Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,481,076 T→G 42.4% I423L (ATT→CTT)  idnT ← L‑idonate and D‑gluconate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,481,0760TG42.4% 16.1 / 271.2 184I423L (ATT→CTT) idnTL‑idonate and D‑gluconate transporter
Reads supporting (aligned to +/- strand):  ref base T (60/46);  new base G (45/33);  total (105/79)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

Only 100 of 196 total aligned reads displayed.

CCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGCCAGTACCATTAAAGCGGGATCGGC  >  CP009273/4480929‑4481223
                                                                                                                                                   |                                                                                                                                                   
ccTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAAt                                                                                                                                                     >  2:2213743/1‑148 (MQ=255)
ccTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAAt                                                                                                                                                     >  2:1343048/1‑148 (MQ=255)
ccTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAAt                                                                                                                                                     <  1:2547127/148‑1 (MQ=255)
ccTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTGATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAAt                                                                                                                                                     >  2:2377701/1‑148 (MQ=255)
   gTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAaagaag                                                                                                                                                  >  1:1056810/1‑148 (MQ=255)
   gTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAaagaag                                                                                                                                                  >  2:2496988/1‑148 (MQ=255)
   gTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAaagaag                                                                                                                                                  >  1:1055769/1‑148 (MQ=255)
     aaaGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGACATAAggg                                                                                                                                                >  2:858927/1‑148 (MQ=255)
      aaGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGt                                                                                                                                               >  2:3435781/1‑148 (MQ=255)
      aaGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGt                                                                                                                                               >  1:2205886/1‑148 (MQ=255)
      aaGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAAGAAGGGt                                                                                                                                               <  2:668408/148‑1 (MQ=255)
       aGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGtt                                                                                                                                              <  1:1247547/148‑1 (MQ=255)
       aGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGtt                                                                                                                                              <  1:1244158/148‑1 (MQ=255)
        gAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAAGAAGGGttt                                                                                                                                             <  1:3038427/148‑1 (MQ=255)
                   aTTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAAGAAGGGTTTCCATCACCGTc                                                                                                                                  <  2:2239437/148‑1 (MQ=255)
                    ttGCGCATAACTTCTCCCAACGTCGGGGGAATCTTAAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAAGAAGGGTTTCCATCACCGTcc                                                                                                                                 >  1:568220/1‑148 (MQ=255)
                     tGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAAGAAGGGTTTCCATCACCGTCCa                                                                                                                                >  2:1623795/1‑148 (MQ=255)
                        gcATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAAGAAGGGTTTCCATCACCGTCCAGGt                                                                                                                             <  2:3031703/148‑1 (MQ=255)
                         cATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGGTTCCATCACCGTCCAGGTa                                                                                                                            >  2:2286964/1‑148 (MQ=255)
                               ttCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAAGAAGGGTTTCCATCACCGTCCAGGTACGCAAc                                                                                                                      >  1:2411485/1‑148 (MQ=255)
                                tctcCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACg                                                                                                                     <  1:267626/148‑1 (MQ=255)
                                tctcCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACg                                                                                                                     <  2:2271229/148‑1 (MQ=255)
                                tctcCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACg                                                                                                                     >  2:3204322/1‑148 (MQ=255)
                                 ctcCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGt                                                                                                                    <  2:604168/148‑1 (MQ=255)
                                   cccAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGttt                                                                                                                  <  2:320941/148‑1 (MQ=255)
                                   cccAACGTCGGGGGAAACTACAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGttt                                                                                                                  <  2:1936260/148‑1 (MQ=255)
                                       aCGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCAcc                                                                                                              >  2:1845072/1‑148 (MQ=255)
                                        cGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAAGAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCa                                                                                                             >  1:171013/1‑148 (MQ=255)
                                           cGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAAGAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAAcc                                                                                                          <  2:1031365/148‑1 (MQ=255)
                                           cGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAAGAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAAcc                                                                                                          <  2:1031353/148‑1 (MQ=255)
                                             ggggAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGt                                                                                                        <  1:3167775/148‑1 (MQ=255)
                                             ggggAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAAGAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGt                                                                                                        >  1:2506053/1‑148 (MQ=255)
                                               ggAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAAGAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCa                                                                                                      <  1:2213066/148‑1 (MQ=255)
                                                gAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGACAGAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAg                                                                                                     <  2:476621/148‑1 (MQ=255)
                                                gAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAg                                                                                                     >  1:1791677/1‑148 (MQ=255)
                                                 aatcttcaatcttcCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAAGAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGa                                                                                                    >  2:2517846/1‑148 (MQ=255)
                                                 aatcttcaatcttcCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAAGAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGa                                                                                                    >  2:735960/1‑148 (MQ=255)
                                                  atcttcaatcttcCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAAGAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGAt                                                                                                   >  2:2986872/1‑148 (MQ=255)
                                                      tcaatcttcCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGTTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTaaa                                                                                               >  2:2624221/1‑148 (MQ=255)
                                                      tcaatcttcCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTaaa                                                                                               <  2:1717451/148‑1 (MQ=255)
                                                      tcaatcttcCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTaaa                                                                                               <  2:1716769/148‑1 (MQ=255)
                                                      tcaatcttcCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTaaa                                                                                               >  2:248759/1‑148 (MQ=255)
                                                       caatcttcCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTaaaa                                                                                              <  2:2008102/148‑1 (MQ=255)
                                                        aatcttcCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAAGAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAAt                                                                                             <  2:1056810/148‑1 (MQ=255)
                                                        aatcttcCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAAGAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAAt                                                                                             <  1:734360/148‑1 (MQ=255)
                                                         atcttcCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAAGAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATa                                                                                            >  1:1287845/1‑148 (MQ=255)
                                                         atcttcCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAAGAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATa                                                                                            >  1:1288326/1‑148 (MQ=255)
                                                          tcttcCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATAc                                                                                           <  2:1638335/148‑1 (MQ=255)
                                                          tcttcCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATAc                                                                                           <  1:2439839/148‑1 (MQ=255)
                                                           cttcCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATAcc                                                                                          >  1:3450322/1‑148 (MQ=255)
                                                           cttcCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAAGAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATAcc                                                                                          <  2:455360/148‑1 (MQ=255)
                                                           cttcCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAAGAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATAcc                                                                                          >  1:2028679/1‑148 (MQ=255)
                                                             tcCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAAGAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCt                                                                                        >  1:3079700/1‑148 (MQ=255)
                                                                      aCTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGc                                                                               >  1:2260395/1‑148 (MQ=255)
                                                                      aCTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAAGAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGc                                                                               >  1:1120009/1‑148 (MQ=255)
                                                                       cTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAAGAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGcc                                                                              >  1:2599819/1‑148 (MQ=255)
                                                                       cTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAAGAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGcc                                                                              <  1:1997870/148‑1 (MQ=255)
                                                                         aCATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAg                                                                            >  2:3262072/1‑148 (MQ=255)
                                                                         aCATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAg                                                                            <  1:2213743/148‑1 (MQ=255)
                                                                             cacaATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGc                                                                        >  2:286236/1‑148 (MQ=255)
                                                                             cacaATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAAGAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGc                                                                        >  1:849743/1‑148 (MQ=255)
                                                                              acaATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGcc                                                                       <  2:2597243/148‑1 (MQ=255)
                                                                                aaTATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAAGAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAg                                                                     <  2:2028679/148‑1 (MQ=255)
                                                                                   atTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAAGAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGt                                                                  <  1:2309156/148‑1 (MQ=255)
                                                                                     tttATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAAGAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCg                                                                <  1:2480711/148‑1 (MQ=255)
                                                                                      ttATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGt                                                               <  1:3435781/148‑1 (MQ=255)
                                                                                      ttATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGt                                                               >  1:2490605/1‑148 (MQ=255)
                                                                                           cTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAAGAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACg                                                          >  2:2109248/1‑148 (MQ=255)
                                                                                           cTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAAGAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTGTACg                                                          >  2:316234/1‑148 (MQ=255)
                                                                                             ttCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTaa                                                                                    >  2:533422/1‑120 (MQ=255)
                                                                                             ttCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGtgttagtg                                                        >  1:644692/1‑141 (MQ=255)
                                                                                                aGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGa                                                     <  1:2072056/148‑1 (MQ=255)
                                                                                                  tGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGTTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGAcg                                                   <  1:2167172/148‑1 (MQ=255)
                                                                                                     aaTACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGa                                                >  2:2734126/1‑148 (MQ=255)
                                                                                                     aaTACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAAGAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGa                                                <  1:3188555/148‑1 (MQ=255)
                                                                                                      aTACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAAGAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGAt                                               >  2:1905053/1‑148 (MQ=255)
                                                                                                        aCGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAAGAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCa                                             >  2:394312/1‑148 (MQ=255)
                                                                                                          ggCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCACg                                           <  2:2809658/148‑1 (MQ=255)
                                                                                                            cGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAAGAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCACGCt                                         >  2:120208/1‑148 (MQ=255)
                                                                                                              ttAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGTCAGAAGCCAGGGTCGTGTTCGTGTGTCGCGCTCACGCTGc                                       >  1:3059237/1‑148 (MQ=255)
                                                                                                              ttAATGGCTAACACGCCCAGCAAACCCATAATAGAAAGAAGGGTTTCCATCACAGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTGTACGTGTGACGCGATCACGCTGc                                       >  1:2703761/1‑148 (MQ=255)
                                                                                                                  tGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTGTTAGTGTGACGCGATCACGCTGCCCGc                                   >  1:976125/1‑148 (MQ=255)
                                                                                                                  tGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTGTACGTGTGACGCGATCACGCTGCCCGc                                   >  1:2334788/1‑148 (MQ=255)
                                                                                                                   ggCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCACGCTGCCCGCa                                  <  1:2047891/148‑1 (MQ=255)
                                                                                                                      tAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCACGCTGCCCGCACCg                               >  1:1186003/1‑148 (MQ=255)
                                                                                                                       aaCACGCCCAGCAAACCCATAATAGACAGAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCACGCTGCCCGCACCgg                              <  2:287468/148‑1 (MQ=255)
                                                                                                                       aaCACGCCCAGCAAACCCATAATAGAAAGAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTGTACGTGTGTCGCGATCACGCTGCCCGCACCgg                              >  1:45183/1‑148 (MQ=255)
                                                                                                                         cacGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCACGCt                                         >  2:944738/1‑135 (MQ=255)
                                                                                                                         cacGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCACGCt                                         <  1:944738/135‑1 (MQ=255)
                                                                                                                           cGCCCAGCAAACCCATAATAGAAAGAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGc                          <  2:1120009/148‑1 (MQ=255)
                                                                                                                             cccAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGCCa                        <  1:286236/148‑1 (MQ=255)
                                                                                                                                aGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGCCCGTa                     >  2:3512608/1‑148 (MQ=255)
                                                                                                                                   aaaCCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGCCAGTACCa                  <  1:3403987/148‑1 (MQ=255)
                                                                                                                                            ataGAAAGAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGAAGCGATCACGCTGCCCGCACCCGTTGCCAGTACCATTAAAGCgg         >  2:1216840/1‑148 (MQ=255)
                                                                                                                                             taGAAAGAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGCCAGTACCATTAAAGCggg        <  1:2057304/148‑1 (MQ=255)
                                                                                                                                             taGAAAGAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCACCCTGCCCGCACCGGTTGCCAGTACCATTAAAGCggg        >  2:3040475/1‑148 (MQ=255)
                                                                                                                                             taGAAAGAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTGTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGCCAGTACCATTAAAGCggg        >  2:3039648/1‑148 (MQ=255)
                                                                                                                                              agaaagaaGGTTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGCCAGTACCATTAAAGCGGGa       <  1:2517846/148‑1 (MQ=255)
                                                                                                                                              agaaagaaGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGCCAGTACCATTAAAGCGGGa       <  1:735960/148‑1 (MQ=255)
                                                                                                                                               gAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTGTACGTGTGACGCGATCACGCGGCCCGCACCGGTTGCCAGTACCATTAAAGCgggtt      >  1:554182/1‑146 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGCCAGTACCATTAAAGCGGGATCGGC  >  CP009273/4480929‑4481223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑