                                           tACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACt                                                                                    <  1:3149659/148‑1 (MQ=255)
                                                             ccaccaAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATcgccg                                                                                                      <  2:3508165/128‑1 (MQ=255)
                                                              caccaAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTc                                                                                 <  1:2736556/148‑1 (MQ=255)
                                                              caccaAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTc                                                                                 <  1:2736508/148‑1 (MQ=255)
                                                              caccaAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTc                                                                                 <  1:1528825/148‑1 (MQ=255)
                                                               accaAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCa                                                                                <  1:3281037/148‑1 (MQ=255)
                                                                ccaAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCat                                                                               <  1:2842582/148‑1 (MQ=255)
                                                                  aaaaaaGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTACAGCGCCCGTGGGGATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGGCCAGATCGCCGCTGACAACGGTTTCGACTTTCatat                                                                             >  1:154428/1‑148 (MQ=255)
                                                                     aaaGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCCTTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGCCCAGATCGCCGCTGACCACGGTTTCGACTTTCATatcat                                                                          >  2:732098/1‑148 (MQ=255)
                                                                     aaaGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATatcat                                                                          <  2:734454/148‑1 (MQ=255)
                                                                       aGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCg                                                                        <  1:1499705/148‑1 (MQ=255)
                                                                       aGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCg                                                                        <  1:2399783/148‑1 (MQ=255)
                                                                         cGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCCTTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGATAACGGTTTCGACTTTCATATCATCGAc                                                                      >  1:2085119/1‑148 (MQ=255)
                                                                            cTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCCTTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAAt                                                                   >  1:2505689/1‑148 (MQ=255)
                                                                            cTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACaaa                                                                   >  1:2211074/1‑147 (MQ=255)
                                                                            cTACCGTTTCTCGCGGCGGCTATGGTCGTTCATGCAGCGCCCGGGGGGATTGGGGGGGCTAAAAAAGCTTAGACACAACCTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCGGACAACGGTTTAGACTTTAATATAAACGACaaa                                                                   >  1:1557402/1‑147 (MQ=255)
                                                                               ccGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGaa                                                                <  1:3431645/148‑1 (MQ=255)
                                                                               ccGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGaa                                                                <  1:3431121/148‑1 (MQ=255)
                                                                               ccGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGaa                                                                <  2:2548680/148‑1 (MQ=255)
                                                                                     ctcGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTAt                                                          <  1:3522583/148‑1 (MQ=255)
                                                                                     ctcGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTAt                                                          <  1:1871704/148‑1 (MQ=255)
                                                                                     ctcGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTAt                                                          <  2:2766279/148‑1 (MQ=255)
                                                                                         cggcggCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTggg                                                      <  2:1156007/148‑1 (MQ=255)
                                                                                         cggcggCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTggg                                                      >  2:3095388/1‑148 (MQ=255)
                                                                                         cggcggCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTggg                                                      <  2:2302705/148‑1 (MQ=255)
                                                                                           gcggcTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGAt                                                    <  1:339408/148‑1 (MQ=255)
                                                                                           gcggcTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGAt                                                    <  1:337630/148‑1 (MQ=255)
                                                                                              gcTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTAGACTTTCATATCATCGACAATGAAATCTATTGGGATgag                                                 <  1:153954/148‑1 (MQ=255)
                                                                                                tATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGagag                                               <  2:2585576/148‑1 (MQ=255)
                                                                                                tATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGagag                                               <  1:973554/148‑1 (MQ=255)
                                                                                                 aTGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTTCGACGGGATCTTGAACAGATCGCCGCTGACAACGGTTTAGACTTTAATATCAACGACAAAGAAAACTAATGGGATGAGAGt                                              >  2:401354/1‑148 (MQ=255)
                                                                                                 aTGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGt                                              >  2:402492/1‑148 (MQ=255)
                                                                                                        ttcttcCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCtt                                       >  2:1973898/1‑148 (MQ=255)
                                                                                                         tcttcCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTa                                      >  2:1365312/1‑148 (MQ=255)
                                                                                                             ccAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGt                                  >  1:928939/1‑148 (MQ=255)
                                                                                                               aGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGttt                                >  2:1883029/1‑148 (MQ=255)
                                                                                                               aGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGttt                                <  1:1681684/148‑1 (MQ=255)
                                                                                                               aGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGttt                                <  1:1223274/148‑1 (MQ=255)
                                                                                                                gcgcCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGtttt                               >  2:2014625/1‑148 (MQ=255)
                                                                                                                 cgcCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTa                              >  2:2338483/1‑148 (MQ=255)
                                                                                                                 cgcCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTa                              >  1:2286002/1‑148 (MQ=255)
                                                                                                                 cgcCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTa                              >  1:3253027/1‑148 (MQ=255)
                                                                                                                    ccGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTActc                           <  2:3012189/148‑1 (MQ=255)
                                                                                                                    ccGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTActc                           <  2:357003/148‑1 (MQ=255)
                                                                                                                         ggCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTgcgc                      <  1:646152/148‑1 (MQ=255)
                                                                                                                          gCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGcc                     <  2:1628875/148‑1 (MQ=255)
                                                                                                                                 ggAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGa              >  2:2714029/1‑148 (MQ=255)
                                                                                                                                  gAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGaa             <  1:2198221/148‑1 (MQ=255)
                                                                                                                                   aGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAag            <  2:499801/148‑1 (MQ=255)
                                                                                                                                   aGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAag            >  2:1856214/1‑148 (MQ=255)
                                                                                                                                      cTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGc         <  1:1590701/148‑1 (MQ=255)
                                                                                                                                       tAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCa        <  2:2454206/148‑1 (MQ=255)
                                                                                                                                        aaTCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAg       <  1:1117630/148‑1 (MQ=255)
                                                                                                                                        aaTCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAg       >  2:362201/1‑148 (MQ=255)
                                                                                                                                         aTCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAGa      <  2:1487864/148‑1 (MQ=255)
                                                                                                                                           cATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAGATc    >  1:1881757/1‑148 (MQ=255)
                                                                                                                                           cATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAGATc    >  1:3388272/1‑148 (MQ=255)
                                                                                                                                            aTGCTTAGACACAACGTTCCTGTGCGACGGTATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAGATCg   <  1:2951759/148‑1 (MQ=255)
                                                                                                                                            aTGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAGATCg   <  1:3542725/148‑1 (MQ=255)
                                                                                                                                             tGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAGATCGa  >  2:575814/1‑148 (MQ=255)
                                                                                                                                             tGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAGATCGa  >  2:2362013/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
GGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAGATCGA  >  CP009273/4402705‑4402993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑