Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,520,701 G→T 100% G256G (GGC→GGA)  sgcX ← putative endoglucanase with Zn‑dependent exopeptidase domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,520,7010GT95.8% 458.1 / 10.1 154G256G (GGC→GGA) sgcXputative endoglucanase with Zn‑dependent exopeptidase domain
Reads supporting (aligned to +/- strand):  ref base G (2/5);  new base T (73/74);  total (75/79)
Fisher's exact test for biased strand distribution p-value = 4.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 154 total aligned reads displayed.

CTGTTATCACGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATGCCGCGGATATTAAATTCTTCCTGTACCGAA  >  CP009273/4520556‑4520845
                                                                                                                                                 |                                                                                                                                                
 tGTTATCACGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTccc                                                                                                                                               <  2:1218073/148‑1 (MQ=255)
 tGTTATCACGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTccc                                                                                                                                               >  2:1780657/1‑148 (MQ=255)
  gTTATCACGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCt                                                                                                                                              <  2:493235/148‑1 (MQ=255)
      tCACGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGAtt                                                                                                                                          >  1:800009/1‑148 (MQ=255)
       cACGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTa                                                                                                                                         <  2:890276/148‑1 (MQ=255)
       cACGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTa                                                                                                                                         >  2:245514/1‑148 (MQ=255)
       cACGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTa                                                                                                                                         <  2:3206009/148‑1 (MQ=255)
       cACGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTa                                                                                                                                         >  1:1706916/1‑148 (MQ=255)
        aCGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTaa                                                                                                                                        <  1:1284391/148‑1 (MQ=255)
          gCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATc                                                                                                                                      <  2:2842181/148‑1 (MQ=255)
          gCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATc                                                                                                                                      <  1:580361/148‑1 (MQ=255)
           ccGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATcc                                                                                                                                     <  2:1823862/148‑1 (MQ=255)
              ggAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGa                                                                                                                                  <  1:3419980/148‑1 (MQ=255)
                aGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGAcc                                                                                                                                <  1:961143/148‑1 (MQ=255)
                    aCTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCgg                                                                                                                            <  2:2782257/148‑1 (MQ=255)
                      ttCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGaa                                                                                                                          <  1:741236/148‑1 (MQ=255)
                        ctcGCTGCACCGGAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGaata                                                                                                                        <  2:2809535/148‑1 (MQ=255)
                        ctcGCTGCACCGGAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGaata                                                                                                                        >  1:2960309/1‑148 (MQ=255)
                            cTGCACCGGAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCg                                                                                                                    <  1:1792876/148‑1 (MQ=255)
                            cTGCACCGGAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCg                                                                                                                    <  1:1324886/148‑1 (MQ=255)
                              gCACCGGAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCgtg                                                                                                                  >  1:475520/1‑148 (MQ=255)
                               cACCGGAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCgtgt                                                                                                                 >  1:1361315/1‑148 (MQ=255)
                               cACCGGAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCgtgt                                                                                                                 >  1:1361308/1‑148 (MQ=255)
                                  cGGAATGTTGTGTTCAAGAGCCGTCTGTTCCAACCTCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGAATAATCGTGTaaa                                                                                                              <  1:377809/148‑1 (MQ=255)
                                  cGGAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGAATAATCGTGTaaa                                                                                                              <  1:2167702/148‑1 (MQ=255)
                                       tgttgtGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAgg                                                                                                         >  2:157627/1‑148 (MQ=255)
                                         ttgtGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAgggg                                                                                                       >  2:685624/1‑148 (MQ=255)
                                         ttgtGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAgggg                                                                                                       >  1:2228610/1‑148 (MQ=255)
                                         ttgtGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAgggg                                                                                                       >  2:685603/1‑148 (MQ=255)
                                          tgtgTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGgt                                                                                                      >  1:663266/1‑148 (MQ=255)
                                              ttCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTca                                                                                                  <  1:1228214/148‑1 (MQ=255)
                                                cAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTcaca                                                                                                <  2:2265929/148‑1 (MQ=255)
                                                  agagCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACg                                                                                              <  2:1670521/148‑1 (MQ=255)
                                                   gagCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGa                                                                                             >  1:2889481/1‑148 (MQ=255)
                                                   gagCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGa                                                                                             >  1:498235/1‑148 (MQ=255)
                                                    agCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGAt                                                                                            >  2:1065219/1‑148 (MQ=255)
                                                    agCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGAt                                                                                            >  2:1065208/1‑148 (MQ=255)
                                                     gCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATg                                                                                           <  2:3278890/148‑1 (MQ=255)
                                                     gCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATg                                                                                           >  1:1963334/1‑148 (MQ=255)
                                                     gCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATg                                                                                           <  2:752141/148‑1 (MQ=255)
                                                     gCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATg                                                                                           >  1:2962736/1‑148 (MQ=255)
                                                       cGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGa                                                                                         >  2:1365802/1‑148 (MQ=255)
                                                       cGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGa                                                                                         >  1:56368/1‑148 (MQ=255)
                                                        gTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAg                                                                                        >  1:1865701/1‑148 (MQ=255)
                                                          cTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTg                                                                                      >  1:3236369/1‑148 (MQ=255)
                                                            gTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGat                                                                                    <  2:2423331/148‑1 (MQ=255)
                                                             ttCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGata                                                                                   <  2:444811/148‑1 (MQ=255)
                                                             gtCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGata                                                                                   <  2:444636/147‑1 (MQ=255)
                                                                 aaCATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCaa                                                                               >  2:3542660/1‑148 (MQ=255)
                                                                        gTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAAt                                                                        <  1:2158669/148‑1 (MQ=255)
                                                                            cAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGcc                                                                    <  1:890643/148‑1 (MQ=255)
                                                                             aGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCca                                                                   <  2:2574478/148‑1 (MQ=255)
                                                                             aGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCca                                                                   <  2:1740838/148‑1 (MQ=255)
                                                                              ggCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCcag                                                                  >  1:1570162/1‑148 (MQ=255)
                                                                               gCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCcagg                                                                 <  2:836821/148‑1 (MQ=255)
                                                                               gCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCcagg                                                                 <  2:836585/148‑1 (MQ=255)
                                                                               gCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATAAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGAcagg                                                                 >  1:611615/1‑148 (MQ=255)
                                                                                cGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCcaggt                                                                <  1:3427212/148‑1 (MQ=255)
                                                                                        cGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGtttt                                                        >  1:1081806/1‑148 (MQ=255)
                                                                                          tGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTac                                                      <  2:2916754/148‑1 (MQ=255)
                                                                                           gATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTaca                                                     <  2:3077896/148‑1 (MQ=255)
                                                                                                aTCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCg                                                <  2:3358982/148‑1 (MQ=255)
                                                                                                aTCCGGCCACCGGTCCCCGCCCATGGTAGTTCAGGCAGGTCATCCCAACTCCCTGAGTAATCCAGACCTCGGAATAATCGTGTAACACAGGGGTGTCACACGAAGGAGTGATATCACTACCAAGCGCCAGGACAGGTTTTACACGGCg                                                <  2:2024906/148‑1 (MQ=255)
                                                                                                  ccGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTa                                              <  1:746535/148‑1 (MQ=255)
                                                                                                   cGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTaa                                             <  2:2960309/148‑1 (MQ=255)
                                                                                                     gCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATa                                           <  1:2686010/148‑1 (MQ=255)
                                                                                                      ccAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATAc                                          >  1:849284/1‑148 (MQ=255)
                                                                                                      ccAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATAc                                          >  1:2252124/1‑148 (MQ=255)
                                                                                                         gCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACggg                                       >  1:1786685/1‑148 (MQ=255)
                                                                                                         gCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACggg                                       >  1:3388916/1‑148 (MQ=255)
                                                                                                            ttCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAAc                                    <  2:1977991/148‑1 (MQ=255)
                                                                                                             tCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACa                                   >  1:2432947/1‑148 (MQ=255)
                                                                                                             tCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACa                                   <  1:238603/148‑1 (MQ=255)
                                                                                                              ccccGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACaa                                  >  1:1147496/1‑148 (MQ=255)
                                                                                                               cccGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAAt                                 >  2:1276295/1‑148 (MQ=255)
                                                                                                                ccGACCCTGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATg                                <  2:3419884/148‑1 (MQ=255)
                                                                                                                ccGACCATGGTAGTTCAGGCAGGTGATCCCCACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCACTCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATg                                <  1:1744913/148‑1 (MQ=255)
                                                                                                                ccGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATg                                <  2:74804/148‑1 (MQ=255)
                                                                                                                ccGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATg                                <  2:1657156/148‑1 (MQ=255)
                                                                                                                 cGACCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATGc                               >  1:3268053/1‑148 (MQ=255)
                                                                                                                   aCCATGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATGCcg                             <  1:2620114/148‑1 (MQ=255)
                                                                                                                      aTGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATGCCGCgg                          >  1:361987/1‑148 (MQ=255)
                                                                                                                       tGGTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATGCCGCGGa                         >  1:2545245/1‑148 (MQ=255)
                                                                                                                         gTAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATGCCGCGGata                       <  1:2838411/148‑1 (MQ=255)
                                                                                                                          tAGTTCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATGCCGCGGatat                      >  1:3141658/1‑148 (MQ=255)
                                                                                                                            gTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATGCCGCGGATATTa                    >  1:3402254/1‑148 (MQ=255)
                                                                                                                             ttCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACTATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATGCCGCGGATATTaa                   >  1:883441/1‑148 (MQ=255)
                                                                                                                             ttCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGACCAATGCCGCGGATATTaa                   >  2:166458/1‑148 (MQ=255)
                                                                                                                              tCAGGCAGGTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATGCCGCGGATATTaaa                  <  2:169303/148‑1 (MQ=255)
                                                                                                                                 ggcaggTGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATGCCGCGGATATTAAAttc               <  2:1313444/148‑1 (MQ=255)
                                                                                                                                      gTGATCCCAACGCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATGCCGCGGATATTAAATTCTTCCt          <  1:82113/148‑1 (MQ=255)
                                                                                                                                       tGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATGCCGCGGATATTAAATTCTTCCTg         >  2:35341/1‑148 (MQ=255)
                                                                                                                                       tGATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATGCCGCGGATATTAAATTCTTCCTg         >  2:2929465/1‑148 (MQ=255)
                                                                                                                                        gATCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATGCCGCGGATATTAAATTCTTCCTGt        <  1:1997332/148‑1 (MQ=255)
                                                                                                                                         aTCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATGCCGCGGATATTAAATTCTTCCTGTa       <  1:328316/148‑1 (MQ=255)
                                                                                                                                          tCCCAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATGCCGCGGATATTAAATTCTTCCTGTAc      <  1:1076725/148‑1 (MQ=255)
                                                                                                                                           cccAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATGCCGCGGATATTAAATTCTTCCTGTAcc     >  2:492310/1‑148 (MQ=255)
                                                                                                                                            ccAACTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATGCCGCGGATATTAAATTCTTCCTGTACCg    <  1:969414/148‑1 (MQ=255)
                                                                                                                                              aaCTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATGCCGCGGATATTAAATTCTTCCTGTACCGaa  >  1:3460006/1‑148 (MQ=255)
                                                                                                                                              aaCTCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATGCCGCGGATATTAAATTCTTCCTGTACCGaa  <  1:1476407/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CTGTTATCACGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATGCCGCGGATATTAAATTCTTCCTGTACCGAA  >  CP009273/4520556‑4520845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑