Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,412,786 G→T 100% E42* (GAA→TAA)  ulaE → L‑xylulose 5‑phosphate 3‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,412,7860GT97.4% 592.6 / 6.0 190E42* (GAA→TAA) ulaEL‑xylulose 5‑phosphate 3‑epimerase
Reads supporting (aligned to +/- strand):  ref base G (3/2);  new base T (98/87);  total (101/89)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 190 total aligned reads displayed.

ACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATG  >  CP009273/4412641‑4412927
                                                                                                                                                 |                                                                                                                                             
aCTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTaa                                                                                                                                             >  2:3437499/1‑148 (MQ=255)
aCTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTaa                                                                                                                                             <  2:552211/148‑1 (MQ=255)
 cTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTaaa                                                                                                                                            <  2:2599159/148‑1 (MQ=255)
   gtgGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACt                                                                                                                                          >  2:1229176/1‑148 (MQ=255)
    tgGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTg                                                                                                                                         <  1:2274353/148‑1 (MQ=255)
    tgGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTg                                                                                                                                         <  2:1754058/148‑1 (MQ=255)
     ggggCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGa                                                                                                                                        <  2:718187/148‑1 (MQ=255)
       ggCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACg                                                                                                                                      >  2:1707049/1‑148 (MQ=255)
        gCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGa                                                                                                                                     >  2:3237024/1‑148 (MQ=255)
         cTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGAt                                                                                                                                    >  1:2396292/1‑148 (MQ=255)
             ggAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGcc                                                                                                                                >  2:1397844/1‑148 (MQ=255)
             ggAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGcc                                                                                                                                >  2:1271563/1‑148 (MQ=255)
              gAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCt                                                                                                                               <  2:251563/148‑1 (MQ=255)
               aGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTg                                                                                                                              <  2:639912/148‑1 (MQ=255)
               aGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTg                                                                                                                              <  2:1065116/148‑1 (MQ=255)
                    atatGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTcgcg                                                                                                                         >  2:1509703/1‑148 (MQ=255)
                    atatGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTcgcg                                                                                                                         >  2:277985/1‑148 (MQ=255)
                     tatGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCgcgc                                                                                                                        >  1:1240624/1‑148 (MQ=255)
                       tgttgtCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCt                                                                                                                      <  1:864906/148‑1 (MQ=255)
                       tgttgtCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCt                                                                                                                      <  2:1982913/148‑1 (MQ=255)
                              caaacaaaTCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTgg                                                                                                               >  2:84121/1‑148 (MQ=255)
                                aacaaaTCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAg                                                                                                             >  1:1697006/1‑148 (MQ=255)
                                     aTCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCcgcg                                                                                                        >  1:2487120/1‑148 (MQ=255)
                                     aTCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCcgcg                                                                                                        <  1:3021924/148‑1 (MQ=255)
                                       cccGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGag                                                                                                      >  1:1439275/1‑148 (MQ=255)
                                       cccGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGag                                                                                                      >  2:539560/1‑148 (MQ=255)
                                       cccGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGag                                                                                                      >  2:3266498/1‑148 (MQ=255)
                                        ccGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGagc                                                                                                     <  2:318277/148‑1 (MQ=255)
                                          gCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGagcag                                                                                                   >  1:1279196/1‑148 (MQ=255)
                                          gCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGagcag                                                                                                   >  1:2697592/1‑148 (MQ=255)
                                             tGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGt                                                                                                >  1:1258990/1‑148 (MQ=255)
                                             tGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGt                                                                                                >  1:836571/1‑148 (MQ=255)
                                             tGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGt                                                                                                >  2:3025229/1‑148 (MQ=255)
                                             tGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGt                                                                                                <  1:2138332/148‑1 (MQ=255)
                                             tGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTCCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGt                                                                                                <  1:2098157/148‑1 (MQ=255)
                                                cATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTg                                                                                             >  1:1857783/1‑148 (MQ=255)
                                                cATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTg                                                                                             >  1:2793559/1‑148 (MQ=255)
                                                   cTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGgcg                                                                                          >  1:97713/1‑148 (MQ=255)
                                                   cTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGgcg                                                                                          >  1:2472221/1‑148 (MQ=255)
                                                      tGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTg                                                                                       <  1:3362426/148‑1 (MQ=255)
                                                          aaaGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCa                                                                                   >  2:300137/1‑148 (MQ=255)
                                                          aaaGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTAGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCa                                                                                   >  2:3233317/1‑148 (MQ=255)
                                                           aaGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAACACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCaa                                                                                  <  1:3474868/148‑1 (MQ=255)
                                                           aaGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCaa                                                                                  <  2:1279196/148‑1 (MQ=255)
                                                              cgcTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGc                                                                               >  2:1372307/1‑148 (MQ=255)
                                                              cgcTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGc                                                                               >  2:3140689/1‑148 (MQ=255)
                                                                  ccccGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGAtt                                                                           <  2:2246886/148‑1 (MQ=255)
                                                                   cccGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGAttg                                                                          <  1:1600662/148‑1 (MQ=255)
                                                                   cccGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGAttg                                                                          <  2:3514868/148‑1 (MQ=255)
                                                                    ccgccgGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGAttgt                                                                         <  2:1534317/148‑1 (MQ=255)
                                                                        cgGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGaa                                                                     <  1:2418422/148‑1 (MQ=255)
                                                                          gggAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAAc                                                                   >  1:1316481/1‑148 (MQ=255)
                                                                            gAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCg                                                                 >  1:1302539/1‑148 (MQ=255)
                                                                              gTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGgc                                                               <  1:1229176/148‑1 (MQ=255)
                                                                               tGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGgcg                                                              >  1:1496531/1‑148 (MQ=255)
                                                                                    gctggAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGgcgtgcgc                                                         <  1:91184/148‑1 (MQ=255)
                                                                                      tggAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGgcgtgcgcgt                                                       >  2:448633/1‑148 (MQ=255)
                                                                                        gAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGgcgtgcgcgtgc                                                     >  2:2464341/1‑148 (MQ=255)
                                                                                            gCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTc                                                 >  1:3137160/1‑148 (MQ=255)
                                                                                            gCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTc                                                 <  2:1540728/148‑1 (MQ=255)
                                                                                             ccTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAACCTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTcc                                                <  1:1819034/148‑1 (MQ=255)
                                                                                             ccTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTcc                                                >  1:612448/1‑148 (MQ=255)
                                                                                               tGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCAt                                              >  1:1378082/1‑148 (MQ=255)
                                                                                                gCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCAtg                                             >  1:676453/1‑148 (MQ=255)
                                                                                                  aaCTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCAtgtg                                           <  1:1477214/148‑1 (MQ=255)
                                                                                                    cTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGcc                                         >  1:1790148/1‑148 (MQ=255)
                                                                                                       gCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCttt                                      >  2:15944/1‑148 (MQ=255)
                                                                                                       gCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCttt                                      <  2:1624854/148‑1 (MQ=255)
                                                                                                        cAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTc                                     <  1:2847068/148‑1 (MQ=255)
                                                                                                          aaaaCGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTg                                   <  1:1271563/148‑1 (MQ=255)
                                                                                                          aaaaCGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTg                                   <  2:1358641/148‑1 (MQ=255)
                                                                                                           aaaCGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGc                                  >  1:290320/1‑148 (MQ=255)
                                                                                                            aaCGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCt                                 <  1:377029/148‑1 (MQ=255)
                                                                                                            aaCGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCt                                 >  2:3087157/1‑148 (MQ=255)
                                                                                                            aaCGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCt                                 <  1:1322296/148‑1 (MQ=255)
                                                                                                             aCGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTc                                <  2:869542/148‑1 (MQ=255)
                                                                                                             aCGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTc                                >  2:2616263/1‑148 (MQ=255)
                                                                                                                 tAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCAtcg                            >  1:590380/1‑148 (MQ=255)
                                                                                                                   ggCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCAtcgtc                          >  1:2211449/1‑148 (MQ=255)
                                                                                                                   ggCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCAtcgtc                          >  2:1875934/1‑148 (MQ=255)
                                                                                                                   ggCCTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCCATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCAtcgtc                          >  2:3378267/1‑148 (MQ=255)
                                                                                                                    gCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCAtcgtcg                         >  2:1992253/1‑148 (MQ=255)
                                                                                                                     ctgcGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATcgtcgt                        <  2:3129015/145‑1 (MQ=255)
                                                                                                                     cTTCGCTTTTGTCGAAATGTCGGTAGATTAAACGGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATcgtcgt                        <  2:1814543/148‑1 (MQ=255)
                                                                                                                      ttCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGtt                       <  2:387789/148‑1 (MQ=255)
                                                                                                                        cGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTc                     <  2:1353176/148‑1 (MQ=255)
                                                                                                                        cGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTc                     >  1:1876769/1‑148 (MQ=255)
                                                                                                                           ttttGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTcccc                  >  1:2107641/1‑147 (MQ=255)
                                                                                                                           ttttGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTGTACCg                  >  1:2107758/1‑148 (MQ=255)
                                                                                                                             ttGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGACACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCt                <  1:3326505/148‑1 (MQ=255)
                                                                                                                              tGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTg               <  2:2174912/148‑1 (MQ=255)
                                                                                                                                 cGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGc            >  1:3402975/1‑148 (MQ=255)
                                                                                                                                    aaTGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGt         <  1:2914361/148‑1 (MQ=255)
                                                                                                                                     aTGTCTGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTg        >  2:1166485/1‑148 (MQ=255)
                                                                                                                                     aTGTCTGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTg        >  2:3452147/1‑148 (MQ=255)
                                                                                                                                     aTGTCGGTAGATTAAACTGACGATCGCCTGTCGTGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTg        >  1:1733873/1‑148 (MQ=255)
                                                                                                                                     aTGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTg        >  1:3399985/1‑148 (MQ=255)
                                                                                                                                     aTGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCGGGGCAGTg        >  1:2561961/1‑148 (MQ=255)
                                                                                                                                        tCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAg     <  1:2502361/148‑1 (MQ=255)
                                                                                                                                        tCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAg     <  1:3266498/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                             
ACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATG  >  CP009273/4412641‑4412927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑