Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,456,233 G→T 100% I277I (ATC→ATA)  treR ← trehalose 6‑phosphate‑inducible trehalose regulon transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,456,2330GT98.1% 636.6 / 3.3 206I277I (ATC→ATA) treRtrehalose 6‑phosphate‑inducible trehalose regulon transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base G (1/3);  new base T (76/126);  total (77/129)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 206 total aligned reads displayed.

AGATCACAATTTATCGTTCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTA  >  CP009273/4456088‑4456377
                                                                                                                                                 |                                                                                                                                                
 gATCACAATTTATCGTTCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTAtc                                                                                                                                               >  2:2076777/1‑148 (MQ=255)
 gATCACAATTTATCGTTCAGGAAACGATCAGGACAGGGTGGCGGGGATGAGGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTAtc                                                                                                                                               >  1:534897/1‑148 (MQ=255)
  aTCACAATTTATCGTTCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGGTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGTCGTAACCGGGATATACGGTTACTAtct                                                                                                                                              <  2:1173632/148‑1 (MQ=255)
   tCACAATTTATCGTTCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTAtctc                                                                                                                                             >  1:156154/1‑148 (MQ=255)
      caATTTATCGTTCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCgg                                                                                                                                          >  2:657444/1‑148 (MQ=255)
       aaTTTATCGTTCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGa                                                                                                                                         >  1:1053726/1‑148 (MQ=255)
       aaTTTATCGTTCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGa                                                                                                                                         >  1:2826887/1‑148 (MQ=255)
        aTTTATCGTTCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGAt                                                                                                                                        >  2:2574106/1‑148 (MQ=255)
        aTTTATCGTTCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGAt                                                                                                                                        >  2:178651/1‑148 (MQ=255)
        aTTTATCGTTCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGAt                                                                                                                                        >  2:792713/1‑148 (MQ=255)
         tttATCGTTCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATg                                                                                                                                       >  2:388946/1‑148 (MQ=255)
          ttATCGTTCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATgg                                                                                                                                      <  2:853937/148‑1 (MQ=255)
          ttATCGTTCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATgg                                                                                                                                      >  1:78303/1‑148 (MQ=255)
           tATCGTTCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATgga                                                                                                                                     <  1:2537665/148‑1 (MQ=255)
            aTCGTTCAGGAAACGATCCGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATggag                                                                                                                                    <  1:2172029/148‑1 (MQ=255)
            aTCGTTCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATggag                                                                                                                                    <  2:3223408/148‑1 (MQ=255)
            aTCGTTCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATggag                                                                                                                                    <  2:1457809/148‑1 (MQ=255)
            aTCGTTCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATggag                                                                                                                                    >  1:38065/1‑148 (MQ=255)
                ttCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAAt                                                                                                                                <  2:2246791/148‑1 (MQ=255)
                 tCAGGAGACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGaa                                                                                                                                 >  2:2553679/1‑146 (MQ=255)
                 tCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGaa                                                                                                                                 <  1:1842262/146‑1 (MQ=255)
                 tCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAAtt                                                                                                                               <  1:1887728/148‑1 (MQ=255)
                 tCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAAtt                                                                                                                               >  1:1627006/1‑148 (MQ=255)
                  cAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAAttt                                                                                                                              <  2:638382/148‑1 (MQ=255)
                   aGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTc                                                                                                                             <  1:1760372/148‑1 (MQ=255)
                    ggAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCa                                                                                                                            >  2:1848904/1‑148 (MQ=255)
                    ggAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCa                                                                                                                            >  1:2818477/1‑148 (MQ=255)
                     gAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCAt                                                                                                                           <  1:2144286/148‑1 (MQ=255)
                     gAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGGTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCAt                                                                                                                           <  1:13674/148‑1 (MQ=255)
                      aaaCGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCAtt                                                                                                                          <  1:1125812/148‑1 (MQ=255)
                       aaCGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTa                                                                                                                         >  2:1675388/1‑148 (MQ=255)
                           aTCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGCTCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACgg                                                                                                                     >  2:3437166/1‑148 (MQ=255)
                            tCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGc                                                                                                                    <  1:1620949/148‑1 (MQ=255)
                              aGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCGGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGt                                                                                                                  <  2:376067/148‑1 (MQ=255)
                               ggACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCTGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTa                                                                                                                 <  1:1797316/148‑1 (MQ=255)
                                gACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTAt                                                                                                                >  1:2973056/1‑148 (MQ=255)
                                gACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTAt                                                                                                                >  1:3268823/1‑148 (MQ=255)
                                     ggTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCg                                                                                                           >  2:1247032/1‑148 (MQ=255)
                                            gggATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCg                                                                                                    >  1:654587/1‑148 (MQ=255)
                                                tgatgatTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAg                                                                                                <  2:2139360/148‑1 (MQ=255)
                                                 gatgatTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGt                                                                                               >  1:399317/1‑148 (MQ=255)
                                                       ttgttgCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAg                                                                                         >  1:2585468/1‑148 (MQ=255)
                                                       ttgttgCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAg                                                                                         >  1:1830705/1‑148 (MQ=255)
                                                       ttgttgCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAg                                                                                         >  1:19057/1‑148 (MQ=255)
                                                        tgttgCGGTTCGCTGCGCCCGGGTACCGGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAgg                                                                                        <  2:2460136/148‑1 (MQ=255)
                                                          ttgCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGgtg                                                                                      <  1:2605292/148‑1 (MQ=255)
                                                                  cGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCg                                                                              <  2:2981010/148‑1 (MQ=255)
                                                                      gcgcCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGc                                                                          <  2:3288832/148‑1 (MQ=255)
                                                                      gcgcCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGc                                                                          >  2:210929/1‑148 (MQ=255)
                                                                       cgcCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGct                                                                         <  2:3174599/148‑1 (MQ=255)
                                                                       cgcCCGGTGACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGct                                                                         <  2:2764238/148‑1 (MQ=255)
                                                                       cgcCCGGGTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGct                                                                         <  2:236073/148‑1 (MQ=255)
                                                                       cgcCCGGGGACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGct                                                                         <  2:1658014/148‑1 (MQ=255)
                                                                         cccGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGctct                                                                       >  1:1464465/1‑148 (MQ=255)
                                                                           ctgTTACCTGCGAGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGTATATACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGTTGTCGATGCGTTGCTCTTg                                                                     <  2:30815/146‑1 (MQ=255)
                                                                           cGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTg                                                                     <  2:91459/148‑1 (MQ=255)
                                                                            ggTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGc                                                                    >  1:2661398/1‑148 (MQ=255)
                                                                              ttACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAg                                                                  <  2:3318834/148‑1 (MQ=255)
                                                                                aCCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCCTTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGt                                                                <  1:3344639/148‑1 (MQ=255)
                                                                                 ccTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTa                                                               <  1:3543112/148‑1 (MQ=255)
                                                                                  cTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTAt                                                              >  1:2927430/1‑148 (MQ=255)
                                                                                  cTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTAt                                                              <  1:3247195/148‑1 (MQ=255)
                                                                                  cTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTAt                                                              <  1:3437935/148‑1 (MQ=255)
                                                                                   tGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTAtt                                                             <  2:2826887/148‑1 (MQ=255)
                                                                                   tGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTAtt                                                             <  1:958026/148‑1 (MQ=255)
                                                                                    gcgcGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTAttt                                                            <  2:1230108/148‑1 (MQ=255)
                                                                                    gcgcGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTAttt                                                            <  2:1229907/148‑1 (MQ=255)
                                                                                    gcgcGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTAttt                                                            >  2:2352995/1‑148 (MQ=255)
                                                                                     cgcgATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACTGTTACTATCTCCGGATGGAGGAATTACATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTa                                                           >  1:2464354/1‑148 (MQ=255)
                                                                                      gcgATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTAc                                                          <  2:328013/148‑1 (MQ=255)
                                                                                      gcgATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTAc                                                          <  2:328767/148‑1 (MQ=255)
                                                                                       cgATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACt                                                         <  2:1564846/148‑1 (MQ=255)
                                                                                        gATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACtt                                                        >  2:2958116/1‑148 (MQ=255)
                                                                                           cAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTgcg                                                     <  2:1114341/148‑1 (MQ=255)
                                                                                           cAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTgcg                                                     <  1:3507669/148‑1 (MQ=255)
                                                                                              cTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCa                                                  <  2:151832/148‑1 (MQ=255)
                                                                                                  cAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTg                                              <  2:1384471/148‑1 (MQ=255)
                                                                                                  cAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTg                                              >  1:2363158/1‑148 (MQ=255)
                                                                                                   aaGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGc                                             <  2:2721726/148‑1 (MQ=255)
                                                                                                   aaGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGc                                             <  2:2860089/148‑1 (MQ=255)
                                                                                                   aaGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGc                                             <  2:3118989/148‑1 (MQ=255)
                                                                                                   aaGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGc                                             <  2:944483/148‑1 (MQ=255)
                                                                                                          cTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCgtg                                      <  1:670546/148‑1 (MQ=255)
                                                                                                             gCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGAGGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCg                                   >  1:1217787/1‑148 (MQ=255)
                                                                                                                  cAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTgc                              >  1:562777/1‑148 (MQ=255)
                                                                                                                        cGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAg                        <  2:1928399/148‑1 (MQ=255)
                                                                                                                        cGGCGTAACCGGGATCTACGGTTACTATCTCCGGAGGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAg                        <  2:1075778/148‑1 (MQ=255)
                                                                                                                         ggCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGt                       >  2:506385/1‑148 (MQ=255)
                                                                                                                           cGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTaa                     <  1:2883956/148‑1 (MQ=255)
                                                                                                                            gTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAg                    <  2:1464465/148‑1 (MQ=255)
                                                                                                                               aCCGGGATCTACGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCg                 <  2:3025949/148‑1 (MQ=255)
                                                                                                                                 cGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGt               <  1:3512383/148‑1 (MQ=255)
                                                                                                                                  gggATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTa              >  1:1098276/1‑148 (MQ=255)
                                                                                                                                    gATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGt            >  1:2003731/1‑148 (MQ=255)
                                                                                                                                      tCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGttt          >  1:2057496/1‑148 (MQ=255)
                                                                                                                                      tCTACGGGTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGttt          <  2:1801576/148‑1 (MQ=255)
                                                                                                                                       cTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTc         <  2:267101/148‑1 (MQ=255)
                                                                                                                                         aCGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAg       >  2:250404/1‑148 (MQ=255)
                                                                                                                                            gTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCg    >  1:2752135/1‑148 (MQ=255)
                                                                                                                                              tACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTa  <  2:963760/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
AGATCACAATTTATCGTTCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTA  >  CP009273/4456088‑4456377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑