Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,417,122 G→T 100% R40S (CGT→AGT)  yjfZ ← uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,417,1220GT97.8% 692.2 / 5.6 232R40S (CGT→AGT) yjfZuncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (3/2);  new base T (100/126);  total (104/128)
Fisher's exact test for biased strand distribution p-value = 6.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.36e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 232 total aligned reads displayed.

CATCCAAAAGAATTATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACGATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAATGTTTTT  >  CP009273/4416977‑4417265
                                                                                                                                                 |                                                                                                                                               
catccaAAAGAATTATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTAt                                                                                                                                               <  1:2464265/148‑1 (MQ=255)
catccaAAAGAATTATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTAt                                                                                                                                               >  2:1607888/1‑148 (MQ=255)
 atccaAAAGAATTATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGGTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTAtt                                                                                                                                              >  2:2635267/1‑148 (MQ=255)
  tccaAAAGAATTATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTAttt                                                                                                                                             >  2:2383494/1‑148 (MQ=255)
  tccaAAAGAATTATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTAAAAAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTAttt                                                                                                                                             >  2:302310/1‑148 (MQ=255)
    caAAAGAATTATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGa                                                                                                                                           <  2:656720/148‑1 (MQ=255)
     aaaaGAATTATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTACACTATTTGaa                                                                                                                                          >  1:1655817/1‑148 (MQ=255)
           aTTATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAAt                                                                                                                                    >  2:2907023/1‑148 (MQ=255)
            ttATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATg                                                                                                                                   >  1:3069399/1‑148 (MQ=255)
              aTCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTa                                                                                                                                 >  2:2105061/1‑148 (MQ=255)
                  ccATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAgcgc                                                                                                                             >  1:1500314/1‑148 (MQ=255)
                    attcattGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCgcgt                                                                                                                           >  1:2369520/1‑148 (MQ=255)
                     ttcattGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCgcgtg                                                                                                                          >  2:2865159/1‑148 (MQ=255)
                         ttGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTa                                                                                                                      <  1:3480503/148‑1 (MQ=255)
                             gATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATtga                                                                                                                  >  2:1724000/1‑148 (MQ=255)
                              aTTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATtgat                                                                                                                 >  2:271809/1‑148 (MQ=255)
                                tCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATTGTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTAAGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATTTATCGCGTGCGGATtgatga                                                                                                               >  1:389938/1‑148 (MQ=255)
                                 ccATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGaa                                                                                                              <  2:909626/148‑1 (MQ=255)
                                  cATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGaaa                                                                                                             >  2:2891798/1‑148 (MQ=255)
                                    tCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAaatt                                                                                                           <  1:1785280/148‑1 (MQ=255)
                                     cGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAaattt                                                                                                          <  1:643519/148‑1 (MQ=255)
                                     cGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAaattt                                                                                                          >  1:272349/1‑148 (MQ=255)
                                       tAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAaatttta                                                                                                        >  1:1769931/1‑148 (MQ=255)
                                       tAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACGATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAaatttta                                                                                                        <  2:88/148‑1 (MQ=255)
                                         aGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTATCGCGTGCGTATTGTTGAaattttaat                                                                                                      >  1:2294030/1‑148 (MQ=255)
                                         aGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAaattttaat                                                                                                      <  1:394381/148‑1 (MQ=255)
                                          gCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAaattttaatt                                                                                                     <  2:3253002/148‑1 (MQ=255)
                                          gCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAaattttaatt                                                                                                     <  1:544808/148‑1 (MQ=255)
                                          gCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAaattttaatt                                                                                                     >  2:378271/1‑148 (MQ=255)
                                             ttACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTAACTCTGCAATCGCAATTAAGATCACTTCAAATATTTTAAAGTAATGTAGAGCGTGCGTATTGATGAAattttaatttta                                                                                                  >  1:2614668/1‑148 (MQ=255)
                                              tACACCTCGTCGCGAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAATTAATGTAGCGCGTGCGTATTGATGAAattttaattttaa                                                                                                 >  1:2653068/1‑147 (MQ=255)
                                                  ccTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGt                                                                                             <  1:2691402/148‑1 (MQ=255)
                                                  ccTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGt                                                                                             <  1:3441287/148‑1 (MQ=255)
                                                  ccTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGt                                                                                             <  1:404066/148‑1 (MQ=255)
                                                    tcgtcgCTAACTTATTGAATGCTTGCCAGTCTGATAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGttt                                                                                           <  2:2025327/148‑1 (MQ=255)
                                                    tcgtcgCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAACTTTAATTTTACTGGttt                                                                                           >  1:551060/1‑148 (MQ=255)
                                                     cgtcgCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTg                                                                                          <  1:1867118/148‑1 (MQ=255)
                                                      gtcgCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGt                                                                                         <  2:2430587/148‑1 (MQ=255)
                                                         gCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCgg                                                                                      <  1:3143691/148‑1 (MQ=255)
                                                          cTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGt                                                                                     <  2:3472551/148‑1 (MQ=255)
                                                          cTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGt                                                                                     >  2:3537094/1‑148 (MQ=255)
                                                          cTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGt                                                                                     <  2:2483852/148‑1 (MQ=255)
                                                                tATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGa                                                                               >  1:94336/1‑148 (MQ=255)
                                                                tATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGa                                                                               >  2:3323112/1‑148 (MQ=255)
                                                                     aaTGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAAc                                                                          >  2:2461097/1‑148 (MQ=255)
                                                                      aTGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACg                                                                         <  2:1117366/148‑1 (MQ=255)
                                                                         cTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACgaga                                                                      <  2:2502739/148‑1 (MQ=255)
                                                                         cTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACgaga                                                                      <  1:3258487/148‑1 (MQ=255)
                                                                         cTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACgaga                                                                      <  2:717992/148‑1 (MQ=255)
                                                                             ccAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATaaa                                                                  <  2:3053875/148‑1 (MQ=255)
                                                                              cAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAat                                                                 <  2:757737/148‑1 (MQ=255)
                                                                               aGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAata                                                                <  1:3007667/148‑1 (MQ=255)
                                                                               aGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAata                                                                >  2:2275614/1‑148 (MQ=255)
                                                                               aGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAata                                                                >  1:1598532/1‑148 (MQ=255)
                                                                                gTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAatat                                                               <  2:253843/148‑1 (MQ=255)
                                                                                  cTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGc                                                             >  2:413563/1‑148 (MQ=255)
                                                                                   tGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCa                                                            >  2:1258043/1‑148 (MQ=255)
                                                                                   tGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCa                                                            <  2:2238479/148‑1 (MQ=255)
                                                                                   tGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCa                                                            >  1:784954/1‑148 (MQ=255)
                                                                                     agagaTCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACGATTTGAAAGTAAGGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAgg                                                          >  1:2097429/1‑148 (MQ=255)
                                                                                      gagaTCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGa                                                         <  1:2892011/148‑1 (MQ=255)
                                                                                       agaTCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACGATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGaa                                                        >  2:180503/1‑148 (MQ=255)
                                                                                           cTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGa                                                    >  1:3160444/1‑148 (MQ=255)
                                                                                           cTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGa                                                    <  1:1625539/148‑1 (MQ=255)
                                                                                                ttGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGaataaa                                               <  1:1332660/148‑1 (MQ=255)
                                                                                                 tgtgGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGaataaat                                              <  1:901357/148‑1 (MQ=255)
                                                                                                  gtgGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGaataaata                                             <  1:1217543/148‑1 (MQ=255)
                                                                                                  gtgGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGaataaata                                             <  1:4982/148‑1 (MQ=255)
                                                                                                   tgGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAg                                            <  2:1471365/148‑1 (MQ=255)
                                                                                                   tgGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAg                                            <  1:3397586/148‑1 (MQ=255)
                                                                                                     gATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTc                                          <  1:2490731/148‑1 (MQ=255)
                                                                                                     gATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTc                                          <  1:2635267/148‑1 (MQ=255)
                                                                                                       tctcAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGt                                        >  1:1200486/1‑148 (MQ=255)
                                                                                                            aCAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTCGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTa                                   <  1:2788358/148‑1 (MQ=255)
                                                                                                            aCAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTa                                   <  1:1015469/148‑1 (MQ=255)
                                                                                                             cAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTaa                                  <  2:2975127/148‑1 (MQ=255)
                                                                                                              aGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAAt                                 <  2:3119581/148‑1 (MQ=255)
                                                                                                               gTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATg                                <  2:817166/148‑1 (MQ=255)
                                                                                                               gTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATg                                <  1:2778953/148‑1 (MQ=255)
                                                                                                                 aCGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTc                              >  2:1359012/1‑148 (MQ=255)
                                                                                                                 aCGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTc                              <  1:684205/148‑1 (MQ=255)
                                                                                                                    tCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCAtt                           >  2:2314531/1‑148 (MQ=255)
                                                                                                                     cTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTg                          >  1:2577839/1‑148 (MQ=255)
                                                                                                                      tGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGt                         >  2:528002/1‑148 (MQ=255)
                                                                                                                        ccATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTaa                       <  2:312711/148‑1 (MQ=255)
                                                                                                                        ccATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTaa                       <  2:2086157/148‑1 (MQ=255)
                                                                                                                          aTCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATg                     >  2:1181897/1‑148 (MQ=255)
                                                                                                                          aTCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATg                     <  1:302310/148‑1 (MQ=255)
                                                                                                                          aTCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATg                     >  2:2216895/1‑148 (MQ=255)
                                                                                                                            cGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGtt                   >  2:1332016/1‑148 (MQ=255)
                                                                                                                             gCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTa                  >  1:2940456/1‑148 (MQ=255)
                                                                                                                              cAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGAAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTAc                 >  1:2200434/1‑148 (MQ=255)
                                                                                                                                aTTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCt               >  1:1959921/1‑148 (MQ=255)
                                                                                                                                  tATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGt             >  1:932553/1‑148 (MQ=255)
                                                                                                                                       tctcTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAAt        <  1:3198961/148‑1 (MQ=255)
                                                                                                                                       tctcTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAAt        >  2:2574398/1‑148 (MQ=255)
                                                                                                                                          ctTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAATGtt     <  2:1500314/148‑1 (MQ=255)
                                                                                                                                          ctTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAATGtt     <  1:2140707/148‑1 (MQ=255)
                                                                                                                                           ttCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAATGttt    >  1:340583/1‑148 (MQ=255)
                                                                                                                                            tCCACTATAAGAAACAAATGTATCGAGTGCGTATTGATGAAATTATAATAATAATTGTATGTCGTTGTACGAAAAACGAGATAAATATACACGAAAAGAATAAAAAGACTTTGGTAATGTAATATAAATGTTAACTCTTAAATGattt   >  2:1621288/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CATCCAAAAGAATTATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACGATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAATGTTTTT  >  CP009273/4416977‑4417265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑