Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,412,099 G→T 5.3% E33* (GAA→TAA)  ulaD → 3‑keto‑L‑gulonate 6‑phosphate decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,412,0990GT 5.3% 722.3 / 11.8 187E33* (GAA→TAA) ulaD3‑keto‑L‑gulonate 6‑phosphate decarboxylase
Reads supporting (aligned to +/- strand):  ref base G (85/92);  new base T (5/5);  total (90/97)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 187 total aligned reads displayed.

ACTGGATCTCATTGACCGCACCAACGCGGCAGCTTAAGAAGGAATTGAACATGTCATTACCGATGTTGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGCTCTACCCGCACAAAATCGTACTGGCAGACGCCAAAATTGCCGATGCAGGCAAAATCCTTTCGCGTATGTGCTTCGAAGCCAACGCT  >  CP009273/4411953‑4412245
                                                                                                                                                  |                                                                                                                                                  
 cTGGATCTCATTGACCGCACCAACGCGGCAGCTTAAGAAGGAATTGAACATGTCATTACCGATGTTGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGaa                                                                                                                                                  <  1:2912488/148‑1 (MQ=255)
  tGGATCTCATTGACCGCACCAACGCGGCAGCTTAAGAAGGAATTGAACATGTCATTACCGATGTTGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAg                                                                                                                                                 <  2:2268428/148‑1 (MQ=255)
  tGGATCTCATTGACCGCACCAACGCGGCAGCTTAAGAAGGAATTGAACATGTCATTACCGATGTTGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAg                                                                                                                                                 <  2:481205/148‑1 (MQ=255)
     aTCTCATTGACCGCACCAACGCGGCAGCTTAAGAAGGAATTGAACATGTCATTACCGATGTTGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTgg                                                                                                                                              >  2:2812131/1‑148 (MQ=255)
      tctcATTGACCGCACCAACGCGGCAGCTTAAGAAGGAATTGAACATGTCATTACCGATGTTGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTggg                                                                                                                                             <  1:1476737/148‑1 (MQ=255)
      tctcATTGACCGAACCAACGCGGCAGCTTAAGAAGGAATTGAACATGTCATTACCGATGTTGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTggg                                                                                                                                             <  1:2331264/148‑1 (MQ=255)
       ctcATTGACCGCACCAACGCGGCAGCTTAAGAAGGAATTGAACATGTCATTACCGATGTTGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGc                                                                                                                                            >  2:2243672/1‑148 (MQ=255)
       ctcATTGACCGCACCAACGCGGCAGCTTAAGAAGGAATTGAACATGTCATTACCGATGTTGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGc                                                                                                                                            <  2:2816127/148‑1 (MQ=255)
       ctcATTGACCGCACCAACGCGGCAGCTTAAGAAGGAATTGAACATGTCATTACCGATGTTGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGc                                                                                                                                            <  2:2817921/148‑1 (MQ=255)
         cATTGACCGCACCAACGCGGCAGCTTAAGAAGGAATTGAACATGTCATTACCGATGTTGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCTAAGTGGGCAc                                                                                                                                          >  1:847853/1‑148 (MQ=255)
         cATTGACCGCACCAACGCGGCAGCTTAAGAAGGAATTGAACATGTCATTACCGATGTTGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCAc                                                                                                                                          >  2:1760839/1‑148 (MQ=255)
           ttGACCGCACCAACGCGGCAGCTTAAGAAGGAATTGAACATGTCATTACCGATGTTGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCa                                                                                                                                        <  1:2649133/148‑1 (MQ=255)
            tGACCGCACCAACGCGGCAGCTTAAGAAGGAATTGAACATGTCATTACCGATGTTGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCAt                                                                                                                                       >  2:2572081/1‑148 (MQ=255)
            tGACCGCACCAACGCGGCAGCTTAAGAAGGAATTGAACATGTCATTACCGATGTTGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCAt                                                                                                                                       <  1:2032113/148‑1 (MQ=255)
            tGACCGCACCAACGCGGCAGCTTAAGAAGGAATTGAACATGTCATTACCGATGTTGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCAt                                                                                                                                       <  2:2663879/148‑1 (MQ=255)
                cGCACCAACGCGGCAGCTTAAGAAGGAATTGAACATGTCATTACCGATGTTGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCtg                                                                                                                                   <  1:3065936/148‑1 (MQ=255)
                 gCACCAACGCGGCAGCTTAAGAAGGAAGTGAACATGTCATTACCGATGTTGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCtgt                                                                                                                                  <  2:2151120/148‑1 (MQ=255)
                    ccAACGCGGCAGCTTAAGAAGGAATTGAACATGTCATTACCGATGTTGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCg                                                                                                                               >  2:2229858/1‑148 (MQ=255)
                     cAACGCGGCAGCTTAAGAAGGAATTGAACATGTCATTACCGATGTTGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCTAAGTGGGCACCATTCTGTGCGt                                                                                                                              <  1:1831975/148‑1 (MQ=255)
                     cAACGCGGCAGCTTAAGAAGGAATTGAACATGTCATTACCGATGTTGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCTAAGTGGGCACCATTCTGTGCGt                                                                                                                              <  1:1832288/148‑1 (MQ=255)
                     cAACGCGGCAGCTTAAGAAGGAATTGAACATGTCATTACCGATGTTGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGt                                                                                                                              <  2:2100589/148‑1 (MQ=255)
                        cgcgGCAGCTTAAGAAGGAATTGAACATGTCATTACCGATGTTGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTCTCGAAGTGGGCACCATTCTGTGCGTggg                                                                                                                           >  2:2644790/1‑148 (MQ=255)
                            gCAGCTTAAGAAGGAATTGAACATGTCATTACCGATGTTGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGaa                                                                                                                       <  2:1611688/148‑1 (MQ=255)
                             cAGCTTAAGAAGGAATTGAACATGTCATTACCGATGTTGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAg                                                                                                                      <  2:3344585/148‑1 (MQ=255)
                             cAGCTTAAGAAGGAATTGAACATGTCATTACCGATGTTGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAg                                                                                                                      >  1:1761096/1‑148 (MQ=255)
                              aGCTTAAGAAGGAATTGAACATGTCATTACCGATGTTGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAgg                                                                                                                     <  1:920162/148‑1 (MQ=255)
                              aGCTTAAGAAGGAATTGAACATGTCATTACCGATGTTGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAgg                                                                                                                     >  2:2669086/1‑148 (MQ=255)
                               gCTTAAGAAGGAATTGAACATGTCATTACCGATGTTGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGgc                                                                                                                    >  2:1099253/1‑148 (MQ=255)
                                cTTAAGAAGGAATTGAACATGTCATTACCGATGTTGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGACACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGACGTGGGCACCATTCTGTGCGTGGGCGAAGgcg                                                                                                                   >  2:1722499/1‑148 (MQ=255)
                                     gaagGAATTGAACATGTCATTACCGATGTTGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGgcgtgcgt                                                                                                              >  1:2131302/1‑148 (MQ=255)
                                      aagGAATTGAACATGTCATTACCGATGTTGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCTAAGTGGGCACCATTCTGTGCGTGGGCGAAGGcgtgcgtg                                                                                                             >  1:1620754/1‑148 (MQ=255)
                                      aagGAATTGAACATGTCATTACCGATGTTGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGcgtgcgtg                                                                                                             >  1:2998421/1‑148 (MQ=255)
                                        ggAATTGAACATGTCATTACCGATGTTGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGtgcgtgcg                                                                                                           >  1:1721177/1‑148 (MQ=255)
                                         gAATTGAACATGTCATTACCGATGTTGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCgg                                                                                                          >  2:3101060/1‑148 (MQ=255)
                                          aaTTGAACATGTCATTACCGATGTTGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGt                                                                                                         <  1:3163595/148‑1 (MQ=255)
                                          aaTTGAACATGTCATTACCGATGTTGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGt                                                                                                         <  1:3164825/148‑1 (MQ=255)
                                             tGAACATGTCATTACCGATGTTGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCg                                                                                                      >  2:626043/1‑148 (MQ=255)
                                                aCATGTCATTACCGATGTTGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGa                                                                                                   >  1:616989/1‑148 (MQ=255)
                                                   tGTCATTACCGATGTTGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCt                                                                                                <  2:1688289/148‑1 (MQ=255)
                                                     tCATTACCGATGTTGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGa                                                                                              >  2:823155/1‑148 (MQ=255)
                                                      cATTACCGATGTTGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGaa                                                                                             >  1:75141/1‑148 (MQ=255)
                                                       aTTACCGATGTTGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGaaa                                                                                            <  1:411622/148‑1 (MQ=255)
                                                           ccGATGTTGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAgcgc                                                                                        >  1:706253/1‑148 (MQ=255)
                                                           ccGATGTTGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAgcgc                                                                                        >  1:2488266/1‑148 (MQ=255)
                                                            cGATGTTGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGct                                                                                       >  1:3329435/1‑148 (MQ=255)
                                                            cGATGTTGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGct                                                                                       <  2:3163371/148‑1 (MQ=255)
                                                            cGATGTTGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGct                                                                                       <  2:447080/148‑1 (MQ=255)
                                                             gATGTTGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGctc                                                                                      <  2:1338140/148‑1 (MQ=255)
                                                              aTGTTGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGctct                                                                                     <  2:475098/148‑1 (MQ=255)
                                                              aTGTTGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGctct                                                                                     <  2:3431104/148‑1 (MQ=255)
                                                                 ttGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGCTCTAcc                                                                                  >  1:383882/1‑148 (MQ=255)
                                                                 ttGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGCTCTAcc                                                                                  >  1:3206769/1‑148 (MQ=255)
                                                                   gCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGCTCTACCCg                                                                                <  1:2972980/148‑1 (MQ=255)
                                                                    cAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGCTCTACCCGc                                                                               >  2:2434798/1‑148 (MQ=255)
                                                                      aGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGCTCTACCCGcac                                                                             >  1:241706/1‑148 (MQ=255)
                                                                         cgcgCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGCTCTACCCGCACaaa                                                                          <  2:2998421/148‑1 (MQ=255)
                                                                           cgcTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGCTCTACCCGCACAAAAt                                                                        <  2:1796390/148‑1 (MQ=255)
                                                                               ggACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCTAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGCTCTACCCGCACAAAATCGTa                                                                    <  1:81571/148‑1 (MQ=255)
                                                                                gACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGCTCTACCCGCACAAAATCGTAc                                                                   <  2:363814/148‑1 (MQ=255)
                                                                                  cAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGCTCTACCCGCACAAAATCGTACTg                                                                 >  1:1173367/1‑148 (MQ=255)
                                                                                    aCCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGCTCTACCCGCACAAAATCGTACTGGc                                                               >  1:1307683/1‑148 (MQ=255)
                                                                                     ccAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCNTTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGCTCTACCCGCACAAAATCGTACTGGCa                                                              >  1:1803518/1‑148 (MQ=255)
                                                                                     ccAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGCTCTACCCGCACAAAATCGTACTGGCa                                                              >  1:1244937/1‑148 (MQ=255)
                                                                                       aGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGCTCTACCTGCACAAAATCGTACTGGCAGa                                                            <  1:168155/148‑1 (MQ=255)
                                                                                            aTGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGCTCTACCCGCACAAAATCGTACTGGCAGACGCCa                                                       >  1:1817180/1‑148 (MQ=255)
                                                                                              ggATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGCTCTACCCGCACAAAATCGTACTGGCAGACGCCaaa                                                     <  2:1393988/148‑1 (MQ=255)
                                                                                              ggATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGCTCTACCCGCACAAAATCGTACTGGCAGACGCCaaa                                                     <  1:2572081/148‑1 (MQ=255)
                                                                                                  aGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGCTCTACCCGCACAAAATCGTACTGGCAGACGCCAAAATTg                                                 <  2:1403318/148‑1 (MQ=255)
                                                                                                   gcgcCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGCTCTACCCGCACAAAATCGTACTGGCAGACGCCAAAATTGc                                                >  2:2057779/1‑148 (MQ=255)
                                                                                                        tACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCTAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGCTCTACCCGCACAAAATCGTACTGGCAGACGCCAAAATTGCCGATg                                           <  2:1377339/148‑1 (MQ=255)
                                                                                                        tACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGCTCTACCCGCACAAAATCGTACTGGCAGACGCCAAAATTGCCGATg                                           <  1:2243670/148‑1 (MQ=255)
                                                                                                            aaaCCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGCTCTACCCGCACAAAATCGTACTGGCAGACGCCAAAATTGCCGATGCAgg                                       <  2:1618308/148‑1 (MQ=255)
                                                                                                             aaCCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGCTCTACCCGCACAAAATCGTACTGGCAGACGCCAAAATTGCCGATGCAGGc                                      >  1:3471364/1‑148 (MQ=255)
                                                                                                                     gCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGCTCTACCCGCACAAAATCGTACTGGCAGACGCCAAAATTGCCGATGCAGGCAAAATCCt                              <  1:1595284/148‑1 (MQ=255)
                                                                                                                     gCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGCTCTACCCGCACAAAATCGTACTGGCAGACGCCAAAATTGCCGATGCAGGCAAAATCCt                              <  1:2395370/148‑1 (MQ=255)
                                                                                                                      ccTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGCTCTACCCGCACAAAATCGTACTGGCAGACGCCAAAATTGCCGATGCAGGCAAAATCCtt                             <  1:2815960/148‑1 (MQ=255)
                                                                                                                      ccTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGCTCTACCCGCACAAAATCGTACTGGCAGACGCCAAAATTGCCGATGCAGGCAAAATCCtt                             <  1:1777112/148‑1 (MQ=255)
                                                                                                                         gATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGCTCTACCCGCACAAAATCGTACTGGCAGACGCCAAAATTGCCGATGCAGGCAAAATCCTTTcg                          >  2:2541348/1‑148 (MQ=255)
                                                                                                                          aTTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGCTCTACCCGCACAAAATCGTACTGGCAGACGCCAAAATTGCCGATGCAGGCAAAATCCTTTcgc                         >  2:1235576/1‑148 (MQ=255)
                                                                                                                          aTTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGCTCTACCCGCACAAAATCGTACTGGCAGACGCCAAAATTGCCGATGCAGGCAAAATCCTTTcgc                         >  2:2872234/1‑148 (MQ=255)
                                                                                                                            tGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGCTCTACCCGCACAAAATCGTACTGGCAGACGCCAAAATTGCCGATGCAGGCAAAATCCTTTCGCGt                       <  2:3422838/148‑1 (MQ=255)
                                                                                                                             gCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGCTCTACCCGCACAAAATCGTACTGGCAGACGCCAAAATTGCCGATGCAGGCAAAATCCTTTCGCGTa                      <  2:1305939/148‑1 (MQ=255)
                                                                                                                                gaagaaGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGCTCTACCCGCACAAAATCGTACTGGCAGACGCCAAAATTGCCGATGCAGGCAAAATCCTTTCGCGTAtgt                   >  1:1542811/1‑148 (MQ=255)
                                                                                                                                 aagaagTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGCTCTACCCGCACAAAATCGTACTGGCAGACGCCAAAATTGCCGATGCAGGCAAAATCCTTTCGCGTAtgtg                  <  2:74665/148‑1 (MQ=255)
                                                                                                                                 aagaagTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGCTCTACCCGCACAAAATCGTACTGGCAGACGCCAAAATTGCCGATGCAGGCAAAATCCTTTCGCGTAtgtg                  >  2:936225/1‑148 (MQ=255)
                                                                                                                                  agaagTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGCTCTACCCGCACAAAATCGTACTGGCAGACGCCAAAATTGCCGATGCAGGCAAAATCCTTTCGCGTATGTGc                 <  2:267617/148‑1 (MQ=255)
                                                                                                                                  agaagTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGCTCTACCCGCACAAAATCGTACTGGCAGACGCCAAAATTGCCGATGCAGGCAAAATCCTTTCGCGTATGTGc                 >  2:533517/1‑148 (MQ=255)
                                                                                                                                    aagTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGCTCTACCCGCACAAAATCGTACTGGCAGACGCCAAAATTGCCGATGCAGGCAAAATCCTTTCGCGTATGTGCtt               <  1:2847685/148‑1 (MQ=255)
                                                                                                                                    aagTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGCTCTACCCGCACAAAATCGTACTGGCAGACGCCAAAATTGCCGATGCAGGCAAAATCCTTTCGCGTATGTGCtt               >  1:105432/1‑148 (MQ=255)
                                                                                                                                     agTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGCTCTACCCGCACAAAATCGTACTGGCAGACGCCAAAATTGCCGATGCAGGCAAAATCCTTTCGCGTATGTGCTTc              >  2:2369629/1‑148 (MQ=255)
                                                                                                                                      gTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGCTCTACCCGCACAAAATCGTACTGGCAGACGCCAAAATTGCCGATGCAGGCAAAATCCTTTCGCGTATGTGCTTCg             >  1:2289868/1‑148 (MQ=255)
                                                                                                                                       tCGACATGATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGCTCTACCCGCACAAAATCGTACTGGCAGACGCCAAAATTGCCGATGCAGGCAAAATCCTTTCGCGTATGTGCTTCGa            <  1:2812121/148‑1 (MQ=255)
                                                                                                                                        cggcATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGCTCTACCCGCACAAAATCGTACTGGCAGACGCCAAAATTGCCGATGCAGGCAAAATCCTTTCGCGTATGTGCTTCGaa           <  2:187035/145‑1 (MQ=255)
                                                                                                                                        cGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGCTCTACCCGCACAAAATCGTACTGGCAGACGCCAAAATTGCCGATGCAGGCAAAATCCTTTCGCGTATGTGCTTCGaa           >  2:3029705/1‑148 (MQ=255)
                                                                                                                                        cGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGCTCTACCCGCACAAAATCGTACTGGCAGACGCCAAAATTGCCGATGCAGGCAAAATCCTTTCGCGTATGTGCTTCGaa           >  2:2787736/1‑148 (MQ=255)
                                                                                                                                            aTTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGCTCTACCCGCACAAAATCGTACTGGCAGACGCCAAAATTGTCGATGCAGGCAAAATCCTTTCGCGTATGTGCTTCGAAGCCa       <  1:2511492/148‑1 (MQ=255)
                                                                                                                                            aTTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGCTCTACCCGCACAAAATCGTACTGGCAGACGCCAAAATTGCCGATGCAGGCAAAATCCTTTCGCGTATGTGCTTCGAAGCCa       <  2:1761096/148‑1 (MQ=255)
                                                                                                                                              tATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGCTCTACCCGCACAAAATCGTACTGGCAGACGCCAAAATTGCCGATGCAGGCAAAATCCTTTCGCGTATGTGCTTCGAAGCCAAc     >  1:27179/1‑148 (MQ=255)
                                                                                                                                               aTCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGCTCTACCCGCACAAAATCGTACTGGCAGACGCCAAAATTGCCGATGCAGGCAAAATCCTTTCGCGTATGTGCTTCGAAGCCAACg    <  1:3308392/148‑1 (MQ=255)
                                                                                                                                                tCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGCTCTACCCGCACAAAATCGTACTGGCAGACGCCAAAATTGCCGATGCAGGCAAAATCCTTTCGCGTATGTGCTTCGAAGCCAACGc   <  1:1743387/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
ACTGGATCTCATTGACCGCACCAACGCGGCAGCTTAAGAAGGAATTGAACATGTCATTACCGATGTTGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTCGCCTGATTGCCGAAGAAGTCGACATTATCGAAGTGGGCACCATTCTGTGCGTGGGCGAAGGCGTGCGTGCGGTTCGTGACCTGAAAGCGCTCTACCCGCACAAAATCGTACTGGCAGACGCCAAAATTGCCGATGCAGGCAAAATCCTTTCGCGTATGTGCTTCGAAGCCAACGCT  >  CP009273/4411953‑4412245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑