Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,410,964 A→C 100% I390L (ATC→CTC)  ulaA → L‑ascorbate‑specific enzyme IIC permease component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,410,9640AC95.7% 794.6 / 8.1 208I390L (ATC→CTC) ulaAL‑ascorbate‑specific enzyme IIC permease component of PTS
Reads supporting (aligned to +/- strand):  ref base A (5/4);  new base C (124/75);  total (129/79)
Fisher's exact test for biased strand distribution p-value = 7.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 212 total aligned reads displayed.

TGGCATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAA  >  CP009273/4410819‑4411108
                                                                                                                                                 |                                                                                                                                                
  gCATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGa                                                                                                                                              >  1:2825838/1‑148 (MQ=255)
  gCATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGa                                                                                                                                              >  1:2163191/1‑148 (MQ=255)
   cATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGaa                                                                                                                                             >  2:2322731/1‑148 (MQ=255)
      ccTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATc                                                                                                                                          <  1:2573070/148‑1 (MQ=255)
        tGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCtt                                                                                                                                        <  2:3289405/148‑1 (MQ=255)
         ggTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCttt                                                                                                                                       <  2:795615/148‑1 (MQ=255)
          gTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTTTGTGGTGATGGGGATGCTCGAAATCtttt                                                                                                                                      >  1:317007/1‑147 (MQ=255)
          gTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTg                                                                                                                                      <  2:3150780/148‑1 (MQ=255)
          gTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTg                                                                                                                                      <  1:2590499/148‑1 (MQ=255)
             gCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGtt                                                                                                                                   >  1:2095730/1‑148 (MQ=255)
              ccTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTg                                                                                                                                  >  1:2067561/1‑148 (MQ=255)
              ccTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATGTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTg                                                                                                                                  >  2:2673819/1‑148 (MQ=255)
                 gCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGt                                                                                                                               <  2:1994779/148‑1 (MQ=255)
                   ggCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCt                                                                                                                             <  2:542651/148‑1 (MQ=255)
                        ctcGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCg                                                                                                                        >  2:739225/1‑148 (MQ=255)
                        ctcGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCg                                                                                                                        >  2:2057328/1‑148 (MQ=255)
                             tCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGaa                                                                                                                   <  2:2471465/148‑1 (MQ=255)
                             tCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGaa                                                                                                                   >  1:2481836/1‑148 (MQ=255)
                                tGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACt                                                                                                                >  1:1160265/1‑148 (MQ=255)
                                  attattCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCa                                                                                                              <  1:349456/148‑1 (MQ=255)
                                   ttattCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCAc                                                                                                             <  2:1597288/148‑1 (MQ=255)
                                    tattCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCAcc                                                                                                            >  2:2263309/1‑148 (MQ=255)
                                      ttCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCgg                                                                                                          >  2:3045570/1‑148 (MQ=255)
                                      ttCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGGTGCGTCTGGGCGGTGAAACTCACCgg                                                                                                          >  1:1856454/1‑148 (MQ=255)
                                       tCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGt                                                                                                         >  1:1410044/1‑148 (MQ=255)
                                          tGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATg                                                                                                      >  2:2770464/1‑148 (MQ=255)
                                            gCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAg                                                                                                    <  2:1257130/148‑1 (MQ=255)
                                             cTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCCAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGt                                                                                                   >  2:111760/1‑148 (MQ=255)
                                              tttATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTg                                                                                                  >  2:2568245/1‑148 (MQ=255)
                                              tttATCCCGATGTTCTTATCTAACGCCACCATCGGCGTGTTAGCTAACAACTTCGGCGGCTGGCGTGCCGCTCTGAAGATTTGTCTGGTTATGGGTATGCTCGAAATATTTGGTTGCGTCTGGGCGGTGAAACTAACCGGTATGAGTg                                                                                                  >  2:308027/1‑148 (MQ=255)
                                               ttATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATTCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGc                                                                                                 >  1:1260906/1‑148 (MQ=255)
                                               ttATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGc                                                                                                 <  2:1794080/148‑1 (MQ=255)
                                               ttATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGc                                                                                                 <  2:2855990/148‑1 (MQ=255)
                                                  tCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTg                                                                                              >  2:1276055/1‑148 (MQ=255)
                                                       aTGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTGTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATgg                                                                                         >  1:1242013/1‑148 (MQ=255)
                                                       aTGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATgg                                                                                         >  2:2095094/1‑148 (MQ=255)
                                                       aTGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATgg                                                                                         >  2:301148/1‑148 (MQ=255)
                                                        tGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATggg                                                                                        >  2:3346305/1‑148 (MQ=255)
                                                        tGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATggg                                                                                        >  2:1783798/1‑148 (MQ=255)
                                                         gTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGc                                                                                       <  1:482107/148‑1 (MQ=255)
                                                          ttcttcTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCa                                                                                      >  1:1342899/1‑148 (MQ=255)
                                                          ttcttcTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCa                                                                                      >  1:3470552/1‑148 (MQ=255)
                                                           tcttctCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCAt                                                                                     >  2:679279/1‑148 (MQ=255)
                                                            cttctCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGTGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATg                                                                                    >  1:975752/1‑148 (MQ=255)
                                                               ctctAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCg                                                                                 >  2:3030422/1‑148 (MQ=255)
                                                                 ctAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGa                                                                               <  1:2379159/148‑1 (MQ=255)
                                                                     cGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGACATCTTTGGTTGCGTCTGGGCGGTGATACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTgg                                                                           <  1:1555563/148‑1 (MQ=255)
                                                                         accaTCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGa                                                                       >  2:266957/1‑148 (MQ=255)
                                                                         accaTCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGa                                                                       <  2:2177455/148‑1 (MQ=255)
                                                                         accaTCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGa                                                                       <  1:663716/148‑1 (MQ=255)
                                                                         accaTCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCGTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCCTGGCGGACTGGTCGa                                                                       >  2:1216620/1‑148 (MQ=255)
                                                                          ccaTCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGAt                                                                      <  1:1749976/148‑1 (MQ=255)
                                                                           caTCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGAtt                                                                     >  1:3403481/1‑148 (MQ=255)
                                                                             tCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCt                                                                   >  2:1184941/1‑148 (MQ=255)
                                                                              cGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTg                                                                  >  2:422956/1‑148 (MQ=255)
                                                                                     ttCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGcgccgc                                                           >  1:27574/1‑148 (MQ=255)
                                                                                     ttCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCAccgc                                                           >  1:440665/1‑148 (MQ=255)
                                                                                      tCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCAccgcc                                                          >  1:64763/1‑148 (MQ=255)
                                                                                          tAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCgatg                                                      >  1:2914129/1‑148 (MQ=255)
                                                                                            aCCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCgatgat                                                    >  1:913392/1‑148 (MQ=255)
                                                                                            aCCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCgatgat                                                    >  1:2693395/1‑148 (MQ=255)
                                                                                                cTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCaa                                                >  1:1103517/1‑148 (MQ=255)
                                                                                                     gcggcTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGctt                                           >  2:1412368/1‑148 (MQ=255)
                                                                                                       ggcTGGCGTGCGGCGCTGAAGTTTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGcttct                                         <  1:279585/148‑1 (MQ=255)
                                                                                                       ggcTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGcttct                                         <  1:2019904/148‑1 (MQ=255)
                                                                                                        gcTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGcttctt                                        >  2:401008/1‑148 (MQ=255)
                                                                                                        gcTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGcttctt                                        <  1:2178799/148‑1 (MQ=255)
                                                                                                        gcTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGcttctt                                        <  1:2179039/148‑1 (MQ=255)
                                                                                                            gCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTcc                                    >  2:2915096/1‑148 (MQ=255)
                                                                                                              gTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCAt                                  <  2:1456599/148‑1 (MQ=255)
                                                                                                              gTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCAt                                  <  2:2126315/148‑1 (MQ=255)
                                                                                                                gcggcgCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCg                                <  1:3509828/148‑1 (MQ=255)
                                                                                                                   gcgcTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTa                             >  1:2113657/1‑148 (MQ=255)
                                                                                                                      cTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCg                          >  2:83598/1‑148 (MQ=255)
                                                                                                                        gAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGcc                        >  1:1782065/1‑148 (MQ=255)
                                                                                                                        gAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGcc                        <  2:3424602/148‑1 (MQ=255)
                                                                                                                         aaGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCt                       <  1:484190/148‑1 (MQ=255)
                                                                                                                         aaGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCt                       >  2:702194/1‑148 (MQ=255)
                                                                                                                         aaGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCt                       <  1:1987796/148‑1 (MQ=255)
                                                                                                                         aaGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCt                       >  2:627957/1‑148 (MQ=255)
                                                                                                                         aaGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCt                       >  2:3312121/1‑148 (MQ=255)
                                                                                                                           gATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCttt                     >  2:2024206/1‑148 (MQ=255)
                                                                                                                            aTTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTa                    >  1:385085/1‑148 (MQ=255)
                                                                                                                              ttGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATg                  >  2:386544/1‑148 (MQ=255)
                                                                                                                                  cTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCg              <  2:483319/148‑1 (MQ=255)
                                                                                                                                    ggTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGtc            >  1:440528/1‑148 (MQ=255)
                                                                                                                                    ggTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGtc            >  2:44129/1‑148 (MQ=255)
                                                                                                                                    ggTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGtc            >  2:1035444/1‑148 (MQ=255)
                                                                                                                                    ggTGATGGGGATGCTCGAAATCTTGGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGtc            <  2:2739435/148‑1 (MQ=255)
                                                                                                                                     gTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGtca           <  1:83598/148‑1 (MQ=255)
                                                                                                                                      tGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGtcat          <  1:261434/148‑1 (MQ=255)
                                                                                                                                       gATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGtcatc         <  1:869436/148‑1 (MQ=255)
                                                                                                                                       gATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGtcatc         >  2:1915089/1‑148 (MQ=255)
                                                                                                                                        aTGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGtcatca        <  1:772156/148‑1 (MQ=255)
                                                                                                                                        aTGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGtcatca        >  1:230421/1‑148 (MQ=255)
                                                                                                                                        aTGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGtcatca        <  1:576529/148‑1 (MQ=255)
                                                                                                                                        aTGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGtcatca        <  1:2896444/148‑1 (MQ=255)
                                                                                                                                             gatgctCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTa   <  1:301148/148‑1 (MQ=255)
                                                                                                                                             gatgctCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTa   >  1:1097337/1‑148 (MQ=255)
                                                                                                                                             gatgctCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTCGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTa   <  1:2095094/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TGGCATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAA  >  CP009273/4410819‑4411108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑