Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,458,609 G→T 100% D390Y (GAT→TAT)  mgtA → magnesium transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,458,6090GT100.0% 712.7 / NA 213D390Y (GAT→TAT) mgtAmagnesium transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (105/108);  total (105/108)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 217 total aligned reads displayed.

GGCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGC  >  CP009273/4458464‑4458753
                                                                                                                                                 |                                                                                                                                                
ggCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTat                                                                                                                                                <  2:790557/148‑1 (MQ=255)
  cgcgcgGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTatat                                                                                                                                              >  2:463943/1‑148 (MQ=255)
  cgcgcgGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTatat                                                                                                                                              <  2:1969249/148‑1 (MQ=255)
    cgcgGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATAtct                                                                                                                                            <  1:2287320/148‑1 (MQ=255)
        gcgcAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCgg                                                                                                                                        >  2:2895838/1‑148 (MQ=255)
         cgcAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGt                                                                                                                                       <  1:2929341/148‑1 (MQ=255)
             gTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTaaaa                                                                                                                                   <  1:1468798/148‑1 (MQ=255)
              tAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAAc                                                                                                                                  <  2:516899/148‑1 (MQ=255)
              tAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAAc                                                                                                                                  <  2:2887145/148‑1 (MQ=255)
               aaaGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAAcc                                                                                                                                 >  1:1508071/1‑148 (MQ=255)
                 aGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAg                                                                                                                               <  2:1061278/148‑1 (MQ=255)
                 aGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAg                                                                                                                               >  1:2454237/1‑148 (MQ=255)
                  gCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGc                                                                                                                              >  2:2447226/1‑148 (MQ=255)
                        gAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAAcgc                                                                                                                        <  2:3065965/148‑1 (MQ=255)
                        gAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAAcgc                                                                                                                        <  2:1254680/148‑1 (MQ=255)
                        gAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAAcgc                                                                                                                        <  2:1818235/148‑1 (MQ=255)
                        gAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAAcgc                                                                                                                        <  2:2957326/148‑1 (MQ=255)
                          aaCAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGt                                                                                                                      >  2:2665796/1‑148 (MQ=255)
                           aCAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGtg                                                                                                                     <  2:2621929/148‑1 (MQ=255)
                           aCAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGtg                                                                                                                     <  1:1362804/148‑1 (MQ=255)
                            cAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGtgc                                                                                                                    <  2:168037/148‑1 (MQ=255)
                            cAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGtgc                                                                                                                    <  2:168019/148‑1 (MQ=255)
                            cAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGtgc                                                                                                                    <  2:167077/148‑1 (MQ=255)
                               aaaGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGtgctgc                                                                                                                 >  1:1926727/1‑148 (MQ=255)
                                aaGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATCTTCTGTGCACTGATACAACCGGCAACCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCa                                                                                                                <  1:2661700/148‑1 (MQ=255)
                                 aGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCAt                                                                                                               >  1:735431/1‑148 (MQ=255)
                                   tGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAg                                                                                                             >  2:2513450/1‑148 (MQ=255)
                                   tGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAg                                                                                                             >  2:3424807/1‑148 (MQ=255)
                                   tGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAg                                                                                                             <  2:973202/148‑1 (MQ=255)
                                   tGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAg                                                                                                             <  1:658907/148‑1 (MQ=255)
                                     aTCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAgcg                                                                                                           >  2:3013450/1‑148 (MQ=255)
                                       cGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGcgcg                                                                                                         <  2:2750004/148‑1 (MQ=255)
                                          cAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTgg                                                                                                      >  2:3067495/1‑148 (MQ=255)
                                           aaaCATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGt                                                                                                     <  2:2089549/148‑1 (MQ=255)
                                            aaCATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGtt                                                                                                    >  2:3036039/1‑148 (MQ=255)
                                              cATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGa                                                                                                  <  1:422199/148‑1 (MQ=255)
                                               aTCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGaa                                                                                                 >  2:3523741/1‑148 (MQ=255)
                                                tCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAAc                                                                                                <  2:2852178/148‑1 (MQ=255)
                                                 cTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACa                                                                                               <  2:2385320/148‑1 (MQ=255)
                                                    gATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGta                                                                                            <  1:2772860/148‑2 (MQ=255)
                                                     aTGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCa                                                                                           >  1:1104219/1‑148 (MQ=255)
                                                     aTGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCa                                                                                           >  1:3338343/1‑148 (MQ=255)
                                                         tATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTAt                                                                                       >  2:3454092/1‑148 (MQ=255)
                                                          aTTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATc                                                                                      <  2:2359316/148‑1 (MQ=255)
                                                          aTTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATc                                                                                      >  2:1400254/1‑148 (MQ=255)
                                                            tCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAg                                                                                    >  2:2184290/1‑148 (MQ=255)
                                                            tCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAg                                                                                    >  1:488365/1‑148 (MQ=255)
                                                            tCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAg                                                                                    <  1:2434294/148‑1 (MQ=255)
                                                            tCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAg                                                                                    >  1:1730378/1‑148 (MQ=255)
                                                             cAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGa                                                                                   <  1:2338394/148‑1 (MQ=255)
                                                             cAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGa                                                                                   <  1:2705997/148‑1 (MQ=255)
                                                                  cTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGa                                                                              <  2:2834472/148‑1 (MQ=255)
                                                                   tttGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGAc                                                                             >  1:504643/1‑148 (MQ=255)
                                                                   tttGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGAc                                                                             <  2:189313/148‑1 (MQ=255)
                                                                    ttGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACt                                                                            <  2:1457365/148‑1 (MQ=255)
                                                                       gcgcAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTaa                                                                         >  1:3551111/1‑148 (MQ=255)
                                                                          cAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTaaaaa                                                                      >  2:1623556/1‑148 (MQ=255)
                                                                            aTGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAAcc                                                                    <  1:529768/148‑1 (MQ=255)
                                                                            aTGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAAcc                                                                    >  2:788597/1‑148 (MQ=255)
                                                                            aTGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAAcc                                                                    >  2:2789449/1‑148 (MQ=255)
                                                                               gATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGc                                                                 >  2:2544170/1‑148 (MQ=255)
                                                                                atatTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCt                                                                <  2:2787661/148‑1 (MQ=255)
                                                                                  cttCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCg                                                              <  1:2605415/147‑1 (MQ=255)
                                                                                   ttCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGa                                                             <  2:2200582/148‑1 (MQ=255)
                                                                                    tCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGAt                                                            >  2:726415/1‑148 (MQ=255)
                                                                                     cTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATa                                                           <  2:949301/148‑1 (MQ=255)
                                                                                        tgCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAg                                                        >  1:2824697/1‑148 (MQ=255)
                                                                                        tgCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAg                                                        >  1:1030525/1‑148 (MQ=255)
                                                                                         gCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAATACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGc                                                       >  1:2150904/1‑148 (MQ=255)
                                                                                          cACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCg                                                      >  2:1411013/1‑148 (MQ=255)
                                                                                           aCTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCgg                                                     <  2:1090030/148‑1 (MQ=255)
                                                                                              gATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGc                                                  >  1:619135/1‑148 (MQ=255)
                                                                                              gATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGc                                                  >  1:627216/1‑148 (MQ=255)
                                                                                               aTAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCt                                                 >  1:3150115/1‑148 (MQ=255)
                                                                                                 aaaaCCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCg                                               >  1:2779583/1‑148 (MQ=255)
                                                                                                   aaCCGTCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGaa                                             >  1:1772823/1‑148 (MQ=255)
                                                                                                   aaCCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGaa                                             <  1:1623556/148‑1 (MQ=255)
                                                                                                   aaCCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGaa                                             >  1:3379589/1‑148 (MQ=255)
                                                                                                      cGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGt                                          <  2:873680/148‑1 (MQ=255)
                                                                                                                   cAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGt                             >  2:3257003/1‑148 (MQ=255)
                                                                                                                   cAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGt                             <  2:1243671/148‑1 (MQ=255)
                                                                                                                    aGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTTCATTGCGCGTGGTTGAACAGTCATTATAAGACCGGACTTAAAAACCTGCTCGATACATCGTTGCTCGAAGGTACGTATGAAGAGTc                            <  2:1696646/148‑1 (MQ=255)
                                                                                                                    aGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTc                            <  1:143104/148‑1 (MQ=255)
                                                                                                                     ggATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCa                           >  2:932701/1‑148 (MQ=255)
                                                                                                                      gATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAg                          <  1:1830077/148‑1 (MQ=255)
                                                                                                                       aTAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAgc                         >  2:1066197/1‑148 (MQ=255)
                                                                                                                        tAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAgcg                        >  2:2265526/1‑148 (MQ=255)
                                                                                                                           aaTTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAgcgcgc                     <  1:3013450/148‑1 (MQ=255)
                                                                                                                            aTTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGcgct                    <  1:3289538/148‑1 (MQ=255)
                                                                                                                                     gagaATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCa           >  1:1308745/1‑148 (MQ=255)
                                                                                                                                      agaATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAg          <  2:1563687/148‑1 (MQ=255)
                                                                                                                                      agaATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAg          <  2:3482468/148‑1 (MQ=255)
                                                                                                                                       gaATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGt         >  1:2209859/1‑148 (MQ=255)
                                                                                                                                       gaATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGt         <  1:3454092/148‑1 (MQ=255)
                                                                                                                                       gaATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGt         >  2:1036959/1‑148 (MQ=255)
                                                                                                                                          tCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGt      <  2:1730378/148‑1 (MQ=255)
                                                                                                                                            aTACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTg    <  1:3159674/148‑1 (MQ=255)
                                                                                                                                            aTACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTg    >  2:50244/1‑148 (MQ=255)
                                                                                                                                            aTACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTg    <  2:2481215/148‑1 (MQ=255)
                                                                                                                                              aCCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGc  <  1:790193/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GGCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGC  >  CP009273/4458464‑4458753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑